Structural Basis of the Differential Function of the Two C. elegans Atg8 Homologs, LGG-1 and LGG-2, in Autophagy.

abstract：:We used computational algorithms to find conserved sequences in the 3' untranslated region (UTR) of transcripts that exhibited rapid decay in primary human T cells and found that the consensus sequence UGUUUGUUUGU, which we have termed a GU-rich element (GRE), was enriched in short-lived transcripts. Using a tet-off r...

journal_title：Molecular cell

authors： Vlasova IA,Tahoe NM,Fan D,Larsson O,Rattenbacher B,Sternjohn JR,Vasdewani J,Karypis G,Reilly CS,Bitterman PB,Bohjanen PR

更新日期：2008-02-01 00:00:00

abstract：:Human cleavage factor I(m) (CFI(m)) is a heterodimeric RNA binding protein complex that functions at an early step in the assembly of the pre-mRNA 3' processing complex. In this report we show that CFI(m) can stimulate both cleavage and poly(A) addition, and can act to suppress poly(A) site cleavage in a sequence-depe...

journal_title：Molecular cell

authors： Brown KM,Gilmartin GM

更新日期：2003-12-01 00:00:00

abstract：:The majority of the human genome is transcribed into non-coding (nc)RNAs that lack known biological functions or else are only partially characterized. Numerous characterized ncRNAs function via base pairing with target RNA sequences to direct their biological activities, which include critical roles in RNA processing...

journal_title：Molecular cell

authors： Sharma E,Sterne-Weiler T,O'Hanlon D,Blencowe BJ

更新日期：2016-05-19 00:00:00

abstract：:Faithful propagation of specific chromatin states requires re-establishment of epigenetic marks after every cell division. How the original epigenetic signature is inherited after disruption during DNA replication is still poorly understood. Here, we show that the poly(ADP-ribose)-polymerase-1 (PARP1/ARTD1) is implica...

journal_title：Molecular cell

authors： Guetg C,Scheifele F,Rosenthal F,Hottiger MO,Santoro R

更新日期：2012-03-30 00:00:00

abstract：:During meiosis, recombination between homologous chromosomes generates crossover (CR) and noncrossover (NCR) products. CRs establish connections between homologs, whereas intermediates leading to NCRs have been proposed to participate in homologous pairing. How these events are differentiated and regulated remains to ...

journal_title：Molecular cell

authors： Guillon H,Baudat F,Grey C,Liskay RM,de Massy B

更新日期：2005-11-23 00:00:00

abstract：:Using genome-wide approaches, we have elucidated the regulatory circuitry governed by the XBP1 transcription factor, a key effector of the mammalian unfolded protein response (UPR), in skeletal muscle and secretory cells. We identified a core group of genes involved in constitutive maintenance of ER function in all ce...

journal_title：Molecular cell

authors： Acosta-Alvear D,Zhou Y,Blais A,Tsikitis M,Lents NH,Arias C,Lennon CJ,Kluger Y,Dynlacht BD

更新日期：2007-07-06 00:00:00

abstract：:An exciting study in this issue reveals how binding of the microtubule associated protein TPX2 to the mitotic kinase Aurora A induces a conformational change. This moves the phosphorylated activation domain into a more compact position within the kinase core, providing a better substrate binding platform and hiding th...

journal_title：Molecular cell

authors： Glover DM

更新日期：2003-10-01 00:00:00

abstract：:Multisite phosphorylation of kinases can induce on-off or graded regulation of catalytic activity; however, its influence on substrate specificity remains unclear. Here, we show that multisite phosphorylation of ribosomal protein S6 kinase 1 (S6K1) alters target selection. Agonist-inducible phosphorylation of glutamyl...

journal_title：Molecular cell

authors： Arif A,Jia J,Willard B,Li X,Fox PL

更新日期：2019-02-07 00:00:00

abstract：:Argonaute proteins are the mediators of small RNA-guided gene silencing pathways. In this issue, Couvillion and coworkers (Couvillion et al., 2012) found an unexpected function for a Tetrahymena Argonaute protein: It forms a complex with tRNA fragments and is required for nuclear Xrn2 localization and function. ...

journal_title：Molecular cell

authors： Hasler D,Meister G

更新日期：2012-11-30 00:00:00

abstract：:DNA replication introduces a dosage imbalance between early and late replicating genes. In budding yeast, buffering gene expression against this imbalance depends on marking replicated DNA by H3K56 acetylation (H3K56ac). Whether additional processes are required for suppressing transcription from H3K56ac-labeled DNA r...

journal_title：Molecular cell

authors： Voichek Y,Mittelman K,Gordon Y,Bar-Ziv R,Lifshitz Smit D,Shenhav R,Barkai N

更新日期：2018-06-21 00:00:00

abstract：:There are many regulated steps in the assembly of a transcription preinitiation complex (PIC). In this issue of Molecular Cell, Black et. al. (2006) reveal a catalytic switch mechanism in which autoacetylation of the HAT p300 triggers its dissociation from a promoter in a manner that is coupled to TFIID association. ...

journal_title：Molecular cell

authors： Pugh BF

更新日期：2006-09-15 00:00:00

abstract：:The accurate propagation of histone marks during chromosomal replication is proposed to rely on the tight coupling of replication with the recycling of parental histones to the daughter strands. Here, we show in the avian cell line DT40 that REV1, a key regulator of DNA translesion synthesis at the replication fork, i...

journal_title：Molecular cell

authors： Sarkies P,Reams C,Simpson LJ,Sale JE

更新日期：2010-12-10 00:00:00

abstract：:SOCS1 is lost in many human tumors, but its tumor suppression activities are not well understood. We report that SOCS1 is required for transcriptional activity, DNA binding, and serine 15 phosphorylation of p53 in the context of STAT5 signaling. In agreement, inactivation of SOCS1 disabled p53-dependent senescence in ...

journal_title：Molecular cell

authors： Calabrese V,Mallette FA,Deschênes-Simard X,Ramanathan S,Gagnon J,Moores A,Ilangumaran S,Ferbeyre G

更新日期：2009-12-11 00:00:00

abstract：:The stress response in eukaryotic cells often inhibits translation initiation and leads to the formation of cytoplasmic RNA-protein complexes referred to as stress granules. Stress granules contain nontranslating mRNAs, translation initiation components, and many additional proteins affecting mRNA function. Stress gra...

journal_title：Molecular cell

authors： Buchan JR,Parker R

更新日期：2009-12-25 00:00:00

abstract：:The tumor suppressor p53 transcriptionally activates target genes to suppress cellular proliferation during stress. p53 has also been implicated in the repression of the proto-oncogene Myc, but the mechanism has remained unclear. Here, we identify Pvt1b, a p53-dependent isoform of the long noncoding RNA (lncRNA) Pvt1,...

journal_title：Molecular cell

authors： Olivero CE,Martínez-Terroba E,Zimmer J,Liao C,Tesfaye E,Hooshdaran N,Schofield JA,Bendor J,Fang D,Simon MD,Zamudio JR,Dimitrova N

更新日期：2020-02-20 00:00:00

abstract：:Folding-defective proteins must be cleared efficiently from the endoplasmic reticulum (ER) to prevent perturbation of the folding environment and to maintain cellular proteostasis. Misfolded proteins engage dislocation machineries (dislocons) built around E3 ubiquitin ligases that promote their transport across the ER...

journal_title：Molecular cell

authors： Bernasconi R,Galli C,Kokame K,Molinari M

更新日期：2013-12-26 00:00:00

abstract：:Perfringolysin O, a bacterial cytolytic toxin, forms unusually large pores in cholesterol-containing membranes by the spontaneous insertion of two of its four domains into the bilayer. By monitoring the kinetics of domain-specific conformational changes and pore formation using fluorescence spectroscopy, the temporal ...

journal_title：Molecular cell

authors： Heuck AP,Hotze EM,Tweten RK,Johnson AE

更新日期：2000-11-01 00:00:00

abstract：:Rapid recognition and signal transduction of mechanical wounding through various signaling molecules, including calcium (Ca²+), protein phosphorylation, and reactive oxygen species (ROS), are necessary early events leading to stress resistance in plants. Here we report that an Arabidopsis mitogen-activated protein kin...

journal_title：Molecular cell

authors： Takahashi F,Mizoguchi T,Yoshida R,Ichimura K,Shinozaki K

更新日期：2011-03-18 00:00:00

abstract：:The RNA modification N6-methyladenosine (m6A) modulates mRNA fate and thus affects many biological processes. We analyzed m6A across the transcriptome following infection by dengue virus (DENV), Zika virus (ZIKV), West Nile virus (WNV), and hepatitis C virus (HCV). We found that infection by these viruses in the Flavi...

journal_title：Molecular cell

authors： Gokhale NS,McIntyre ABR,Mattocks MD,Holley CL,Lazear HM,Mason CE,Horner SM

更新日期：2020-02-06 00:00:00

abstract：:Argonaute proteins are required for the biogenesis of some small RNAs (sRNAs), including the PIWI-interacting RNAs and some microRNAs. How Argonautes mediate maturation of sRNAs independent of their slicer activity is not clear. The maturation of the Neurospora microRNA-like sRNA, milR-1, requires the Argonaute protei...

journal_title：Molecular cell

authors： Xue Z,Yuan H,Guo J,Liu Y

更新日期：2012-05-11 00:00:00

abstract：:Activation of NF-kappaB is regulated via numerous ubiquitin- and proteasome-mediated steps; an important one is processing of the precursor p105 to the p50 active subunit. The mechanisms involved are largely unknown, because this is an exceptional case where the ubiquitin system does not destroy its substrate complete...

journal_title：Molecular cell

authors： Kravtsova-Ivantsiv Y,Cohen S,Ciechanover A

更新日期：2009-02-27 00:00:00

abstract：:Genome instability, a hallmark of cancer progression, is thought to arise through DNA double strand breaks (DSBs). Studies in yeast and mammalian cells have shown that DSBs and instability can occur through RNA:DNA hybrids generated by defects in RNA elongation and splicing. We report that in yeast hybrids naturally f...

journal_title：Molecular cell

authors： Wahba L,Amon JD,Koshland D,Vuica-Ross M

更新日期：2011-12-23 00:00:00

abstract：:Both splicing factors and microRNAs are important regulatory molecules that play key roles in posttranscriptional gene regulation. By miRNA deep sequencing, we identified 40 miRNAs that are differentially expressed upon ectopic overexpression of the splicing factor SF2/ASF. Here we show that SF2/ASF and one of its upr...

journal_title：Molecular cell

authors： Wu H,Sun S,Tu K,Gao Y,Xie B,Krainer AR,Zhu J

更新日期：2010-04-09 00:00:00

abstract：:In this issue of Molecular Cell, Miotto and Struhl (2010) suggest that replication licensing, the loading of Mcm2-7 onto DNA, is promoted by HBO1 acetylating histone H4 at replication origins, providing a molecular view of how chromatin status influences origin usage. ...

journal_title：Molecular cell

authors： Chadha GS,Blow JJ

更新日期：2010-01-15 00:00:00

abstract：:In vitro, the anaphase-promoting complex (APC) E3 ligase functions with E2 ubiquitin-conjugating enzymes of the E2-C and Ubc4/5 families to ubiquitinate substrates. However, only the use of the E2-C family, notably UbcH10, is genetically well validated. Here, we biochemically demonstrate preferential use of UbcH10 by ...

journal_title：Molecular cell

authors： Summers MK,Pan B,Mukhyala K,Jackson PK

更新日期：2008-08-22 00:00:00

abstract：:Gene expression mediated by viral vectors is subject to cell-to-cell variability, which limits the accuracy of gene delivery. When coupled with single-cell measurements, however, such variability provides an efficient means to quantify signaling dynamics in mammalian cells. Here, we illustrate the utility of this appr...

journal_title：Molecular cell

authors： Wong JV,Yao G,Nevins JR,You L

更新日期：2011-02-04 00:00:00

abstract：:Nuclear RNA interference (RNAi) pathways work together with histone modifications to regulate gene expression and enact an adaptive response to transposable RNA elements. In the germline, nuclear RNAi can lead to trans-generational epigenetic inheritance (TEI) of gene silencing. We identified and characterized a famil...

journal_title：Molecular cell

authors： Lewis A,Berkyurek AC,Greiner A,Sawh AN,Vashisht A,Merrett S,Flamand MN,Wohlschlegel J,Sarov M,Miska EA,Duchaine TF

更新日期：2020-06-04 00:00:00

abstract：:Fluoxetine (Prozac) is an antidepressant that is thought to act by blocking presynaptic reuptake of the neurotransmitter serotonin. Despite widespread clinical use of fluoxetine, direct evidence for this mechanism has been difficult to obtain in vivo. We have determined that fluoxetine has an additional neuromuscular ...

journal_title：Molecular cell

authors： Choy RK,Thomas JH

更新日期：1999-08-01 00:00:00

abstract：:Circular RNAs (circRNAs) are abundant and evolutionarily conserved RNAs of largely unknown function. Here, we show that a subset of circRNAs is translated in vivo. By performing ribosome footprinting from fly heads, we demonstrate that a group of circRNAs is associated with translating ribosomes. Many of these ribo-ci...

journal_title：Molecular cell

authors： Pamudurti NR,Bartok O,Jens M,Ashwal-Fluss R,Stottmeister C,Ruhe L,Hanan M,Wyler E,Perez-Hernandez D,Ramberger E,Shenzis S,Samson M,Dittmar G,Landthaler M,Chekulaeva M,Rajewsky N,Kadener S

更新日期：2017-04-06 00:00:00

abstract：:Checkpoint proteins respond to DNA damage by halting the cell cycle until the damage is repaired. In this issue of Molecular Cell, Furuya et al. (2010) provide evidence that checkpoint proteins need to be removed from sites of damage in order to properly repair it. ...

journal_title：Molecular cell

authors： Paek AL,Weinert T

更新日期：2010-11-24 00:00:00