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Uncoupling of sister replisomes during eukaryotic DNA replication.

Abstract：

:The duplication of eukaryotic genomes involves the replication of DNA from multiple origins of replication. In S phase, two sister replisomes assemble at each active origin, and they replicate DNA in opposite directions. Little is known about the functional relationship between sister replisomes. Some data imply that they travel away from one another and thus function independently. Alternatively, sister replisomes may form a stationary, functional unit that draws parental DNA toward itself. If this "double replisome" model is correct, a constrained DNA molecule should not undergo replication. To test this prediction, lambda DNA was stretched and immobilized at both ends within a microfluidic flow cell. Upon exposure to Xenopus egg extracts, this DNA underwent extensive replication by a single pair of diverging replisomes. The data show that there is no obligatory coupling between sister replisomes and, together with other studies, imply that genome duplication involves autonomously functioning replisomes.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Yardimci H,Loveland AB,Habuchi S,van Oijen AM,Walter JC

doi

10.1016/j.molcel.2010.11.027

subject

Has Abstract

pub_date

2010-12-10 00:00:00

pages

834-40

issue

5

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(10)00893-2

journal_volume

40

pub_type

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