Abstract:
:Multisubunit protein complexes pose a challenge to the coordinated regulation of individual components. We show how the yeast transactivating factor Met4 functions as a component of the SCF(Met30) ubiquitin ligase to synchronize its own activity with cofactor assembly. Cells maintain Met4 in a dormant state by a regulatory ubiquitin chain assembled by SCF(Met30). Nutritional and heavy-metal stress block Met4 ubiquitylation resulting in Met4 activation, which induces a stress-response program including cell-cycle arrest. Met4 relies on assembly with various cofactors for promoter binding. We report here that the stability of these DNA-binding cofactors is regulated by SCF(Met30). Remarkably, the transcriptional activator Met4 functions as a substrate-specificity factor in the context of SCF(Met30/Met4) to coordinate cofactor degradation with its own activity status. Our results establish an additional layer for substrate recruitment by SCF ubiquitin ligases and provide conceptual insight into coordinated regulation of protein complexes.
journal_name
Mol Celljournal_title
Molecular cellauthors
Ouni I,Flick K,Kaiser Pdoi
10.1016/j.molcel.2010.11.018subject
Has Abstractpub_date
2010-12-22 00:00:00pages
954-64issue
6eissn
1097-2765issn
1097-4164pii
S1097-2765(10)00883-Xjournal_volume
40pub_type
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