Abstract：
:In eukaryotes, tRNAs are synthesized in the nucleus and after several maturation steps exported to the cytoplasm. Here, we identify exportin-t as a specific mediator of tRNA export. It is a RanGTP-binding, importin beta-related factor with predominantly nuclear localization. It shuttles rapidly between nucleus and cytoplasm and interacts with nuclear pore complexes. Exportin-t binds tRNA directly and with high affinity. Its cellular concentration in Xenopus oocytes was found to be rate-limiting for export of all tRNAs tested, as judged by microinjection experiments. RanGTP regulates the substrate-exportin-t interaction such that tRNA can be preferentially bound in the nucleus and released in the cytoplasm.
journal_name
Mol Celljournal_title
Molecular cellauthors
Kutay U,Lipowsky G,Izaurralde E,Bischoff FR,Schwarzmaier P,Hartmann E,Görlich Ddoi
10.1016/s1097-2765(00)80036-2subject
Has Abstractpub_date
1998-02-01 00:00:00pages
359-69issue
3eissn
1097-2765issn
1097-4164pii
S1097-2765(00)80036-2journal_volume
1pub_type
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