Abstract：
:Bursts of nascent mRNA have been shown to lead to substantial cell-cell variation in unicellular organisms, facilitating diverse responses to environmental challenges. It is unknown whether similar bursts and gene-expression noise occur in mammalian tissues. To address this, we combine single molecule transcript counting with dual-color labeling and quantification of nascent mRNA to characterize promoter states, transcription rates, and transcript lifetimes in the intact mouse liver. We find that liver gene expression is highly bursty, with promoters stochastically switching between transcriptionally active and inactive states. Promoters of genes with short mRNA lifetimes are active longer, facilitating rapid response while reducing burst-associated noise. Moreover, polyploid hepatocytes exhibit less noise than diploid hepatocytes, suggesting a possible benefit to liver polyploidy. Thus, temporal averaging and liver polyploidy dampen the intrinsic variability associated with transcriptional bursts. Our approach can be used to study transcriptional bursting in diverse mammalian tissues.
journal_name
Mol Celljournal_title
Molecular cellauthors
Bahar Halpern K,Tanami S,Landen S,Chapal M,Szlak L,Hutzler A,Nizhberg A,Itzkovitz Sdoi
10.1016/j.molcel.2015.01.027subject
Has Abstractpub_date
2015-04-02 00:00:00pages
147-56issue
1eissn
1097-2765issn
1097-4164pii
S1097-2765(15)00050-7journal_volume
58pub_type
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