A steroid-triggered transcriptional hierarchy controls salivary gland cell death during Drosophila metamorphosis.

abstract：:Cryo-EM structures of Dot1L in complex with a ubiquitinated nucleosome provide the long-sought-after molecular mechanism of Dot1L-mediated methylation of lysine 79 in histone H3 and explain crosstalk with histone H2B ubiquitination. ...

journal_title：Molecular cell

authors： Zhang Y,Kutateladze TG

更新日期：2019-06-06 00:00:00

abstract：:RING ubiquitin ligases (E3) recruit ubiquitin-conjugate enzymes (E2) charged with ubiquitin (Ub) to catalyze ubiquitination. Non-covalent Ub binding to the backside of certain E2s promotes processive polyUb formation, but the mechanism remains elusive. Here, we show that backside bound Ub (Ub(B)) enhances both RING-in...

journal_title：Molecular cell

authors： Buetow L,Gabrielsen M,Anthony NG,Dou H,Patel A,Aitkenhead H,Sibbet GJ,Smith BO,Huang DT

更新日期：2015-04-16 00:00:00

abstract：:RNA-binding proteins coordinate the fates of multiple RNAs, but the principles underlying these global interactions remain poorly understood. We elucidated regulatory mechanisms of the RNA-binding protein HuR, by integrating data from diverse high-throughput targeting technologies, specifically PAR-CLIP, RIP-chip, and...

journal_title：Molecular cell

authors： Mukherjee N,Corcoran DL,Nusbaum JD,Reid DW,Georgiev S,Hafner M,Ascano M Jr,Tuschl T,Ohler U,Keene JD

更新日期：2011-08-05 00:00:00

abstract：:Steroid hormones have rapid nongenomic effects on cell-signaling pathways, but the receptor mechanisms responsible for this are not understood. We have identified a specific polyproline motif in the amino-terminal domain of conventional progesterone receptor (PR) that mediates direct progestin-dependent interaction of...

journal_title：Molecular cell

authors： Boonyaratanakornkit V,Scott MP,Ribon V,Sherman L,Anderson SM,Maller JL,Miller WT,Edwards DP

更新日期：2001-08-01 00:00:00

abstract：:Trimethylation on H3K27 (H3K27me3) mediated by Polycomb repressive complex 2 (PRC2) has been linked to embryonic stem cell (ESC) identity and pluripotency. EZH2, the catalytic subunit of PRC2, has been reported as the sole histone methyltransferase that methylates H3K27 and mediates transcriptional silencing. Analysis...

journal_title：Molecular cell

authors： Shen X,Liu Y,Hsu YJ,Fujiwara Y,Kim J,Mao X,Yuan GC,Orkin SH

更新日期：2008-11-21 00:00:00

abstract：:Dysregulation of Wnt signaling has been implicated in tumorigenesis. The role of Transducin β-like proteins TBL1-TBLR1 in the promotion of Wnt/β-catenin-mediated oncogenesis has recently been emphasized; however, the molecular basis of activation of Wnt signaling by the corepressor TBL1-TBLR1 is incompletely understoo...

journal_title：Molecular cell

authors： Choi HK,Choi KC,Yoo JY,Song M,Ko SJ,Kim CH,Ahn JH,Chun KH,Yook JI,Yoon HG

更新日期：2011-07-22 00:00:00

abstract：:Recent studies, including two in this issue of Molecular Cell (Elbatsh et al., 2016; Beckouët et al., 2016), cast light on how cohesin regulators harness the energy of ATP hydrolysis to open the cohesin ring and enable dynamic, regulated entrapment of chromosomes. ...

journal_title：Molecular cell

authors： Yu H

更新日期：2016-02-18 00:00:00

abstract：:DNA replication introduces a dosage imbalance between early and late replicating genes. In budding yeast, buffering gene expression against this imbalance depends on marking replicated DNA by H3K56 acetylation (H3K56ac). Whether additional processes are required for suppressing transcription from H3K56ac-labeled DNA r...

journal_title：Molecular cell

authors： Voichek Y,Mittelman K,Gordon Y,Bar-Ziv R,Lifshitz Smit D,Shenhav R,Barkai N

更新日期：2018-06-21 00:00:00

abstract：:The PTEN tumor suppressor controls cell death and survival by regulating functions of various molecular targets. While the role of PTEN lipid-phosphatase activity on PtdIns(3,4,5)P3 and inhibition of PI3K pathway is well characterized, the biological relevance of PTEN protein-phosphatase activity remains undefined. He...

journal_title：Molecular cell

authors： Yip HYK,Chee A,Ang CS,Shin SY,Ooms LM,Mohammadi Z,Phillips WA,Daly RJ,Cole TJ,Bronson RT,Nguyen LK,Tiganis T,Hobbs RM,McLean CA,Mitchell CA,Papa A

更新日期：2020-10-15 00:00:00

abstract：:Poly-ubiquitin chains direct protein substrates to the 26S proteasome, where they are removed by the deubiquitinase Rpn11 during ATP-dependent substrate degradation. Rapid deubiquitination is required for efficient degradation but must be restricted to committed substrates that are engaged with the ATPase motor to pre...

journal_title：Molecular cell

authors： Worden EJ,Dong KC,Martin A

更新日期：2017-09-07 00:00:00

abstract：:Viral initiators perform multiple functions in initiation of DNA replication including ori binding, melting, and unwinding, culminating in the formation of a double hexameric (DH) helicase. We have recapitulated the assembly of the papillomavirus E1 initiator DH helicase, providing the first description of how such a ...

journal_title：Molecular cell

authors： Schuck S,Stenlund A

更新日期：2005-11-11 00:00:00

abstract：:Pre-mRNA splicing relies on the poorly understood dynamic interplay between >150 protein components of the spliceosome. The steps at which splicing can be regulated remain largely unknown. We systematically analyzed the effect of knocking down the components of the splicing machinery on alternative splicing events rel...

journal_title：Molecular cell

authors： Papasaikas P,Tejedor JR,Vigevani L,Valcárcel J

更新日期：2015-01-08 00:00:00

abstract：:The Drosophila gene vasa (vas) encodes an RNA-binding protein required for embryonic patterning and germ cell specification. In vas mutants, translation of several germline mRNAs is reduced. Here we show that VAS interacts directly with the Drosophila homolog of yeast translation initiation factor 2, encoded by a nove...

journal_title：Molecular cell

authors： Carrera P,Johnstone O,Nakamura A,Casanova J,Jäckle H,Lasko P

更新日期：2000-01-01 00:00:00

abstract：:Receptor-interacting protein kinase 3 (RIP3 or RIPK3) has emerged as a central player in necroptosis and a potential target to control inflammatory disease. Here, three selective small-molecule compounds are shown to inhibit RIP3 kinase-dependent necroptosis, although their therapeutic value is undermined by a surpris...

journal_title：Molecular cell

authors： Mandal P,Berger SB,Pillay S,Moriwaki K,Huang C,Guo H,Lich JD,Finger J,Kasparcova V,Votta B,Ouellette M,King BW,Wisnoski D,Lakdawala AS,DeMartino MP,Casillas LN,Haile PA,Sehon CA,Marquis RW,Upton J,Daley-Bauer LP,

更新日期：2014-11-20 00:00:00

abstract：:Ubiquitin modification of proteins plays pivotal roles in the cellular response to DNA damage. Given the complexity of ubiquitin conjugation due to the formation of poly-conjugates of different linkages, functional roles of linkage-specific ubiquitin modification at DNA damage sites are largely unclear. We identify th...

journal_title：Molecular cell

authors： Paul A,Wang B

更新日期：2017-05-18 00:00:00

abstract：:In this issue of Molecular Cell, Vonk et al. (2020) and Thiruvalluvan et al. (2020) identify key chaperones that confer resistance to protein aggregation in neural stem cells and become reduced upon differentiation. ...

journal_title：Molecular cell

authors： Molzahn CM,Mayor T

更新日期：2020-04-16 00:00:00

abstract：:Many genomic studies have revealed associations between the gut microbiota composition and host metabolism. These observations led to the idea that a causal relationship could exist between the microbiota and metabolic diseases, a concept supported by studies showing compositional changes in the microbial community in...

journal_title：Molecular cell

authors： Koh A,Bäckhed F

更新日期：2020-05-21 00:00:00

abstract：:In yeast Saccharomyces cerevisiae the G protein betagamma subunits (Ste4/Ste18) have long been known to transmit the signal required for mating. Here we demonstrate that GTPase-deficient mutants of Galpha (Gpa1) directly activate the mating response pathway. We also show that signaling by activated Gpa1 requires direc...

journal_title：Molecular cell

authors： Guo M,Aston C,Burchett SA,Dyke C,Fields S,Rajarao SJ,Uetz P,Wang Y,Young K,Dohlman HG

更新日期：2003-08-01 00:00:00

abstract：:Environmental cues provoke rapid transitions in gene expression to support growth and cellular plasticity through incompletely understood mechanisms. Lin28 RNA-binding proteins have evolutionarily conserved roles in post-transcriptional coordination of pro-growth gene expression, but signaling pathways allowing trophi...

journal_title：Molecular cell

authors： Amen AM,Ruiz-Garzon CR,Shi J,Subramanian M,Pham DL,Meffert MK

更新日期：2017-02-02 00:00:00

abstract：:The glycogen synthase kinase-3 (GSK3) family kinases are central cellular regulators highly conserved in all eukaryotes. In Arabidopsis, the GSK3-like kinase BIN2 phosphorylates a range of proteins to control broad developmental processes, and BIN2 is degraded through unknown mechanism upon receptor kinase-mediated br...

journal_title：Molecular cell

authors： Zhu JY,Li Y,Cao DM,Yang H,Oh E,Bi Y,Zhu S,Wang ZY

更新日期：2017-06-01 00:00:00

abstract：:Cells undergo apoptosis upon exposure to severe DNA damage stress. Under this condition, p73 is phosphorylated and activated by c-Abl. The transcription coactivator Yap1 binds p73 to generate a complex that escapes p73 proteasomal degradation and recruits p300 to support transcription of proapoptotic genes. However, t...

journal_title：Molecular cell

authors： Levy D,Adamovich Y,Reuven N,Shaul Y

更新日期：2008-02-15 00:00:00

abstract：:BIK protein is an initiator of mitochondrial apoptosis, and BIK expression is induced by proapoptotic signals, including DNA damage. Here, we demonstrate that 3' end processing and expression of BIK mRNA are controlled by the nuclear PI4,5P(2)-regulated poly(A) polymerase Star-PAP downstream of DNA damage. Nuclear PKC...

journal_title：Molecular cell

authors： Li W,Laishram RS,Ji Z,Barlow CA,Tian B,Anderson RA

更新日期：2012-01-13 00:00:00

abstract：:The ER-associated degradation (ERAD) pathway serves as an important cellular safeguard by directing incorrectly folded and unassembled proteins from the ER to the proteasome. Still, however, little is known about the components mediating ERAD of membrane proteins. Here we show that the evolutionary conserved rhomboid ...

journal_title：Molecular cell

authors： Fleig L,Bergbold N,Sahasrabudhe P,Geiger B,Kaltak L,Lemberg MK

更新日期：2012-08-24 00:00:00

abstract：:Metabolic reprogramming is a characteristic of cancer cells. Three studies published in this month's Molecular Cell provide novel insights into the role of mitochondrial pyruvate in tumor metabolism and describe how targeting pyruvate transport and metabolism may afford therapeutic benefit. ...

journal_title：Molecular cell

authors： Szlosarek PW,Lee S,Pollard PJ

更新日期：2014-11-06 00:00:00

abstract：:To map the genomic interaction sites of chromatin proteins, two related methods were developed and experimentally explored in Saccharomyces cerevisiae. The ChIC method (chromatin immunocleavage) consists of tethering a fusion protein (pA-MN) consisting of micrococcal nuclease (MN) and staphylococcal protein A to speci...

journal_title：Molecular cell

authors： Schmid M,Durussel T,Laemmli UK

更新日期：2004-10-08 00:00:00

abstract：:Signal-transduction cascades are usually studied on cell averages, masking variability between individual cells. To address this, we studied in individual cells the dynamic response of ERK2, a well-characterized MAPK signaling protein, which enters the nucleus upon stimulation. Using fluorescent tagging at the endogen...

journal_title：Molecular cell

authors： Cohen-Saidon C,Cohen AA,Sigal A,Liron Y,Alon U

更新日期：2009-12-11 00:00:00

abstract：:Replication forks frequently break and must be repaired by recombination. A reconstituted reaction now allows the factors that coordinate conversion from a recombination intermediate back to a replication fork to be defined. The PriA protein plays a key role in this control. ...

journal_title：Molecular cell

authors： Lovett ST

更新日期：2003-03-01 00:00:00

abstract：:Melanoma and other cancers harbor oncogenic mutations in the protein kinase B-Raf, which leads to constitutive activation and dysregulation of MAP kinase signaling. In order to elucidate molecular determinants responsible for B-Raf control of cancer phenotypes, we present a method for phosphoprotein profiling, using n...

journal_title：Molecular cell

authors： Old WM,Shabb JB,Houel S,Wang H,Couts KL,Yen CY,Litman ES,Croy CH,Meyer-Arendt K,Miranda JG,Brown RA,Witze ES,Schweppe RE,Resing KA,Ahn NG

更新日期：2009-04-10 00:00:00

abstract：:The formation of silenced and condensed heterochromatin foci involves enrichment of heterochromatin protein 1 (HP1). HP1 can bridge chromatin segments and form liquid droplets, but the biophysical principles underlying heterochromatin compartmentalization in the cell nucleus are elusive. Here, we assess mechanisticall...

journal_title：Molecular cell

authors： Erdel F,Rademacher A,Vlijm R,Tünnermann J,Frank L,Weinmann R,Schweigert E,Yserentant K,Hummert J,Bauer C,Schumacher S,Al Alwash A,Normand C,Herten DP,Engelhardt J,Rippe K

更新日期：2020-04-16 00:00:00

abstract：:We have identified human MBT domain-containing protein L3MBTL2 as an integral component of a protein complex that we termed Polycomb repressive complex 1 (PRC1)-like 4 (PRC1L4), given the copresence of PcG proteins RING1, RING2, and PCGF6/MBLR. PRC1L4 also contained E2F6 and CBX3/HP1γ, known to function in transcripti...

journal_title：Molecular cell

authors： Trojer P,Cao AR,Gao Z,Li Y,Zhang J,Xu X,Li G,Losson R,Erdjument-Bromage H,Tempst P,Farnham PJ,Reinberg D

更新日期：2011-05-20 00:00:00