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Wild-type and mutant B-RAF activate C-RAF through distinct mechanisms involving heterodimerization.

Abstract：

:The protein kinase B-RAF is mutated in approximately 7% of human cancers. Most mutations are activating, but, surprisingly, a small number have reduced kinase activity. However, the latter can still stimulate cellular signaling through the MEK-ERK pathway because they activate the related family member C-RAF. We examine the mechanism underlying C-RAF activation by B-RAF. We show that C-RAF is activated in the cytosol in a RAS-independent manner that requires activation segment phosphorylation and binding of 14-3-3 to C-RAF. We show that wild-type B-RAF forms a complex with C-RAF in a RAS-dependent manner, whereas the mutants bind independently of RAS. Importantly, we show that wild-type B-RAF can also activate C-RAF. Our data suggest that B-RAF activates C-RAF through a mechanism involving 14-3-3 mediated heterooligomerization and C-RAF transphosphorylation. Thus, we have identified a B-RAF-C-RAF-MEK-ERK cascade that signals not only in cancer but also in normal cells.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Garnett MJ,Rana S,Paterson H,Barford D,Marais R

doi

10.1016/j.molcel.2005.10.022

subject

Has Abstract

pub_date

2005-12-22 00:00:00

pages

963-9

issue

6

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(05)01717-X

journal_volume

20

pub_type

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