Promoter Distortion and Opening in the RNA Polymerase II Cleft.

abstract：:A kinase-anchoring proteins (AKAPs) target PKA to specific microdomains by using an amphipathic helix that docks to N-terminal dimerization and docking (D/D) domains of PKA regulatory (R) subunits. To understand specificity, we solved the crystal structure of the helical motif from D-AKAP2, a dual-specific AKAP, bound...

journal_title：Molecular cell

authors： Kinderman FS,Kim C,von Daake S,Ma Y,Pham BQ,Spraggon G,Xuong NH,Jennings PA,Taylor SS

更新日期：2006-11-03 00:00:00

abstract：:The displacement loop (D loop) is the product of homology search and DNA strand invasion, constituting a central intermediate in homologous recombination (HR). In eukaryotes, the Rad51 DNA strand exchange protein is assisted in D loop formation by the Rad54 motor protein. Curiously, Rad54 also disrupts D loops. How th...

journal_title：Molecular cell

authors： Wright WD,Heyer WD

更新日期：2014-02-06 00:00:00

abstract：:Multisite phosphorylation of kinases can induce on-off or graded regulation of catalytic activity; however, its influence on substrate specificity remains unclear. Here, we show that multisite phosphorylation of ribosomal protein S6 kinase 1 (S6K1) alters target selection. Agonist-inducible phosphorylation of glutamyl...

journal_title：Molecular cell

authors： Arif A,Jia J,Willard B,Li X,Fox PL

更新日期：2019-02-07 00:00:00

abstract：:Progressive phosphorylation of circadian clock proteins is a hallmark of time-keeping. In this issue of Molecular Cell, Querfurth et al. (2011) demonstrate that phosphorylation of Neurospora FRQ induces a conformational change, which can account for its temporally gated degradation. ...

journal_title：Molecular cell

authors： Menet JS,Rosbash M

更新日期：2011-09-02 00:00:00

abstract：:The dynamic protein interactions required for transcription are functionally important yet poorly understood; in this issue of Molecular Cell, Zobeck et al. (2010) resolve the sequential recruitment and selective recycling of transcription factors at an actively transcribing locus in Drosophila. ...

journal_title：Molecular cell

authors： Motta-Mena LB,Partch CL,Gardner KH

更新日期：2010-12-22 00:00:00

abstract：:In this issue of Molecular Cell, Chen et al. (2014) provide evidence that FMRP represses translation by binding the ribosome, suggesting a novel form of translational control. ...

journal_title：Molecular cell

authors： Harigaya Y,Parker R

更新日期：2014-05-08 00:00:00

abstract：:The IkappaB kinases IKKalpha and IKKbeta regulate distinct cytoplasmic and nuclear events that are critical for cytokine-mediated activation of the NF-kappaB pathway. Because the IKKs have previously been demonstrated to associate with the nuclear hormone receptor coactivator AIB1/SRC-3, the question of whether either...

journal_title：Molecular cell

authors： Park KJ,Krishnan V,O'Malley BW,Yamamoto Y,Gaynor RB

更新日期：2005-04-01 00:00:00

abstract：:Cellular stress results in profound changes in RNA and protein synthesis. How cells integrate this intrinsic, p53-centered program with extracellular signals is largely unknown. We demonstrate that TGF-β1 signaling interferes with the stress response through coordinate transcriptional and translational repression of p...

journal_title：Molecular cell

authors： López-Díaz FJ,Gascard P,Balakrishnan SK,Zhao J,Del Rincon SV,Spruck C,Tlsty TD,Emerson BM

更新日期：2013-05-23 00:00:00

abstract：:An important paper by David Allis and colleagues (Ahn et al., 2006 [this issue of Molecular Cell]) describes a specific deacetylation/phosphorylation crosstalk at the histone H2B tail required for apoptosis induction in yeast, thus giving first insights into the poorly understood epigenetic regulation of cell death. ...

journal_title：Molecular cell

authors： Carmona-Gutierrez D,Madeo F

更新日期：2006-10-20 00:00:00

abstract：:RNA molecules play diverse functional roles in natural biological systems. There has been growing interest in designing synthetic RNA counterparts for programming biological function. The design of synthetic RNA molecules that exhibit diverse activities, including sensing, regulatory, information processing, and scaff...

journal_title：Molecular cell

authors： Liang JC,Bloom RJ,Smolke CD

更新日期：2011-09-16 00:00:00

abstract：:Stress granules (SGs) are condensates of mRNPs that form in response to stress. SGs arise by multivalent protein-protein, protein-RNA, and RNA-RNA interactions. However, the role of RNA-RNA interactions in SG assembly remains understudied. Here, we describe a yeast SG reconstitution system that faithfully recapitulate...

journal_title：Molecular cell

authors： Begovich K,Wilhelm JE

更新日期：2020-09-17 00:00:00

abstract：:Proteins with annealing activity are newly identified ATP-dependent motors that can rewind RPA-coated complementary single-stranded DNA bubbles. AH2 (annealing helicase 2, also named as ZRANB3) is the second protein with annealing activity, the function of which is still unknown. Here, we report that AH2 is recruited ...

journal_title：Molecular cell

authors： Yuan J,Ghosal G,Chen J

更新日期：2012-08-10 00:00:00

abstract：:In a recent issue of Molecular Cell, Jia et al. (2008) demonstrate that time-controlled repression of interferon-induced transcripts depends upon the interaction between an RNA structure in these transcripts and protein domains harbored by a mammalian aminoacyl-tRNA synthetase. ...

journal_title：Molecular cell

authors： Ribas de Pouplana L,Geslain R

更新日期：2008-04-11 00:00:00

abstract：:Polyubiquitination is a posttranslational modification where ubiquitin chains containing isopeptide bonds linking one of seven ubiquitin lysines with the C terminus of an adjoining ubiquitin are covalently attached to proteins. While functions of K48- and K63-linked polyubiquitin are understood, the role(s) of noncano...

journal_title：Molecular cell

authors： Matsumoto ML,Wickliffe KE,Dong KC,Yu C,Bosanac I,Bustos D,Phu L,Kirkpatrick DS,Hymowitz SG,Rape M,Kelley RF,Dixit VM

更新日期：2010-08-13 00:00:00

abstract：:Corticotropin-releasing factor (CRF) and the three related peptides urocortins 1-3 (UCN1-UCN3) are endocrine hormones that control the stress responses by activating CRF1R and CRF2R, two members of class B G-protein-coupled receptors (GPCRs). Here, we present two cryoelectron microscopy (cryo-EM) structures of UCN1-bo...

journal_title：Molecular cell

authors： Ma S,Shen Q,Zhao LH,Mao C,Zhou XE,Shen DD,de Waal PW,Bi P,Li C,Jiang Y,Wang MW,Sexton PM,Wootten D,Melcher K,Zhang Y,Xu HE

更新日期：2020-02-06 00:00:00

abstract：:Diaphanous-related formins (DRFs) regulate dynamics of unbranched actin filaments during cell contraction and cytokinesis. DRFs are autoinhibited through intramolecular binding of a Diaphanous autoinhibitory domain (DAD) to a conserved N-terminal regulatory element. Autoinhibition is relieved through binding of the GT...

journal_title：Molecular cell

authors： Otomo T,Otomo C,Tomchick DR,Machius M,Rosen MK

更新日期：2005-04-29 00:00:00

abstract：:In mammals, inactivation of one X chromosome in the female equalizes gene dosages between XX females and XY males. Two noncoding loci, Tsix and Xite, together regulate X chromosome fate by controlling homologous chromosome pairing, counting, and mutually exclusive choice. Following choice, the asymmetry of Xite and Ts...

journal_title：Molecular cell

authors： Donohoe ME,Zhang LF,Xu N,Shi Y,Lee JT

更新日期：2007-01-12 00:00:00

abstract：:In this issue of Molecular Cell, Chereji et al. (2017) present new data on MNase-sensitive particles previously identified upstream of transcription start sites at many promoters in budding yeast, and they argue, based upon negative histone-ChIP results, that they are non-nucleosomal signals generated by transcription...

journal_title：Molecular cell

authors： Kubik S,Bruzzone MJ,Albert B,Shore D

更新日期：2017-02-02 00:00:00

abstract：:In eukaryotes, three-dimensional genome organization is critical for transcriptional regulation of gene expression. Long noncoding RNAs (lncRNAs) can modulate chromatin conformation of spatially related genomic locations within the nucleus. Here, we show that the lncRNA APOLO (AUXIN-REGULATED PROMOTER LOOP) recognizes...

journal_title：Molecular cell

authors： Ariel F,Lucero L,Christ A,Mammarella MF,Jegu T,Veluchamy A,Mariappan K,Latrasse D,Blein T,Liu C,Benhamed M,Crespi M

更新日期：2020-03-05 00:00:00

abstract：:In this issue of Molecular Cell, Lu et al. (2019) analyze the role of the length and sequence complexity of the RNA polymerase II unstructured C-terminal domain in animal viability, development, and the dynamics of RNA polymerase II in vivo. ...

journal_title：Molecular cell

authors： Isaac RS,Churchman LS

更新日期：2019-03-21 00:00:00

abstract：:In eukaryotes, a crucial step of translation initiation is the binding of the multifactor complex eIF4F to the 5' end of the mRNA, a prerequisite to recruitment of the activated small ribosomal 43S particle. Histone H4 mRNAs have short 5'UTRs, which do not conform to the conventional scanning-initiation model. Here we...

journal_title：Molecular cell

authors： Martin F,Barends S,Jaeger S,Schaeffer L,Prongidi-Fix L,Eriani G

更新日期：2011-01-21 00:00:00

abstract：:The recent paper by Stadlmann et al. (2017) provides a novel algorithm for glycoproteomics in which complex glycopeptides can be identified in complex mixtures to aid in characterizing both the site of glycosylation and the glycan structure. ...

journal_title：Molecular cell

authors： Cummings RD

更新日期：2017-12-07 00:00:00

abstract：:MRE11 and RAD50 are known to be required for nonhomologous joining of DNA ends in vivo. We have investigated the enzymatic activities of the purified proteins and found that Mre11 by itself has 3' to 5' exonuclease activity that is increased when Mre11 is in a complex with Rad50. Mre11 also exhibits endonuclease activ...

journal_title：Molecular cell

authors： Paull TT,Gellert M

更新日期：1998-06-01 00:00:00

abstract：:B and T cell receptor gene assembly by V(D)J recombination is tightly regulated during lymphoid development. The mechanisms involved in this regulation are poorly understood. Here we show that nucleosomal DNA is refractory to V(D)J cleavage. However, the presence of HMG1, a chromatin-associated nonhistone DNA-binding ...

journal_title：Molecular cell

authors： Kwon J,Imbalzano AN,Matthews A,Oettinger MA

更新日期：1998-12-01 00:00:00

abstract：:The duplication of eukaryotic genomes involves the replication of DNA from multiple origins of replication. In S phase, two sister replisomes assemble at each active origin, and they replicate DNA in opposite directions. Little is known about the functional relationship between sister replisomes. Some data imply that ...

journal_title：Molecular cell

authors： Yardimci H,Loveland AB,Habuchi S,van Oijen AM,Walter JC

更新日期：2010-12-10 00:00:00

abstract：:Both H4K16 acetylation and H3K4 trimethylation are required for gene activation. However, it is still largely unclear how these modifications are orchestrated by transcriptional factors. Here, we analyzed the mechanism of the transcriptional activation by FOXP3, an X-linked suppressor of autoimmune diseases and cancer...

journal_title：Molecular cell

authors： Katoh H,Qin ZS,Liu R,Wang L,Li W,Li X,Wu L,Du Z,Lyons R,Liu CG,Liu X,Dou Y,Zheng P,Liu Y

更新日期：2011-12-09 00:00:00

abstract：:RanBP2/Nup358 is an essential protein with roles in nuclear transport and mitosis, and is one of the few known SUMO E3 ligases. However, why RanBP2 functions in vivo has been unclear: throughout the cell cycle it stably interacts with RanGAP1*SUMO1 and Ubc9, whose binding sites overlap with the E3 ligase region. Here ...

journal_title：Molecular cell

authors： Werner A,Flotho A,Melchior F

更新日期：2012-05-11 00:00:00

abstract：:Cohesion between sister chromatids is mediated by the chromosomal cohesin complex. In budding yeast, cohesin is loaded onto chromosomes during the G1 phase of the cell cycle. During S phase, the replication fork-associated acetyltransferase Eco1 acetylates the cohesin subunit Smc3 to promote the establishment of siste...

journal_title：Molecular cell

authors： Borges V,Lehane C,Lopez-Serra L,Flynn H,Skehel M,Rolef Ben-Shahar T,Uhlmann F

更新日期：2010-09-10 00:00:00

abstract：:Macroautophagy (autophagy) is a lysosome-dependent degradation process that has been implicated in age-associated diseases. Autophagy is involved in both cell survival and cell death, but little is known about the mechanisms that distinguish its use during these distinct cell fates. Here, we identify the microRNA miR-...

journal_title：Molecular cell

authors： Nelson C,Ambros V,Baehrecke EH

更新日期：2014-11-06 00:00:00

abstract：:Serine-arginine (SR) proteins are general splicing factors and can function through binding to exonic splicing enhancers (ESEs). SR proteins and several other mammalian splicing factors contain an arginine-serine-rich (RS) domain required to promote splicing. We have recently found that the ESE bound RS domain functio...

journal_title：Molecular cell

authors： Shen H,Green MR

更新日期：2004-11-05 00:00:00