A recombinant RNA polymerase II-like enzyme capable of promoter-specific transcription.

Abstract:

:RNA polymerases (RNAPs) are core components of the cellular transcriptional machinery. Progress with functional studies of eukaryotic RNAPs has been delayed by the fact that it has not yet been possible to assemble active enzymes from individual subunits. Archaeal RNAPs are directly comparable to eukaryotic RNAPII in terms of primary sequence homology and quaternary structure. Here we report the successful in vitro assembly of a recombinant archaeal RNAP from purified subunits. The recombinant enzyme displays full activity in transcription assays and is capable, in the presence of two other basal factors, of promoter-specific transcription. The assembly of mutant enzymes yielded several unexpected insights into the structural and functional contributions of various subunits toward overall RNAP activity.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Werner F,Weinzierl RO

doi

10.1016/s1097-2765(02)00629-9

subject

Has Abstract

pub_date

2002-09-01 00:00:00

pages

635-46

issue

3

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(02)00629-9

journal_volume

10

pub_type

杂志文章
  • Insights into FlaI functions in archaeal motor assembly and motility from structures, conformations, and genetics.

    abstract::Superfamily ATPases in type IV pili, type 2 secretion, and archaella (formerly archaeal flagella) employ similar sequences for distinct biological processes. Here, we structurally and functionally characterize prototypical superfamily ATPase FlaI in Sulfolobus acidocaldarius, showing FlaI activities in archaeal swimmi...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2013.01.014

    authors: Reindl S,Ghosh A,Williams GJ,Lassak K,Neiner T,Henche AL,Albers SV,Tainer JA

    更新日期:2013-03-28 00:00:00

  • Too sweet for autophagy: hexokinase inhibition of mTORC1 activates autophagy.

    abstract::In this issue, Roberts et al. (2014) describe how hexokinase and mTORC1 cooperate to sense disequilibrium between glucose uptake and utilization and direct the balance of anabolism and catabolism to ensure the appropriate use of cellular resources. ...

    journal_title:Molecular cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.molcel.2014.02.009

    authors: Kundu M

    更新日期:2014-02-20 00:00:00

  • NOTCH1 nuclear interactome reveals key regulators of its transcriptional activity and oncogenic function.

    abstract::Activating mutations in NOTCH1, an essential regulator of T cell development, are frequently found in human T cell acute lymphoblastic leukemia (T-ALL). Despite important advances in our understanding of Notch signal transduction, the regulation of Notch functions in the nucleus remains unclear. Using immunoaffinity p...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2012.08.022

    authors: Yatim A,Benne C,Sobhian B,Laurent-Chabalier S,Deas O,Judde JG,Lelievre JD,Levy Y,Benkirane M

    更新日期:2012-11-09 00:00:00

  • MitoCPR: Meticulous Monitoring of Mitochondrial Proteostasis.

    abstract::Mitochondrial protein import stress compromises functioning of the organelles, due to inadequate supply of inner mitochondrial proteins. Weidberg and Amon (2018) describe a new monitoring pathway in budding yeast, which restores mitochondrial function following the clearing of accumulated unfolded pre-transported mito...

    journal_title:Molecular cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.molcel.2018.06.027

    authors: Desai R,Campanella M

    更新日期:2018-07-05 00:00:00

  • Partners in Crime: Clandestine Operations among RAS-RAF Accomplices in Promoting Tumorigenesis.

    abstract::In this issue of Molecular Cell, Terrell et al. (2019) characterize the interactions of RAS-RAF complexes in live cells. Using bioluminescence resonance energy transfer (BRET) technology, they show that individual RAF family members exhibit distinct binding affinities to each RAS family protein, with CRAF exhibiting h...

    journal_title:Molecular cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.molcel.2019.11.020

    authors: Venkatanarayan A,Malek S

    更新日期:2019-12-19 00:00:00

  • Meiotic recombination remote from prominent DNA break sites in S. pombe.

    abstract::DNA breakage is intimately associated with meiotic recombination in the fission yeast Schizosaccharomyces pombe. Sites of prominent DNA breakage were found approximately 25 to approximately 200 kb apart in the genomic regions surveyed. We examined in detail a 501 kb region of chromosome I and found six sites, or tight...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/s1097-2765(02)00452-5

    authors: Young JA,Schreckhise RW,Steiner WW,Smith GR

    更新日期:2002-02-01 00:00:00

  • Acetylation Enhances TET2 Function in Protecting against Abnormal DNA Methylation during Oxidative Stress.

    abstract::TET proteins, by converting 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), are hypothesized, but not directly shown, to protect promoter CpG islands (CGIs) against abnormal DNA methylation (DNAm) in cancer. We define such a protective role linked to DNA damage from oxidative stress (OS) known to induce this...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2016.12.013

    authors: Zhang YW,Wang Z,Xie W,Cai Y,Xia L,Easwaran H,Luo J,Yen RC,Li Y,Baylin SB

    更新日期:2017-01-19 00:00:00

  • The Mitochondrial Basis of Aging.

    abstract::A decline in mitochondrial quality and activity has been associated with normal aging and correlated with the development of a wide range of age-related diseases. Here, we review the evidence that a decline in mitochondria function contributes to aging. In particular, we discuss how mitochondria contribute to specific...

    journal_title:Molecular cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.molcel.2016.01.028

    authors: Sun N,Youle RJ,Finkel T

    更新日期:2016-03-03 00:00:00

  • The crystal structure and mode of action of trans-sialidase, a key enzyme in Trypanosoma cruzi pathogenesis.

    abstract::Trans-sialidases (TS) are GPI-anchored surface enzymes expressed in specific developmental stages of trypanosome parasites like Trypanosoma cruzi, the etiologic agent of Chagas disease, and T. brucei, the causative agent of sleeping sickness. TS catalyzes the transfer of sialic acid residues from host to parasite glyc...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/s1097-2765(02)00680-9

    authors: Buschiazzo A,Amaya MF,Cremona ML,Frasch AC,Alzari PM

    更新日期:2002-10-01 00:00:00

  • A cathepsin L isoform that is devoid of a signal peptide localizes to the nucleus in S phase and processes the CDP/Cux transcription factor.

    abstract::The subclass of cysteine proteases termed lysosomal cathepsins has long been thought to be primarily involved in end-stage protein breakdown within lysosomal compartments. Furthermore, few specific protein substrates for these proteases have been identified. We show here that cathepsin L functions in the regulation of...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/s1097-2765(04)00209-6

    authors: Goulet B,Baruch A,Moon NS,Poirier M,Sansregret LL,Erickson A,Bogyo M,Nepveu A

    更新日期:2004-04-23 00:00:00

  • Crystal structure of Hck in complex with a Src family-selective tyrosine kinase inhibitor.

    abstract::The crystal structure of the autoinhibited form of Hck has been determined at 2.0 A resolution, in complex with a specific pyrazolo pyrimidine-type inhibitor, PP1. The activation segment, a key regulatory component of the catalytic domain, is unphosphorylated and is visualized in its entirety. Tyr-416, the site of act...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/s1097-2765(00)80357-3

    authors: Schindler T,Sicheri F,Pico A,Gazit A,Levitzki A,Kuriyan J

    更新日期:1999-05-01 00:00:00

  • Targeting UBE4A Revives Viperin Protein in Epithelium to Enhance Host Antiviral Defense.

    abstract::Mutation and prevalence of pathogenic viruses prompt the development of broad-spectrum antiviral strategies. Viperin is a potent antiviral protein that inhibits a broad range of viruses. Unexpectedly, we found that Viperin protein production in epithelium is defective in response to both viruses and interferons (IFNs)...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2019.11.003

    authors: Yuan Y,Miao Y,Qian L,Zhang Y,Liu C,Liu J,Zuo Y,Feng Q,Guo T,Zhang L,Chen X,Jin L,Huang F,Zhang H,Zhang W,Li W,Xu G,Zheng H

    更新日期:2020-02-20 00:00:00

  • Multimodal activation of the ubiquitin ligase SCF by Nedd8 conjugation.

    abstract::Conjugation of ubiquitin-like protein Nedd8 to cullins (neddylation) is essential for the function of cullin-RING ubiquitin ligases (CRLs). Here, we show that neddylation stimulates CRL activity by multiple mechanisms. For the initiator ubiquitin, the major effect is to bridge the approximately 50 A gap between naked ...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2008.08.021

    authors: Saha A,Deshaies RJ

    更新日期:2008-10-10 00:00:00

  • A switch in mitotic histone H4 lysine 20 methylation status is linked to M phase defects upon loss of HCF-1.

    abstract::The abundant chromatin-associated human factor HCF-1 is a heterodimeric complex of HCF-1N and HCF-1C subunits that are essential for two stages of the cell cycle. The HCF-1N subunit promotes G1 phase progression, whereas the HCF-1C subunit ensures proper cytokinesis at completion of M phase. How the HCF-1C subunit fun...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2004.06.008

    authors: Julien E,Herr W

    更新日期:2004-06-18 00:00:00

  • The Chaperone Activity of the Developmental Small Heat Shock Protein Sip1 Is Regulated by pH-Dependent Conformational Changes.

    abstract::Small heat shock proteins (sHsps) are ubiquitous molecular chaperones that prevent the aggregation of unfolding proteins during proteotoxic stress. In Caenorhabditis elegans, Sip1 is the only sHsp exclusively expressed in oocytes and embryos. Here, we demonstrate that Sip1 is essential for heat shock survival of repro...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2015.04.019

    authors: Fleckenstein T,Kastenmüller A,Stein ML,Peters C,Daake M,Krause M,Weinfurtner D,Haslbeck M,Weinkauf S,Groll M,Buchner J

    更新日期:2015-06-18 00:00:00

  • Pin1At encoding a peptidyl-prolyl cis/trans isomerase regulates flowering time in Arabidopsis.

    abstract::Floral transition in plants is regulated by an integrated network of flowering genetic pathways. We show that an Arabidopsis PIN1-type parvulin 1, Pin1At, controls floral transition by accelerating cis/trans isomerization of the phosphorylated Ser/Thr-Pro motifs in two MADS-domain transcription factors, SUPPRESSOR OF ...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2009.12.020

    authors: Wang Y,Liu C,Yang D,Yu H,Liou YC

    更新日期:2010-01-15 00:00:00

  • EGFR-Phosphorylated Platelet Isoform of Phosphofructokinase 1 Promotes PI3K Activation.

    abstract::EGFR activates phosphatidylinositide 3-kinase (PI3K), but the mechanism underlying this activation is not completely understood. We demonstrated here that EGFR activation resulted in lysine acetyltransferase 5 (KAT5)-mediated K395 acetylation of the platelet isoform of phosphofructokinase 1 (PFKP) and subsequent trans...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2018.03.018

    authors: Lee JH,Liu R,Li J,Wang Y,Tan L,Li XJ,Qian X,Zhang C,Xia Y,Xu D,Guo W,Ding Z,Du L,Zheng Y,Chen Q,Lorenzi PL,Mills GB,Jiang T,Lu Z

    更新日期:2018-04-19 00:00:00

  • A Multiplexed Assay for Exon Recognition Reveals that an Unappreciated Fraction of Rare Genetic Variants Cause Large-Effect Splicing Disruptions.

    abstract::Mutations that lead to splicing defects can have severe consequences on gene function and cause disease. Here, we explore how human genetic variation affects exon recognition by developing a multiplexed functional assay of splicing using Sort-seq (MFASS). We assayed 27,733 variants in the Exome Aggregation Consortium ...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2018.10.037

    authors: Cheung R,Insigne KD,Yao D,Burghard CP,Wang J,Hsiao YE,Jones EM,Goodman DB,Xiao X,Kosuri S

    更新日期:2019-01-03 00:00:00

  • The N-terminal domain of Nup159 forms a beta-propeller that functions in mRNA export by tethering the helicase Dbp5 to the nuclear pore.

    abstract::Nuclear export of mRNA in eukaryotic cells is mediated by soluble transport factors and components of the nuclear pore complex (NPC). The cytoplasmically oriented nuclear pore protein Nup159 plays a critical role in mRNA export through its conserved N-terminal domain (NTD). Here, we report the crystal structure of the...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2004.10.032

    authors: Weirich CS,Erzberger JP,Berger JM,Weis K

    更新日期:2004-12-03 00:00:00

  • A pathway of sequential arginine-serine-rich domain-splicing signal interactions during mammalian spliceosome assembly.

    abstract::Serine-arginine (SR) proteins are general splicing factors and can function through binding to exonic splicing enhancers (ESEs). SR proteins and several other mammalian splicing factors contain an arginine-serine-rich (RS) domain required to promote splicing. We have recently found that the ESE bound RS domain functio...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2004.10.021

    authors: Shen H,Green MR

    更新日期:2004-11-05 00:00:00

  • Ribosome structure and activity are altered in cells lacking snoRNPs that form pseudouridines in the peptidyl transferase center.

    abstract::One of the oldest questions in RNA science is the role of nucleotide modification. Here, the importance of pseudouridine formation (Psi) in the peptidyl transferase center of rRNA was examined by depleting yeast cells of 1-5 snoRNAs that guide a total of six Psi modifications. Translation was impaired substantially wi...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/s1097-2765(03)00040-6

    authors: King TH,Liu B,McCully RR,Fournier MJ

    更新日期:2003-02-01 00:00:00

  • SOSS1/2: Sensors of single-stranded DNA at a break.

    abstract::In this issue of Molecular Cell, Huang et al. (2009) describe two heterotrimeric single-stranded DNA binding complexes, SOSS1 and SOSS2, that function downstream of the MRN complex to promote DNA repair and the G2/M checkpoint. ...

    journal_title:Molecular cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.molcel.2009.07.016

    authors: Nam EA,Cortez D

    更新日期:2009-08-14 00:00:00

  • Imaging interorganelle contacts and local calcium dynamics at the ER-mitochondrial interface.

    abstract::The ER-mitochondrial junction provides a local calcium signaling domain that is critical for both matching energy production with demand and the control of apoptosis. Here, we visualize ER-mitochondrial contact sites and monitor the localized [Ca(2+)] changes ([Ca(2+)](ER-mt)) using drug-inducible fluorescent interorg...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2010.06.029

    authors: Csordás G,Várnai P,Golenár T,Roy S,Purkins G,Schneider TG,Balla T,Hajnóczky G

    更新日期:2010-07-09 00:00:00

  • A Eukaryotic Sensor for Membrane Lipid Saturation.

    abstract::Maintaining a fluid bilayer is essential for cell signaling and survival. Lipid saturation is a key factor determining lipid packing and membrane fluidity, and it must be tightly controlled to guarantee organelle function and identity. A dedicated eukaryotic mechanism of lipid saturation sensing, however, remains elus...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2016.05.015

    authors: Covino R,Ballweg S,Stordeur C,Michaelis JB,Puth K,Wernig F,Bahrami A,Ernst AM,Hummer G,Ernst R

    更新日期:2016-07-07 00:00:00

  • General and versatile autoinhibition of PLC isozymes.

    abstract::Phospholipase C (PLC) isozymes are directly activated by heterotrimeric G proteins and Ras-like GTPases to hydrolyze phosphatidylinositol 4,5-bisphosphate into the second messengers diacylglycerol and inositol 1,4,5-trisphosphate. Although PLCs play central roles in myriad signaling cascades, the molecular details of ...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2008.06.018

    authors: Hicks SN,Jezyk MR,Gershburg S,Seifert JP,Harden TK,Sondek J

    更新日期:2008-08-08 00:00:00

  • Cyclic GMP-AMP containing mixed phosphodiester linkages is an endogenous high-affinity ligand for STING.

    abstract::The presence of microbial or self DNA in the cytoplasm of mammalian cells is a danger signal detected by the DNA sensor cyclic-GMP-AMP (cGAMP) synthase (cGAS), which catalyzes the production of cGAMP that in turn serves as a second messenger to activate innate immune responses. Here we show that endogenous cGAMP in ma...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2013.05.022

    authors: Zhang X,Shi H,Wu J,Zhang X,Sun L,Chen C,Chen ZJ

    更新日期:2013-07-25 00:00:00

  • The Histone Acetyltransferase Mst2 Protects Active Chromatin from Epigenetic Silencing by Acetylating the Ubiquitin Ligase Brl1.

    abstract::Faithful propagation of functionally distinct chromatin states is crucial for maintaining cellular identity, and its breakdown can lead to diseases such as cancer. Whereas mechanisms that sustain repressed states have been intensely studied, regulatory circuits that protect active chromatin from inactivating signals a...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2017.05.026

    authors: Flury V,Georgescu PR,Iesmantavicius V,Shimada Y,Kuzdere T,Braun S,Bühler M

    更新日期:2017-07-20 00:00:00

  • Transcription of Nearly All Yeast RNA Polymerase II-Transcribed Genes Is Dependent on Transcription Factor TFIID.

    abstract::Previous studies suggested that expression of most yeast mRNAs is dominated by either transcription factor TFIID or SAGA. We re-examined the role of TFIID by rapid depletion of S. cerevisiae TFIID subunits and measurement of changes in nascent transcription. We find that transcription of nearly all mRNAs is strongly d...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2017.08.014

    authors: Warfield L,Ramachandran S,Baptista T,Devys D,Tora L,Hahn S

    更新日期:2017-10-05 00:00:00

  • Phosphorylation of NF-kappa B p65 by PKA stimulates transcriptional activity by promoting a novel bivalent interaction with the coactivator CBP/p300.

    abstract::The transcriptional activity of NF-kappa B is stimulated upon phosphorylation of its p65 subunit on serine 276 by protein kinase A (PKA). The transcriptional coactivator CPB/p300 associates with NF-kappa B p65 through two sites, an N-terminal domain that interacts with the C-terminal region of unphosphorylated p65, an...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/s1097-2765(00)80066-0

    authors: Zhong H,Voll RE,Ghosh S

    更新日期:1998-04-01 00:00:00

  • Just the FACTs: histone H2B ubiquitylation and nucleosome dynamics.

    abstract::In this issue of Molecular Cell, Fleming et al. (2008) show that histone H2B ubiquitylation and FACT function interdependently to facilitate nucleosome reassembly during transcription elongation, thereby demonstrating that histone posttranslational modifications can provide important but transient transcriptional sign...

    journal_title:Molecular cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.molcel.2008.06.012

    authors: Hartzog GA,Quan TK

    更新日期:2008-07-11 00:00:00