Changing of the guard: how ATM hands off DNA double-strand break signaling to ATR.

abstract：:It is generally believed that eukaryotic ribosomes first associate with mRNA in the cytoplasm. However, we show with chromosomal immunostaining and in situ hybridization that ribosomal subunits are present at transcription sites of Drosophila salivary gland chromosomes. Immunostaining was carried out with antibodies s...

journal_title：Molecular cell

authors： Brogna S,Sato TA,Rosbash M

更新日期：2002-07-01 00:00:00

abstract：:To map the genomic interaction sites of chromatin proteins, two related methods were developed and experimentally explored in Saccharomyces cerevisiae. The ChIC method (chromatin immunocleavage) consists of tethering a fusion protein (pA-MN) consisting of micrococcal nuclease (MN) and staphylococcal protein A to speci...

journal_title：Molecular cell

authors： Schmid M,Durussel T,Laemmli UK

更新日期：2004-10-08 00:00:00

abstract：:Protein kinase B/Akt plays crucial roles in promoting cell survival and mediating insulin responses. The enzyme is stimulated by phosphorylation at two regulatory sites: Thr 309 of the activation segment and Ser 474 of the hydrophobic motif, a conserved feature of many AGC kinases. Analysis of the crystal structures o...

journal_title：Molecular cell

authors： Yang J,Cron P,Thompson V,Good VM,Hess D,Hemmings BA,Barford D

更新日期：2002-06-01 00:00:00

abstract：:The papillomavirus major late protein, L1, forms the pentameric assembly unit of the viral shell. Recombinant HPV16 L1 pentamers assemble in vitro into capsid-like structures, and truncation of ten N-terminal residues leads to a homogeneous preparation of 12-pentamer, icosahedral particles. X-ray crystallographic anal...

journal_title：Molecular cell

authors： Chen XS,Garcea RL,Goldberg I,Casini G,Harrison SC

更新日期：2000-03-01 00:00:00

abstract：:Both EZH2 and NF-κB contribute to aggressive breast cancer, yet whether the two oncogenic factors have functional crosstalk in breast cancer is unknown. Here, we uncover an unexpected role of EZH2 in conferring the constitutive activation of NF-κB target gene expression in ER-negative basal-like breast cancer cells. T...

journal_title：Molecular cell

authors： Lee ST,Li Z,Wu Z,Aau M,Guan P,Karuturi RK,Liou YC,Yu Q

更新日期：2011-09-02 00:00:00

abstract：:In this issue of Molecular Cell,Sharma et al. (2019) show that normal cell growth requires conversion of an arginine residue in the RNA polymerase II C-terminal domain (CTD) to citrulline, uncovering a potential regulatory pathway involving opposing arginine modifications. ...

journal_title：Molecular cell

authors： Corden JL

更新日期：2019-01-03 00:00:00

abstract：:Prokaryotes use a mechanism called priming to update their CRISPR immunological memory to rapidly counter revisiting, mutated viruses, and plasmids. Here we have determined how new spacers are produced and selected for integration into the CRISPR array during priming. We show that Cas3 couples CRISPR interference to a...

journal_title：Molecular cell

authors： Künne T,Kieper SN,Bannenberg JW,Vogel AI,Miellet WR,Klein M,Depken M,Suarez-Diez M,Brouns SJ

更新日期：2016-09-01 00:00:00

abstract：:Autophagic turnover of intracellular constituents is critical for cellular housekeeping, nutrient recycling, and various aspects of growth and development in eukaryotes. Here we show that autophagy impacts the other major degradative route involving the ubiquitin-proteasome system by eliminating 26S proteasomes, a pro...

journal_title：Molecular cell

authors： Marshall RS,Li F,Gemperline DC,Book AJ,Vierstra RD

更新日期：2015-06-18 00:00:00

abstract：:Stress granules (SGs) are cytoplasmic assemblies of mRNPs stalled in translation initiation. They are induced by various stress conditions, including exposure to the environmental toxin and carcinogen arsenic. While perturbed SG turnover is linked to the pathogenesis of neurodegenerative diseases, the molecular mechan...

journal_title：Molecular cell

authors： Turakhiya A,Meyer SR,Marincola G,Böhm S,Vanselow JT,Schlosser A,Hofmann K,Buchberger A

更新日期：2018-06-07 00:00:00

abstract：:Multi-subunit SMC complexes control chromosome superstructure and promote chromosome disjunction, conceivably by actively translocating along DNA double helices. SMC subunits comprise an ABC ATPase "head" and a "hinge" dimerization domain connected by a 49 nm coiled-coil "arm." The heads undergo ATP-dependent engageme...

journal_title：Molecular cell

authors： Diebold-Durand ML,Lee H,Ruiz Avila LB,Noh H,Shin HC,Im H,Bock FP,Bürmann F,Durand A,Basfeld A,Ham S,Basquin J,Oh BH,Gruber S

更新日期：2017-07-20 00:00:00

abstract：:The separation of sister chromatids in anaphase depends on the dissociation of cohesin from chromosomes. In vertebrates, some cohesin is removed from chromosomes at the onset of anaphase by proteolytic cleavage. In contrast, the bulk of cohesin is removed from chromosomes already in prophase and prometaphase by an unk...

journal_title：Molecular cell

authors： Sumara I,Vorlaufer E,Stukenberg PT,Kelm O,Redemann N,Nigg EA,Peters JM

更新日期：2002-03-01 00:00:00

abstract：:RNA:DNA hybrids in the genome are constantly being generated as a by-product of transcription; in this issue, two papers, from Helmrich et al. (2011) and Wahba et al. (2011), provide insight into how RNA:DNA hybrids lead to genetic instability. ...

journal_title：Molecular cell

authors： Potenski CJ,Klein HL

更新日期：2011-12-23 00:00:00

abstract：:The transcriptional activity of NF-kappa B is stimulated upon phosphorylation of its p65 subunit on serine 276 by protein kinase A (PKA). The transcriptional coactivator CPB/p300 associates with NF-kappa B p65 through two sites, an N-terminal domain that interacts with the C-terminal region of unphosphorylated p65, an...

journal_title：Molecular cell

authors： Zhong H,Voll RE,Ghosh S

更新日期：1998-04-01 00:00:00

abstract：:X-linked mental retardation (XLMR) is an inherited disorder that mostly affects males and is caused by mutations in genes located on the X chromosome. Here, we show that the XLMR protein PHF8 and a C. elegans homolog F29B9.2 catalyze demethylation of di- and monomethylated lysine 9 of histone H3 (H3K9me2/me1). The PHD...

journal_title：Molecular cell

authors： Kleine-Kohlbrecher D,Christensen J,Vandamme J,Abarrategui I,Bak M,Tommerup N,Shi X,Gozani O,Rappsilber J,Salcini AE,Helin K

更新日期：2010-04-23 00:00:00

abstract：:CREB-binding protein (CBP) possesses an intrinsic acetyltransferase activity capable of acetylating nucleosomal histones as well as several nonhistone proteins. Here, it is shown that CBP can acetylate hepatocyte nuclear factor-4 (HNF-4), a member of the nuclear hormone receptor family, at lysine residues within the n...

journal_title：Molecular cell

authors： Soutoglou E,Katrakili N,Talianidis I

更新日期：2000-04-01 00:00:00

abstract：:The small nuclear RNA (snRNA) genes have been widely used as a model system for understanding transcriptional regulation due to the unique aspects of their promoter structure, selectivity for either RNA polymerase (Pol) II or III, and because of their unique mechanism of termination that is tightly linked with the pro...

journal_title：Molecular cell

authors： Hu D,Smith ER,Garruss AS,Mohaghegh N,Varberg JM,Lin C,Jackson J,Gao X,Saraf A,Florens L,Washburn MP,Eissenberg JC,Shilatifard A

更新日期：2013-08-22 00:00:00

abstract：:Ubiquitin-mediated proteolysis regulates the activity of diverse receptor systems. Here, we identify Smurf2, a C2-WW-HECT domain ubiquitin ligase and show that Smurf2 associates constitutively with Smad7. Smurf2 is nuclear, but binding to Smad7 induces export and recruitment to the activated TGF beta receptor, where i...

journal_title：Molecular cell

authors： Kavsak P,Rasmussen RK,Causing CG,Bonni S,Zhu H,Thomsen GH,Wrana JL

更新日期：2000-12-01 00:00:00

abstract：:Physical and genetic studies verify that the DNA binding domain of protein gpNu1 (which initiates packaging of phage lambda DNA) is a winged helix-turn-helix (w HTH) and that gpNu1 dimers bind sites that are brought close through DNA bending. ...

journal_title：Molecular cell

authors： Campbell A

更新日期：2002-05-01 00:00:00

abstract：:The S. cerevisiae SCFCdc4p ubiquitin-protein ligase complex promotes cell cycle transitions through degradation of cell cycle regulators. To investigate SCFCdc4p regulation in vivo, we examined the stability of individual SCFCdc4p components. Whereas Cdc53p and Skp1p were stable, Cdc4p, the F box-containing component ...

journal_title：Molecular cell

authors： Zhou P,Howley PM

更新日期：1998-11-01 00:00:00

abstract：:Despite being sessile organisms constantly exposed to potential pathogens and pests, plants are surprisingly resilient to infections. Plants can detect invaders via the recognition of pathogen-associated molecular patterns (PAMPs) by pattern recognition receptors (PRRs). Plant PRRs are surface-localized receptor-like ...

journal_title：Molecular cell

authors： Macho AP,Zipfel C

更新日期：2014-04-24 00:00:00

abstract：:Ten E. coli aminoacyl-tRNAs (aa-tRNAs) were assessed for their ability to decode cognate codons on E. coli ribosomes by using three assays that evaluate the key steps in the decoding pathway. Despite a wide variety of structural features, each aa-tRNA exhibited similar kinetic and thermodynamic properties in each assa...

journal_title：Molecular cell

authors： Ledoux S,Uhlenbeck OC

更新日期：2008-07-11 00:00:00

abstract：:The early detection by the host of invading microorganisms, including viruses, depends on a limited number of specific receptors that recognize pathogen-associated molecular patterns (PAMPs). A few of these PAMPs, including ssRNA and dsRNA, are recognized by Toll-like receptors (TLR)-7/8 and TLR3, respectively. Activa...

journal_title：Molecular cell

authors： Meylan E,Tschopp J

更新日期：2006-06-09 00:00:00

abstract：:The fidelity of chromosome segregation depends on the spindle assembly checkpoint (SAC). In the presence of unattached kinetochores, anaphase is delayed when three SAC components (Mad2, Mad3/BubR1, and Bub3) inhibit Cdc20, the activating subunit of the anaphase-promoting complex (APC/C). We analyzed the role of Cdc20 ...

journal_title：Molecular cell

authors： Foster SA,Morgan DO

更新日期：2012-09-28 00:00:00

abstract：:Proteins with annealing activity are newly identified ATP-dependent motors that can rewind RPA-coated complementary single-stranded DNA bubbles. AH2 (annealing helicase 2, also named as ZRANB3) is the second protein with annealing activity, the function of which is still unknown. Here, we report that AH2 is recruited ...

journal_title：Molecular cell

authors： Yuan J,Ghosal G,Chen J

更新日期：2012-08-10 00:00:00

abstract：:B and T cell receptor gene assembly by V(D)J recombination is tightly regulated during lymphoid development. The mechanisms involved in this regulation are poorly understood. Here we show that nucleosomal DNA is refractory to V(D)J cleavage. However, the presence of HMG1, a chromatin-associated nonhistone DNA-binding ...

journal_title：Molecular cell

authors： Kwon J,Imbalzano AN,Matthews A,Oettinger MA

更新日期：1998-12-01 00:00:00

abstract：:Oncogene-induced senescence (OIS) is a tumor-suppressive mechanism typified by stable proliferative arrest, a persistent DNA damage response, and the senescence-associated secretory phenotype (SASP), which helps to maintain the senescent state and triggers bystander senescence in a paracrine fashion. Here, we demonstr...

journal_title：Molecular cell

authors： Chen H,Ruiz PD,McKimpson WM,Novikov L,Kitsis RN,Gamble MJ

更新日期：2015-09-03 00:00:00

abstract：:Posttranslational histone modifications participate in modulating the structure and function of chromatin. Promoters of transcribed genes are enriched with K4 trimethylation and hyperacetylation on the N-terminal tail of histone H3. Recently, PHD finger proteins, like Yng1 in the NuA3 HAT complex, were shown to intera...

journal_title：Molecular cell

authors： Taverna SD,Ilin S,Rogers RS,Tanny JC,Lavender H,Li H,Baker L,Boyle J,Blair LP,Chait BT,Patel DJ,Aitchison JD,Tackett AJ,Allis CD

更新日期：2006-12-08 00:00:00

abstract：:Spt6 is an essential histone chaperone that mediates nucleosome reassembly during gene transcription. Spt6 also associates with RNA polymerase II (RNAPII) via a tandem Src2 homology domain. However, the significance of Spt6-RNAPII interaction is not well understood. Here, we show that Spt6 recruitment to genes and the...

journal_title：Molecular cell

authors： Dronamraju R,Hepperla AJ,Shibata Y,Adams AT,Magnuson T,Davis IJ,Strahl BD

更新日期：2018-06-21 00:00:00

abstract：:RNA-mediated gene silencing in human cells requires the accurate generation of ∼22 nt microRNAs (miRNAs) from double-stranded RNA substrates by the endonuclease Dicer. Although the phylogenetically conserved RNA-binding proteins TRBP and PACT are known to contribute to this process, their mode of Dicer binding and the...

journal_title：Molecular cell

authors： Wilson RC,Tambe A,Kidwell MA,Noland CL,Schneider CP,Doudna JA

更新日期：2015-02-05 00:00:00

abstract：:Study of mutant phenotypes is a fundamental method for understanding gene function. The construction of a near-complete collection of yeast knockouts (YKO) and the unique molecular barcodes (or TAGs) that identify each strain has enabled quantitative functional profiling of Saccharomyces cerevisiae. By using these TAG...

journal_title：Molecular cell

authors： Pan X,Yuan DS,Xiang D,Wang X,Sookhai-Mahadeo S,Bader JS,Hieter P,Spencer F,Boeke JD

更新日期：2004-11-05 00:00:00