Mechanistic Insights into the cis- and trans-Acting DNase Activities of Cas12a.

abstract：:Stress granules (SGs) are cytoplasmic assemblies of mRNPs stalled in translation initiation. They are induced by various stress conditions, including exposure to the environmental toxin and carcinogen arsenic. While perturbed SG turnover is linked to the pathogenesis of neurodegenerative diseases, the molecular mechan...

journal_title：Molecular cell

authors： Turakhiya A,Meyer SR,Marincola G,Böhm S,Vanselow JT,Schlosser A,Hofmann K,Buchberger A

更新日期：2018-06-07 00:00:00

abstract：:The displacement loop (D loop) is the product of homology search and DNA strand invasion, constituting a central intermediate in homologous recombination (HR). In eukaryotes, the Rad51 DNA strand exchange protein is assisted in D loop formation by the Rad54 motor protein. Curiously, Rad54 also disrupts D loops. How th...

journal_title：Molecular cell

authors： Wright WD,Heyer WD

更新日期：2014-02-06 00:00:00

abstract：:Corticotropin-releasing factor (CRF) and the three related peptides urocortins 1-3 (UCN1-UCN3) are endocrine hormones that control the stress responses by activating CRF1R and CRF2R, two members of class B G-protein-coupled receptors (GPCRs). Here, we present two cryoelectron microscopy (cryo-EM) structures of UCN1-bo...

journal_title：Molecular cell

authors： Ma S,Shen Q,Zhao LH,Mao C,Zhou XE,Shen DD,de Waal PW,Bi P,Li C,Jiang Y,Wang MW,Sexton PM,Wootten D,Melcher K,Zhang Y,Xu HE

更新日期：2020-02-06 00:00:00

abstract：:Perfringolysin O, a bacterial cytolytic toxin, forms unusually large pores in cholesterol-containing membranes by the spontaneous insertion of two of its four domains into the bilayer. By monitoring the kinetics of domain-specific conformational changes and pore formation using fluorescence spectroscopy, the temporal ...

journal_title：Molecular cell

authors： Heuck AP,Hotze EM,Tweten RK,Johnson AE

更新日期：2000-11-01 00:00:00

abstract：:Human cleavage factor I(m) (CFI(m)) is a heterodimeric RNA binding protein complex that functions at an early step in the assembly of the pre-mRNA 3' processing complex. In this report we show that CFI(m) can stimulate both cleavage and poly(A) addition, and can act to suppress poly(A) site cleavage in a sequence-depe...

journal_title：Molecular cell

authors： Brown KM,Gilmartin GM

更新日期：2003-12-01 00:00:00

abstract：:The autophagy cargo receptor p62 facilitates the condensation of misfolded, ubiquitin-positive proteins and their degradation by autophagy, but the molecular mechanism of p62 signaling to the core autophagy machinery is unclear. Here, we show that disordered residues 326-380 of p62 directly interact with the C-termina...

journal_title：Molecular cell

authors： Turco E,Witt M,Abert C,Bock-Bierbaum T,Su MY,Trapannone R,Sztacho M,Danieli A,Shi X,Zaffagnini G,Gamper A,Schuschnig M,Fracchiolla D,Bernklau D,Romanov J,Hartl M,Hurley JH,Daumke O,Martens S

更新日期：2019-04-18 00:00:00

abstract：:APOBEC1 is the catalytic component of an RNA editing complex but shows homology to activation-induced cytidine deaminase (AID), a protein whose function is to potentiate diversification of immunoglobulin gene DNA. Here, we show that APOBEC1 and its homologs APOBEC3C and APOBEC3G exhibit potent DNA mutator activity in ...

journal_title：Molecular cell

authors： Harris RS,Petersen-Mahrt SK,Neuberger MS

更新日期：2002-11-01 00:00:00

abstract：:Epitranscriptomic marks are dynamically placed on mRNA by "writer" or "eraser" enzymes, while "readers" modulate their function accordingly. Lin et al. (2016) now report that the N6-methyladenosine:RNA methyltransferase METTL3 is both a writer and a reader, directly enhancing mRNA translation. ...

journal_title：Molecular cell

authors： Schumann U,Shafik A,Preiss T

更新日期：2016-05-05 00:00:00

abstract：:The biosynthesis of coenzyme Q presents a paradigm for how cells surmount hydrophobic barriers in lipid biology. In eukaryotes, CoQ precursors-among nature's most hydrophobic molecules-must somehow be presented to a series of enzymes peripherally associated with the mitochondrial inner membrane. Here, we reveal that t...

journal_title：Molecular cell

authors： Lohman DC,Aydin D,Von Bank HC,Smith RW,Linke V,Weisenhorn E,McDevitt MT,Hutchins P,Wilkerson EM,Wancewicz B,Russell J,Stefely MS,Beebe ET,Jochem A,Coon JJ,Bingman CA,Dal Peraro M,Pagliarini DJ

更新日期：2019-02-21 00:00:00

abstract：:Microsatellite expansion diseases are caused by unstable tandem repeats of 3-10 nucleotides that become pathogenic beyond a threshold number of copies. Two groups present different approaches to reduce pathogenesis by targeting deactivated Cas9 to either the DNA (Pinto et al., 2017) or the RNA (Batra et al., 2017) rep...

journal_title：Molecular cell

authors： Rao AN,Cooper TA

更新日期：2017-11-02 00:00:00

abstract：:Transcript-selective translational regulation of epithelial-mesenchymal transition (EMT) by transforming growth factor-β (TGF-β) is directed by the hnRNP E1-containing TGF-β-activated-translational (BAT) mRNP complex. Herein, eukaryotic elongation factor-1 A1 (eEF1A1) is identified as an integral component of the BAT ...

journal_title：Molecular cell

authors： Hussey GS,Chaudhury A,Dawson AE,Lindner DJ,Knudsen CR,Wilce MC,Merrick WC,Howe PH

更新日期：2011-02-18 00:00:00

abstract：:Metazoan spliceosomes exhibit an elaborate protein composition required for canonical and alternative splicing. Thus, the minimal set of proteins essential for activation and catalysis remains elusive. We therefore purified in vitro assembled, precatalytic spliceosomal complex B, activated B(act), and step 1 complex C...

journal_title：Molecular cell

authors： Fabrizio P,Dannenberg J,Dube P,Kastner B,Stark H,Urlaub H,Lührmann R

更新日期：2009-11-25 00:00:00

abstract：:The separation of sister chromatids in anaphase depends on the dissociation of cohesin from chromosomes. In vertebrates, some cohesin is removed from chromosomes at the onset of anaphase by proteolytic cleavage. In contrast, the bulk of cohesin is removed from chromosomes already in prophase and prometaphase by an unk...

journal_title：Molecular cell

authors： Sumara I,Vorlaufer E,Stukenberg PT,Kelm O,Redemann N,Nigg EA,Peters JM

更新日期：2002-03-01 00:00:00

abstract：:The CCCH finger protein PIE-1 is a regulator of germ cell fate that segregates with the germ lineage in early embryos. At each asymmetric division, PIE-1 is inherited preferentially by the germline daughter and is excluded from the somatic daughter. We show that this asymmetry is regulated at the protein level by two ...

journal_title：Molecular cell

authors： Reese KJ,Dunn MA,Waddle JA,Seydoux G

更新日期：2000-08-01 00:00:00

abstract：:The Cdc28p cyclin-dependent kinase is thought to both catalyze the onset of DNA replication and prevent rereplication by blocking the reassembly of initiation complexes at replication origins. Budding yeast with mutations in the CDC16 gene represent an exception to this model, because they rereplicate DNA despite bein...

journal_title：Molecular cell

authors： Heichman KA,Roberts JM

更新日期：1998-02-01 00:00:00

abstract：:Takahashi et al. (2018) report that the peptide CLE25 together with the BAM1, BAM3 LRR receptor-like kinases are involved in root-to-shoot communication during dehydration stress in Arabidopsis. ...

journal_title：Molecular cell

authors： McLachlan DH,Pridgeon AJ,Hetherington AM

更新日期：2018-06-21 00:00:00

abstract：:A defining feature of centromeres is the presence of the histone H3 variant CENP-A(Cnp1). It is not known how CENP-A(Cnp1) is specifically delivered to, and assembled into, centromeric chromatin. Through a screen for factors involved in kinetochore integrity in fission yeast, we identified Sim3. Sim3 is homologous to ...

journal_title：Molecular cell

authors： Dunleavy EM,Pidoux AL,Monet M,Bonilla C,Richardson W,Hamilton GL,Ekwall K,McLaughlin PJ,Allshire RC

更新日期：2007-12-28 00:00:00

abstract：:The polycomb repressive complex 2 (PRC2) methylates histones for epigenetic silencing and associates with thousands of protein-coding and noncoding RNAs. How the recruitment of PRC2 to specific sites is facilitated is currently unclear. Two recent studies have deciphered the impact of RNA binding on PRC2 recruitment a...

journal_title：Molecular cell

authors： Kretz M,Meister G

更新日期：2014-07-17 00:00:00

abstract：:The DNA polymerase from phage phi29 is a B family polymerase that initiates replication using a protein as a primer, attaching the first nucleotide of the phage genome to the hydroxyl of a specific serine of the priming protein. The crystal structure of phi29 DNA polymerase determined at 2.2 A resolution provides expl...

journal_title：Molecular cell

authors： Kamtekar S,Berman AJ,Wang J,Lázaro JM,de Vega M,Blanco L,Salas M,Steitz TA

更新日期：2004-11-19 00:00:00

abstract：:The translocon is the gateway to the endoplasmic reticulum (ER). In yeast this is the Sec61p complex. However, new evidence suggests that a second translocon containing the Sec61p homolog Ssh1p provides important flexibility to the ER translocation machinery. ...

journal_title：Molecular cell

authors： Robb A,Brown JD

更新日期：2001-09-01 00:00:00

abstract：:Plant pattern recognition receptors (PRRs) perceive microbial and endogenous molecular patterns to activate immune signaling. The cytoplasmic kinase BIK1 acts downstream of multiple PRRs as a rate-limiting component, whose phosphorylation and accumulation are central to immune signal propagation. Previous work identif...

journal_title：Molecular cell

authors： Wang J,Grubb LE,Wang J,Liang X,Li L,Gao C,Ma M,Feng F,Li M,Li L,Zhang X,Yu F,Xie Q,Chen S,Zipfel C,Monaghan J,Zhou JM

更新日期：2018-02-01 00:00:00

abstract：:Upon recruitment to active enhancers and promoters, RNA polymerase II (Pol II) generates short non-coding transcripts of unclear function. The mechanisms that control the length and the amount of ncRNAs generated by cis-regulatory elements are largely unknown. Here, we show that the adaptor protein WDR82 and its assoc...

journal_title：Molecular cell

authors： Austenaa LM,Barozzi I,Simonatto M,Masella S,Della Chiara G,Ghisletti S,Curina A,de Wit E,Bouwman BA,de Pretis S,Piccolo V,Termanini A,Prosperini E,Pelizzola M,de Laat W,Natoli G

更新日期：2015-11-05 00:00:00

abstract：:Genome instability, a hallmark of cancer progression, is thought to arise through DNA double strand breaks (DSBs). Studies in yeast and mammalian cells have shown that DSBs and instability can occur through RNA:DNA hybrids generated by defects in RNA elongation and splicing. We report that in yeast hybrids naturally f...

journal_title：Molecular cell

authors： Wahba L,Amon JD,Koshland D,Vuica-Ross M

更新日期：2011-12-23 00:00:00

abstract：:The RNA-binding protein HuR, while known to stabilize cytoplasmic mRNAs, is largely nuclear. In this issue of Molecular Cell, Mukherjee et al. (2011) and Lebedeva et al. (2011) identify transcriptome-wide HuR-RNA interactions using PAR-CLIP, unveiling HuR's nuclear role in pre-mRNA processing. ...

journal_title：Molecular cell

authors： Srikantan S,Gorospe M

更新日期：2011-08-05 00:00:00

abstract：:Polycomb group (PcG) proteins exert essential functions in the most disparate biological processes. The contribution of PcG proteins to cell commitment and differentiation relates to their ability to repress transcription of developmental regulators in embryonic stem (ES) cells and in committed cell lineages, includin...

journal_title：Molecular cell

authors： Juan AH,Kumar RM,Marx JG,Young RA,Sartorelli V

更新日期：2009-10-09 00:00:00

abstract：:Death-fold domains constitute an evolutionarily conserved superfamily that mediates apoptotic signaling. These motifs, including CARD (caspase recruitment domain), DD (death domain), and DED (death effector domain), are believed to exert their effects solely through homotypic interactions. Herein we demonstrate that t...

journal_title：Molecular cell

authors： Nam YJ,Mani K,Ashton AW,Peng CF,Krishnamurthy B,Hayakawa Y,Lee P,Korsmeyer SJ,Kitsis RN

更新日期：2004-09-24 00:00:00

abstract：:Tumor progression shares many characteristics with the process of epithelial-to-mesenchymal transition (EMT). Cells that have undergone an EMT are known to have an increased resistance to apoptosis. CD95/Fas is an apoptosis-inducing receptor expressed on many tissues and tumor cells. During tumor progression CD95 is f...

journal_title：Molecular cell

authors： Schickel R,Park SM,Murmann AE,Peter ME

更新日期：2010-06-25 00:00:00

abstract：:The intracellular regulation of cell death pathways by cIAPs has been enigmatic. Here we show that loss of cIAPs promotes the spontaneous formation of an intracellular platform that activates either apoptosis or necroptosis. This 2 MDa intracellular complex that we designate "Ripoptosome" is necessary but not sufficie...

journal_title：Molecular cell

authors： Feoktistova M,Geserick P,Kellert B,Dimitrova DP,Langlais C,Hupe M,Cain K,MacFarlane M,Häcker G,Leverkus M

更新日期：2011-08-05 00:00:00

abstract：:The nucleotide (p)ppGpp mediates bacterial stress responses, but its targets and underlying mechanisms of action vary among bacterial species and remain incompletely understood. Here, we characterize the molecular interaction between (p)ppGpp and guanylate kinase (GMK), revealing the importance of this interaction in ...

journal_title：Molecular cell

authors： Liu K,Myers AR,Pisithkul T,Claas KR,Satyshur KA,Amador-Noguez D,Keck JL,Wang JD

更新日期：2015-02-19 00:00:00

abstract：:How eukaryotic chromosomes fold inside the nucleus is an age-old question that remains unanswered today. Early biochemical and microscopic studies revealed the existence of chromatin domains and loops as a pervasive feature of interphase chromosomes, but the biological implications of such organizational features were...

journal_title：Molecular cell

authors： Dixon JR,Gorkin DU,Ren B

更新日期：2016-06-02 00:00:00