Insights into the influence of nucleotides on actin family proteins from seven structures of Arp2/3 complex.

abstract：:Two cyclin-dependent kinases have been identified in yeast and mammalian RNA polymerase II transcription initiation complexes. We find that the two yeast kinases are indistinguishable in their ability to phosphorylate the RNA polymerase II CTD, and yet in living cells one kinase is a positive regulator and the other a...

journal_title：Molecular cell

authors： Hengartner CJ,Myer VE,Liao SM,Wilson CJ,Koh SS,Young RA

更新日期：1998-07-01 00:00:00

abstract：:The RNA modification N6-methyladenosine (m6A) modulates mRNA fate and thus affects many biological processes. We analyzed m6A across the transcriptome following infection by dengue virus (DENV), Zika virus (ZIKV), West Nile virus (WNV), and hepatitis C virus (HCV). We found that infection by these viruses in the Flavi...

journal_title：Molecular cell

authors： Gokhale NS,McIntyre ABR,Mattocks MD,Holley CL,Lazear HM,Mason CE,Horner SM

更新日期：2020-02-06 00:00:00

abstract：:The Drosophila gene vasa (vas) encodes an RNA-binding protein required for embryonic patterning and germ cell specification. In vas mutants, translation of several germline mRNAs is reduced. Here we show that VAS interacts directly with the Drosophila homolog of yeast translation initiation factor 2, encoded by a nove...

journal_title：Molecular cell

authors： Carrera P,Johnstone O,Nakamura A,Casanova J,Jäckle H,Lasko P

更新日期：2000-01-01 00:00:00

abstract：:The carboxy-terminal domain (CTD) of the large subunit of RNA polymerase II (Pol II) comprises multiple heptapeptide repeats of the consensus Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Reversible phosphorylation of Ser2, Ser5, and Ser7 during the transcription cycle mediates the sequential recruitment of transcription/RNA pr...

journal_title：Molecular cell

authors： Egloff S,Zaborowska J,Laitem C,Kiss T,Murphy S

更新日期：2012-01-13 00:00:00

abstract：:CRISPR-Cas12a (Cpf1) is an RNA-guided DNA-cutting nuclease that has been repurposed for genome editing. Upon target DNA binding, Cas12a cleaves both the target DNA in cis and non-target single-stranded DNAs (ssDNAs) in trans. To elucidate the molecular basis for both DNase cleavage modes, we performed structural and b...

journal_title：Molecular cell

authors： Swarts DC,Jinek M

更新日期：2019-02-07 00:00:00

abstract：:The RhoA GTPase controls many cellular functions, including gene transcription and actin polymerization. Several lines of evidence suggest that Rho GTPases are required for B cell receptor (BCR) signaling, but whether RhoA is necessary has not been investigated. Here, we show that RhoA is activated, downstream of PI3K...

journal_title：Molecular cell

authors： Saci A,Carpenter CL

更新日期：2005-01-21 00:00:00

abstract：:Study of mutant phenotypes is a fundamental method for understanding gene function. The construction of a near-complete collection of yeast knockouts (YKO) and the unique molecular barcodes (or TAGs) that identify each strain has enabled quantitative functional profiling of Saccharomyces cerevisiae. By using these TAG...

journal_title：Molecular cell

authors： Pan X,Yuan DS,Xiang D,Wang X,Sookhai-Mahadeo S,Bader JS,Hieter P,Spencer F,Boeke JD

更新日期：2004-11-05 00:00:00

abstract：:Unscheduled RNA-DNA hybrids promote the formation DNA double-strand breaks (DSBs) through poorly characterized mechanism. In this issue, Sollier et al. (2014) show that R-loops are processed by XPF and XPG, two structure-specific endonucleases of the NER pathway, to generate DSBs. ...

journal_title：Molecular cell

authors： Lin YL,Pasero P

更新日期：2014-12-18 00:00:00

abstract：:The interaction of transcription factors with target genes is highly dynamic. Whether the dynamic nature of these interactions is merely an intrinsic property of transcription factors or serves a regulatory role is unknown. Here we have used single-cell fluorescence imaging combined with computational modeling and chr...

journal_title：Molecular cell

authors： Gorski SA,Snyder SK,John S,Grummt I,Misteli T

更新日期：2008-05-23 00:00:00

abstract：:AAA+ proteins (ATPases associated with various cellular activities) are oligomeric ATPases that use ATP hydrolysis to remodel their substrates. By similarity with GTPases, a dynamic organization of the nucleotide-binding pockets between ATPase protomers is proposed to regulate functionality. Using the transcription ac...

journal_title：Molecular cell

authors： Joly N,Zhang N,Buck M

更新日期：2012-08-10 00:00:00

abstract：:AMPK is a highly conserved sensor of cellular energy status that is activated under conditions of low intracellular ATP. AMPK responds to energy stress by suppressing cell growth and biosynthetic processes, in part through its inhibition of the rapamycin-sensitive mTOR (mTORC1) pathway. AMPK phosphorylation of the TSC...

journal_title：Molecular cell

authors： Gwinn DM,Shackelford DB,Egan DF,Mihaylova MM,Mery A,Vasquez DS,Turk BE,Shaw RJ

更新日期：2008-04-25 00:00:00

abstract：:2'3'-cyclic-GMP-AMP (cGAMP) is a second messenger that activates the antiviral stimulator of interferon genes (STING) pathway. We recently identified a novel role for cGAMP as a soluble, extracellular immunotransmitter that is produced and secreted by cancer cells. Secreted cGAMP is then sensed by host cells, elicitin...

journal_title：Molecular cell

authors： Ritchie C,Cordova AF,Hess GT,Bassik MC,Li L

更新日期：2019-07-25 00:00:00

abstract：:Messenger RNAs with premature translation termination codons (PTCs) are degraded by nonsense-mediated mRNA decay (NMD). In mammals, PTCs are discriminated from physiological stop codons by a process thought to involve the splicing-dependent deposition of an exon junction complex (EJC), EJC-mediated recruitment of Upf3...

journal_title：Molecular cell

authors： Gehring NH,Neu-Yilik G,Schell T,Hentze MW,Kulozik AE

更新日期：2003-04-01 00:00:00

abstract：:In eukaryotes, tRNAs are synthesized in the nucleus and after several maturation steps exported to the cytoplasm. Here, we identify exportin-t as a specific mediator of tRNA export. It is a RanGTP-binding, importin beta-related factor with predominantly nuclear localization. It shuttles rapidly between nucleus and cyt...

journal_title：Molecular cell

authors： Kutay U,Lipowsky G,Izaurralde E,Bischoff FR,Schwarzmaier P,Hartmann E,Görlich D

更新日期：1998-02-01 00:00:00

abstract：:Errors in translation cause cytotoxic protein misfolding and aggregation. In this issue, Ling et al. (2012) show that scavenging or suppression of reactive oxygen species in E. coli reduces the cellular costs of error-induced aggregation. ...

journal_title：Molecular cell

authors： Drummond DA

更新日期：2012-12-14 00:00:00

abstract：:Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins constitute an adaptive immune system in prokaryotes. The system preserves memories of prior infections by integrating short segments of foreign DNA, termed spacers, into the CRISPR array in a process termed adaptati...

journal_title：Molecular cell

authors： Sternberg SH,Richter H,Charpentier E,Qimron U

更新日期：2016-03-17 00:00:00

abstract：:In this issue, Randow and coworkers (von Muhlinen et al., 2012) report that LC3C plays an essential role in Salmonella clearance by interacting with the autophagic receptor NDP52, with consequent recruitment of all other ATG8 proteins to assist in the buildup of the autophagic membrane. ...

journal_title：Molecular cell

authors： Shpilka T,Elazar Z

更新日期：2012-11-09 00:00:00

abstract：:In this issue of Molecular Cell, Guo et al. (2014) report that misfolded or aggregated nuclear proteins, such as pathogenic polyQ proteins, are cleared by a SUMO-dependent quality control pathway, which involves the E3 SUMO ligase PML and the SUMO-targeted ubiquitin ligase RNF4. ...

journal_title：Molecular cell

authors： Gärtner A,Muller S

更新日期：2014-07-03 00:00:00

abstract：:We used DNA microarrays to profile gene expression patterns in the C. elegans germline and identified 1416 germline-enriched transcripts that define three groups. The sperm-enriched group contains an unusually large number of protein kinases and phosphatases. The oocyte-enriched group includes potentially new componen...

journal_title：Molecular cell

authors： Reinke V,Smith HE,Nance J,Wang J,Van Doren C,Begley R,Jones SJ,Davis EB,Scherer S,Ward S,Kim SK

更新日期：2000-09-01 00:00:00

abstract：:MicroRNAs (miRNAs) inhibit mRNA expression in general by base pairing to the 3'UTR of target mRNAs and consequently inhibiting translation and/or initiating poly(A) tail deadenylation and mRNA destabilization. Here we examine the mechanism and kinetics of miRNA-mediated deadenylation in mouse Krebs-2 ascites extract. ...

journal_title：Molecular cell

authors： Fabian MR,Mathonnet G,Sundermeier T,Mathys H,Zipprich JT,Svitkin YV,Rivas F,Jinek M,Wohlschlegel J,Doudna JA,Chen CY,Shyu AB,Yates JR 3rd,Hannon GJ,Filipowicz W,Duchaine TF,Sonenberg N

更新日期：2009-09-24 00:00:00

abstract：:Argonaute proteins are the mediators of small RNA-guided gene silencing pathways. In this issue, Couvillion and coworkers (Couvillion et al., 2012) found an unexpected function for a Tetrahymena Argonaute protein: It forms a complex with tRNA fragments and is required for nuclear Xrn2 localization and function. ...

journal_title：Molecular cell

authors： Hasler D,Meister G

更新日期：2012-11-30 00:00:00

abstract：:Neurons undertake an amazing journey from the center of the developing mammalian brain to the outer layers of the cerebral cortex. Doublecortin, a component of the microtubule cytoskeleton, is essential in postmitotic neurons and was identified because its mutation disrupts human brain development. Doublecortin stabil...

journal_title：Molecular cell

authors： Moores CA,Perderiset M,Francis F,Chelly J,Houdusse A,Milligan RA

更新日期：2004-06-18 00:00:00

abstract：:MRE11 and RAD50 are known to be required for nonhomologous joining of DNA ends in vivo. We have investigated the enzymatic activities of the purified proteins and found that Mre11 by itself has 3' to 5' exonuclease activity that is increased when Mre11 is in a complex with Rad50. Mre11 also exhibits endonuclease activ...

journal_title：Molecular cell

authors： Paull TT,Gellert M

更新日期：1998-06-01 00:00:00

abstract：:A variety of debilitating diseases including diabetes, Alzheimer's, Huntington's, Parkinson's, and prion-based diseases are linked to stress within the endoplasmic reticulum (ER). Using S. cerevisiae, we sought to determine the relationship between protein misfolding, ER stress, and cell death. In the absence of ERV29...

journal_title：Molecular cell

authors： Haynes CM,Titus EA,Cooper AA

更新日期：2004-09-10 00:00:00

abstract：:The mechanisms controlling growth and patterning along the proximal-distal axis of the vertebrate limb are yet to be understood. We show that restriction of expression of the homeobox gene Meis2 to proximal regions of the limb bud is essential for limb development, since ectopic Meis2 severely disrupts limb outgrowth....

journal_title：Molecular cell

authors： Capdevila J,Tsukui T,Rodríquez Esteban C,Zappavigna V,Izpisúa Belmonte JC

更新日期：1999-11-01 00:00:00

abstract：:The PTEN tumor suppressor controls cell death and survival by regulating functions of various molecular targets. While the role of PTEN lipid-phosphatase activity on PtdIns(3,4,5)P3 and inhibition of PI3K pathway is well characterized, the biological relevance of PTEN protein-phosphatase activity remains undefined. He...

journal_title：Molecular cell

authors： Yip HYK,Chee A,Ang CS,Shin SY,Ooms LM,Mohammadi Z,Phillips WA,Daly RJ,Cole TJ,Bronson RT,Nguyen LK,Tiganis T,Hobbs RM,McLean CA,Mitchell CA,Papa A

更新日期：2020-10-15 00:00:00

abstract：:The Chk2 Ser/Thr kinase plays crucial, evolutionarily conserved roles in cellular responses to DNA damage. Identification of two pro-oncogenic mutations within the Chk2 FHA domain has highlighted its importance for Chk2 function in checkpoint activation. The X-ray structure of the Chk2 FHA domain in complex with an in...

journal_title：Molecular cell

authors： Li J,Williams BL,Haire LF,Goldberg M,Wilker E,Durocher D,Yaffe MB,Jackson SP,Smerdon SJ

更新日期：2002-05-01 00:00:00

abstract：:Zinc is an essential trace element, and impaired zinc homeostasis is implicated in the pathogenesis of various human diseases. However, the mechanisms cells use to respond to zinc deficiency are poorly understood. We previously reported that amyotrophic lateral sclerosis (ALS)-linked pathogenic mutants of SOD1 cause c...

journal_title：Molecular cell

authors： Homma K,Fujisawa T,Tsuburaya N,Yamaguchi N,Kadowaki H,Takeda K,Nishitoh H,Matsuzawa A,Naguro I,Ichijo H

更新日期：2013-10-10 00:00:00

abstract：:Proliferating metazoan cells respond to damage that has the potential to cause genomic instability by restricting the cell division cycle or by initiating apoptosis. The molecular mechanisms determining the balance between these responses are not well understood. Here, we show that the apoptotic initiator protease cas...

journal_title：Molecular cell

authors： Allan LA,Clarke PR

更新日期：2007-04-27 00:00:00

abstract：:Double-strand breaks (DSBs) of DNA in eukaryotic cells are predominantly repaired by non-homologous end joining (NHEJ). The histone chaperone anti-silencing factor 1a (ASF1a) interacts with MDC1 and is recruited to sites of DSBs to facilitate the interaction of phospho-ATM with MDC1 and phosphorylation of MDC1, which ...

journal_title：Molecular cell

authors： Lee KY,Im JS,Shibata E,Dutta A

更新日期：2017-10-05 00:00:00