当前位置： SCI文献检索 > MOLECULAR CELL期刊下所有文献 > Residue-by-Residue View of In Vitro FUS Granules that Bind the C-Terminal Domain of RNA Polymerase II.

Residue-by-Residue View of In Vitro FUS Granules that Bind the C-Terminal Domain of RNA Polymerase II.

Abstract：

:Phase-separated states of proteins underlie ribonucleoprotein (RNP) granules and nuclear RNA-binding protein assemblies that may nucleate protein inclusions associated with neurodegenerative diseases. We report that the N-terminal low-complexity domain of the RNA-binding protein Fused in Sarcoma (FUS LC) is structurally disordered and forms a liquid-like phase-separated state resembling RNP granules. This state directly binds the C-terminal domain of RNA polymerase II. Phase-separated FUS lacks static structures as probed by fluorescence microscopy, indicating they are distinct from both protein inclusions and hydrogels. We use solution nuclear magnetic resonance spectroscopy to directly probe the dynamic architecture within FUS liquid phase-separated assemblies. Importantly, we find that FUS LC retains disordered secondary structure even in the liquid phase-separated state. Therefore, we propose that disordered protein granules, even those made of aggregation-prone prion-like domains, are dynamic and disordered molecular assemblies with transiently formed protein-protein contacts.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Burke KA,Janke AM,Rhine CL,Fawzi NL

doi

10.1016/j.molcel.2015.09.006

subject

Has Abstract

pub_date

2015-10-15 00:00:00

pages

231-41

issue

2

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(15)00702-9

journal_volume

60

pub_type

杂志文章

相关文献

MOLECULAR CELL文献大全
  • Crystal structure and deletion analysis show that the accessory subunit of mammalian DNA polymerase gamma, Pol gamma B, functions as a homodimer.

    abstract：:Polymerase gamma, which replicates and repairs mitochondrial DNA, requires the Pol gamma B subunit for processivity. We determined the crystal structure of mouse Pol gamma B, a core component of the mitochondrial replication machinery. Pol gamma B shows high similarity to glycyl-tRNA synthetase and dimerizes through a...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(01)00153-8

    authors： Carrodeguas JA,Theis K,Bogenhagen DF,Kisker C

    更新日期：2001-01-01 00:00:00

  • Ribosomal Proteins Regulate MHC Class I Peptide Generation for Immunosurveillance.

    abstract：:The MHC class I antigen presentation system enables T cell immunosurveillance of cancers and viruses. A substantial fraction of the immunopeptidome derives from rapidly degraded nascent polypeptides (DRiPs). By knocking down each of the 80 ribosomal proteins, we identified proteins that modulate peptide generation wit...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2018.12.020

    authors： Wei J,Kishton RJ,Angel M,Conn CS,Dalla-Venezia N,Marcel V,Vincent A,Catez F,Ferré S,Ayadi L,Marchand V,Dersh D,Gibbs JS,Ivanov IP,Fridlyand N,Couté Y,Diaz JJ,Qian SB,Staudt LM,Restifo NP,Yewdell JW

    更新日期：2019-03-21 00:00:00

  • Proteasome active sites allosterically regulate each other, suggesting a cyclical bite-chew mechanism for protein breakdown.

    abstract：:In eukaryotes, the 20S proteasome contains two chymotrypsin-like, two trypsin-like, and two active sites shown here to have caspase-like specificity. We report that certain sites allosterically regulate each other's activities. Substrates of a chymotrypsin-like site stimulate dramatically the caspase-like activity and...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(00)80341-x

    authors： Kisselev AF,Akopian TN,Castillo V,Goldberg AL

    更新日期：1999-09-01 00:00:00

  • Inhibition of both the extrinsic and intrinsic death pathways through nonhomotypic death-fold interactions.

    abstract：:Death-fold domains constitute an evolutionarily conserved superfamily that mediates apoptotic signaling. These motifs, including CARD (caspase recruitment domain), DD (death domain), and DED (death effector domain), are believed to exert their effects solely through homotypic interactions. Herein we demonstrate that t...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2004.08.020

    authors： Nam YJ,Mani K,Ashton AW,Peng CF,Krishnamurthy B,Hayakawa Y,Lee P,Korsmeyer SJ,Kitsis RN

    更新日期：2004-09-24 00:00:00

  • SMADs and YAP compete to control elongation of β-catenin:LEF-1-recruited RNAPII during hESC differentiation.

    abstract：:The Wnt3a/β-catenin and Activin/SMAD2,3 signaling pathways synergize to induce endodermal differentiation of human embryonic stem cells; however, the underlying mechanism is not well understood. Using ChIP-seq and GRO-seq analyses, we show here that Wnt3a-induced β-catenin:LEF-1 enhancers recruit cohesin to direct enh...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2015.04.001

    authors： Estarás C,Benner C,Jones KA

    更新日期：2015-06-04 00:00:00

  • Crosstalk between SUMO and ubiquitin on PCNA is mediated by recruitment of the helicase Srs2p.

    abstract：:Posttranslational modification of proliferating cell nuclear antigen (PCNA), an essential processivity clamp for DNA polymerases, by ubiquitin and SUMO contributes to the coordination of DNA replication, damage tolerance, and mutagenesis. Whereas ubiquitination in response to DNA damage promotes the bypass of replicat...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2005.06.001

    authors： Papouli E,Chen S,Davies AA,Huttner D,Krejci L,Sung P,Ulrich HD

    更新日期：2005-07-01 00:00:00

  • Translational control is required for the unfolded protein response and in vivo glucose homeostasis.

    abstract：:The accumulation of unfolded protein in the endoplasmic reticulum (ER) attenuates protein synthesis initiation through phosphorylation of the alpha subunit of eukaryotic translation initiation factor 2 (eIF2alpha) at Ser51. Subsequently, transcription of genes encoding adaptive functions including the glucose-regulate...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(01)00265-9

    authors： Scheuner D,Song B,McEwen E,Liu C,Laybutt R,Gillespie P,Saunders T,Bonner-Weir S,Kaufman RJ

    更新日期：2001-06-01 00:00:00

  • Differential SUMOylation of LXRalpha and LXRbeta mediates transrepression of STAT1 inflammatory signaling in IFN-gamma-stimulated brain astrocytes.

    abstract：:To unravel the roles of LXRs in inflammation and immunity, we examined the function of LXRs in development of IFN-gamma-mediated inflammation using cultured rat brain astrocytes. LXR ligands inhibit neither STAT1 phosphorylation nor STAT1 translocation to the nucleus but, rather, inhibit STAT1 binding to promoters and...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2009.07.021

    authors： Lee JH,Park SM,Kim OS,Lee CS,Woo JH,Park SJ,Joe EH,Jou I

    更新日期：2009-09-24 00:00:00

  • Interleukin 2 receptors and detergent-resistant membrane domains define a clathrin-independent endocytic pathway.

    abstract：:Clathrin-dependent endocytosis has long been presented as the only efficient mechanism by which transmembrane receptors are internalized. We selectively blocked this process using dominant-negative mutants of Eps15 and showed that clathrin-mediated endocytosis of transferrin was inhibited, while endocytosis of interle...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(01)00212-x

    authors： Lamaze C,Dujeancourt A,Baba T,Lo CG,Benmerah A,Dautry-Varsat A

    更新日期：2001-03-01 00:00:00

  • A Conserved Kinase-Based Body-Temperature Sensor Globally Controls Alternative Splicing and Gene Expression.

    abstract：:Homeothermic organisms maintain their core body temperature in a narrow, tightly controlled range. Whether and how subtle circadian oscillations or disease-associated changes in core body temperature are sensed and integrated in gene expression programs remain elusive. Furthermore, a thermo-sensor capable of sensing t...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2020.01.028

    authors： Haltenhof T,Kotte A,De Bortoli F,Schiefer S,Meinke S,Emmerichs AK,Petermann KK,Timmermann B,Imhof P,Franz A,Loll B,Wahl MC,Preußner M,Heyd F

    更新日期：2020-04-02 00:00:00

  • Mitophagy Controls the Activities of Tumor Suppressor p53 to Regulate Hepatic Cancer Stem Cells.

    abstract：:Autophagy is required for benign hepatic tumors to progress into malignant hepatocellular carcinoma. However, the mechanism is unclear. Here, we report that mitophagy, the selective removal of mitochondria by autophagy, positively regulates hepatic cancer stem cells (CSCs) by suppressing the tumor suppressor p53. When...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2017.09.022

    authors： Liu K,Lee J,Kim JY,Wang L,Tian Y,Chan ST,Cho C,Machida K,Chen D,Ou JJ

    更新日期：2017-10-19 00:00:00

  • The mechanism of a neurotransmitter:sodium symporter--inward release of Na+ and substrate is triggered by substrate in a second binding site.

    abstract：:Eukaryotic neurotransmitter:sodium symporters (NSSs), targets for antidepressants and psychostimulants, terminate neurotransmission by sodium-driven reuptake. The crystal structure of LeuT(Aa), a prokaryotic NSS homolog, revealed an occluded state in which one leucine and two Na(+) ions are bound, but provided limited...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2008.05.008

    authors： Shi L,Quick M,Zhao Y,Weinstein H,Javitch JA

    更新日期：2008-06-20 00:00:00

  • Genomic Copy-Number Loss Is Rescued by Self-Limiting Production of DNA Circles.

    abstract：:Copy-number changes generate phenotypic variability in health and disease. Whether organisms protect against copy-number changes is largely unknown. Here, we show that Saccharomyces cerevisiae monitors the copy number of its ribosomal DNA (rDNA) and rapidly responds to copy-number loss with the clonal amplification of...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2018.08.036

    authors： Mansisidor A,Molinar T Jr,Srivastava P,Dartis DD,Pino Delgado A,Blitzblau HG,Klein H,Hochwagen A

    更新日期：2018-11-01 00:00:00

  • Targeting UBE4A Revives Viperin Protein in Epithelium to Enhance Host Antiviral Defense.

    abstract：:Mutation and prevalence of pathogenic viruses prompt the development of broad-spectrum antiviral strategies. Viperin is a potent antiviral protein that inhibits a broad range of viruses. Unexpectedly, we found that Viperin protein production in epithelium is defective in response to both viruses and interferons (IFNs)...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2019.11.003

    authors： Yuan Y,Miao Y,Qian L,Zhang Y,Liu C,Liu J,Zuo Y,Feng Q,Guo T,Zhang L,Chen X,Jin L,Huang F,Zhang H,Zhang W,Li W,Xu G,Zheng H

    更新日期：2020-02-20 00:00:00

  • Metabolism of Free Guanidine in Bacteria Is Regulated by a Widespread Riboswitch Class.

    abstract：:The guanidyl moiety is a component of fundamental metabolites, including the amino acid arginine, the energy carrier creatine, and the nucleobase guanine. Curiously, reports regarding the importance of free guanidine in biology are sparse, and no biological receptors that specifically recognize this compound have been...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2016.11.019

    authors： Nelson JW,Atilho RM,Sherlock ME,Stockbridge RB,Breaker RR

    更新日期：2017-01-19 00:00:00

  • An orphan receptor tyrosine kinase family whose members serve as nonintegrin collagen receptors.

    abstract：:Mammalian cells constantly monitor and respond to a myriad of extracellular signals, often by using cell surface receptors. Two important classes of cell surface receptors include the receptor tyrosine kinases, which recognize peptide growth factors such as insulin, and the integrins, which most often mediate binding ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(00)80004-0

    authors： Shrivastava A,Radziejewski C,Campbell E,Kovac L,McGlynn M,Ryan TE,Davis S,Goldfarb MP,Glass DJ,Lemke G,Yancopoulos GD

    更新日期：1997-12-01 00:00:00

  • Progesterone receptor contains a proline-rich motif that directly interacts with SH3 domains and activates c-Src family tyrosine kinases.

    abstract：:Steroid hormones have rapid nongenomic effects on cell-signaling pathways, but the receptor mechanisms responsible for this are not understood. We have identified a specific polyproline motif in the amino-terminal domain of conventional progesterone receptor (PR) that mediates direct progestin-dependent interaction of...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(01)00304-5

    authors： Boonyaratanakornkit V,Scott MP,Ribon V,Sherman L,Anderson SM,Maller JL,Miller WT,Edwards DP

    更新日期：2001-08-01 00:00:00

  • MCM8- and MCM9-deficient mice reveal gametogenesis defects and genome instability due to impaired homologous recombination.

    abstract：:We generated knockout mice for MCM8 and MCM9 and show that deficiency for these genes impairs homologous recombination (HR)-mediated DNA repair during gametogenesis and somatic cells cycles. MCM8(-/-) mice are sterile because spermatocytes are blocked in meiotic prophase I, and females have only arrested primary folli...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2012.05.048

    authors： Lutzmann M,Grey C,Traver S,Ganier O,Maya-Mendoza A,Ranisavljevic N,Bernex F,Nishiyama A,Montel N,Gavois E,Forichon L,de Massy B,Méchali M

    更新日期：2012-08-24 00:00:00

  • Hsp70- and Hsp90-Mediated Regulation of the Conformation of p53 DNA Binding Domain and p53 Cancer Variants.

    abstract：:The activity of the tumor suppressor p53 has to be timed and balanced closely to prevent untimely induction of cell death. The stability of p53 depends on the ubiquitin ligase Mdm2 but also on Hsp70 and Hsp90 chaperones that interact with its DNA binding domain (DBD). Using hydrogen exchange mass spectrometry and bioc...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2019.03.032

    authors： Boysen M,Kityk R,Mayer MP

    更新日期：2019-05-16 00:00:00

  • Nek1 Regulates Rad54 to Orchestrate Homologous Recombination and Replication Fork Stability.

    abstract：:Never-in-mitosis A-related kinase 1 (Nek1) has established roles in apoptosis and cell cycle regulation. We show that human Nek1 regulates homologous recombination (HR) by phosphorylating Rad54 at Ser572 in late G2 phase. Nek1 deficiency as well as expression of unphosphorylatable Rad54 (Rad54-S572A) cause unresolved ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2016.04.032

    authors： Spies J,Waizenegger A,Barton O,Sürder M,Wright WD,Heyer WD,Löbrich M

    更新日期：2016-06-16 00:00:00

  • The BCL-2 family reunion.

    abstract：:B cell CLL/lymphoma-2 (BCL-2) and its relatives comprise the BCL-2 family of proteins, which were originally characterized with respect to their roles in controlling outer mitochondrial membrane integrity and apoptosis. Current observations expand BCL-2 family function to include numerous cellular pathways. Here we wi...

    journal_title：Molecular cell

    pub_type： 杂志文章,评审

    doi：10.1016/j.molcel.2010.01.025

    authors： Chipuk JE,Moldoveanu T,Llambi F,Parsons MJ,Green DR

    更新日期：2010-02-12 00:00:00

  • Phosphorylation of BRAF by AMPK impairs BRAF-KSR1 association and cell proliferation.

    abstract：:BRAF is an oncogenic protein kinase that drives cell growth and proliferation through the MEK-ERK signaling pathway. BRAF inhibitors have demonstrated antitumor efficacy in melanoma therapy but have also been found to be associated with the development of cutaneous squamous cell carcinomas (cSCCs) in certain patients....

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2013.08.044

    authors： Shen CH,Yuan P,Perez-Lorenzo R,Zhang Y,Lee SX,Ou Y,Asara JM,Cantley LC,Zheng B

    更新日期：2013-10-24 00:00:00

  • Mph1 and Mus81-Mms4 prevent aberrant processing of mitotic recombination intermediates.

    abstract：:Homology-dependent repair of double-strand breaks (DSBs) from nonsister templates has the potential to generate loss of heterozygosity or genome rearrangements. Here we show that the Saccharomyces cerevisiae Mph1 helicase prevents crossovers between ectopic sequences by removing substrates for Mus81-Mms4 or Rad1-Rad10...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2013.09.007

    authors： Mazón G,Symington LS

    更新日期：2013-10-10 00:00:00

  • The Long and the Short of the RNA Polymerase C-Terminal Domain and Phase Separation.

    abstract：:In this issue of Molecular Cell, Lu et al. (2019) analyze the role of the length and sequence complexity of the RNA polymerase II unstructured C-terminal domain in animal viability, development, and the dynamics of RNA polymerase II in vivo. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2019.03.004

    authors： Isaac RS,Churchman LS

    更新日期：2019-03-21 00:00:00

  • Transcriptional ShortCUTs.

    abstract：:Recent work from Kuehner and Brow (2008) and Thiebaut et al. (2008) in Molecular Cell and Jenks et al. (2008) in Molecular and Cellular Biology reveals that regulated expression of central nucleotide synthesis pathway components directs start site-dependent RNA polymerase II termination. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2008.08.014

    authors： Dichtl B

    更新日期：2008-09-05 00:00:00

  • Mechanistic Insights into the cis- and trans-Acting DNase Activities of Cas12a.

    abstract：:CRISPR-Cas12a (Cpf1) is an RNA-guided DNA-cutting nuclease that has been repurposed for genome editing. Upon target DNA binding, Cas12a cleaves both the target DNA in cis and non-target single-stranded DNAs (ssDNAs) in trans. To elucidate the molecular basis for both DNase cleavage modes, we performed structural and b...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2018.11.021

    authors： Swarts DC,Jinek M

    更新日期：2019-02-07 00:00:00

  • MicroRNAs cross the line: the battle for mRNA stability enters the coding sequence.

    abstract：:A study in this issue of Molecular Cell (Elcheva et al., 2009) shows the inherent instability of the betaTrCP1 mRNA to be caused by microRNA-183 targeting the coding sequence; interestingly, this action is directly opposed by the RNA-binding protein CRD-BP. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2009.07.006

    authors： Nielsen AF,Gloggnitzer J,Martinez J

    更新日期：2009-07-31 00:00:00

  • Membrane Protein Dislocation by the Rhomboid Pseudoprotease Dfm1: No Pore Needed?

    abstract：:Defective ER-resident membrane proteins need to be ejected into the cytoplasm in order to be degraded by the proteasome, but the exact mechanism remains unclear. In this issue of Molecular Cell, Neal et al. (2018) reveal that the rhomboid pseudoprotease Dfm1 defines the central ERAD component for membrane protein disl...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2017.12.031

    authors： Avci D,Lemberg MK

    更新日期：2018-01-18 00:00:00

  • MeCP2 binding to DNA depends upon hydration at methyl-CpG.

    abstract：:MeCP2 is an essential transcriptional repressor that mediates gene silencing through binding to methylated DNA. Binding specificity has been thought to depend on hydrophobic interactions between cytosine methyl groups and a hydrophobic patch within the methyl-CpG-binding domain (MBD). X-ray analysis of a methylated DN...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2007.12.028

    authors： Ho KL,McNae IW,Schmiedeberg L,Klose RJ,Bird AP,Walkinshaw MD

    更新日期：2008-02-29 00:00:00

  • Genome and Epigenome Maintenance by Keeping Histone Turnover in Check.

    abstract：:In this issue of Molecular Cell, Taneja et al. (2017) uncover a dual role for the conserved chromatin remodeler Fft3 in the maintenance of silent heterochromatin and the suppression of replication barriers at euchromatic loci through controlled histone turnover. ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2017.03.015

    authors： Fortuny A,Polo SE

    更新日期：2017-04-06 00:00:00