Abstract:
:Communication between the 5' cap structure and 3' poly(A) tail of eukaryotic mRNA results in the synergistic enhancement of translation. The cap and poly(A) tail binding proteins, eIF4E and Pab1p, mediate this effect in the yeast S. cerevisiae through their interactions with different parts of the translation factor eIF4G. Here, we demonstrate the reconstitution of an eIF4E/eIF4G/Pab1p complex with recombinant proteins, and show by atomic force microscopy that the complex can circularize capped, polyadenylated RNA. Our results suggest that formation of circular mRNA by translation factors could contribute to the control of mRNA expression in the eukaryotic cell.
journal_name
Mol Celljournal_title
Molecular cellauthors
Wells SE,Hillner PE,Vale RD,Sachs ABdoi
10.1016/s1097-2765(00)80122-7subject
Has Abstractpub_date
1998-07-01 00:00:00pages
135-40issue
1eissn
1097-2765issn
1097-4164pii
S1097-2765(00)80122-7journal_volume
2pub_type
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