A dynamic mechanism for AKAP binding to RII isoforms of cAMP-dependent protein kinase.

abstract：:Siz1 is a founding member of the Siz/PIAS RING family of SUMO E3 ligases. The X-ray structure of an active Siz1 ligase revealed an elongated tripartite architecture comprised of an N-terminal PINIT domain, a central zinc-containing RING-like SP-RING domain, and a C-terminal domain we term the SP-CTD. Structure-based m...

journal_title：Molecular cell

authors： Yunus AA,Lima CD

更新日期：2009-09-11 00:00:00

abstract：:The bHLH factors HAND1 and HAND2 are required for heart, vascular, neuronal, limb, and extraembryonic development. Unlike most bHLH proteins, HAND factors exhibit promiscuous dimerization properties. We report that phosphorylation/dephosphorylation via PKA, PKC, and a specific heterotrimeric protein phosphatase 2A (PP...

journal_title：Molecular cell

authors： Firulli BA,Howard MJ,McDaid JR,McIlreavey L,Dionne KM,Centonze VE,Cserjesi P,Virshup DM,Firulli AB

更新日期：2003-11-01 00:00:00

abstract：:A remarkable paper from the de Lange lab (Wang et al., 2004) in a recent issue of Cell reveals that homologous recombination can result in the abrupt shortening of telomeres in a process that appears to involve reciprocal crossing over within the t-loop structure that protects chromosome ends. ...

journal_title：Molecular cell

authors： Haber JE

更新日期：2004-11-19 00:00:00

abstract：:YAP/TEAD are nuclear effectors of the Hippo pathway, regulating organ size and tumorigenesis largely through promoter-associated function. However, their function as enhancer regulators remains poorly understood. Through an in vivo proximity-dependent labeling (BioID) technique, we identified YAP1 and TEAD4 protein as...

journal_title：Molecular cell

authors： Zhu C,Li L,Zhang Z,Bi M,Wang H,Su W,Hernandez K,Liu P,Chen J,Chen M,Huang TH,Chen L,Liu Z

更新日期：2019-08-22 00:00:00

abstract：:Environmental cues provoke rapid transitions in gene expression to support growth and cellular plasticity through incompletely understood mechanisms. Lin28 RNA-binding proteins have evolutionarily conserved roles in post-transcriptional coordination of pro-growth gene expression, but signaling pathways allowing trophi...

journal_title：Molecular cell

authors： Amen AM,Ruiz-Garzon CR,Shi J,Subramanian M,Pham DL,Meffert MK

更新日期：2017-02-02 00:00:00

abstract：:mRNAs form ribonucleoprotein complexes (mRNPs) by association with proteins that are crucial for mRNA metabolism. While the mRNP proteome has been well characterized, little is known about mRNP organization. Using a single-molecule approach, we show that mRNA conformation changes depending on its cellular localization...

journal_title：Molecular cell

authors： Adivarahan S,Livingston N,Nicholson B,Rahman S,Wu B,Rissland OS,Zenklusen D

更新日期：2018-11-15 00:00:00

abstract：:The molecular structures of crossover (CO) and noncrossover (NCO) intermediates were determined by sequencing the products formed when a gapped plasmid was repaired using a diverged chromosomal template. Analyses were done in the absence of mismatch repair (MMR) to allow efficient detection of strand-transfer intermed...

journal_title：Molecular cell

authors： Mitchel K,Zhang H,Welz-Voegele C,Jinks-Robertson S

更新日期：2010-04-23 00:00:00

abstract：:PTPA, an essential and specific activator of protein phosphatase 2A (PP2A), functions as a peptidyl prolyl isomerase (PPIase). We present here the crystal structures of human PTPA and of the two yeast orthologs (Ypa1 and Ypa2), revealing an all alpha-helical protein fold that is radically different from other PPIases....

journal_title：Molecular cell

authors： Leulliot N,Vicentini G,Jordens J,Quevillon-Cheruel S,Schiltz M,Barford D,van Tilbeurgh H,Goris J

更新日期：2006-08-04 00:00:00

abstract：:The CRISPR-associated protein Cas12a (Cpf1), which has been repurposed for genome editing, possesses two distinct nuclease activities: endoribonuclease activity for processing its own guide RNAs and RNA-guided DNase activity for target DNA cleavage. To elucidate the molecular basis of both activities, we determined cr...

journal_title：Molecular cell

authors： Swarts DC,van der Oost J,Jinek M

更新日期：2017-04-20 00:00:00

abstract：:EGFR activates phosphatidylinositide 3-kinase (PI3K), but the mechanism underlying this activation is not completely understood. We demonstrated here that EGFR activation resulted in lysine acetyltransferase 5 (KAT5)-mediated K395 acetylation of the platelet isoform of phosphofructokinase 1 (PFKP) and subsequent trans...

journal_title：Molecular cell

authors： Lee JH,Liu R,Li J,Wang Y,Tan L,Li XJ,Qian X,Zhang C,Xia Y,Xu D,Guo W,Ding Z,Du L,Zheng Y,Chen Q,Lorenzi PL,Mills GB,Jiang T,Lu Z

更新日期：2018-04-19 00:00:00

abstract：:SPT5 and its binding partner SPT4 function in both positively and negatively regulating transcriptional elongation. The demonstration that SPT5 and RNA polymerase II are targets for phosphorylation by CDK9/cyclin T1 indicates that posttranslational modifications of these factors are important in regulating the elongat...

journal_title：Molecular cell

authors： Kwak YT,Guo J,Prajapati S,Park KJ,Surabhi RM,Miller B,Gehrig P,Gaynor RB

更新日期：2003-04-01 00:00:00

abstract：:The intracellular regulation of cell death pathways by cIAPs has been enigmatic. Here we show that loss of cIAPs promotes the spontaneous formation of an intracellular platform that activates either apoptosis or necroptosis. This 2 MDa intracellular complex that we designate "Ripoptosome" is necessary but not sufficie...

journal_title：Molecular cell

authors： Feoktistova M,Geserick P,Kellert B,Dimitrova DP,Langlais C,Hupe M,Cain K,MacFarlane M,Häcker G,Leverkus M

更新日期：2011-08-05 00:00:00

abstract：:Deep orange and carnation are two of the classic eye color genes in Drosophila. Here, we demonstrate that Deep orange is part of a protein complex that localizes to endosomal compartments. A second component of this complex is Carnation, a homolog of Sec1p-like regulators of membrane fusion. Because complete loss of d...

journal_title：Molecular cell

authors： Sevrioukov EA,He JP,Moghrabi N,Sunio A,Krämer H

更新日期：1999-10-01 00:00:00

abstract：:Transcriptional coregulators, rather than ligand signals, are suspected to confer context and pathway specificity to nuclear receptor signaling, but the identity of such specifying coregulators and the underlying molecular mechanisms remain largely enigmatic. Here we address this issue in metabolic oxysterol receptor ...

journal_title：Molecular cell

authors： Jakobsson T,Venteclef N,Toresson G,Damdimopoulos AE,Ehrlund A,Lou X,Sanyal S,Steffensen KR,Gustafsson JA,Treuter E

更新日期：2009-05-14 00:00:00

abstract：:The duplication of eukaryotic genomes involves the replication of DNA from multiple origins of replication. In S phase, two sister replisomes assemble at each active origin, and they replicate DNA in opposite directions. Little is known about the functional relationship between sister replisomes. Some data imply that ...

journal_title：Molecular cell

authors： Yardimci H,Loveland AB,Habuchi S,van Oijen AM,Walter JC

更新日期：2010-12-10 00:00:00

abstract：:The Raf/MEK/ERK and PI3K/Akt pathways are prominent effectors of oncogenic Ras. These pathways negatively regulate each other, but the mechanism involved is incompletely understood. We now identify MEK1 as an essential regulator of lipid/protein phosphatase PTEN, through which it controls phosphatidylinositol-3-phosph...

journal_title：Molecular cell

authors： Zmajkovicova K,Jesenberger V,Catalanotti F,Baumgartner C,Reyes G,Baccarini M

更新日期：2013-04-11 00:00:00

abstract：:In this issue of Molecular Cell, Weinert et al. (2013) demonstrate that the intermediary metabolite acetyl-phosphate is an important acetyl donor that contributes to global protein acetylation in growth-arrested E. coli. ...

journal_title：Molecular cell

authors： Verdin E,Ott M

更新日期：2013-07-25 00:00:00

abstract：:Both H4K16 acetylation and H3K4 trimethylation are required for gene activation. However, it is still largely unclear how these modifications are orchestrated by transcriptional factors. Here, we analyzed the mechanism of the transcriptional activation by FOXP3, an X-linked suppressor of autoimmune diseases and cancer...

journal_title：Molecular cell

authors： Katoh H,Qin ZS,Liu R,Wang L,Li W,Li X,Wu L,Du Z,Lyons R,Liu CG,Liu X,Dou Y,Zheng P,Liu Y

更新日期：2011-12-09 00:00:00

abstract：:Nucleolytic resection of DNA double-strand breaks is the crucial first step in their repair via homologous recombination. New findings by Tkáč et al. (2016) published in this issue of Molecular Cell identify HELB as a novel, cell-cycle-specific negative regulator of DNA end resection. ...

journal_title：Molecular cell

authors： Daley JM,Sung P

更新日期：2016-02-04 00:00:00

abstract：:The ability to measure human aging from molecular profiles has practical implications in many fields, including disease prevention and treatment, forensics, and extension of life. Although chronological age has been linked to changes in DNA methylation, the methylome has not yet been used to measure and compare human ...

journal_title：Molecular cell

authors： Hannum G,Guinney J,Zhao L,Zhang L,Hughes G,Sadda S,Klotzle B,Bibikova M,Fan JB,Gao Y,Deconde R,Chen M,Rajapakse I,Friend S,Ideker T,Zhang K

更新日期：2013-01-24 00:00:00

abstract：:Previous studies suggested that expression of most yeast mRNAs is dominated by either transcription factor TFIID or SAGA. We re-examined the role of TFIID by rapid depletion of S. cerevisiae TFIID subunits and measurement of changes in nascent transcription. We find that transcription of nearly all mRNAs is strongly d...

journal_title：Molecular cell

authors： Warfield L,Ramachandran S,Baptista T,Devys D,Tora L,Hahn S

更新日期：2017-10-05 00:00:00

abstract：:Alternative splicing (AS) is a widespread process underlying the generation of transcriptomic and proteomic diversity and is frequently misregulated in human disease. Accordingly, an important goal of biomedical research is the development of tools capable of comprehensively, accurately, and efficiently profiling AS. ...

journal_title：Molecular cell

authors： Sterne-Weiler T,Weatheritt RJ,Best AJ,Ha KCH,Blencowe BJ

更新日期：2018-10-04 00:00:00

abstract：:The phosphoinositide (PI)-3-kinase-related kinase (PIKK) family proteins Tel1p and Mec1p have been implicated in the telomere integrity of Saccharomyces cerevisiae. However, the mechanism of PIKK-mediated telomere length control remains unclear. Here, we show that Tel1p and Mec1p are recruited to the telomeres at spec...

journal_title：Molecular cell

authors： Takata H,Kanoh Y,Gunge N,Shirahige K,Matsuura A

更新日期：2004-05-21 00:00:00

abstract：:JunD is the most broadly expressed member of the Jun family and the AP-1 transcription factor complex. Primary fibroblasts lacking JunD displayed p53-dependent growth arrest, upregulated p19(Arf) expression, and premature senescence. In contrast, immortalized cell lines lacking JunD showed increased proliferation and ...

journal_title：Molecular cell

authors： Weitzman JB,Fiette L,Matsuo K,Yaniv M

更新日期：2000-11-01 00:00:00

abstract：:In Xer site-specific recombination, sequential DNA strand exchange reactions are catalyzed by a heterotetrameric complex composed of two recombinases, XerC and XerD. It is demonstrated that XerC and XerD catalytic activity is controlled by an interaction involving the C-terminal end of each protein (the donor region) ...

journal_title：Molecular cell

authors： Hallet B,Arciszewska LK,Sherratt DJ

更新日期：1999-12-01 00:00:00

abstract：:MicroRNAs (miRNAs) are essential components of gene regulation, but identification of miRNA targets remains a major challenge. Most target prediction and discovery relies on perfect complementarity of the miRNA seed to the 3' untranslated region (UTR). However, it is unclear to what extent miRNAs target sites without ...

journal_title：Molecular cell

authors： Loeb GB,Khan AA,Canner D,Hiatt JB,Shendure J,Darnell RB,Leslie CS,Rudensky AY

更新日期：2012-12-14 00:00:00

abstract：:Cell pattern in the ventral neural tube is organized by Sonic hedgehog (Shh) secreted by floor plate cells. To assay the range of direct Shh action, we developed a general method for blocking transduction of Hedgehog (Hh) signals through ectopic expression of a deleted form of the Hh receptor Patched (Ptc), termed Ptc...

journal_title：Molecular cell

authors： Briscoe J,Chen Y,Jessell TM,Struhl G

更新日期：2001-06-01 00:00:00

abstract：:DNA lesions caused by UV damage are thought to be repaired solely by the nucleotide excision repair (NER) pathway in human cells. Patients carrying mutations within genes functioning in this pathway display a range of pathologies, including an increased susceptibility to cancer, premature aging, and neurological defec...

journal_title：Molecular cell

authors： Mazouzi A,Battistini F,Moser SC,Ferreira da Silva J,Wiedner M,Owusu M,Lardeau CH,Ringler A,Weil B,Neesen J,Orozco M,Kubicek S,Loizou JI

更新日期：2017-11-16 00:00:00

abstract：:The protein kinase B-RAF is mutated in approximately 7% of human cancers. Most mutations are activating, but, surprisingly, a small number have reduced kinase activity. However, the latter can still stimulate cellular signaling through the MEK-ERK pathway because they activate the related family member C-RAF. We exami...

journal_title：Molecular cell

authors： Garnett MJ,Rana S,Paterson H,Barford D,Marais R

更新日期：2005-12-22 00:00:00

abstract：:Prenylated Rab proteins exist in the cytosol as soluble, high-affinity complexes with GDI that need to be disrupted for membrane attachment and targeting of Rab proteins. The Legionella pneumophila protein DrrA displaces GDI from Rab1:GDI complexes, incorporating Rab1 into Legionella-containing vacuoles and activating...

journal_title：Molecular cell

authors： Schoebel S,Oesterlin LK,Blankenfeldt W,Goody RS,Itzen A

更新日期：2009-12-25 00:00:00