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Gcn4 Binding in Coding Regions Can Activate Internal and Canonical 5' Promoters in Yeast.

Abstract：

:Gcn4 is a yeast transcriptional activator induced by amino acid starvation. ChIP-seq analysis revealed 546 genomic sites occupied by Gcn4 in starved cells, representing ∼30% of Gcn4-binding motifs. Surprisingly, only ∼40% of the bound sites are in promoters, of which only ∼60% activate transcription, indicating extensive negative control over Gcn4 function. Most of the remaining ∼300 Gcn4-bound sites are within coding sequences (CDSs), with ∼75 representing the only bound sites near Gcn4-induced genes. Many such unconventional sites map between divergent antisense and sub-genic sense transcripts induced within CDSs adjacent to induced TBP peaks, consistent with Gcn4 activation of cryptic bidirectional internal promoters. Mutational analysis confirms that Gcn4 sites within CDSs can activate sub-genic and full-length transcripts from the same or adjacent genes, showing that functional Gcn4 binding is not confined to promoters. Our results show that internal promoters can be regulated by an activator that functions at conventional 5'-positioned promoters.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Rawal Y,Chereji RV,Valabhoju V,Qiu H,Ocampo J,Clark DJ,Hinnebusch AG

doi

10.1016/j.molcel.2018.03.007

subject

Has Abstract

pub_date

2018-04-19 00:00:00

pages

297-311.e4

issue

2

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(18)30188-6

journal_volume

70

pub_type

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