Abstract:
:Stress granules are mRNA-protein assemblies formed from nontranslating mRNAs. Stress granules are important in the stress response and may contribute to some degenerative diseases. Here, we describe the stress granule transcriptome of yeast and mammalian cells through RNA-sequencing (RNA-seq) analysis of purified stress granule cores and single-molecule fluorescence in situ hybridization (smFISH) validation. While essentially every mRNA, and some noncoding RNAs (ncRNAs), can be targeted to stress granules, the targeting efficiency varies from <1% to >95%. mRNA accumulation in stress granules correlates with longer coding and UTR regions and poor translatability. Quantifying the RNA-seq analysis by smFISH reveals that only 10% of bulk mRNA molecules accumulate in mammalian stress granules and that only 185 genes have more than 50% of their mRNA molecules in stress granules. These results suggest that stress granules may not represent a specific biological program of messenger ribonucleoprotein (mRNP) assembly, but instead form by condensation of nontranslating mRNPs in proportion to their length and lack of association with ribosomes.
journal_name
Mol Celljournal_title
Molecular cellauthors
Khong A,Matheny T,Jain S,Mitchell SF,Wheeler JR,Parker Rdoi
10.1016/j.molcel.2017.10.015subject
Has Abstractpub_date
2017-11-16 00:00:00pages
808-820.e5issue
4eissn
1097-2765issn
1097-4164pii
S1097-2765(17)30790-6journal_volume
68pub_type
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