Abstract：
:The RSC chromatin remodeler slides and ejects nucleosomes, utilizing a catalytic subunit (Sth1) with DNA translocation activity, which can pump DNA around the nucleosome. A central question is whether and how DNA translocation is regulated to achieve sliding versus ejection. Here, we report the regulation of DNA translocation efficiency by two domains residing on Sth1 (Post-HSA and Protrusion 1) and by actin-related proteins (ARPs) that bind Sth1. ARPs facilitated sliding and ejection by improving "coupling"-the amount of DNA translocation by Sth1 relative to ATP hydrolysis. We also identified and characterized Protrusion 1 mutations that promote "coupling," and Post-HSA mutations that improve ATP hydrolysis; notably, the strongest mutations conferred efficient nucleosome ejection without ARPs. Taken together, sliding-to-ejection involves a continuum of DNA translocation efficiency, consistent with higher magnitudes of ATPase and coupling activities (involving ARPs and Sth1 domains), enabling the simultaneous rupture of multiple histone-DNA contacts facilitating ejection.
journal_name
Mol Celljournal_title
Molecular cellauthors
Clapier CR,Kasten MM,Parnell TJ,Viswanathan R,Szerlong H,Sirinakis G,Zhang Y,Cairns BRdoi
10.1016/j.molcel.2016.03.032subject
Has Abstractpub_date
2016-05-05 00:00:00pages
453-461issue
3eissn
1097-2765issn
1097-4164pii
S1097-2765(16)30015-6journal_volume
62pub_type
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