Integrative regulatory mapping indicates that the RNA-binding protein HuR couples pre-mRNA processing and mRNA stability.

abstract：:The response to endoplasmic reticulum (ER) stress relies on activation of unfolded protein response (UPR) sensors, and the outcome of the UPR depends on the duration and strength of signal. Here, we demonstrate a mechanism that attenuates the activity of the UPR sensor inositol-requiring enzyme 1α (IRE1α). A resident ...

journal_title：Molecular cell

authors： Eletto D,Eletto D,Dersh D,Gidalevitz T,Argon Y

更新日期：2014-02-20 00:00:00

abstract：:Colicins kill E. coli by a process that involves binding to a surface receptor, entering the cell, and, finally, intoxicating it. The lethal action of colicin E3 is a specific cleavage in the ribosomal decoding A site. The crystal structure of colicin E3, reported here in a binary complex with its immunity protein (IP...

journal_title：Molecular cell

authors： Soelaiman S,Jakes K,Wu N,Li C,Shoham M

更新日期：2001-11-01 00:00:00

abstract：:The carboxy-terminal domain (CTD) of the large subunit of RNA polymerase II (Pol II) comprises multiple heptapeptide repeats of the consensus Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Reversible phosphorylation of Ser2, Ser5, and Ser7 during the transcription cycle mediates the sequential recruitment of transcription/RNA pr...

journal_title：Molecular cell

authors： Egloff S,Zaborowska J,Laitem C,Kiss T,Murphy S

更新日期：2012-01-13 00:00:00

abstract：:Among the five KCNQ channels, also known as the Kv7 voltage-gated potassium (Kv) channels, KCNQ2-KCNQ5 control neuronal excitability. Dysfunctions of KCNQ2-KCNQ5 are associated with neurological disorders such as epilepsy, deafness, and neuropathic pain. Here, we report the cryoelectron microscopy (cryo-EM) structures...

journal_title：Molecular cell

authors： Li T,Wu K,Yue Z,Wang Y,Zhang F,Shen H

更新日期：2021-01-07 00:00:00

abstract：:The protein kinase B-RAF is mutated in approximately 7% of human cancers. Most mutations are activating, but, surprisingly, a small number have reduced kinase activity. However, the latter can still stimulate cellular signaling through the MEK-ERK pathway because they activate the related family member C-RAF. We exami...

journal_title：Molecular cell

authors： Garnett MJ,Rana S,Paterson H,Barford D,Marais R

更新日期：2005-12-22 00:00:00

abstract：:PRDM16 is a transcription co-factor that plays critical roles in development of brown adipose tissue, as well as maintenance of adult hematopoietic and neural stem cells. Here we report that PRDM16 is a histone H3K4 methyltransferase on chromatin. Mutation in the N-terminal PR domain of PRDM16 abolishes the intrinsic ...

journal_title：Molecular cell

authors： Zhou B,Wang J,Lee SY,Xiong J,Bhanu N,Guo Q,Ma P,Sun Y,Rao RC,Garcia BA,Hess JL,Dou Y

更新日期：2016-04-21 00:00:00

abstract：:Cohesion between sister chromatids is mediated by the chromosomal cohesin complex. In budding yeast, cohesin is loaded onto chromosomes during the G1 phase of the cell cycle. During S phase, the replication fork-associated acetyltransferase Eco1 acetylates the cohesin subunit Smc3 to promote the establishment of siste...

journal_title：Molecular cell

authors： Borges V,Lehane C,Lopez-Serra L,Flynn H,Skehel M,Rolef Ben-Shahar T,Uhlmann F

更新日期：2010-09-10 00:00:00

abstract：:Protein phase separation by low-complexity, intrinsically disordered domains generates membraneless organelles and links to neurodegeneration. Cellular prion protein (PrPC) contains such domains, causes spongiform degeneration, and is a receptor for Alzheimer's amyloid-β oligomers (Aβo). Here, we show that PrPC separa...

journal_title：Molecular cell

authors： Kostylev MA,Tuttle MD,Lee S,Klein LE,Takahashi H,Cox TO,Gunther EC,Zilm KW,Strittmatter SM

更新日期：2018-11-01 00:00:00

abstract：:In all branches of life, stalled translation intermediates are recognized and processed by ribosome-associated quality control (RQC) pathways. RQC begins with the splitting of stalled ribosomes, leaving an unfinished polypeptide still attached to the large subunit. Ancient and conserved NEMF family RQC proteins target...

journal_title：Molecular cell

authors： Crowe-McAuliffe C,Takada H,Murina V,Polte C,Kasvandik S,Tenson T,Ignatova Z,Atkinson GC,Wilson DN,Hauryliuk V

更新日期：2021-01-07 00:00:00

abstract：:Productive splicing of human precursor messenger RNAs (pre-mRNAs) requires the correct selection of authentic splice sites (SS) from the large pool of potential SS. Although SS consensus sequence and splicing regulatory proteins are known to influence SS usage, the mechanisms ensuring the effective suppression of cryp...

journal_title：Molecular cell

authors： Boehm V,Britto-Borges T,Steckelberg AL,Singh KK,Gerbracht JV,Gueney E,Blazquez L,Altmüller J,Dieterich C,Gehring NH

更新日期：2018-11-01 00:00:00

abstract：:Centromeres play several important roles in ensuring proper chromosome segregation. Not only do they promote kinetochore assembly for microtubule attachment, but they also support robust sister chromatid cohesion at pericentromeres and facilitate replication of centromeric DNA early in S phase. However, it is still el...

journal_title：Molecular cell

authors： Natsume T,Müller CA,Katou Y,Retkute R,Gierliński M,Araki H,Blow JJ,Shirahige K,Nieduszynski CA,Tanaka TU

更新日期：2013-06-06 00:00:00

abstract：:During transcription initiation by bacterial RNA polymerase, the sigma subunit recognizes the -35 and -10 promoter elements; free sigma, however, does not bind DNA. We selected ssDNA aptamers that strongly and specifically bound free sigma(A) from Thermus aquaticus. A consensus sequence, GTA(C/T)AATGGGA, was required ...

journal_title：Molecular cell

authors： Feklistov A,Barinova N,Sevostyanova A,Heyduk E,Bass I,Vvedenskaya I,Kuznedelov K,Merkiene E,Stavrovskaya E,Klimasauskas S,Nikiforov V,Heyduk T,Severinov K,Kulbachinskiy A

更新日期：2006-07-07 00:00:00

abstract：:Eukaryotic neurotransmitter:sodium symporters (NSSs), targets for antidepressants and psychostimulants, terminate neurotransmission by sodium-driven reuptake. The crystal structure of LeuT(Aa), a prokaryotic NSS homolog, revealed an occluded state in which one leucine and two Na(+) ions are bound, but provided limited...

journal_title：Molecular cell

authors： Shi L,Quick M,Zhao Y,Weinstein H,Javitch JA

更新日期：2008-06-20 00:00:00

abstract：:The transcriptional activity of many sequence-specific DNA binding proteins is directly regulated by posttranslational covalent modification. Although this form of regulation was first described nearly two decades ago, it remains poorly understood at a mechanistic level. The prototype for a transcription factor contro...

journal_title：Molecular cell

authors： He C,Hus JC,Sun LJ,Zhou P,Norman DP,Dötsch V,Wei H,Gross JD,Lane WS,Wagner G,Verdine GL

更新日期：2005-10-07 00:00:00

abstract：:Mammalian target of rapamycin (mTOR) activity is regulated by assembly of two functionally distinct complexes, mTORC1 and mTORC2. In syndecan-4 (S4) null endothelial cells, mTORC2 activity is reduced, resulting in decreased Akt activation, while mTORC1 activity is increased. Levels of rictor, mLST8, and mSin-1 are unc...

journal_title：Molecular cell

authors： Partovian C,Ju R,Zhuang ZW,Martin KA,Simons M

更新日期：2008-10-10 00:00:00

abstract：:CRISPR-Cas immunity requires integration of short, foreign DNA fragments into the host genome at the CRISPR locus, a site consisting of alternating repeat sequences and foreign-derived spacers. In most CRISPR systems, the proteins Cas1 and Cas2 form the integration complex and are both essential for DNA acquisition. M...

journal_title：Molecular cell

authors： Wright AV,Wang JY,Burstein D,Harrington LB,Paez-Espino D,Kyrpides NC,Iavarone AT,Banfield JF,Doudna JA

更新日期：2019-02-21 00:00:00

abstract：:The liver plays a central role in the control of glucose homeostasis and is subject to complex regulation by substrates, insulin, and other hormones. To investigate the effect of the loss of direct insulin action in liver, we have used the Cre-loxP system to inactivate the insulin receptor gene in hepatocytes. Liver-s...

journal_title：Molecular cell

authors： Michael MD,Kulkarni RN,Postic C,Previs SF,Shulman GI,Magnuson MA,Kahn CR

更新日期：2000-07-01 00:00:00

abstract：:Canonical Wnt signaling is thought to regulate cell behavior mainly by inducing β-catenin-dependent transcription of target genes. In proliferating cells Wnt signaling peaks in the G2/M phase of the cell cycle, but the significance of this "mitotic Wnt signaling" is unclear. Here we introduce Wnt-dependent stabilizati...

journal_title：Molecular cell

authors： Acebron SP,Karaulanov E,Berger BS,Huang YL,Niehrs C

更新日期：2014-05-22 00:00:00

abstract：:In a recent issue of Molecular Cell, Jia et al. (2008) demonstrate that time-controlled repression of interferon-induced transcripts depends upon the interaction between an RNA structure in these transcripts and protein domains harbored by a mammalian aminoacyl-tRNA synthetase. ...

journal_title：Molecular cell

authors： Ribas de Pouplana L,Geslain R

更新日期：2008-04-11 00:00:00

abstract：:The molecular structures of crossover (CO) and noncrossover (NCO) intermediates were determined by sequencing the products formed when a gapped plasmid was repaired using a diverged chromosomal template. Analyses were done in the absence of mismatch repair (MMR) to allow efficient detection of strand-transfer intermed...

journal_title：Molecular cell

authors： Mitchel K,Zhang H,Welz-Voegele C,Jinks-Robertson S

更新日期：2010-04-23 00:00:00

abstract：:In vitro, the anaphase-promoting complex (APC) E3 ligase functions with E2 ubiquitin-conjugating enzymes of the E2-C and Ubc4/5 families to ubiquitinate substrates. However, only the use of the E2-C family, notably UbcH10, is genetically well validated. Here, we biochemically demonstrate preferential use of UbcH10 by ...

journal_title：Molecular cell

authors： Summers MK,Pan B,Mukhyala K,Jackson PK

更新日期：2008-08-22 00:00:00

abstract：:The RNA-binding protein HuR, while known to stabilize cytoplasmic mRNAs, is largely nuclear. In this issue of Molecular Cell, Mukherjee et al. (2011) and Lebedeva et al. (2011) identify transcriptome-wide HuR-RNA interactions using PAR-CLIP, unveiling HuR's nuclear role in pre-mRNA processing. ...

journal_title：Molecular cell

authors： Srikantan S,Gorospe M

更新日期：2011-08-05 00:00:00

abstract：:Nucleosome loss from a promoter region has recently been described as a potential mechanism for transcriptional regulation. We investigated whether H3/H4 histone chaperones mediate the loss of nucleosomes from the promoter of the yeast PHO5 gene during transcriptional activation. We found that antisilencing function 1...

journal_title：Molecular cell

authors： Adkins MW,Howar SR,Tyler JK

更新日期：2004-06-04 00:00:00

abstract：:Inappropriate activation of oncogenic kinases at intracellular locations is frequently observed in human cancers, but its effects on global signaling are incompletely understood. Here, we show that the oncogenic mutant of Flt3 (Flt3-ITD), when localized at the endoplasmic reticulum (ER), aberrantly activates STAT5 and...

journal_title：Molecular cell

authors： Choudhary C,Olsen JV,Brandts C,Cox J,Reddy PN,Böhmer FD,Gerke V,Schmidt-Arras DE,Berdel WE,Müller-Tidow C,Mann M,Serve H

更新日期：2009-10-23 00:00:00

abstract：:Transcription initiation requires opening of promoter DNA in the RNA polymerase II (Pol II) pre-initiation complex (PIC), but it remains unclear how this is achieved. Here we report the cryo-electron microscopic (cryo-EM) structure of a yeast PIC that contains underwound, distorted promoter DNA in the closed Pol II cl...

journal_title：Molecular cell

authors： Dienemann C,Schwalb B,Schilbach S,Cramer P

更新日期：2019-01-03 00:00:00

abstract：:In bacteria, RNA polymerase (RNAP) initiates transcription by synthesizing short transcripts that are either released or extended to allow RNAP to escape from the promoter. The mechanism of initial transcription is unclear due to the presence of transient intermediates and molecular heterogeneity. Here, we studied ini...

journal_title：Molecular cell

authors： Duchi D,Bauer DL,Fernandez L,Evans G,Robb N,Hwang LC,Gryte K,Tomescu A,Zawadzki P,Morichaud Z,Brodolin K,Kapanidis AN

更新日期：2016-09-15 00:00:00

abstract：:The DNA polymerase from phage phi29 is a B family polymerase that initiates replication using a protein as a primer, attaching the first nucleotide of the phage genome to the hydroxyl of a specific serine of the priming protein. The crystal structure of phi29 DNA polymerase determined at 2.2 A resolution provides expl...

journal_title：Molecular cell

authors： Kamtekar S,Berman AJ,Wang J,Lázaro JM,de Vega M,Blanco L,Salas M,Steitz TA

更新日期：2004-11-19 00:00:00

abstract：:Metazoan transcription factors typically regulate large numbers of genes. Here we identify via a CRISPR-Cas9 genetic screen ZNF410, a pentadactyl DNA-binding protein that in human erythroid cells directly activates only a single gene, the NuRD component CHD4. Specificity is conveyed by two highly evolutionarily conser...

journal_title：Molecular cell

authors： Lan X,Ren R,Feng R,Ly LC,Lan Y,Zhang Z,Aboreden N,Qin K,Horton JR,Grevet JD,Mayuranathan T,Abdulmalik O,Keller CA,Giardine B,Hardison RC,Crossley M,Weiss MJ,Cheng X,Shi J,Blobel GA

更新日期：2021-01-21 00:00:00

abstract：:The eukaryotic RNA exosome is an essential and conserved 3'-to-5' exoribonuclease complex that degrades or processes nearly every class of cellular RNA. The nuclear RNA exosome includes a 9-subunit non-catalytic core that binds Rrp44 (Dis3) and Rrp6 subunits to modulate their processive and distributive 3'-to-5' exori...

journal_title：Molecular cell

authors： Zinder JC,Wasmuth EV,Lima CD

更新日期：2016-11-17 00:00:00

abstract：:The duplication of eukaryotic genomes involves the replication of DNA from multiple origins of replication. In S phase, two sister replisomes assemble at each active origin, and they replicate DNA in opposite directions. Little is known about the functional relationship between sister replisomes. Some data imply that ...

journal_title：Molecular cell

authors： Yardimci H,Loveland AB,Habuchi S,van Oijen AM,Walter JC

更新日期：2010-12-10 00:00:00