The three Rs of transcription: recruit, retain, and recycle.

abstract：:Long DNA palindromes are sites of genome instability (deletions, amplification, and translocations) in both prokaryotic and eukaryotic cells. In Escherichia coli, genetic evidence has suggested that they are sites of DNA cleavage by the SbcCD complex that can be repaired by homologous recombination. Here we obtain in ...

journal_title：Molecular cell

authors： Eykelenboom JK,Blackwood JK,Okely E,Leach DR

更新日期：2008-03-14 00:00:00

abstract：:Organisms must survive a variety of stressful conditions, including sudden temperature increases that damage important cellular structures and interfere with essential functions. In response to heat stress, cells activate an ancient signaling pathway leading to the transient expression of heat shock or heat stress pro...

journal_title：Molecular cell

authors： Richter K,Haslbeck M,Buchner J

更新日期：2010-10-22 00:00:00

abstract：:In a recent issue of Molecular Cell, Shiotani and Zou (2009) elucidate the biochemical mechanism underlying sequential ATM and ATR activation at DNA double-strand breaks, demonstrating that resection transforms ATM substrates into ATR substrates. ...

journal_title：Molecular cell

authors： Rhind N

更新日期：2009-03-27 00:00:00

abstract：:In this issue of Molecular Cell, Hogues et al. (2008) demonstrate a wholesale shift in the key regulatory protein involved in ribosomal protein (RP) synthesis during the evolution of S. cerevisiae and, en passant, raise interesting questions about the relationship between RP genes and telomeres. ...

journal_title：Molecular cell

authors： Bhattacharya A,Warner JR

更新日期：2008-03-14 00:00:00

abstract：:RanBP2/Nup358 is an essential protein with roles in nuclear transport and mitosis, and is one of the few known SUMO E3 ligases. However, why RanBP2 functions in vivo has been unclear: throughout the cell cycle it stably interacts with RanGAP1*SUMO1 and Ubc9, whose binding sites overlap with the E3 ligase region. Here ...

journal_title：Molecular cell

authors： Werner A,Flotho A,Melchior F

更新日期：2012-05-11 00:00:00

abstract：:Brr2 is a unique DExD/H box protein required for catalytic activation and disassembly of the spliceosome. It contains two tandem helicase cassettes that both comprise dual RecA-like domains and a noncanonical Sec63 unit. The latter may bestow the enzyme with unique properties. We have determined crystal structures of ...

journal_title：Molecular cell

authors： Pena V,Jovin SM,Fabrizio P,Orlowski J,Bujnicki JM,Lührmann R,Wahl MC

更新日期：2009-08-28 00:00:00

abstract：:The glycogen synthase kinase-3 (GSK3) family kinases are central cellular regulators highly conserved in all eukaryotes. In Arabidopsis, the GSK3-like kinase BIN2 phosphorylates a range of proteins to control broad developmental processes, and BIN2 is degraded through unknown mechanism upon receptor kinase-mediated br...

journal_title：Molecular cell

authors： Zhu JY,Li Y,Cao DM,Yang H,Oh E,Bi Y,Zhu S,Wang ZY

更新日期：2017-06-01 00:00:00

abstract：:The proper processing, export, localization, translation, and degradation of mRNAs are necessary for regulation of gene expression. These processes are controlled by mRNA-specific regulatory proteins, noncoding RNAs, and core machineries common to most mRNAs. These factors bind the mRNA in large complexes known as mes...

journal_title：Molecular cell

authors： Mitchell SF,Parker R

更新日期：2014-05-22 00:00:00

abstract：:Posttranslational histone modifications play important roles in transcription and other chromatin-based processes. Compared to acetylation, methylation, and phosphorylation, very little is known about the function of histone ubiquitylation. Here, we report the purification and functional characterization of a histone ...

journal_title：Molecular cell

authors： Wang H,Zhai L,Xu J,Joo HY,Jackson S,Erdjument-Bromage H,Tempst P,Xiong Y,Zhang Y

更新日期：2006-05-05 00:00:00

abstract：:Cotranscriptional loading of proteins onto nascent transcripts contributes to the formation of messenger ribonucleoprotein particles (mRNPs) competent for nuclear export. The transcription machinery is believed to play a pivotal role in mRNP assembly, which is at least partially linked to the function of the THO/TREX ...

journal_title：Molecular cell

authors： Jensen TH,Boulay J,Olesen JR,Colin J,Weyler M,Libri D

更新日期：2004-10-22 00:00:00

abstract：:Crossover recombination is essential for accurate chromosome segregation during meiosis. The MutSγ complex, Msh4-Msh5, facilitates crossing over by binding and stabilizing nascent recombination intermediates. We show that these activities are governed by regulated proteolysis. MutSγ is initially inactive for crossing ...

journal_title：Molecular cell

authors： He W,Rao HBDP,Tang S,Bhagwat N,Kulkarni DS,Ma Y,Chang MAW,Hall C,Bragg JW,Manasca HS,Baker C,Verhees GF,Ranjha L,Chen X,Hollingsworth NM,Cejka P,Hunter N

更新日期：2020-04-02 00:00:00

abstract：:In eukaryotes, three-dimensional genome organization is critical for transcriptional regulation of gene expression. Long noncoding RNAs (lncRNAs) can modulate chromatin conformation of spatially related genomic locations within the nucleus. Here, we show that the lncRNA APOLO (AUXIN-REGULATED PROMOTER LOOP) recognizes...

journal_title：Molecular cell

authors： Ariel F,Lucero L,Christ A,Mammarella MF,Jegu T,Veluchamy A,Mariappan K,Latrasse D,Blein T,Liu C,Benhamed M,Crespi M

更新日期：2020-03-05 00:00:00

abstract：:Nup98 is a component of the nuclear pore that plays its primary role in the export of RNAs. Nup98 is expressed in two forms, derived from alternate mRNA splicing. Both forms are processed into two peptides through autoproteolysis mediated by the C-terminal domain of hNup98. The three-dimensional structure of the C-ter...

journal_title：Molecular cell

authors： Hodel AE,Hodel MR,Griffis ER,Hennig KA,Ratner GA,Xu S,Powers MA

更新日期：2002-08-01 00:00:00

abstract：:Previous studies suggested that expression of most yeast mRNAs is dominated by either transcription factor TFIID or SAGA. We re-examined the role of TFIID by rapid depletion of S. cerevisiae TFIID subunits and measurement of changes in nascent transcription. We find that transcription of nearly all mRNAs is strongly d...

journal_title：Molecular cell

authors： Warfield L,Ramachandran S,Baptista T,Devys D,Tora L,Hahn S

更新日期：2017-10-05 00:00:00

abstract：:Ubiquitin (UB)-driven signaling systems permeate biology, and are often integrated with other types of post-translational modifications (PTMs), including phosphorylation. Flux through such pathways is dictated by the fractional stoichiometry of distinct modifications and protein assemblies as well as the spatial organ...

journal_title：Molecular cell

authors： Ordureau A,Münch C,Harper JW

更新日期：2015-05-21 00:00:00

abstract：:Proliferating metazoan cells respond to damage that has the potential to cause genomic instability by restricting the cell division cycle or by initiating apoptosis. The molecular mechanisms determining the balance between these responses are not well understood. Here, we show that the apoptotic initiator protease cas...

journal_title：Molecular cell

authors： Allan LA,Clarke PR

更新日期：2007-04-27 00:00:00

abstract：:Multisubunit protein complexes pose a challenge to the coordinated regulation of individual components. We show how the yeast transactivating factor Met4 functions as a component of the SCF(Met30) ubiquitin ligase to synchronize its own activity with cofactor assembly. Cells maintain Met4 in a dormant state by a regul...

journal_title：Molecular cell

authors： Ouni I,Flick K,Kaiser P

更新日期：2010-12-22 00:00:00

abstract：:Repetitive DNA elements are essential for genome function; in this issue of Molecular Cell, Ishak et al. (2016) describe a novel mechanism of epigenetic repression at these elements that requires pRB-dependent recruitment of EZH2. ...

journal_title：Molecular cell

authors： Sanidas I,Dyson NJ

更新日期：2016-12-15 00:00:00

abstract：:In addition to protein-coding information, mRNAs harbor regulatory sequences necessary for appropriate processing of their precursors. Goren et al. (2006) and Wang et al. (2006) explore the diversity of these signals and the rules by which they function. ...

journal_title：Molecular cell

authors： Guigó R,Valcárcel J

更新日期：2006-07-21 00:00:00

abstract：:There are few tools available for dissecting and elucidating the functions of DNA satellites and other nongenic DNA. To address this, we have explored the experimental potential of DNA sequence-specific drugs containing pyrrole and imidazole amino acids (polyamides). Compounds were synthesized that target different Dr...

journal_title：Molecular cell

authors： Janssen S,Durussel T,Laemmli UK

更新日期：2000-11-01 00:00:00

abstract：:The transcriptional activity of many sequence-specific DNA binding proteins is directly regulated by posttranslational covalent modification. Although this form of regulation was first described nearly two decades ago, it remains poorly understood at a mechanistic level. The prototype for a transcription factor contro...

journal_title：Molecular cell

authors： He C,Hus JC,Sun LJ,Zhou P,Norman DP,Dötsch V,Wei H,Gross JD,Lane WS,Wagner G,Verdine GL

更新日期：2005-10-07 00:00:00

abstract：:Stress granules (SGs) are condensates of mRNPs that form in response to stress. SGs arise by multivalent protein-protein, protein-RNA, and RNA-RNA interactions. However, the role of RNA-RNA interactions in SG assembly remains understudied. Here, we describe a yeast SG reconstitution system that faithfully recapitulate...

journal_title：Molecular cell

authors： Begovich K,Wilhelm JE

更新日期：2020-09-17 00:00:00

abstract：:Nuclear RNA interference (RNAi) pathways work together with histone modifications to regulate gene expression and enact an adaptive response to transposable RNA elements. In the germline, nuclear RNAi can lead to trans-generational epigenetic inheritance (TEI) of gene silencing. We identified and characterized a famil...

journal_title：Molecular cell

authors： Lewis A,Berkyurek AC,Greiner A,Sawh AN,Vashisht A,Merrett S,Flamand MN,Wohlschlegel J,Sarov M,Miska EA,Duchaine TF

更新日期：2020-06-04 00:00:00

abstract：:In bacteria, Hfq is a core RNA chaperone that catalyzes the interaction of mRNAs with regulatory small RNAs (sRNAs). To determine in vivo RNA sequence requirements for Hfq interactions, and to study riboregulation in a bacterial pathogen, Hfq was UV crosslinked to RNAs in enterohemorrhagic Escherichia coli (EHEC). Hfq...

journal_title：Molecular cell

authors： Tree JJ,Granneman S,McAteer SP,Tollervey D,Gally DL

更新日期：2014-07-17 00:00:00

abstract：:N6-methyladenosine (m6A), the most abundant internal mRNA modification, and N6,2'-O-dimethyladenosine (m6Am), found at the first-transcribed nucleotide, are two reversible epitranscriptomic marks. However, the profiles and distribution patterns of m6A and m6Am across human and mouse tissues are poorly characterized. H...

journal_title：Molecular cell

authors： Liu J,Li K,Cai J,Zhang M,Zhang X,Xiong X,Meng H,Xu X,Huang Z,Peng J,Fan J,Yi C

更新日期：2020-01-16 00:00:00

abstract：:Nucleosome loss from a promoter region has recently been described as a potential mechanism for transcriptional regulation. We investigated whether H3/H4 histone chaperones mediate the loss of nucleosomes from the promoter of the yeast PHO5 gene during transcriptional activation. We found that antisilencing function 1...

journal_title：Molecular cell

authors： Adkins MW,Howar SR,Tyler JK

更新日期：2004-06-04 00:00:00

abstract：:Growing resistance of pathogenic bacteria and shortage of antibiotic discovery platforms challenge the use of antibiotics in the clinic. This threat calls for exploration of unconventional sources of antibiotics and identification of inhibitors able to eradicate resistant bacteria. Here we describe a different class o...

journal_title：Molecular cell

authors： Pantel L,Florin T,Dobosz-Bartoszek M,Racine E,Sarciaux M,Serri M,Houard J,Campagne JM,de Figueiredo RM,Midrier C,Gaudriault S,Givaudan A,Lanois A,Forst S,Aumelas A,Cotteaux-Lautard C,Bolla JM,Vingsbo Lundberg C,Huseby

更新日期：2018-04-05 00:00:00

abstract：:p300/CBP and PCAF coactivators have acetyltransferase activities and regulate transcription, cell cycle progression, and differentiation. They are both required for MyoD activity and muscle differentiation. Nevertheless, their roles must be different since the acetyltransferase activity of PCAF but not of p300 is invo...

journal_title：Molecular cell

authors： Sartorelli V,Puri PL,Hamamori Y,Ogryzko V,Chung G,Nakatani Y,Wang JY,Kedes L

更新日期：1999-11-01 00:00:00

abstract：:Nuclear import of proteins containing a classical nuclear localization signal (NLS) involves NLS recognition by importin alpha, which associates with importin beta via the IBB domain. Other proteins, including parathyroid hormone-related protein (PTHrP), are imported into the nucleus by direct interaction with importi...

journal_title：Molecular cell

authors： Cingolani G,Bednenko J,Gillespie MT,Gerace L

更新日期：2002-12-01 00:00:00

abstract：:When inappropriate DNA structures arise, they are sensed by DNA structure-dependent checkpoint pathways and subsequently repaired. Recruitment of checkpoint proteins to such structures precedes recruitment of proteins involved in DNA metabolism. Thus, checkpoints can regulate DNA metabolism. We show that fission yeast...

journal_title：Molecular cell

authors： Furuya K,Miyabe I,Tsutsui Y,Paderi F,Kakusho N,Masai H,Niki H,Carr AM

更新日期：2010-11-24 00:00:00