Negative regulation of the mammalian UV response by Myc through association with Miz-1.

abstract：:Transcription is a source of genetic instability that can notably result from the formation of genotoxic DNA:RNA hybrids, or R-loops, between the nascent mRNA and its template. Here we report an unexpected function for introns in counteracting R-loop accumulation in eukaryotic genomes. Deletion of endogenous introns i...

journal_title：Molecular cell

authors： Bonnet A,Grosso AR,Elkaoutari A,Coleno E,Presle A,Sridhara SC,Janbon G,Géli V,de Almeida SF,Palancade B

更新日期：2017-08-17 00:00:00

abstract：:The steroid hormone ecdysone signals the stage-specific programmed cell death of the larval salivary glands during Drosophila metamorphosis. This response is preceded by an ecdysone-triggered switch in gene expression in which the diap2 death inhibitor is repressed and the reaper (rpr) and head involution defective (h...

journal_title：Molecular cell

authors： Jiang C,Lamblin AF,Steller H,Thummel CS

更新日期：2000-03-01 00:00:00

abstract：:Pilin assembly into type IV pili is required for virulence by bacterial pathogens that cause diseases such as cholera, pneumonia, gonorrhea, and meningitis. Crystal structures of soluble, N-terminally truncated pilin from Vibrio cholera toxin-coregulated pilus (TCP) and full-length PAK pilin from Pseudomonas aeruginos...

journal_title：Molecular cell

authors： Craig L,Taylor RK,Pique ME,Adair BD,Arvai AS,Singh M,Lloyd SJ,Shin DS,Getzoff ED,Yeager M,Forest KT,Tainer JA

更新日期：2003-05-01 00:00:00

abstract：:The activation of NF-kappaB and IKK requires an upstream kinase complex consisting of TAK1 and adaptor proteins such as TAB1, TAB2, or TAB3. TAK1 is in turn activated by TRAF6, a RING domain ubiquitin ligase that facilitates the synthesis of lysine 63-linked polyubiquitin chains. Here we present evidence that TAB2 and...

journal_title：Molecular cell

authors： Kanayama A,Seth RB,Sun L,Ea CK,Hong M,Shaito A,Chiu YH,Deng L,Chen ZJ

更新日期：2004-08-27 00:00:00

abstract：:It has been known for some time that stimulation of heterotrimeric G protein-coupled receptors can cause cytoskeletal reorganization. A recent report in Developmental Cell demonstrates that the activation of a tyrosine kinase, C-terminal Src kinase, by heterotrimeric G protein subunits provides the trigger for Rho-dep...

journal_title：Molecular cell

authors： Luttrell LM

更新日期：2002-06-01 00:00:00

abstract：:The crystal structure of a heterodimer between the ligand-binding domains (LBDs) of the human RARalpha bound to a selective antagonist and the constitutively active mouse RXRalphaF318A mutant shows that, pushed by a bulky extension of the ligand, RARalpha helix H12 adopts an antagonist position. The unexpected presenc...

journal_title：Molecular cell

authors： Bourguet W,Vivat V,Wurtz JM,Chambon P,Gronemeyer H,Moras D

更新日期：2000-02-01 00:00:00

abstract：:RNA-binding proteins coordinate the fates of multiple RNAs, but the principles underlying these global interactions remain poorly understood. We elucidated regulatory mechanisms of the RNA-binding protein HuR, by integrating data from diverse high-throughput targeting technologies, specifically PAR-CLIP, RIP-chip, and...

journal_title：Molecular cell

authors： Mukherjee N,Corcoran DL,Nusbaum JD,Reid DW,Georgiev S,Hafner M,Ascano M Jr,Tuschl T,Ohler U,Keene JD

更新日期：2011-08-05 00:00:00

abstract：:To exert regulatory function, miRNAs guide Argonaute (AGO) proteins to partially complementary sites on target RNAs. Crosslinking and immunoprecipitation (CLIP) assays are state-of-the-art to map AGO binding sites, but assigning the targeting miRNA to these sites relies on bioinformatics predictions and is therefore i...

journal_title：Molecular cell

authors： Grosswendt S,Filipchyk A,Manzano M,Klironomos F,Schilling M,Herzog M,Gottwein E,Rajewsky N

更新日期：2014-06-19 00:00:00

abstract：:Epigenetic indexing of chromatin domains by histone lysine methylation requires the balanced coordination of methyltransferase and demethylase activities. Here, we show that SU(VAR)3-3, the Drosophila homolog of the human LSD1 amine oxidase, demethylates H3K4me2 and H3K4me1 and facilitates subsequent H3K9 methylation ...

journal_title：Molecular cell

authors： Rudolph T,Yonezawa M,Lein S,Heidrich K,Kubicek S,Schäfer C,Phalke S,Walther M,Schmidt A,Jenuwein T,Reuter G

更新日期：2007-04-13 00:00:00

abstract：:HIV-1 Vpu interacts with CD4 in the endoplasmic reticulum and triggers CD4 degradation, presumably by proteasomes. Human beta TrCP identified by interaction with Vpu connects CD4 to this proteolytic machinery, and CD4-Vpu-beta TrCP ternary complexes have been detected by coimmunoprecipitation. beta TrCP binding to Vpu...

journal_title：Molecular cell

authors： Margottin F,Bour SP,Durand H,Selig L,Benichou S,Richard V,Thomas D,Strebel K,Benarous R

更新日期：1998-03-01 00:00:00

abstract：:The eukaryotic RNA polymerases Pol I, II, and III use different promoters to transcribe different classes of genes. Promoter usage relies on initiation factors, including TFIIF and TFIIE, in the case of Pol II. Here, we show that the Pol I-specific subunits A49 and A34.5 form a subcomplex that binds DNA and is related...

journal_title：Molecular cell

authors： Geiger SR,Lorenzen K,Schreieck A,Hanecker P,Kostrewa D,Heck AJ,Cramer P

更新日期：2010-08-27 00:00:00

abstract：:The papillomavirus major late protein, L1, forms the pentameric assembly unit of the viral shell. Recombinant HPV16 L1 pentamers assemble in vitro into capsid-like structures, and truncation of ten N-terminal residues leads to a homogeneous preparation of 12-pentamer, icosahedral particles. X-ray crystallographic anal...

journal_title：Molecular cell

authors： Chen XS,Garcea RL,Goldberg I,Casini G,Harrison SC

更新日期：2000-03-01 00:00:00

abstract：:The translocon is the gateway to the endoplasmic reticulum (ER). In yeast this is the Sec61p complex. However, new evidence suggests that a second translocon containing the Sec61p homolog Ssh1p provides important flexibility to the ER translocation machinery. ...

journal_title：Molecular cell

authors： Robb A,Brown JD

更新日期：2001-09-01 00:00:00

abstract：:Ubiquitin (UB)-driven signaling systems permeate biology, and are often integrated with other types of post-translational modifications (PTMs), including phosphorylation. Flux through such pathways is dictated by the fractional stoichiometry of distinct modifications and protein assemblies as well as the spatial organ...

journal_title：Molecular cell

authors： Ordureau A,Münch C,Harper JW

更新日期：2015-05-21 00:00:00

abstract：:Cell pattern in the ventral neural tube is organized by Sonic hedgehog (Shh) secreted by floor plate cells. To assay the range of direct Shh action, we developed a general method for blocking transduction of Hedgehog (Hh) signals through ectopic expression of a deleted form of the Hh receptor Patched (Ptc), termed Ptc...

journal_title：Molecular cell

authors： Briscoe J,Chen Y,Jessell TM,Struhl G

更新日期：2001-06-01 00:00:00

abstract：:Myosin VI is the only myosin that moves toward the minus end of actin filaments, suggesting a unique biological function. Here, we show that myosin VI is present in the nucleus of mammalian cells where it colocalizes with newly transcribed mRNA and with RNA polymerase II (RNAPII) and is detected in the RNAPII complex....

journal_title：Molecular cell

authors： Vreugde S,Ferrai C,Miluzio A,Hauben E,Marchisio PC,Crippa MP,Bussi M,Biffo S

更新日期：2006-09-01 00:00:00

abstract：:In this issue of Molecular Cell, detail molecular events that trigger apoptosis following growth factor withdrawal, linking irreversible mitochondrial permeabilization to activation of GSK-3 and subsequent phosphorylation, ubiquitinylation, and degradation of the antiapoptotic BCL-2 family member MCL-1. ...

journal_title：Molecular cell

authors： Letai A

更新日期：2006-03-17 00:00:00

abstract：:Cue1p is an integral component of yeast endoplasmic reticulum (ER)-associated degradation (ERAD) ubiquitin ligase (E3) complexes. It tethers the ERAD ubiquitin-conjugating enzyme (E2), Ubc7p, to the ER and prevents its degradation, and also activates Ubc7p via unknown mechanisms. We have now determined the crystal str...

journal_title：Molecular cell

authors： Metzger MB,Liang YH,Das R,Mariano J,Li S,Li J,Kostova Z,Byrd RA,Ji X,Weissman AM

更新日期：2013-05-23 00:00:00

abstract：:In this issue, Yoon et al. (2012) reveal that HuR influences decay of a long noncoding RNA (lincRNA-p21) that in turn hybridizes to mRNA targets and represses their translation-an indirect, but elegant explanation for the impact of HuR on translation efficiency. ...

journal_title：Molecular cell

authors： Wilusz CJ,Wilusz J

更新日期：2012-08-24 00:00:00

abstract：:Recent work from Kuehner and Brow (2008) and Thiebaut et al. (2008) in Molecular Cell and Jenks et al. (2008) in Molecular and Cellular Biology reveals that regulated expression of central nucleotide synthesis pathway components directs start site-dependent RNA polymerase II termination. ...

journal_title：Molecular cell

authors： Dichtl B

更新日期：2008-09-05 00:00:00

abstract：:Defective ER-resident membrane proteins need to be ejected into the cytoplasm in order to be degraded by the proteasome, but the exact mechanism remains unclear. In this issue of Molecular Cell, Neal et al. (2018) reveal that the rhomboid pseudoprotease Dfm1 defines the central ERAD component for membrane protein disl...

journal_title：Molecular cell

authors： Avci D,Lemberg MK

更新日期：2018-01-18 00:00:00

abstract：:Mismatch repair (MMR) is initiated by MutS family proteins (MSH) that recognize DNA mismatches and recruit downstream repair factors. We used a single-molecule DNA-unzipping assay to probe interactions between S. cerevisiae MSH2-MSH6 and a variety of DNA mismatch substrates. This work revealed a high-specificity bindi...

journal_title：Molecular cell

authors： Jiang J,Bai L,Surtees JA,Gemici Z,Wang MD,Alani E

更新日期：2005-12-09 00:00:00

abstract：:In bacterial and archaeal CRISPR immune pathways, DNA sequences from invading bacteriophage or plasmids are integrated into CRISPR loci within the host genome, conferring immunity against subsequent infections. The ribonucleoprotein complex Cascade utilizes RNAs generated from these loci to target complementary "nonse...

journal_title：Molecular cell

authors： Sashital DG,Wiedenheft B,Doudna JA

更新日期：2012-06-08 00:00:00

abstract：:Thousands of cis-elements in genomes are predicted to have vital functions. Although conservation, activity in surrogate assays, polymorphisms, and disease mutations provide functional clues, deletion from endogenous loci constitutes the gold-standard test. A GATA-2-binding, Gata2 intronic cis-element (+9.5) required ...

journal_title：Molecular cell

authors： Hewitt KJ,Kim DH,Devadas P,Prathibha R,Zuo C,Sanalkumar R,Johnson KD,Kang YA,Kim JS,Dewey CN,Keles S,Bresnick EH

更新日期：2015-07-02 00:00:00

abstract：:The proper processing, export, localization, translation, and degradation of mRNAs are necessary for regulation of gene expression. These processes are controlled by mRNA-specific regulatory proteins, noncoding RNAs, and core machineries common to most mRNAs. These factors bind the mRNA in large complexes known as mes...

journal_title：Molecular cell

authors： Mitchell SF,Parker R

更新日期：2014-05-22 00:00:00

abstract：:One fundamental function of telomeres is to prevent the ends of chromosomes from being sensed and treated as DNA damage. Here we present evidence for additional roles of telomeres in promoting proper chromosome segregation and DNA repair. We find that the fission yeast telomere protein Taz1p is required for cell cycle...

journal_title：Molecular cell

authors： Miller KM,Cooper JP

更新日期：2003-02-01 00:00:00

abstract：:Maintaining a fluid bilayer is essential for cell signaling and survival. Lipid saturation is a key factor determining lipid packing and membrane fluidity, and it must be tightly controlled to guarantee organelle function and identity. A dedicated eukaryotic mechanism of lipid saturation sensing, however, remains elus...

journal_title：Molecular cell

authors： Covino R,Ballweg S,Stordeur C,Michaelis JB,Puth K,Wernig F,Bahrami A,Ernst AM,Hummer G,Ernst R

更新日期：2016-07-07 00:00:00

abstract：:The carboxy-terminal domain (CTD) of the large subunit of RNA polymerase II (Pol II) comprises multiple heptapeptide repeats of the consensus Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Reversible phosphorylation of Ser2, Ser5, and Ser7 during the transcription cycle mediates the sequential recruitment of transcription/RNA pr...

journal_title：Molecular cell

authors： Egloff S,Zaborowska J,Laitem C,Kiss T,Murphy S

更新日期：2012-01-13 00:00:00

abstract：:Polymerase gamma, which replicates and repairs mitochondrial DNA, requires the Pol gamma B subunit for processivity. We determined the crystal structure of mouse Pol gamma B, a core component of the mitochondrial replication machinery. Pol gamma B shows high similarity to glycyl-tRNA synthetase and dimerizes through a...

journal_title：Molecular cell

authors： Carrodeguas JA,Theis K,Bogenhagen DF,Kisker C

更新日期：2001-01-01 00:00:00

abstract：:During meiosis, the homologous chromosomes pair and recombine. An evolutionarily conserved protein structure, the synaptonemal complex (SC), is located along the paired meiotic chromosomes. We have studied the function of a structural component in the axial/lateral element of the SC, the synaptonemal complex protein 3...

journal_title：Molecular cell

authors： Yuan L,Liu JG,Zhao J,Brundell E,Daneholt B,Höög C

更新日期：2000-01-01 00:00:00