EM visualization of transcription by RNA polymerase II: downstream termination requires a poly(A) signal but not transcript cleavage.

Abstract:

:We have used EM visualization of active genes on plasmid vectors in Xenopus oocyte nuclei to investigate the relationship between poly(A) signals and RNA polymerase II transcription termination. Although a functional poly(A) signal is required for efficient termination, cotranscriptional RNA cleavage at the poly(A) site is not. Furthermore, the phenomena of termination and cotranscriptional RNA cleavage can be uncoupled, and the efficiency of both varies independently on different copies of the same plasmid template in the same oocyte nucleus. The combined observations are consistent with a scenario in which there is template-specific addition to Pol II (presumably at the promoter) of elongation and/or RNA processing factors, which are altered upon passage through a poly(A) signal, resulting in termination and, in some cases, cotranscriptional RNA cleavage.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Osheim YN,Proudfoot NJ,Beyer AL

doi

10.1016/s1097-2765(00)80465-7

subject

Has Abstract

pub_date

1999-03-01 00:00:00

pages

379-87

issue

3

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(00)80465-7

journal_volume

3

pub_type

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