Abstract：
:The Salmonellae PhoQ sensor kinase senses the mammalian phagosome environment to activate a transcriptional program essential for virulence. The PhoQ periplasmic domain binds divalent cations, forming bridges with inner membrane phospholipids to maintain PhoQ repression. PhoQ also binds and is activated by cationic antimicrobial peptides. In this work, PhoQ is directly activated by exposure of the sensor domain to pH 5.5. NMR spectroscopy indicates that at acidic pH, the PhoQ periplasmic domain adopts a conformation different from that in the presence of divalent cations or antimicrobial peptides. The conformation is partially simulated by mutation of histidine 157, which is part of an interaction network that distinguishes the repressed conformation. The effects of antimicrobial peptides and pH on PhoQ activity are additive. We propose a model of activation by antimicrobial peptides via disruption of the cation bridges and/or by acidification of the periplasm through destabilization of the interaction network.
journal_name
Mol Celljournal_title
Molecular cellauthors
Prost LR,Daley ME,Le Sage V,Bader MW,Le Moual H,Klevit RE,Miller SIdoi
10.1016/j.molcel.2007.03.008subject
Has Abstractpub_date
2007-04-27 00:00:00pages
165-74issue
2eissn
1097-2765issn
1097-4164pii
S1097-2765(07)00153-0journal_volume
26pub_type
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