Abstract：
:To determine whether replicational mutagenesis in the yeast genome is influenced by the positions of active origins, a reporter gene was placed in two orientations at multiple locations within a 39,000 bp region of chromosome III possessing two strong origins. The frequency of mutations resulting from misincorporation of adenine opposite 8-hydroxyguanine in one strand and 6-hydroxylaminopurine opposite cytosine in the other strand differed by 3- to 10-fold, depending on the gene orientation and its distance from the origins. The observed patterns indicate that active origins establish a strand bias for mutations that is maintained over thousands of base pairs and results from lower nucleotide selectivity and/or less efficient proofreading or mismatch repair during leading strand DNA replication.
journal_name
Mol Celljournal_title
Molecular cellauthors
Pavlov YI,Newlon CS,Kunkel TAdoi
10.1016/s1097-2765(02)00567-1subject
Has Abstractpub_date
2002-07-01 00:00:00pages
207-13issue
1eissn
1097-2765issn
1097-4164pii
S1097-2765(02)00567-1journal_volume
10pub_type
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