Hit and run: transient deubiquitylase activity in a chromatin-remodeling complex.

abstract：:Human cleavage factor I(m) (CFI(m)) is a heterodimeric RNA binding protein complex that functions at an early step in the assembly of the pre-mRNA 3' processing complex. In this report we show that CFI(m) can stimulate both cleavage and poly(A) addition, and can act to suppress poly(A) site cleavage in a sequence-depe...

journal_title：Molecular cell

authors： Brown KM,Gilmartin GM

更新日期：2003-12-01 00:00:00

abstract：:The nuclear hormone receptor PPAR gamma promotes adipogenesis and macrophage differentiation and is a primary pharmacological target in the treatment of type II diabetes. Here, we show that PPAR gamma gene knockout results in two independent lethal phases. Initially, PPAR gamma deficiency interferes with terminal diff...

journal_title：Molecular cell

authors： Barak Y,Nelson MC,Ong ES,Jones YZ,Ruiz-Lozano P,Chien KR,Koder A,Evans RM

更新日期：1999-10-01 00:00:00

abstract：:The functional significance of mono-, di-, and trimethylation of lysine residues within histone proteins remains unclear. Antibodies developed to selectively recognize each of these methylated states at histone H3 lysine 9 (H3 Lys9) demonstrated that mono- and dimethylation localized specifically to silent domains wit...

journal_title：Molecular cell

authors： Rice JC,Briggs SD,Ueberheide B,Barber CM,Shabanowitz J,Hunt DF,Shinkai Y,Allis CD

更新日期：2003-12-01 00:00:00

abstract：:Transcription of telomeric DNA has long been thought to inhibit telomerase. Using live cell imaging, in this issue, Cusanelli et al. (2013) now reveal a different scenario: telomeric RNA is preferentially generated at short telomeres and delivers telomerase to the chromosome end from which the transcript originated. ...

journal_title：Molecular cell

authors： Smekalova E,Baumann P

更新日期：2013-09-26 00:00:00

abstract：:Transcription is a source of genetic instability that can notably result from the formation of genotoxic DNA:RNA hybrids, or R-loops, between the nascent mRNA and its template. Here we report an unexpected function for introns in counteracting R-loop accumulation in eukaryotic genomes. Deletion of endogenous introns i...

journal_title：Molecular cell

authors： Bonnet A,Grosso AR,Elkaoutari A,Coleno E,Presle A,Sridhara SC,Janbon G,Géli V,de Almeida SF,Palancade B

更新日期：2017-08-17 00:00:00

abstract：:The protective "cap" that assembles at chromosome ends recruits and controls an intricate network of biochemical activities, each one critical for telomere structure and the maintenance of genomic stability. Recent studies have uncovered the components of telomere caps and have started to define the pathways that lead...

journal_title：Molecular cell

authors： Ferreira MG,Miller KM,Cooper JP

更新日期：2004-01-16 00:00:00

abstract：:SPT5 and its binding partner SPT4 function in both positively and negatively regulating transcriptional elongation. The demonstration that SPT5 and RNA polymerase II are targets for phosphorylation by CDK9/cyclin T1 indicates that posttranslational modifications of these factors are important in regulating the elongat...

journal_title：Molecular cell

authors： Kwak YT,Guo J,Prajapati S,Park KJ,Surabhi RM,Miller B,Gehrig P,Gaynor RB

更新日期：2003-04-01 00:00:00

abstract：:We report an interaction between poxA, encoding a paralog of lysyl tRNA-synthetase, and the closely linked yjeK gene, encoding a putative 2,3-beta-lysine aminomutase, that is critical for virulence and stress resistance in Salmonella enterica. Salmonella poxA and yjeK mutants share extensive phenotypic pleiotropy, inc...

journal_title：Molecular cell

authors： Navarre WW,Zou SB,Roy H,Xie JL,Savchenko A,Singer A,Edvokimova E,Prost LR,Kumar R,Ibba M,Fang FC

更新日期：2010-07-30 00:00:00

abstract：:Mutations within BRCA1 predispose carriers to a high risk of breast and ovarian cancers. BRCA1 functions to maintain genomic stability through the assembly of multiple protein complexes involved in DNA repair, cell-cycle arrest, and transcriptional regulation. Here, we report the identification of a DNA damage-induced...

journal_title：Molecular cell

authors： Savage KI,Gorski JJ,Barros EM,Irwin GW,Manti L,Powell AJ,Pellagatti A,Lukashchuk N,McCance DJ,McCluggage WG,Schettino G,Salto-Tellez M,Boultwood J,Richard DJ,McDade SS,Harkin DP

更新日期：2014-05-08 00:00:00

abstract：:In vitro, the anaphase-promoting complex (APC) E3 ligase functions with E2 ubiquitin-conjugating enzymes of the E2-C and Ubc4/5 families to ubiquitinate substrates. However, only the use of the E2-C family, notably UbcH10, is genetically well validated. Here, we biochemically demonstrate preferential use of UbcH10 by ...

journal_title：Molecular cell

authors： Summers MK,Pan B,Mukhyala K,Jackson PK

更新日期：2008-08-22 00:00:00

abstract：:Caspase-7 is an obligate dimer of catalytic domains, with generation of activity requiring limited proteolysis within a region that separates the large and small chains of each domain. Using hybrid dimers we distinguish the relative contribution of each domain to catalysis by the whole molecule. We demonstrate that th...

journal_title：Molecular cell

authors： Denault JB,Békés M,Scott FL,Sexton KM,Bogyo M,Salvesen GS

更新日期：2006-08-01 00:00:00

abstract：:RNA-binding proteins coordinate the fates of multiple RNAs, but the principles underlying these global interactions remain poorly understood. We elucidated regulatory mechanisms of the RNA-binding protein HuR, by integrating data from diverse high-throughput targeting technologies, specifically PAR-CLIP, RIP-chip, and...

journal_title：Molecular cell

authors： Mukherjee N,Corcoran DL,Nusbaum JD,Reid DW,Georgiev S,Hafner M,Ascano M Jr,Tuschl T,Ohler U,Keene JD

更新日期：2011-08-05 00:00:00

abstract：:Spongiform encephalopathies are believed to be transmitted by a unique mechanism involving self-propagating conformational conversion of prion protein into a misfolded form. Here we demonstrate that fundamental aspects of mammalian prion propagation, including the species barrier and strain diversity, can be reproduce...

journal_title：Molecular cell

authors： Vanik DL,Surewicz KA,Surewicz WK

更新日期：2004-04-09 00:00:00

abstract：:Viral initiators perform multiple functions in initiation of DNA replication including ori binding, melting, and unwinding, culminating in the formation of a double hexameric (DH) helicase. We have recapitulated the assembly of the papillomavirus E1 initiator DH helicase, providing the first description of how such a ...

journal_title：Molecular cell

authors： Schuck S,Stenlund A

更新日期：2005-11-11 00:00:00

abstract：:Posttranslational histone modifications play important roles in transcription and other chromatin-based processes. Compared to acetylation, methylation, and phosphorylation, very little is known about the function of histone ubiquitylation. Here, we report the purification and functional characterization of a histone ...

journal_title：Molecular cell

authors： Wang H,Zhai L,Xu J,Joo HY,Jackson S,Erdjument-Bromage H,Tempst P,Xiong Y,Zhang Y

更新日期：2006-05-05 00:00:00

abstract：:Bursts of nascent mRNA have been shown to lead to substantial cell-cell variation in unicellular organisms, facilitating diverse responses to environmental challenges. It is unknown whether similar bursts and gene-expression noise occur in mammalian tissues. To address this, we combine single molecule transcript count...

journal_title：Molecular cell

authors： Bahar Halpern K,Tanami S,Landen S,Chapal M,Szlak L,Hutzler A,Nizhberg A,Itzkovitz S

更新日期：2015-04-02 00:00:00

abstract：:In this issue of Molecular Cell, Dango and Mosammaparast discover that the human oxidative demethylase ALKBH3 functions in complex with a DNA helicase to eliminate N3-methylcytosine lesions from ssDNA and that specific cancer cell lines are dependent on this activity for proliferation (Dango et al., 2011). ...

journal_title：Molecular cell

authors： Camps M,Eichman BF

更新日期：2011-11-04 00:00:00

abstract：:Rad51 is a DNA recombinase functioning in the repair of DNA double-strand breaks and the generation of genetic diversity by homologous recombination (HR). In the presence of ATP, Rad51 self-assembles into an extended polymer on single-stranded DNA to catalyze strand exchange. Inappropriate HR causes genomic instabilit...

journal_title：Molecular cell

authors： Antony E,Tomko EJ,Xiao Q,Krejci L,Lohman TM,Ellenberger T

更新日期：2009-07-10 00:00:00

abstract：:Both EZH2 and NF-κB contribute to aggressive breast cancer, yet whether the two oncogenic factors have functional crosstalk in breast cancer is unknown. Here, we uncover an unexpected role of EZH2 in conferring the constitutive activation of NF-κB target gene expression in ER-negative basal-like breast cancer cells. T...

journal_title：Molecular cell

authors： Lee ST,Li Z,Wu Z,Aau M,Guan P,Karuturi RK,Liou YC,Yu Q

更新日期：2011-09-02 00:00:00

abstract：:To determine whether replicational mutagenesis in the yeast genome is influenced by the positions of active origins, a reporter gene was placed in two orientations at multiple locations within a 39,000 bp region of chromosome III possessing two strong origins. The frequency of mutations resulting from misincorporation...

journal_title：Molecular cell

authors： Pavlov YI,Newlon CS,Kunkel TA

更新日期：2002-07-01 00:00:00

abstract：:In this issue of Molecular Cell, Huang et al. (2009) describe two heterotrimeric single-stranded DNA binding complexes, SOSS1 and SOSS2, that function downstream of the MRN complex to promote DNA repair and the G2/M checkpoint. ...

journal_title：Molecular cell

authors： Nam EA,Cortez D

更新日期：2009-08-14 00:00:00

abstract：:In this issue of Molecular Cell, Tripathi and coworkers (Tripathi et al., 2010) decode some of the functions of a long noncoding RNA MALAT1. They provide evidence that MALAT1 regulates alternative splicing by controlling the activity of the SR protein family of splicing factors. ...

journal_title：Molecular cell

authors： Ankö ML,Neugebauer KM

更新日期：2010-09-24 00:00:00

abstract：:Trimethylation of histone H3 on lysine 4 (H3K4me3) localizes near the 5' region of genes and is tightly associated with active loci. Several proteins, such as CHD1, BPTF, JMJD2A, and the ING tumor suppressor family, directly recognize this lysine methyl mark. However, how H3K4me3 recognition participates in active tra...

journal_title：Molecular cell

authors： Sims RJ 3rd,Millhouse S,Chen CF,Lewis BA,Erdjument-Bromage H,Tempst P,Manley JL,Reinberg D

更新日期：2007-11-30 00:00:00

abstract：:Ubiquitination typically occurs through the sequential action of three enzymes catalyzing ubiquitin activation (E1), conjugation (E2), and ligation (E3) and regulates diverse eukaryotic cellular processes. Although monoubiquitination commonly confers nondegradative activities, mechanisms underlying its temporal and sp...

journal_title：Molecular cell

authors： Sun L,Fang J

更新日期：2016-06-16 00:00:00

abstract：:The transcriptional coactivators CBP and p300 are critical regulators of metazoan gene expression. They associate with many different DNA-bound transcription factors through small, conserved domains. We have identified a compactly folded 46 residue domain in CBP and p300, the IRF-3 binding domain (IBiD), and we have d...

journal_title：Molecular cell

authors： Lin CH,Hare BJ,Wagner G,Harrison SC,Maniatis T,Fraenkel E

更新日期：2001-09-01 00:00:00

abstract：:MRE11 and RAD50 are known to be required for nonhomologous joining of DNA ends in vivo. We have investigated the enzymatic activities of the purified proteins and found that Mre11 by itself has 3' to 5' exonuclease activity that is increased when Mre11 is in a complex with Rad50. Mre11 also exhibits endonuclease activ...

journal_title：Molecular cell

authors： Paull TT,Gellert M

更新日期：1998-06-01 00:00:00

abstract：:In addition to protein-coding information, mRNAs harbor regulatory sequences necessary for appropriate processing of their precursors. Goren et al. (2006) and Wang et al. (2006) explore the diversity of these signals and the rules by which they function. ...

journal_title：Molecular cell

authors： Guigó R,Valcárcel J

更新日期：2006-07-21 00:00:00

abstract：:Solving the biological roles of covalent histone modifications, including monoubiquitination of histone H2A, and the molecular mechanisms by which these modifications regulate specific transcriptional programs remains a central question for all eukaryotes. Here we report that the N-CoR/HDAC1/3 complex specifically rec...

journal_title：Molecular cell

authors： Zhou W,Zhu P,Wang J,Pascual G,Ohgi KA,Lozach J,Glass CK,Rosenfeld MG

更新日期：2008-01-18 00:00:00

abstract：:The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas nuclease system is a powerful tool for genome editing, and its simple programmability has enabled high-throughput genetic and epigenetic studies. These high-throughput approaches offer investigators a toolkit for functional interrogation of not...

journal_title：Molecular cell

authors： Montalbano A,Canver MC,Sanjana NE

更新日期：2017-10-05 00:00:00

abstract：:Calreticulin and calnexin are homologous lectins that serve as molecular chaperones for glycoproteins in the endoplasmic reticulum of eukaryotic cells. Here we show that calreticulin depletion specifically accelerates the maturation of cellular and viral glycoproteins with a modest decrease in folding efficiency. Caln...

journal_title：Molecular cell

authors： Molinari M,Eriksson KK,Calanca V,Galli C,Cresswell P,Michalak M,Helenius A

更新日期：2004-01-16 00:00:00