当前位置： SCI文献检索 > MOLECULAR CELL期刊下所有文献 > PPL2 translesion polymerase is essential for the completion of chromosomal DNA replication in the African trypanosome.

PPL2 translesion polymerase is essential for the completion of chromosomal DNA replication in the African trypanosome.

Abstract：

:Faithful copying of the genome is essential for life. In eukaryotes, a single archaeo-eukaryotic primase (AEP), DNA primase, is required for the initiation and progression of DNA replication. Here we have identified additional eukaryotic AEP-like proteins with DNA-dependent primase and/or polymerase activity. Uniquely, the genomes of trypanosomatids, a group of kinetoplastid protozoa of significant medical importance, encode two PrimPol-like (PPL) proteins. In the African trypanosome, PPL2 is a nuclear enzyme present in G2 phase cells. Following PPL2 knockdown, a cell-cycle arrest occurs after the bulk of DNA synthesis, the DNA damage response is activated, and cells fail to recover. Consistent with this phenotype, PPL2 replicates damaged DNA templates in vitro, including templates containing the UV-induced pyrimidine-pyrimidone (6-4) photoproduct. Furthermore, PPL2 accumulates at sites of nuclear DNA damage. Taken together, our results indicate an essential role for PPL2 in postreplication tolerance of endogenous DNA damage, thus allowing completion of genome duplication.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Rudd SG,Glover L,Jozwiakowski SK,Horn D,Doherty AJ

doi

10.1016/j.molcel.2013.10.034

subject

Has Abstract

pub_date

2013-11-21 00:00:00

pages

554-65

issue

4

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(13)00800-9

journal_volume

52

pub_type

杂志文章

相关文献

MOLECULAR CELL文献大全
  • PoxA, yjeK, and elongation factor P coordinately modulate virulence and drug resistance in Salmonella enterica.

    abstract：:We report an interaction between poxA, encoding a paralog of lysyl tRNA-synthetase, and the closely linked yjeK gene, encoding a putative 2,3-beta-lysine aminomutase, that is critical for virulence and stress resistance in Salmonella enterica. Salmonella poxA and yjeK mutants share extensive phenotypic pleiotropy, inc...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2010.06.021

    authors： Navarre WW,Zou SB,Roy H,Xie JL,Savchenko A,Singer A,Edvokimova E,Prost LR,Kumar R,Ibba M,Fang FC

    更新日期：2010-07-30 00:00:00

  • A branched pathway governing the activation of a developmental transcription factor by regulated intramembrane proteolysis.

    abstract：:The proteolytic activation of the membrane-associated transcription factor pro-sigma(K) is controlled by a signal transduction pathway during sporulation in the bacterium Bacillus subtilis. The pro-sigma(K) processing enzyme SpoIVFB, a membrane-embedded metalloprotease, is held inactive by two other integral-membrane ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2006.05.019

    authors： Campo N,Rudner DZ

    更新日期：2006-07-07 00:00:00

  • The mechanism of a neurotransmitter:sodium symporter--inward release of Na+ and substrate is triggered by substrate in a second binding site.

    abstract：:Eukaryotic neurotransmitter:sodium symporters (NSSs), targets for antidepressants and psychostimulants, terminate neurotransmission by sodium-driven reuptake. The crystal structure of LeuT(Aa), a prokaryotic NSS homolog, revealed an occluded state in which one leucine and two Na(+) ions are bound, but provided limited...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2008.05.008

    authors： Shi L,Quick M,Zhao Y,Weinstein H,Javitch JA

    更新日期：2008-06-20 00:00:00

  • DNA Breaks and End Resection Measured Genome-wide by End Sequencing.

    abstract：:DNA double-strand breaks (DSBs) arise during physiological transcription, DNA replication, and antigen receptor diversification. Mistargeting or misprocessing of DSBs can result in pathological structural variation and mutation. Here we describe a sensitive method (END-seq) to monitor DNA end resection and DSBs genome...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2016.06.034

    authors： Canela A,Sridharan S,Sciascia N,Tubbs A,Meltzer P,Sleckman BP,Nussenzweig A

    更新日期：2016-09-01 00:00:00

  • Properties of Stress Granule and P-Body Proteomes.

    abstract：:Stress granules and P-bodies are cytosolic biomolecular condensates that dynamically form by the phase separation of RNAs and proteins. They participate in translational control and buffer the proteome. Upon stress, global translation halts and mRNAs bound to the translational machinery and other proteins coalesce to ...

    journal_title：Molecular cell

    pub_type： 杂志文章,评审

    doi：10.1016/j.molcel.2019.09.014

    authors： Youn JY,Dyakov BJA,Zhang J,Knight JDR,Vernon RM,Forman-Kay JD,Gingras AC

    更新日期：2019-10-17 00:00:00

  • Essential role of ERK dimers in the activation of cytoplasmic but not nuclear substrates by ERK-scaffold complexes.

    abstract：:Signals transmitted by ERK MAP kinases regulate the functions of multiple substrates present in the nucleus and in the cytoplasm. ERK signals are optimized by scaffold proteins that modulate their intensity and spatial fidelity. Once phosphorylated, ERKs dimerize, but how dimerization impacts on the activation of the ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2008.07.024

    authors： Casar B,Pinto A,Crespo P

    更新日期：2008-09-05 00:00:00

  • An argonaute protein directs nuclear Xrn2 function.

    abstract：:Argonaute proteins are the mediators of small RNA-guided gene silencing pathways. In this issue, Couvillion and coworkers (Couvillion et al., 2012) found an unexpected function for a Tetrahymena Argonaute protein: It forms a complex with tRNA fragments and is required for nuclear Xrn2 localization and function. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2012.11.015

    authors： Hasler D,Meister G

    更新日期：2012-11-30 00:00:00

  • Nucleosomes are context-specific, H2A.Z-modulated barriers to RNA polymerase.

    abstract：:Nucleosomes are barriers to transcription in vitro; however, their effects on RNA polymerase in vivo are unknown. Here we describe a simple and general strategy to comprehensively map the positions of elongating and arrested RNA polymerase II (RNAPII) at nucleotide resolution. We find that the entry site of the first ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2014.02.014

    authors： Weber CM,Ramachandran S,Henikoff S

    更新日期：2014-03-06 00:00:00

  • Transient DNA Occupancy of the SMC Interarm Space in Prokaryotic Condensin.

    abstract：:Multi-subunit SMC ATPases control chromosome superstructure and DNA topology, presumably by DNA translocation and loop extrusion. Chromosomal DNA is entrapped within the tripartite SMCkleisin ring. Juxtaposed SMC heads ("J heads") or engaged SMC heads ("E heads") split the SMCkleisin ring into "S" and "K" sub-compartm...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2019.05.001

    authors： Vazquez Nunez R,Ruiz Avila LB,Gruber S

    更新日期：2019-07-25 00:00:00

  • Suv39h-dependent H3K9me3 marks intact retrotransposons and silences LINE elements in mouse embryonic stem cells.

    abstract：:Heterochromatin is required to restrict aberrant expression of retrotransposons, but it remains poorly defined due to the underlying repeat-rich sequences. We dissected Suv39h-dependent histone H3 lysine 9 trimethylation (H3K9me3) by genome-wide ChIP sequencing in mouse embryonic stem cells (ESCs). Refined bioinformat...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2014.05.029

    authors： Bulut-Karslioglu A,De La Rosa-Velázquez IA,Ramirez F,Barenboim M,Onishi-Seebacher M,Arand J,Galán C,Winter GE,Engist B,Gerle B,O'Sullivan RJ,Martens JH,Walter J,Manke T,Lachner M,Jenuwein T

    更新日期：2014-07-17 00:00:00

  • Lysyl Oxidase 3 Is a Dual-Specificity Enzyme Involved in STAT3 Deacetylation and Deacetylimination Modulation.

    abstract：:In mammalian cells, histone deacetylase (HDAC) and Sirtuin (SIRT) are two families responsible for removing acetyl groups from acetylated proteins. Here, we describe protein deacetylation coupled with deacetylimination as a function of lysyl oxidase (LOX) family members. LOX-like 3 (Loxl3) associates with Stat3 in the...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2016.12.002

    authors： Ma L,Huang C,Wang XJ,Xin DE,Wang LS,Zou QC,Zhang YS,Tan MD,Wang YM,Zhao TC,Chatterjee D,Altura RA,Wang C,Xu YS,Yang JH,Fan YS,Han BH,Si J,Zhang X,Cheng J,Chang Z,Chin YE

    更新日期：2017-01-19 00:00:00

  • p58(IPK)-mediated attenuation of the proapoptotic PERK-CHOP pathway allows malignant progression upon low glucose.

    abstract：:As solid tumors expand, oxygen and nutrients become limiting owing to inadequate vascularization and diffusion. How malignant cells cope with this potentially lethal metabolic stress remains poorly understood. We found that glucose shortage associated with malignant progression triggers apoptosis through the endoplasm...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2013.01.009

    authors： Huber AL,Lebeau J,Guillaumot P,Pétrilli V,Malek M,Chilloux J,Fauvet F,Payen L,Kfoury A,Renno T,Chevet E,Manié SN

    更新日期：2013-03-28 00:00:00

  • The response regulator BvgA and RNA polymerase alpha subunit C-terminal domain bind simultaneously to different faces of the same segment of promoter DNA.

    abstract：:Examination of the binding of FeBABE-conjugated BvgA to the fha promoter of Bordetella pertussis has revealed that three dimers, formed by head-to-head association of monomers, bind one face of the DNA helix from the inverted-heptad primary binding site to the -35 region. The orientation of BvgA monomers within the di...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(03)00007-8

    authors： Boucher PE,Maris AE,Yang MS,Stibitz S

    更新日期：2003-01-01 00:00:00

  • Differential SUMOylation of LXRalpha and LXRbeta mediates transrepression of STAT1 inflammatory signaling in IFN-gamma-stimulated brain astrocytes.

    abstract：:To unravel the roles of LXRs in inflammation and immunity, we examined the function of LXRs in development of IFN-gamma-mediated inflammation using cultured rat brain astrocytes. LXR ligands inhibit neither STAT1 phosphorylation nor STAT1 translocation to the nucleus but, rather, inhibit STAT1 binding to promoters and...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2009.07.021

    authors： Lee JH,Park SM,Kim OS,Lee CS,Woo JH,Park SJ,Joe EH,Jou I

    更新日期：2009-09-24 00:00:00

  • A core complex of CPSF73, CPSF100, and Symplekin may form two different cleavage factors for processing of poly(A) and histone mRNAs.

    abstract：:Metazoan histone mRNAs are unique: their pre-mRNAs contain no introns, and the mRNAs are not polyadenylated, ending instead in a conserved stem-loop structure. In Drosophila, canonical poly(A) signals are located downstream of the normal cleavage site of each histone gene and are utilized when histone 3' end formation...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2009.04.024

    authors： Sullivan KD,Steiniger M,Marzluff WF

    更新日期：2009-05-15 00:00:00

  • Principles and properties of eukaryotic mRNPs.

    abstract：:The proper processing, export, localization, translation, and degradation of mRNAs are necessary for regulation of gene expression. These processes are controlled by mRNA-specific regulatory proteins, noncoding RNAs, and core machineries common to most mRNAs. These factors bind the mRNA in large complexes known as mes...

    journal_title：Molecular cell

    pub_type： 杂志文章,评审

    doi：10.1016/j.molcel.2014.04.033

    authors： Mitchell SF,Parker R

    更新日期：2014-05-22 00:00:00

  • UneCLIPsing HuR nuclear function.

    abstract：:The RNA-binding protein HuR, while known to stabilize cytoplasmic mRNAs, is largely nuclear. In this issue of Molecular Cell, Mukherjee et al. (2011) and Lebedeva et al. (2011) identify transcriptome-wide HuR-RNA interactions using PAR-CLIP, unveiling HuR's nuclear role in pre-mRNA processing. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2011.07.016

    authors： Srikantan S,Gorospe M

    更新日期：2011-08-05 00:00:00

  • MCM8- and MCM9-deficient mice reveal gametogenesis defects and genome instability due to impaired homologous recombination.

    abstract：:We generated knockout mice for MCM8 and MCM9 and show that deficiency for these genes impairs homologous recombination (HR)-mediated DNA repair during gametogenesis and somatic cells cycles. MCM8(-/-) mice are sterile because spermatocytes are blocked in meiotic prophase I, and females have only arrested primary folli...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2012.05.048

    authors： Lutzmann M,Grey C,Traver S,Ganier O,Maya-Mendoza A,Ranisavljevic N,Bernex F,Nishiyama A,Montel N,Gavois E,Forichon L,de Massy B,Méchali M

    更新日期：2012-08-24 00:00:00

  • RNase H and multiple RNA biogenesis factors cooperate to prevent RNA:DNA hybrids from generating genome instability.

    abstract：:Genome instability, a hallmark of cancer progression, is thought to arise through DNA double strand breaks (DSBs). Studies in yeast and mammalian cells have shown that DSBs and instability can occur through RNA:DNA hybrids generated by defects in RNA elongation and splicing. We report that in yeast hybrids naturally f...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2011.10.017

    authors： Wahba L,Amon JD,Koshland D,Vuica-Ross M

    更新日期：2011-12-23 00:00:00

  • Histone Acetyltransferase 1 Links Metabolism and Transcription to Cell-Cycle Progression.

    abstract：:HAT1 is an essential gene in mammals that acetylates newly synthesized histone H4 and is involved in chromatin assembly and genome maintenance. In this issue of Molecular Cell, Gruber et al. (2019) show that HAT1 binds to and stimulates H4 gene expression in a growth-factor- and glucose-dependent manner. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2019.08.004

    authors： Denu JM

    更新日期：2019-08-22 00:00:00

  • Smad proteins bind a conserved RNA sequence to promote microRNA maturation by Drosha.

    abstract：:The signal transducers of the transforming growth factor beta (TGFbeta)/bone morphogenetic protein (BMP), the Smads, promote the expression of a subset of miRNAs by facilitating the cleavage reaction by Drosha. The mechanism that limits Smad-mediated processing to a selective group of miRNAs remained hitherto unexplor...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2010.07.011

    authors： Davis BN,Hilyard AC,Nguyen PH,Lagna G,Hata A

    更新日期：2010-08-13 00:00:00

  • A Dicer-Independent Route for Biogenesis of siRNAs that Direct DNA Methylation in Arabidopsis.

    abstract：:DNA methylation directed by 24-nucleotide (nt) small interfering RNAs (siRNAs) plays critical roles in gene regulation and transposon silencing in Arabidopsis. 24-nt siRNAs are known to be processed from double-stranded RNAs by Dicer-like 3 (DCL3) and loaded into the effector Argonaute 4 (AGO4). Here we report a disti...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2015.11.015

    authors： Ye R,Chen Z,Lian B,Rowley MJ,Xia N,Chai J,Li Y,He XJ,Wierzbicki AT,Qi Y

    更新日期：2016-01-21 00:00:00

  • Activation of RNA polymerase I transcription by cockayne syndrome group B protein and histone methyltransferase G9a.

    abstract：:Cockayne syndrome group B (CSB) protein plays a role in both transcription-coupled DNA repair and transcriptional regulation of all three classes of nuclear RNA polymerases. Here we show that a complex consisting of CSB, RNA polymerase I (Pol I), and histone methyltransferase G9a is present at active rRNA genes. G9a m...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2007.06.021

    authors： Yuan X,Feng W,Imhof A,Grummt I,Zhou Y

    更新日期：2007-08-17 00:00:00

  • Fluorescence Amplification Method for Forward Genetic Discovery of Factors in Human mRNA Degradation.

    abstract：:Nonsense-mediated decay (NMD) degrades mRNAs containing a premature termination codon (PTC). PTCs are a frequent cause of human genetic diseases, and the NMD pathway is known to modulate disease severity. Since partial NMD attenuation can potentially enhance nonsense suppression therapies, better definition of human-s...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2016.11.032

    authors： Alexandrov A,Shu MD,Steitz JA

    更新日期：2017-01-05 00:00:00

  • HDAC6 modulates cell motility by altering the acetylation level of cortactin.

    abstract：:Histone deacetylase 6 (HDAC6) is a tubulin-specific deacetylase that regulates microtubule-dependent cell movement. In this study, we identify the F-actin-binding protein cortactin as a HDAC6 substrate. We demonstrate that HDAC6 binds cortactin and that overexpression of HDAC6 leads to hypoacetylation of cortactin, wh...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2007.05.033

    authors： Zhang X,Yuan Z,Zhang Y,Yong S,Salas-Burgos A,Koomen J,Olashaw N,Parsons JT,Yang XJ,Dent SR,Yao TP,Lane WS,Seto E

    更新日期：2007-07-20 00:00:00

  • A transient heterochromatic state in Xist preempts X inactivation choice without RNA stabilization.

    abstract：:X chromosome inactivation (XCI) depends on a noncoding sense-antisense transcript pair, Xist and Tsix. At the onset of XCI, Xist RNA accumulates on one of two Xs, coating and silencing the chromosome in cis. The molecular basis for monoallelic Xist upregulation is not known, though evidence predominantly supports a po...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2006.01.028

    authors： Sun BK,Deaton AM,Lee JT

    更新日期：2006-03-03 00:00:00

  • Arginine methylation of FOXO transcription factors inhibits their phosphorylation by Akt.

    abstract：:Forkhead box O (FOXO) transcription factors, the key regulators of cell survival, are negatively controlled through the PI3K-Akt signaling pathway. Phosphorylation of FOXO by Akt leads to cytoplasmic localization and subsequent degradation via the ubiquitin-proteasome system. Here we show a paradigm of FOXO1 regulatio...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2008.09.013

    authors： Yamagata K,Daitoku H,Takahashi Y,Namiki K,Hisatake K,Kako K,Mukai H,Kasuya Y,Fukamizu A

    更新日期：2008-10-24 00:00:00

  • Actin homolog MreB affects chromosome segregation by regulating topoisomerase IV in Escherichia coli.

    abstract：:In Escherichia coli, topoisomerase IV, a type II topoisomerase, mediates the resolution of topological linkages between replicated daughter chromosomes and is essential for chromosome segregation. Topo IV activity is restricted to only a short interval late in the cell cycle. However, the mechanism that confers this t...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2009.01.001

    authors： Madabhushi R,Marians KJ

    更新日期：2009-01-30 00:00:00

  • Structural and functional versatility of the FHA domain in DNA-damage signaling by the tumor suppressor kinase Chk2.

    abstract：:The Chk2 Ser/Thr kinase plays crucial, evolutionarily conserved roles in cellular responses to DNA damage. Identification of two pro-oncogenic mutations within the Chk2 FHA domain has highlighted its importance for Chk2 function in checkpoint activation. The X-ray structure of the Chk2 FHA domain in complex with an in...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(02)00527-0

    authors： Li J,Williams BL,Haire LF,Goldberg M,Wilker E,Durocher D,Yaffe MB,Jackson SP,Smerdon SJ

    更新日期：2002-05-01 00:00:00

  • A dynamic role of HAUSP in the p53-Mdm2 pathway.

    abstract：:Our previous study showed that ubiquitination of p53 is reversible and that the ubiquitin hydrolase HAUSP can stabilize p53 by deubiquitination. Here, we found that partial reduction of endogenous HAUSP levels by RNAi indeed destabilizes endogenous p53; surprisingly, however, nearly complete ablation of HAUSP stabiliz...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(04)00157-1

    authors： Li M,Brooks CL,Kon N,Gu W

    更新日期：2004-03-26 00:00:00