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A transcriptional activator is part of an SCF ubiquitin ligase to control degradation of its cofactors.

Abstract：

:Multisubunit protein complexes pose a challenge to the coordinated regulation of individual components. We show how the yeast transactivating factor Met4 functions as a component of the SCF(Met30) ubiquitin ligase to synchronize its own activity with cofactor assembly. Cells maintain Met4 in a dormant state by a regulatory ubiquitin chain assembled by SCF(Met30). Nutritional and heavy-metal stress block Met4 ubiquitylation resulting in Met4 activation, which induces a stress-response program including cell-cycle arrest. Met4 relies on assembly with various cofactors for promoter binding. We report here that the stability of these DNA-binding cofactors is regulated by SCF(Met30). Remarkably, the transcriptional activator Met4 functions as a substrate-specificity factor in the context of SCF(Met30/Met4) to coordinate cofactor degradation with its own activity status. Our results establish an additional layer for substrate recruitment by SCF ubiquitin ligases and provide conceptual insight into coordinated regulation of protein complexes.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Ouni I,Flick K,Kaiser P

doi

10.1016/j.molcel.2010.11.018

subject

Has Abstract

pub_date

2010-12-22 00:00:00

pages

954-64

issue

6

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(10)00883-X

journal_volume

40

pub_type

杂志文章

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