当前位置： SCI文献检索 > MOLECULAR CELL期刊下所有文献 > Molecular architecture of the human pre-mRNA 3' processing complex.

Molecular architecture of the human pre-mRNA 3' processing complex.

Abstract：

:Pre-mRNA 3' end formation is an essential step in eukaryotic gene expression. Over half of human genes produce alternatively polyadenylated mRNAs, suggesting that regulated polyadenylation is an important mechanism for posttranscriptional gene control. Although a number of mammalian mRNA 3' processing factors have been identified, the full protein composition of the 3' processing machinery has not been determined, and its structure is unknown. Here we report the purification and subsequent proteomic and structural characterization of human mRNA 3' processing complexes. Remarkably, the purified 3' processing complex contains approximately 85 proteins, including known and new core 3' processing factors and over 50 proteins that may mediate crosstalk with other processes. Electron microscopic analyses show that the core 3' processing complex has a distinct "kidney" shape and is approximately 250 A in length. Together, our data has revealed the complexity and molecular architecture of the pre-mRNA 3' processing complex.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Shi Y,Di Giammartino DC,Taylor D,Sarkeshik A,Rice WJ,Yates JR 3rd,Frank J,Manley JL

doi

10.1016/j.molcel.2008.12.028

subject

Has Abstract

pub_date

2009-02-13 00:00:00

pages

365-76

issue

3

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(09)00025-2

journal_volume

33

pub_type

杂志文章

相关文献

MOLECULAR CELL文献大全
  • Regulation of the histone H4 monomethylase PR-Set7 by CRL4(Cdt2)-mediated PCNA-dependent degradation during DNA damage.

    abstract：:The histone methyltransferase PR-Set7/Set8 is the sole enzyme that catalyzes monomethylation of histone H4 at K20 (H4K20me1). Previous reports document disparate evidence regarding PR-Set7 expression during the cell cycle, the biological relevance of PR-Set7 interaction with PCNA, and its role in the cell. We find tha...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2010.10.011

    authors： Oda H,Hübner MR,Beck DB,Vermeulen M,Hurwitz J,Spector DL,Reinberg D

    更新日期：2010-11-12 00:00:00

  • Topoisomerase II inactivation prevents the completion of DNA replication in budding yeast.

    abstract：:Type II topoisomerases are essential for resolving topologically entwined double-stranded DNA. Although anti-topoisomerase 2 (Top2) drugs are clinically important antibiotics and chemotherapies, to our knowledge, the mechanisms of cell killing by Top2 depletion and inactivation have never been directly compared. We sh...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2008.04.019

    authors： Baxter J,Diffley JF

    更新日期：2008-06-20 00:00:00

  • An Arginine Nexus in the RNA Polymerase II CTD.

    abstract：:In this issue of Molecular Cell,Sharma et al. (2019) show that normal cell growth requires conversion of an arginine residue in the RNA polymerase II C-terminal domain (CTD) to citrulline, uncovering a potential regulatory pathway involving opposing arginine modifications. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2018.12.013

    authors： Corden JL

    更新日期：2019-01-03 00:00:00

  • BRCA1 recruitment to transcriptional pause sites is required for R-loop-driven DNA damage repair.

    abstract：:The mechanisms contributing to transcription-associated genomic instability are both complex and incompletely understood. Although R-loops are normal transcriptional intermediates, they are also associated with genomic instability. Here, we show that BRCA1 is recruited to R-loops that form normally over a subset of tr...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2015.01.011

    authors： Hatchi E,Skourti-Stathaki K,Ventz S,Pinello L,Yen A,Kamieniarz-Gdula K,Dimitrov S,Pathania S,McKinney KM,Eaton ML,Kellis M,Hill SJ,Parmigiani G,Proudfoot NJ,Livingston DM

    更新日期：2015-02-19 00:00:00

  • Single-cell transcriptomics enters the age of mass production.

    abstract：:Two publications in the current issue of Cell introduce novel methods for high-throughput single-cell transcriptomics by using droplet microfluidics and sophisticated barcoding schemes for transcriptional profiling of thousands of individual cells. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2015.05.019

    authors： Junker JP,van Oudenaarden A

    更新日期：2015-05-21 00:00:00

  • Multidomain Convergence of Argonaute during RISC Assembly Correlates with the Formation of Internal Water Clusters.

    abstract：:Despite the relevance of Argonaute proteins in RNA silencing, little is known about the structural steps of small RNA loading to form RNA-induced silencing complexes (RISCs). We report the 1.9 Å crystal structure of human Argonaute4 with guide RNA. Comparison with the previously determined apo structure of Neurospora ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2019.06.011

    authors： Park MS,Araya-Secchi R,Brackbill JA,Phan HD,Kehling AC,Abd El-Wahab EW,Dayeh DM,Sotomayor M,Nakanishi K

    更新日期：2019-08-22 00:00:00

  • Mitochondrial disulfide bond formation is driven by intersubunit electron transfer in Erv1 and proofread by glutathione.

    abstract：:The disulfide relay system in the intermembrane space of mitochondria is of crucial importance for mitochondrial biogenesis. Major players in this pathway are the oxidoreductase Mia40 that oxidizes substrates and the sulfhydryl oxidase Erv1 that reoxidizes Mia40. To analyze in detail the mechanism of this oxidative pa...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2010.01.017

    authors： Bien M,Longen S,Wagener N,Chwalla I,Herrmann JM,Riemer J

    更新日期：2010-02-26 00:00:00

  • Methylation of DNA Ligase 1 by G9a/GLP Recruits UHRF1 to Replicating DNA and Regulates DNA Methylation.

    abstract：:DNA methylation is an essential epigenetic mark in mammals that has to be re-established after each round of DNA replication. The protein UHRF1 is essential for this process; it has been proposed that the protein targets newly replicated DNA by cooperatively binding hemi-methylated DNA and H3K9me2/3, but this model le...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2017.07.012

    authors： Ferry L,Fournier A,Tsusaka T,Adelmant G,Shimazu T,Matano S,Kirsh O,Amouroux R,Dohmae N,Suzuki T,Filion GJ,Deng W,de Dieuleveult M,Fritsch L,Kudithipudi S,Jeltsch A,Leonhardt H,Hajkova P,Marto JA,Arita K,Shinkai Y

    更新日期：2017-08-17 00:00:00

  • miR-182-mediated downregulation of BRCA1 impacts DNA repair and sensitivity to PARP inhibitors.

    abstract：:Expression of BRCA1 is commonly decreased in sporadic breast tumors, and this correlates with poor prognosis of breast cancer patients. Here we show that BRCA1 transcripts are selectively enriched in the Argonaute/miR-182 complex and miR-182 downregulates BRCA1 expression. Antagonizing miR-182 enhances BRCA1 protein l...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2010.12.005

    authors： Moskwa P,Buffa FM,Pan Y,Panchakshari R,Gottipati P,Muschel RJ,Beech J,Kulshrestha R,Abdelmohsen K,Weinstock DM,Gorospe M,Harris AL,Helleday T,Chowdhury D

    更新日期：2011-01-21 00:00:00

  • R-Loop Mediated trans Action of the APOLO Long Noncoding RNA.

    abstract：:In eukaryotes, three-dimensional genome organization is critical for transcriptional regulation of gene expression. Long noncoding RNAs (lncRNAs) can modulate chromatin conformation of spatially related genomic locations within the nucleus. Here, we show that the lncRNA APOLO (AUXIN-REGULATED PROMOTER LOOP) recognizes...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2019.12.015

    authors： Ariel F,Lucero L,Christ A,Mammarella MF,Jegu T,Veluchamy A,Mariappan K,Latrasse D,Blein T,Liu C,Benhamed M,Crespi M

    更新日期：2020-03-05 00:00:00

  • The little elongation complex functions at initiation and elongation phases of snRNA gene transcription.

    abstract：:The small nuclear RNA (snRNA) genes have been widely used as a model system for understanding transcriptional regulation due to the unique aspects of their promoter structure, selectivity for either RNA polymerase (Pol) II or III, and because of their unique mechanism of termination that is tightly linked with the pro...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2013.07.003

    authors： Hu D,Smith ER,Garruss AS,Mohaghegh N,Varberg JM,Lin C,Jackson J,Gao X,Saraf A,Florens L,Washburn MP,Eissenberg JC,Shilatifard A

    更新日期：2013-08-22 00:00:00

  • Chromatin disassembly mediated by the histone chaperone Asf1 is essential for transcriptional activation of the yeast PHO5 and PHO8 genes.

    abstract：:Nucleosome loss from a promoter region has recently been described as a potential mechanism for transcriptional regulation. We investigated whether H3/H4 histone chaperones mediate the loss of nucleosomes from the promoter of the yeast PHO5 gene during transcriptional activation. We found that antisilencing function 1...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2004.05.016

    authors： Adkins MW,Howar SR,Tyler JK

    更新日期：2004-06-04 00:00:00

  • Linking activators and basals in transcription: it is all in one family.

    abstract：:Analysis of nuclear hormone receptor function in cells derived from xeroderma pigmentosum patients reveals novel links between DNA repair and transcription and points to novel mechanisms of regulating transcriptional regulators. ...

    journal_title：Molecular cell

    pub_type： 杂志文章,评审

    doi：10.1016/s1097-2765(02)00511-7

    authors： Timmers HT

    更新日期：2002-04-01 00:00:00

  • MLKL Requires the Inositol Phosphate Code to Execute Necroptosis.

    abstract：:Necroptosis is an important form of lytic cell death triggered by injury and infection, but whether mixed lineage kinase domain-like (MLKL) is sufficient to execute this pathway is unknown. In a genetic selection for human cell mutants defective for MLKL-dependent necroptosis, we identified mutations in IPMK and ITPK1...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2018.05.010

    authors： Dovey CM,Diep J,Clarke BP,Hale AT,McNamara DE,Guo H,Brown NW Jr,Cao JY,Grace CR,Gough PJ,Bertin J,Dixon SJ,Fiedler D,Mocarski ES,Kaiser WJ,Moldoveanu T,York JD,Carette JE

    更新日期：2018-06-07 00:00:00

  • Prolyl cis-trans Isomerization as a molecular timer in Crk signaling.

    abstract：:In a recent issue of Molecular Cell, use NMR to identify peptidyl-prolyl cis-trans isomerization as an autoinhibitory switch in the adaptor protein Crk, suggesting a new mechanism for controlling the rate of formation of signaling complexes. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2007.02.005

    authors： Nicholson LK,Lu KP

    更新日期：2007-02-23 00:00:00

  • Hsp90-dependent activation of protein kinases is regulated by chaperone-targeted dephosphorylation of Cdc37.

    abstract：:Activation of protein kinase clients by the Hsp90 system is mediated by the cochaperone protein Cdc37. Cdc37 requires phosphorylation at Ser13, but little is known about the regulation of this essential posttranslational modification. We show that Ser13 of uncomplexed Cdc37 is phosphorylated in vivo, as well as in bin...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2008.07.021

    authors： Vaughan CK,Mollapour M,Smith JR,Truman A,Hu B,Good VM,Panaretou B,Neckers L,Clarke PA,Workman P,Piper PW,Prodromou C,Pearl LH

    更新日期：2008-09-26 00:00:00

  • Formation of a novel four-helix bundle and molecular recognition sites by dimerization of a response regulator phosphotransferase.

    abstract：:A basis for understanding specificity of molecular recognition between phosphorelay proteins has been deduced from the 2.6 A structure of the Spo0B phosphotransferase of the phosphorelay regulating sporulation initiation. Spo0B consists of two domains: an N-terminal alpha-helical hairpin domain and a C-terminal alpha/...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(00)80148-3

    authors： Varughese KI,Madhusudan,Zhou XZ,Whiteley JM,Hoch JA

    更新日期：1998-10-01 00:00:00

  • The F-box Protein KIB1 Mediates Brassinosteroid-Induced Inactivation and Degradation of GSK3-like Kinases in Arabidopsis.

    abstract：:The glycogen synthase kinase-3 (GSK3) family kinases are central cellular regulators highly conserved in all eukaryotes. In Arabidopsis, the GSK3-like kinase BIN2 phosphorylates a range of proteins to control broad developmental processes, and BIN2 is degraded through unknown mechanism upon receptor kinase-mediated br...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2017.05.012

    authors： Zhu JY,Li Y,Cao DM,Yang H,Oh E,Bi Y,Zhu S,Wang ZY

    更新日期：2017-06-01 00:00:00

  • The plug domain of the SecY protein stabilizes the closed state of the translocation channel and maintains a membrane seal.

    abstract：:Proteins are translocated across membranes through a channel that is formed by the prokaryotic SecY or eukaryotic Sec61 complex. The crystal structure of the SecY channel from M. jannaschii revealed a plug domain that appears to seal the channel in its closed state. However, the role of the plug remains unclear, parti...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2007.05.002

    authors： Li W,Schulman S,Boyd D,Erlandson K,Beckwith J,Rapoport TA

    更新日期：2007-05-25 00:00:00

  • Ribosome components are associated with sites of transcription.

    abstract：:It is generally believed that eukaryotic ribosomes first associate with mRNA in the cytoplasm. However, we show with chromosomal immunostaining and in situ hybridization that ribosomal subunits are present at transcription sites of Drosophila salivary gland chromosomes. Immunostaining was carried out with antibodies s...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：

    authors： Brogna S,Sato TA,Rosbash M

    更新日期：2002-07-01 00:00:00

  • The crystal structure and mode of action of trans-sialidase, a key enzyme in Trypanosoma cruzi pathogenesis.

    abstract：:Trans-sialidases (TS) are GPI-anchored surface enzymes expressed in specific developmental stages of trypanosome parasites like Trypanosoma cruzi, the etiologic agent of Chagas disease, and T. brucei, the causative agent of sleeping sickness. TS catalyzes the transfer of sialic acid residues from host to parasite glyc...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(02)00680-9

    authors： Buschiazzo A,Amaya MF,Cremona ML,Frasch AC,Alzari PM

    更新日期：2002-10-01 00:00:00

  • Modular RNA heats up.

    abstract：:In this issue of Molecular Cell, Mariner et al. (2008) demonstrate that Alu RNA from a human SINE represses RNA polymerase II transcription during heat shock. This noncoding RNA is the first example of a "protein-like" transcription factor with a distinct modular architecture. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2008.02.001

    authors： Shamovsky I,Nudler E

    更新日期：2008-02-29 00:00:00

  • The Chaperone Activity of the Developmental Small Heat Shock Protein Sip1 Is Regulated by pH-Dependent Conformational Changes.

    abstract：:Small heat shock proteins (sHsps) are ubiquitous molecular chaperones that prevent the aggregation of unfolding proteins during proteotoxic stress. In Caenorhabditis elegans, Sip1 is the only sHsp exclusively expressed in oocytes and embryos. Here, we demonstrate that Sip1 is essential for heat shock survival of repro...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2015.04.019

    authors： Fleckenstein T,Kastenmüller A,Stein ML,Peters C,Daake M,Krause M,Weinfurtner D,Haslbeck M,Weinkauf S,Groll M,Buchner J

    更新日期：2015-06-18 00:00:00

  • Phosphatidic Acid Signals via the Hippo Pathway.

    abstract：:In this issue of Molecular Cell, Han et al. (2018) show that phosphatidic acid inhibits the action of the LATS kinases, key mediators of Hippo pathway signaling, which leads to an increase in the nuclear activity of the transcriptional regulator YAP. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2018.10.004

    authors： Stampouloglou E,Varelas X

    更新日期：2018-10-18 00:00:00

  • JunD protects cells from p53-dependent senescence and apoptosis.

    abstract：:JunD is the most broadly expressed member of the Jun family and the AP-1 transcription factor complex. Primary fibroblasts lacking JunD displayed p53-dependent growth arrest, upregulated p19(Arf) expression, and premature senescence. In contrast, immortalized cell lines lacking JunD showed increased proliferation and ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(00)00109-x

    authors： Weitzman JB,Fiette L,Matsuo K,Yaniv M

    更新日期：2000-11-01 00:00:00

  • A robust toolkit for functional profiling of the yeast genome.

    abstract：:Study of mutant phenotypes is a fundamental method for understanding gene function. The construction of a near-complete collection of yeast knockouts (YKO) and the unique molecular barcodes (or TAGs) that identify each strain has enabled quantitative functional profiling of Saccharomyces cerevisiae. By using these TAG...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2004.09.035

    authors： Pan X,Yuan DS,Xiang D,Wang X,Sookhai-Mahadeo S,Bader JS,Hieter P,Spencer F,Boeke JD

    更新日期：2004-11-05 00:00:00

  • Spatial and temporal recruitment of androgen receptor and its coactivators involves chromosomal looping and polymerase tracking.

    abstract：:Androgen receptor (AR) plays a critical role in the development and progression of prostate cancer, where it is a key therapeutic target. Here we report that, in contrast to estrogen receptor transcription complexes which form within minutes and recycle hourly, the levels of regulatory regions bound by AR complexes ri...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2005.07.018

    authors： Wang Q,Carroll JS,Brown M

    更新日期：2005-09-02 00:00:00

  • Proteasome active sites allosterically regulate each other, suggesting a cyclical bite-chew mechanism for protein breakdown.

    abstract：:In eukaryotes, the 20S proteasome contains two chymotrypsin-like, two trypsin-like, and two active sites shown here to have caspase-like specificity. We report that certain sites allosterically regulate each other's activities. Substrates of a chymotrypsin-like site stimulate dramatically the caspase-like activity and...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(00)80341-x

    authors： Kisselev AF,Akopian TN,Castillo V,Goldberg AL

    更新日期：1999-09-01 00:00:00

  • Stay close to your sister.

    abstract：:Yeast Sir2 protein regulates chromatin structure and suppresses recombination at the multiple tandem rDNA array. In the current issue of Cell, Kobayashi and colleagues reinvestigate rDNA dynamics in sir2 strains and find that Sir2 is necessary to recruit cohesins to the array, which influences the nature of rDNA recom...

    journal_title：Molecular cell

    pub_type： 杂志文章,评审

    doi：10.1016/s1097-2765(04)00266-7

    authors： Reid RJ,Rothstein R

    更新日期：2004-05-21 00:00:00

  • E3-Independent Constitutive Monoubiquitination Complements Histone Methyltransferase Activity of SETDB1.

    abstract：:Ubiquitination typically occurs through the sequential action of three enzymes catalyzing ubiquitin activation (E1), conjugation (E2), and ligation (E3) and regulates diverse eukaryotic cellular processes. Although monoubiquitination commonly confers nondegradative activities, mechanisms underlying its temporal and sp...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2016.04.022

    authors： Sun L,Fang J

    更新日期：2016-06-16 00:00:00