Abstract:
:We have used EM visualization of active genes on plasmid vectors in Xenopus oocyte nuclei to investigate the relationship between poly(A) signals and RNA polymerase II transcription termination. Although a functional poly(A) signal is required for efficient termination, cotranscriptional RNA cleavage at the poly(A) site is not. Furthermore, the phenomena of termination and cotranscriptional RNA cleavage can be uncoupled, and the efficiency of both varies independently on different copies of the same plasmid template in the same oocyte nucleus. The combined observations are consistent with a scenario in which there is template-specific addition to Pol II (presumably at the promoter) of elongation and/or RNA processing factors, which are altered upon passage through a poly(A) signal, resulting in termination and, in some cases, cotranscriptional RNA cleavage.
journal_name
Mol Celljournal_title
Molecular cellauthors
Osheim YN,Proudfoot NJ,Beyer ALdoi
10.1016/s1097-2765(00)80465-7subject
Has Abstractpub_date
1999-03-01 00:00:00pages
379-87issue
3eissn
1097-2765issn
1097-4164pii
S1097-2765(00)80465-7journal_volume
3pub_type
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