Yeast unravels epigenetic apoptosis control: deadly chat within a histone tail.

abstract：:Metazoan histone mRNAs end in a highly conserved stem-loop structure followed by ACCCA. Previous studies have suggested that the stem-loop binding protein (SLBP) is the only protein binding this region. Using RNA affinity purification, we identified a second protein, designated 3'hExo, that contains a SAP and a 3' exo...

journal_title：Molecular cell

authors： Dominski Z,Yang XC,Kaygun H,Dadlez M,Marzluff WF

更新日期：2003-08-01 00:00:00

abstract：:Triplex structure-forming GAA/TTC repeats pose a dual threat to the eukaryotic genome integrity. Their potential to expand can lead to gene inactivation, the cause of Friedreich's ataxia disease in humans. In model systems, long GAA/TTC tracts also act as chromosomal fragile sites that can trigger gross chromosomal re...

journal_title：Molecular cell

authors： Zhang Y,Shishkin AA,Nishida Y,Marcinkowski-Desmond D,Saini N,Volkov KV,Mirkin SM,Lobachev KS

更新日期：2012-10-26 00:00:00

abstract：:Protein synthesis is a major target within the bacterial cell for antibiotics. Investigations into ribosome-targeting antibiotics have provided much needed functional and structural insight into their mechanism of action. However, the increasing prevalence of multi-drug-resistant bacteria has limited the utility of ou...

journal_title：Molecular cell

authors： Arenz S,Wilson DN

更新日期：2016-01-07 00:00:00

abstract：:In this issue of Molecular Cell, Zhang et al. and Møller et al. independently report studies of the E. coli Hfq protein, revealing significant sequence similarities with human Sm proteins. Their findings suggest that Hfq, and the Sm and Sm-like (Lsm) proteins, may function in stabilizing interactions between RNA molec...

journal_title：Molecular cell

authors： Donahue WF,Jarrell KA

更新日期：2002-01-01 00:00:00

abstract：:It has been suspected that cell-cycle progression might be functionally coupled with RNA processing. However, little is known about the role of the precise splicing control in cell-cycle progression. Here, we report that SON, a large Ser/Arg (SR)-related protein, is a splicing cofactor contributing to efficient splici...

journal_title：Molecular cell

authors： Ahn EY,DeKelver RC,Lo MC,Nguyen TA,Matsuura S,Boyapati A,Pandit S,Fu XD,Zhang DE

更新日期：2011-04-22 00:00:00

abstract：:Linkage-specific polyubiquitin recognition is thought to make possible the diverse set of functional outcomes associated with ubiquitination. Thus far, mechanistic insight into this selectivity has been largely limited to single domains that preferentially bind to lysine 48-linked polyubiquitin (K48-polyUb) in isolati...

journal_title：Molecular cell

authors： Sims JJ,Cohen RE

更新日期：2009-03-27 00:00:00

abstract：:Perfringolysin O, a bacterial cytolytic toxin, forms unusually large pores in cholesterol-containing membranes by the spontaneous insertion of two of its four domains into the bilayer. By monitoring the kinetics of domain-specific conformational changes and pore formation using fluorescence spectroscopy, the temporal ...

journal_title：Molecular cell

authors： Heuck AP,Hotze EM,Tweten RK,Johnson AE

更新日期：2000-11-01 00:00:00

abstract：:Pre-mRNA 3' end formation is an essential step in eukaryotic gene expression. Over half of human genes produce alternatively polyadenylated mRNAs, suggesting that regulated polyadenylation is an important mechanism for posttranscriptional gene control. Although a number of mammalian mRNA 3' processing factors have bee...

journal_title：Molecular cell

authors： Shi Y,Di Giammartino DC,Taylor D,Sarkeshik A,Rice WJ,Yates JR 3rd,Frank J,Manley JL

更新日期：2009-02-13 00:00:00

abstract：:DNA methylation directed by 24-nucleotide (nt) small interfering RNAs (siRNAs) plays critical roles in gene regulation and transposon silencing in Arabidopsis. 24-nt siRNAs are known to be processed from double-stranded RNAs by Dicer-like 3 (DCL3) and loaded into the effector Argonaute 4 (AGO4). Here we report a disti...

journal_title：Molecular cell

authors： Ye R,Chen Z,Lian B,Rowley MJ,Xia N,Chai J,Li Y,He XJ,Wierzbicki AT,Qi Y

更新日期：2016-01-21 00:00:00

abstract：:Genome-wide profiling of histone modifications can provide systematic insight into the regulatory elements and programs engaged in a given cell type. However, conventional chromatin immunoprecipitation and sequencing (ChIP-seq) does not capture quantitative information on histone modification levels, requires large am...

journal_title：Molecular cell

authors： van Galen P,Viny AD,Ram O,Ryan RJ,Cotton MJ,Donohue L,Sievers C,Drier Y,Liau BB,Gillespie SM,Carroll KM,Cross MB,Levine RL,Bernstein BE

更新日期：2016-01-07 00:00:00

abstract：:The dynamic protein interactions required for transcription are functionally important yet poorly understood; in this issue of Molecular Cell, Zobeck et al. (2010) resolve the sequential recruitment and selective recycling of transcription factors at an actively transcribing locus in Drosophila. ...

journal_title：Molecular cell

authors： Motta-Mena LB,Partch CL,Gardner KH

更新日期：2010-12-22 00:00:00

abstract：:We have developed a highly parallel strategy, systematic gene-to-phenotype arrays (SGPAs), to comprehensively map the genetic landscape driving molecular phenotypes of interest. By this approach, a complete yeast genetic mutant array is crossed with fluorescent reporters and imaged on membranes at high density and con...

journal_title：Molecular cell

authors： Jaeger PA,Ornelas L,McElfresh C,Wong LR,Hampton RY,Ideker T

更新日期：2018-01-18 00:00:00

abstract：:In Xer site-specific recombination, sequential DNA strand exchange reactions are catalyzed by a heterotetrameric complex composed of two recombinases, XerC and XerD. It is demonstrated that XerC and XerD catalytic activity is controlled by an interaction involving the C-terminal end of each protein (the donor region) ...

journal_title：Molecular cell

authors： Hallet B,Arciszewska LK,Sherratt DJ

更新日期：1999-12-01 00:00:00

abstract：:Nine human neurodegenerative diseases are due to expansion of a CAG repeat- encoding glutamine within the open reading frame of the respective genes. Polyglutamine (polyQ) expansion confers dominant toxicity, resulting in neuronal degeneration. MicroRNAs (miRNAs) have been shown to modulate programmed cell death durin...

journal_title：Molecular cell

authors： Bilen J,Liu N,Burnett BG,Pittman RN,Bonini NM

更新日期：2006-10-06 00:00:00

abstract：:The CCCH finger protein PIE-1 is a regulator of germ cell fate that segregates with the germ lineage in early embryos. At each asymmetric division, PIE-1 is inherited preferentially by the germline daughter and is excluded from the somatic daughter. We show that this asymmetry is regulated at the protein level by two ...

journal_title：Molecular cell

authors： Reese KJ,Dunn MA,Waddle JA,Seydoux G

更新日期：2000-08-01 00:00:00

abstract：:In Escherichia coli, topoisomerase IV, a type II topoisomerase, mediates the resolution of topological linkages between replicated daughter chromosomes and is essential for chromosome segregation. Topo IV activity is restricted to only a short interval late in the cell cycle. However, the mechanism that confers this t...

journal_title：Molecular cell

authors： Madabhushi R,Marians KJ

更新日期：2009-01-30 00:00:00

abstract：:The separation of sister chromatids in anaphase depends on the dissociation of cohesin from chromosomes. In vertebrates, some cohesin is removed from chromosomes at the onset of anaphase by proteolytic cleavage. In contrast, the bulk of cohesin is removed from chromosomes already in prophase and prometaphase by an unk...

journal_title：Molecular cell

authors： Sumara I,Vorlaufer E,Stukenberg PT,Kelm O,Redemann N,Nigg EA,Peters JM

更新日期：2002-03-01 00:00:00

abstract：:Poly(ADP-ribose) polymerase-1 (PARP-1) creates the posttranslational modification PAR from substrate NAD(+) to regulate multiple cellular processes. DNA breaks sharply elevate PARP-1 catalytic activity to mount a cell survival repair response, whereas persistent PARP-1 hyperactivation during severe genotoxic stress is...

journal_title：Molecular cell

authors： Dawicki-McKenna JM,Langelier MF,DeNizio JE,Riccio AA,Cao CD,Karch KR,McCauley M,Steffen JD,Black BE,Pascal JM

更新日期：2015-12-03 00:00:00

abstract：:Autophagy is required for benign hepatic tumors to progress into malignant hepatocellular carcinoma. However, the mechanism is unclear. Here, we report that mitophagy, the selective removal of mitochondria by autophagy, positively regulates hepatic cancer stem cells (CSCs) by suppressing the tumor suppressor p53. When...

journal_title：Molecular cell

authors： Liu K,Lee J,Kim JY,Wang L,Tian Y,Chan ST,Cho C,Machida K,Chen D,Ou JJ

更新日期：2017-10-19 00:00:00

abstract：:The WTX gene is frequently lost or mutated in Wilms tumor. In this issue of Molecular Cell, Kim et al. (2012) identify WTX modulation of p53 tumor-suppressor activity through regulation of p53 acetylation. Therefore, WTX differentially regulates the oncogenic β-catenin pathway and the tumor-suppressing p53 pathway. ...

journal_title：Molecular cell

authors： Dai C,Gu W

更新日期：2012-03-09 00:00:00

abstract：:In this issue of Molecular Cell, Roberts-Galbraith and colleagues report that a key cytokinetic regulator in fission yeast, Cdc15, is phosphorylated on numerous sites that collectively, but not individually, control its oligomerization state and its associations with the plasma membrane and interacting proteins. ...

journal_title：Molecular cell

authors： Glotzer M

更新日期：2010-07-09 00:00:00

abstract：:Mammalian target of rapamycin (mTOR) activity is regulated by assembly of two functionally distinct complexes, mTORC1 and mTORC2. In syndecan-4 (S4) null endothelial cells, mTORC2 activity is reduced, resulting in decreased Akt activation, while mTORC1 activity is increased. Levels of rictor, mLST8, and mSin-1 are unc...

journal_title：Molecular cell

authors： Partovian C,Ju R,Zhuang ZW,Martin KA,Simons M

更新日期：2008-10-10 00:00:00

abstract：:A recent paper by Gallagher et al. (2020) demonstrates that c-di-GMP controls spore formation in Streptomyces venezuelae through sequestering the sporulation sigma factor σWhiG and presents the crystal structure of a ternary complex between c-di-GMP, σWhiG, and its anti-sigma factor, RsiG. ...

journal_title：Molecular cell

authors： Chou SH,Galperin MY

更新日期：2020-02-06 00:00:00

abstract：:The nuclear hormone receptor PPAR gamma promotes adipogenesis and macrophage differentiation and is a primary pharmacological target in the treatment of type II diabetes. Here, we show that PPAR gamma gene knockout results in two independent lethal phases. Initially, PPAR gamma deficiency interferes with terminal diff...

journal_title：Molecular cell

authors： Barak Y,Nelson MC,Ong ES,Jones YZ,Ruiz-Lozano P,Chien KR,Koder A,Evans RM

更新日期：1999-10-01 00:00:00

abstract：:Faithful copying of the genome is essential for life. In eukaryotes, a single archaeo-eukaryotic primase (AEP), DNA primase, is required for the initiation and progression of DNA replication. Here we have identified additional eukaryotic AEP-like proteins with DNA-dependent primase and/or polymerase activity. Uniquely...

journal_title：Molecular cell

authors： Rudd SG,Glover L,Jozwiakowski SK,Horn D,Doherty AJ

更新日期：2013-11-21 00:00:00

abstract：:Stress granules (SGs) are condensates of mRNPs that form in response to stress. SGs arise by multivalent protein-protein, protein-RNA, and RNA-RNA interactions. However, the role of RNA-RNA interactions in SG assembly remains understudied. Here, we describe a yeast SG reconstitution system that faithfully recapitulate...

journal_title：Molecular cell

authors： Begovich K,Wilhelm JE

更新日期：2020-09-17 00:00:00

abstract：:The guanidyl moiety is a component of fundamental metabolites, including the amino acid arginine, the energy carrier creatine, and the nucleobase guanine. Curiously, reports regarding the importance of free guanidine in biology are sparse, and no biological receptors that specifically recognize this compound have been...

journal_title：Molecular cell

authors： Nelson JW,Atilho RM,Sherlock ME,Stockbridge RB,Breaker RR

更新日期：2017-01-19 00:00:00

abstract：:The identification of metazoan origins of DNA replication has so far been hampered by the lack of a suitable genetic screening and by the cumbersomeness of the currently available mapping procedures. Here we describe the construction of a library of nascent DNA, representative of all cellular origin sequences, and its...

journal_title：Molecular cell

authors： Todorovic V,Giadrossi S,Pelizon C,Mendoza-Maldonado R,Masai H,Giacca M

更新日期：2005-08-19 00:00:00

abstract：:In this issue of Molecular Cell, Chen et al. (2014) provide evidence that FMRP represses translation by binding the ribosome, suggesting a novel form of translational control. ...

journal_title：Molecular cell

authors： Harigaya Y,Parker R

更新日期：2014-05-08 00:00:00

abstract：:In this issue of Molecular Cell, detail molecular events that trigger apoptosis following growth factor withdrawal, linking irreversible mitochondrial permeabilization to activation of GSK-3 and subsequent phosphorylation, ubiquitinylation, and degradation of the antiapoptotic BCL-2 family member MCL-1. ...

journal_title：Molecular cell

authors： Letai A

更新日期：2006-03-17 00:00:00