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The crystal structure and mode of action of trans-sialidase, a key enzyme in Trypanosoma cruzi pathogenesis.

Abstract：

:Trans-sialidases (TS) are GPI-anchored surface enzymes expressed in specific developmental stages of trypanosome parasites like Trypanosoma cruzi, the etiologic agent of Chagas disease, and T. brucei, the causative agent of sleeping sickness. TS catalyzes the transfer of sialic acid residues from host to parasite glycoconjugates through a transglycosidase reaction that appears to be critical for T. cruzi survival and cell invasion capability. We report here the structure of the T. cruzi trans-sialidase, alone and in complex with sugar ligands. Sialic acid binding is shown to trigger a conformational switch that modulates the affinity for the acceptor substrate and concomitantly creates the conditions for efficient transglycosylation. The structure provides a framework for the structure-based design of novel inhibitors with potential therapeutic applications.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Buschiazzo A,Amaya MF,Cremona ML,Frasch AC,Alzari PM

doi

10.1016/s1097-2765(02)00680-9

subject

Has Abstract

pub_date

2002-10-01 00:00:00

pages

757-68

issue

4

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(02)00680-9

journal_volume

10

pub_type

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