miR-14 regulates autophagy during developmental cell death by targeting ip3-kinase 2.

Abstract:

:Macroautophagy (autophagy) is a lysosome-dependent degradation process that has been implicated in age-associated diseases. Autophagy is involved in both cell survival and cell death, but little is known about the mechanisms that distinguish its use during these distinct cell fates. Here, we identify the microRNA miR-14 as being both necessary and sufficient for autophagy during developmentally regulated cell death in Drosophila. Loss of miR-14 prevented induction of autophagy during salivary gland cell death, but had no effect on starvation-induced autophagy in the fat body. Moreover, misexpression of miR-14 was sufficient to prematurely induce autophagy in salivary glands, but not in the fat body. Importantly, miR-14 regulates this context-specific autophagy through its target, inositol 1,4,5-trisphosphate kinase 2 (ip3k2), thereby affecting inositol 1,4,5-trisphosphate (IP3) signaling and calcium levels during salivary gland cell death. This study provides in vivo evidence of microRNA regulation of autophagy through modulation of IP3 signaling.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Nelson C,Ambros V,Baehrecke EH

doi

10.1016/j.molcel.2014.09.011

subject

Has Abstract

pub_date

2014-11-06 00:00:00

pages

376-88

issue

3

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(14)00718-7

journal_volume

56

pub_type

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