当前位置： SCI文献检索 > MOLECULAR CELL期刊下所有文献 > The dynamics of genome-wide DNA methylation reprogramming in mouse primordial germ cells.

The dynamics of genome-wide DNA methylation reprogramming in mouse primordial germ cells.

Abstract：

:Genome-wide DNA methylation reprogramming occurs in mouse primordial germ cells (PGCs) and preimplantation embryos, but the precise dynamics and biological outcomes are largely unknown. We have carried out whole-genome bisulfite sequencing (BS-Seq) and RNA-Seq across key stages from E6.5 epiblast to E16.5 PGCs. Global loss of methylation takes place during PGC expansion and migration with evidence for passive demethylation, but sequences that carry long-term epigenetic memory (imprints, CpG islands on the X chromosome, germline-specific genes) only become demethylated upon entry of PGCs into the gonads. The transcriptional profile of PGCs is tightly controlled despite global hypomethylation, with transient expression of the pluripotency network, suggesting that reprogramming and pluripotency are inextricably linked. Our results provide a framework for the understanding of the epigenetic ground state of pluripotency in the germline.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Seisenberger S,Andrews S,Krueger F,Arand J,Walter J,Santos F,Popp C,Thienpont B,Dean W,Reik W

doi

10.1016/j.molcel.2012.11.001

subject

Has Abstract

pub_date

2012-12-28 00:00:00

pages

849-62

issue

6

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(12)00932-X

journal_volume

48

pub_type

杂志文章

相关文献

MOLECULAR CELL文献大全
  • Coactivating factors p300 and CBP are transcriptionally crossregulated by Egr1 in prostate cells, leading to divergent responses.

    abstract：:Related coactivators p300 and CBP affect the transcriptional activities of many transcription factors (TF), producing multiple downstream effects. Here we show that immediate early response TF, Egr1, acts upstream of p300/CBP to induce or to repress transcription, depending on the stimulus. Cells induced with serum to...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2004.06.030

    authors： Yu J,de Belle I,Liang H,Adamson ED

    更新日期：2004-07-02 00:00:00

  • Molecular architecture of the human pre-mRNA 3' processing complex.

    abstract：:Pre-mRNA 3' end formation is an essential step in eukaryotic gene expression. Over half of human genes produce alternatively polyadenylated mRNAs, suggesting that regulated polyadenylation is an important mechanism for posttranscriptional gene control. Although a number of mammalian mRNA 3' processing factors have bee...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2008.12.028

    authors： Shi Y,Di Giammartino DC,Taylor D,Sarkeshik A,Rice WJ,Yates JR 3rd,Frank J,Manley JL

    更新日期：2009-02-13 00:00:00

  • Stopping the Clock with MYC.

    abstract：:In a recent paper in Cell Metabolism, Altman et al. (2015) report that MYC disrupts the molecular clock in cancer cells and describe a link between oncogenesis, circadian rhythms, and metabolism. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2015.11.008

    authors： Lau AN,Vander Heiden MG

    更新日期：2015-11-19 00:00:00

  • SOCS1 links cytokine signaling to p53 and senescence.

    abstract：:SOCS1 is lost in many human tumors, but its tumor suppression activities are not well understood. We report that SOCS1 is required for transcriptional activity, DNA binding, and serine 15 phosphorylation of p53 in the context of STAT5 signaling. In agreement, inactivation of SOCS1 disabled p53-dependent senescence in ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2009.09.044

    authors： Calabrese V,Mallette FA,Deschênes-Simard X,Ramanathan S,Gagnon J,Moores A,Ilangumaran S,Ferbeyre G

    更新日期：2009-12-11 00:00:00

  • Spindle assembly factor protection.

    abstract：:In this issue, Song et al. (2014) describe how microtubules and the RAN GTPase/importin-β system collaborate to control timing of HURP action and its turnover via the anaphase promoting complex (APC)-proteasome system, thereby ensuring temporal control of late mitotic events. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2014.01.007

    authors： Vaites LL,Harper JW

    更新日期：2014-01-23 00:00:00

  • HATs off to PIC assembly.

    abstract：:There are many regulated steps in the assembly of a transcription preinitiation complex (PIC). In this issue of Molecular Cell, Black et. al. (2006) reveal a catalytic switch mechanism in which autoacetylation of the HAT p300 triggers its dissociation from a promoter in a manner that is coupled to TFIID association. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章,评审

    doi：10.1016/j.molcel.2006.08.022

    authors： Pugh BF

    更新日期：2006-09-15 00:00:00

  • Regulation of SUMOylation by reversible oxidation of SUMO conjugating enzymes.

    abstract：:Posttranslational modification with small ubiquitin-related modifier (SUMO) has emerged as a central regulatory mechanism of protein function. However, little is known about the regulation of sumoylation itself. It has been reported that it is increased after exposure to various stresses including strong oxidative str...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2005.12.019

    authors： Bossis G,Melchior F

    更新日期：2006-02-03 00:00:00

  • The Biogenesis, Functions, and Challenges of Circular RNAs.

    abstract：:Covalently closed circular RNAs (circRNAs) are produced by precursor mRNA back-splicing of exons of thousands of genes in eukaryotes. circRNAs are generally expressed at low levels and often exhibit cell-type-specific and tissue-specific patterns. Recent studies have shown that their biogenesis requires spliceosomal m...

    journal_title：Molecular cell

    pub_type： 杂志文章,评审

    doi：10.1016/j.molcel.2018.06.034

    authors： Li X,Yang L,Chen LL

    更新日期：2018-08-02 00:00:00

  • Unambiguous identification of miRNA:target site interactions by different types of ligation reactions.

    abstract：:To exert regulatory function, miRNAs guide Argonaute (AGO) proteins to partially complementary sites on target RNAs. Crosslinking and immunoprecipitation (CLIP) assays are state-of-the-art to map AGO binding sites, but assigning the targeting miRNA to these sites relies on bioinformatics predictions and is therefore i...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2014.03.049

    authors： Grosswendt S,Filipchyk A,Manzano M,Klironomos F,Schilling M,Herzog M,Gottwein E,Rajewsky N

    更新日期：2014-06-19 00:00:00

  • A splicing-independent function of SF2/ASF in microRNA processing.

    abstract：:Both splicing factors and microRNAs are important regulatory molecules that play key roles in posttranscriptional gene regulation. By miRNA deep sequencing, we identified 40 miRNAs that are differentially expressed upon ectopic overexpression of the splicing factor SF2/ASF. Here we show that SF2/ASF and one of its upr...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2010.02.021

    authors： Wu H,Sun S,Tu K,Gao Y,Xie B,Krainer AR,Zhu J

    更新日期：2010-04-09 00:00:00

  • Replicational Dilution of H3K27me3 in Mammalian Cells and the Role of Poised Promoters.

    abstract：:Polycomb repressive complex 2 (PRC2) places H3K27me3 at developmental genes and is causally implicated in keeping bivalent genes silent. It is unclear if that silence requires minimum H3K27me3 levels and how the mark transmits faithfully across mammalian somatic cell generations. Mouse intestinal cells lacking EZH2 me...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2020.01.017

    authors： Jadhav U,Manieri E,Nalapareddy K,Madha S,Chakrabarti S,Wucherpfennig K,Barefoot M,Shivdasani RA

    更新日期：2020-04-02 00:00:00

  • Srs2 disassembles Rad51 filaments by a protein-protein interaction triggering ATP turnover and dissociation of Rad51 from DNA.

    abstract：:Rad51 is a DNA recombinase functioning in the repair of DNA double-strand breaks and the generation of genetic diversity by homologous recombination (HR). In the presence of ATP, Rad51 self-assembles into an extended polymer on single-stranded DNA to catalyze strand exchange. Inappropriate HR causes genomic instabilit...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2009.05.026

    authors： Antony E,Tomko EJ,Xiao Q,Krejci L,Lohman TM,Ellenberger T

    更新日期：2009-07-10 00:00:00

  • RIP3 induces apoptosis independent of pronecrotic kinase activity.

    abstract：:Receptor-interacting protein kinase 3 (RIP3 or RIPK3) has emerged as a central player in necroptosis and a potential target to control inflammatory disease. Here, three selective small-molecule compounds are shown to inhibit RIP3 kinase-dependent necroptosis, although their therapeutic value is undermined by a surpris...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2014.10.021

    authors： Mandal P,Berger SB,Pillay S,Moriwaki K,Huang C,Guo H,Lich JD,Finger J,Kasparcova V,Votta B,Ouellette M,King BW,Wisnoski D,Lakdawala AS,DeMartino MP,Casillas LN,Haile PA,Sehon CA,Marquis RW,Upton J,Daley-Bauer LP,

    更新日期：2014-11-20 00:00:00

  • General and versatile autoinhibition of PLC isozymes.

    abstract：:Phospholipase C (PLC) isozymes are directly activated by heterotrimeric G proteins and Ras-like GTPases to hydrolyze phosphatidylinositol 4,5-bisphosphate into the second messengers diacylglycerol and inositol 1,4,5-trisphosphate. Although PLCs play central roles in myriad signaling cascades, the molecular details of ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2008.06.018

    authors： Hicks SN,Jezyk MR,Gershburg S,Seifert JP,Harden TK,Sondek J

    更新日期：2008-08-08 00:00:00

  • Translation of CircRNAs.

    abstract：:Circular RNAs (circRNAs) are abundant and evolutionarily conserved RNAs of largely unknown function. Here, we show that a subset of circRNAs is translated in vivo. By performing ribosome footprinting from fly heads, we demonstrate that a group of circRNAs is associated with translating ribosomes. Many of these ribo-ci...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2017.02.021

    authors： Pamudurti NR,Bartok O,Jens M,Ashwal-Fluss R,Stottmeister C,Ruhe L,Hanan M,Wyler E,Perez-Hernandez D,Ramberger E,Shenzis S,Samson M,Dittmar G,Landthaler M,Chekulaeva M,Rajewsky N,Kadener S

    更新日期：2017-04-06 00:00:00

  • A small domain of CBP/p300 binds diverse proteins: solution structure and functional studies.

    abstract：:The transcriptional coactivators CBP and p300 are critical regulators of metazoan gene expression. They associate with many different DNA-bound transcription factors through small, conserved domains. We have identified a compactly folded 46 residue domain in CBP and p300, the IRF-3 binding domain (IBiD), and we have d...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(01)00333-1

    authors： Lin CH,Hare BJ,Wagner G,Harrison SC,Maniatis T,Fraenkel E

    更新日期：2001-09-01 00:00:00

  • Epigenetic Control of Expression Homeostasis during Replication Is Stabilized by the Replication Checkpoint.

    abstract：:DNA replication introduces a dosage imbalance between early and late replicating genes. In budding yeast, buffering gene expression against this imbalance depends on marking replicated DNA by H3K56 acetylation (H3K56ac). Whether additional processes are required for suppressing transcription from H3K56ac-labeled DNA r...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2018.05.015

    authors： Voichek Y,Mittelman K,Gordon Y,Bar-Ziv R,Lifshitz Smit D,Shenhav R,Barkai N

    更新日期：2018-06-21 00:00:00

  • The Promise of Proteomics for the Study of ADP-Ribosylation.

    abstract：:ADP-ribosylation is a post-translational modification where single units (mono-ADP-ribosylation) or polymeric chains (poly-ADP-ribosylation) of ADP-ribose are conjugated to proteins by ADP-ribosyltransferases. This post-translational modification and the ADP-ribosyltransferases (also known as PARPs) responsible for it...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2015.06.012

    authors： Daniels CM,Ong SE,Leung AK

    更新日期：2015-06-18 00:00:00

  • The evolutionarily conserved core design of the catalytic activation step of the yeast spliceosome.

    abstract：:Metazoan spliceosomes exhibit an elaborate protein composition required for canonical and alternative splicing. Thus, the minimal set of proteins essential for activation and catalysis remains elusive. We therefore purified in vitro assembled, precatalytic spliceosomal complex B, activated B(act), and step 1 complex C...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2009.09.040

    authors： Fabrizio P,Dannenberg J,Dube P,Kastner B,Stark H,Urlaub H,Lührmann R

    更新日期：2009-11-25 00:00:00

  • EGFR-Phosphorylated Platelet Isoform of Phosphofructokinase 1 Promotes PI3K Activation.

    abstract：:EGFR activates phosphatidylinositide 3-kinase (PI3K), but the mechanism underlying this activation is not completely understood. We demonstrated here that EGFR activation resulted in lysine acetyltransferase 5 (KAT5)-mediated K395 acetylation of the platelet isoform of phosphofructokinase 1 (PFKP) and subsequent trans...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2018.03.018

    authors： Lee JH,Liu R,Li J,Wang Y,Tan L,Li XJ,Qian X,Zhang C,Xia Y,Xu D,Guo W,Ding Z,Du L,Zheng Y,Chen Q,Lorenzi PL,Mills GB,Jiang T,Lu Z

    更新日期：2018-04-19 00:00:00

  • MicroRNA pathways modulate polyglutamine-induced neurodegeneration.

    abstract：:Nine human neurodegenerative diseases are due to expansion of a CAG repeat- encoding glutamine within the open reading frame of the respective genes. Polyglutamine (polyQ) expansion confers dominant toxicity, resulting in neuronal degeneration. MicroRNAs (miRNAs) have been shown to modulate programmed cell death durin...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2006.07.030

    authors： Bilen J,Liu N,Burnett BG,Pittman RN,Bonini NM

    更新日期：2006-10-06 00:00:00

  • Pin1At encoding a peptidyl-prolyl cis/trans isomerase regulates flowering time in Arabidopsis.

    abstract：:Floral transition in plants is regulated by an integrated network of flowering genetic pathways. We show that an Arabidopsis PIN1-type parvulin 1, Pin1At, controls floral transition by accelerating cis/trans isomerization of the phosphorylated Ser/Thr-Pro motifs in two MADS-domain transcription factors, SUPPRESSOR OF ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2009.12.020

    authors： Wang Y,Liu C,Yang D,Yu H,Liou YC

    更新日期：2010-01-15 00:00:00

  • The K48-K63 Branched Ubiquitin Chain Regulates NF-κB Signaling.

    abstract：:Polyubiquitin chains of different topologies regulate diverse cellular processes. K48- and K63-linked chains, the two most abundant chain types, regulate proteolytic and signaling pathways, respectively. Although recent studies reported important roles for heterogeneous chains, the functions of branched ubiquitin chai...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2016.09.014

    authors： Ohtake F,Saeki Y,Ishido S,Kanno J,Tanaka K

    更新日期：2016-10-20 00:00:00

  • To Cut or Not to Cut: Discovery of a Novel Regulator of DNA Break Resection.

    abstract：:Nucleolytic resection of DNA double-strand breaks is the crucial first step in their repair via homologous recombination. New findings by Tkáč et al. (2016) published in this issue of Molecular Cell identify HELB as a novel, cell-cycle-specific negative regulator of DNA end resection. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2016.01.022

    authors： Daley JM,Sung P

    更新日期：2016-02-04 00:00:00

  • Structure of a Cytoplasmic 11-Subunit RNA Exosome Complex.

    abstract：:The RNA exosome complex associates with nuclear and cytoplasmic cofactors to mediate the decay, surveillance, or processing of a wide variety of transcripts. In the cytoplasm, the conserved core of the exosome (Exo10) functions together with the conserved Ski complex. The interaction of S. cerevisiae Exo10 and Ski is ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2016.05.028

    authors： Kowalinski E,Kögel A,Ebert J,Reichelt P,Stegmann E,Habermann B,Conti E

    更新日期：2016-07-07 00:00:00

  • Insights into degron recognition by APC/C coactivators from the structure of an Acm1-Cdh1 complex.

    abstract：:The anaphase-promoting complex/cyclosome (APC/C) regulates sister chromatid segregation and the exit from mitosis. Selection of most APC/C substrates is controlled by coactivator subunits (either Cdc20 or Cdh1) that interact with substrate destruction motifs--predominantly the destruction (D) box and KEN box degrons. ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2013.04.024

    authors： He J,Chao WC,Zhang Z,Yang J,Cronin N,Barford D

    更新日期：2013-06-06 00:00:00

  • Rhythmic PER abundance defines a critical nodal point for negative feedback within the circadian clock mechanism.

    abstract：:Circadian rhythms in mammals are generated by a transcriptional negative feedback loop that is driven primarily by oscillations of PER and CRY, which inhibit their own transcriptional activators, CLOCK and BMAL1. Current models posit that CRY is the dominant repressor, while PER may play an accessory role. In this stu...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2009.10.012

    authors： Chen R,Schirmer A,Lee Y,Lee H,Kumar V,Yoo SH,Takahashi JS,Lee C

    更新日期：2009-11-13 00:00:00

  • PPL2 translesion polymerase is essential for the completion of chromosomal DNA replication in the African trypanosome.

    abstract：:Faithful copying of the genome is essential for life. In eukaryotes, a single archaeo-eukaryotic primase (AEP), DNA primase, is required for the initiation and progression of DNA replication. Here we have identified additional eukaryotic AEP-like proteins with DNA-dependent primase and/or polymerase activity. Uniquely...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2013.10.034

    authors： Rudd SG,Glover L,Jozwiakowski SK,Horn D,Doherty AJ

    更新日期：2013-11-21 00:00:00

  • Proteasome active sites allosterically regulate each other, suggesting a cyclical bite-chew mechanism for protein breakdown.

    abstract：:In eukaryotes, the 20S proteasome contains two chymotrypsin-like, two trypsin-like, and two active sites shown here to have caspase-like specificity. We report that certain sites allosterically regulate each other's activities. Substrates of a chymotrypsin-like site stimulate dramatically the caspase-like activity and...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(00)80341-x

    authors： Kisselev AF,Akopian TN,Castillo V,Goldberg AL

    更新日期：1999-09-01 00:00:00

  • Activation and inhibition of the receptor histidine kinase AgrC occurs through opposite helical transduction motions.

    abstract：:Staphylococcus aureus virulence is regulated when secreted autoinducing peptides (AIPs) are recognized by a membrane-bound receptor histidine kinase (RHK), AgrC. Some AIPs are agonists of virulence gene expression, while others are antagonists. It is unclear how AIP binding regulates AgrC activity. Here, we reconstitu...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2014.02.029

    authors： Wang B,Zhao A,Novick RP,Muir TW

    更新日期：2014-03-20 00:00:00