Abstract:
:The heterodimeric nuclear cap binding complex (CBC) binds to 5'-capped polymerase II transcripts. It enhances the efficiency of several mRNA maturation steps and is essential for U snRNA nuclear export in multicellular eukaryotes. The 2A crystal structure of human CBC shows that the large subunit, CBP80, comprises three domains, each containing consecutive helical hairpins and resembling the so-called MIF4G domain found in several other proteins involved in RNA metabolism. The small subunit, CPB20, has an RNP fold and associates with the second and third domains of CBP80. Site-directed mutagenesis revealed 4 residues of CBP20 which are critical for cap binding. A model for cap binding is proposed based on these results and the known mode of binding of RNA to RNP domains.
journal_name
Mol Celljournal_title
Molecular cellauthors
Mazza C,Ohno M,Segref A,Mattaj IW,Cusack Sdoi
10.1016/s1097-2765(01)00299-4subject
Has Abstractpub_date
2001-08-01 00:00:00pages
383-96issue
2eissn
1097-2765issn
1097-4164pii
S1097-2765(01)00299-4journal_volume
8pub_type
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