Abstract：
:The LTR-retrotransposon Tf1 preserves the coding capacity of its host Schizosaccharomyces pombe by integrating upstream of open reading frames (ORFs). To determine which features of the target sites were recognized by the transposon, we introduced plasmids containing candidate insertion sites into S. pombe and mapped the positions of integration. We found that Tf1 was targeted specifically to the promoters of Pol II-transcribed genes. A detailed analysis of integration in plasmids that contained either ade6 or fbp1 revealed insertions occurred in the promoters at positions where transcription factors bound. Further experiments revealed that the activator Atf1p and its binding site were required for directing integration to the promoter of fbp1. An interaction between Tf1 integrase and Atf1p was observed, indicating that integration at fbp1 was mediated by the activator bound to its promoter. Surprisingly, we found Tf1 contained sequences that activated transcription, and these substituted for elements of the ade6 promoter disrupted by integration.
journal_name
Mol Celljournal_title
Molecular cellauthors
Leem YE,Ripmaster TL,Kelly FD,Ebina H,Heincelman ME,Zhang K,Grewal SI,Hoffman CS,Levin HLdoi
10.1016/j.molcel.2008.02.016subject
Has Abstractpub_date
2008-04-11 00:00:00pages
98-107issue
1eissn
1097-2765issn
1097-4164pii
S1097-2765(08)00135-4journal_volume
30pub_type
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