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Adaptation in CRISPR-Cas Systems.

Abstract：

:Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins constitute an adaptive immune system in prokaryotes. The system preserves memories of prior infections by integrating short segments of foreign DNA, termed spacers, into the CRISPR array in a process termed adaptation. During the past 3 years, significant progress has been made on the genetic requirements and molecular mechanisms of adaptation. Here we review these recent advances, with a focus on the experimental approaches that have been developed, the insights they generated, and a proposed mechanism for self- versus non-self-discrimination during the process of spacer selection. We further describe the regulation of adaptation and the protein players involved in this fascinating process that allows bacteria and archaea to harbor adaptive immunity.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Sternberg SH,Richter H,Charpentier E,Qimron U

doi

10.1016/j.molcel.2016.01.030

subject

Has Abstract

pub_date

2016-03-17 00:00:00

pages

797-808

issue

6

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(16)00083-6

journal_volume

61

pub_type

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