Negative regulation of the E3 ubiquitin ligase itch via Fyn-mediated tyrosine phosphorylation.

abstract：:The splitting of chromosomes in anaphase and their delivery into the daughter cells needs to be accurately executed to maintain genome stability. Chromosome splitting requires the degradation of securin, whereas the distribution of the chromosomes into the daughter cells requires the degradation of cyclin B. We show t...

journal_title：Molecular cell

authors： Kamenz J,Mihaljev T,Kubis A,Legewie S,Hauf S

更新日期：2015-11-05 00:00:00

abstract：:In this issue of Molecular Cell, Gross et al. (2016) find a CpG DNA methylation signature in blood cells of patients with chronic well-controlled HIV infection that correlates with accelerated aging. ...

journal_title：Molecular cell

authors： Boulias K,Lieberman J,Greer EL

更新日期：2016-04-21 00:00:00

abstract：:Faithful duplication of the genome in S phase followed by its accurate segregation in mitosis is essential to maintain genomic integrity. Recent studies have suggested that proteins involved in DNA transactions are also required for whole-chromosome stability. Here we demonstrate that the MRN (Mre11, Rad50, and Nbs1) ...

journal_title：Molecular cell

authors： Rozier L,Guo Y,Peterson S,Sato M,Baer R,Gautier J,Mao Y

更新日期：2013-03-28 00:00:00

abstract：:AAA+ proteins (ATPases associated with various cellular activities) are oligomeric ATPases that use ATP hydrolysis to remodel their substrates. By similarity with GTPases, a dynamic organization of the nucleotide-binding pockets between ATPase protomers is proposed to regulate functionality. Using the transcription ac...

journal_title：Molecular cell

authors： Joly N,Zhang N,Buck M

更新日期：2012-08-10 00:00:00

abstract：:Chaperone-mediated autophagy (CMA) is involved in cellular quality control, and in the response to stress, deterioration in CMA contributes to the aging phenotype and to various disorders. The paper by Bandyopadhyay et al. in this issue of Molecular Cell demonstrates the role of GTP binding by elongation factor 1 alph...

journal_title：Molecular cell

authors： Beau I,Codogno P

更新日期：2010-08-27 00:00:00

abstract：:Most colorectal cancers (CRCs) containing activated BRAF (BRAF[V600E]) have a CpG island methylator phenotype (CIMP) characterized by aberrant hypermethylation of many genes, including the mismatch repair gene MLH1. MLH1 silencing results in microsatellite instability and a hypermutable phenotype. Through an RNAi scre...

journal_title：Molecular cell

authors： Fang M,Ou J,Hutchinson L,Green MR

更新日期：2014-09-18 00:00:00

abstract：:In Parkinson's disease (PD), α-synuclein (αS) pathologically impacts the brain, a highly lipid-rich organ. We investigated how alterations in αS or lipid/fatty acid homeostasis affect each other. Lipidomic profiling of human αS-expressing yeast revealed increases in oleic acid (OA, 18:1), diglycerides, and triglycerid...

journal_title：Molecular cell

authors： Fanning S,Haque A,Imberdis T,Baru V,Barrasa MI,Nuber S,Termine D,Ramalingam N,Ho GPH,Noble T,Sandoe J,Lou Y,Landgraf D,Freyzon Y,Newby G,Soldner F,Terry-Kantor E,Kim TE,Hofbauer HF,Becuwe M,Jaenisch R,Pincus D,

更新日期：2019-03-07 00:00:00

abstract：:The response to endoplasmic reticulum (ER) stress relies on activation of unfolded protein response (UPR) sensors, and the outcome of the UPR depends on the duration and strength of signal. Here, we demonstrate a mechanism that attenuates the activity of the UPR sensor inositol-requiring enzyme 1α (IRE1α). A resident ...

journal_title：Molecular cell

authors： Eletto D,Eletto D,Dersh D,Gidalevitz T,Argon Y

更新日期：2014-02-20 00:00:00

abstract：:Siz1 is a founding member of the Siz/PIAS RING family of SUMO E3 ligases. The X-ray structure of an active Siz1 ligase revealed an elongated tripartite architecture comprised of an N-terminal PINIT domain, a central zinc-containing RING-like SP-RING domain, and a C-terminal domain we term the SP-CTD. Structure-based m...

journal_title：Molecular cell

authors： Yunus AA,Lima CD

更新日期：2009-09-11 00:00:00

abstract：:The control of mRNA stability plays a central role in regulating gene expression. In metazoans, the earliest stages of development are driven by maternally supplied mRNAs. The degradation of these maternal mRNAs is critical for promoting the maternal-to-zygotic transition of developmental programs, although the underl...

journal_title：Molecular cell

authors： Mishima Y,Tomari Y

更新日期：2016-03-17 00:00:00

abstract：:Loss of endogenous tumor suppression is a critical step on the road to cancer. In a recent paper in Molecular Cell, Wang and colleagues provide evidence that inactivation of the pivotal BRCA1 tumor suppressor disrupts a safeguard gene network that opposes cell proliferation and cell survival. ...

journal_title：Molecular cell

authors： Luo J,Altieri DC

更新日期：2008-10-24 00:00:00

abstract：:Gcn4 is a yeast transcriptional activator induced by amino acid starvation. ChIP-seq analysis revealed 546 genomic sites occupied by Gcn4 in starved cells, representing ∼30% of Gcn4-binding motifs. Surprisingly, only ∼40% of the bound sites are in promoters, of which only ∼60% activate transcription, indicating extens...

journal_title：Molecular cell

authors： Rawal Y,Chereji RV,Valabhoju V,Qiu H,Ocampo J,Clark DJ,Hinnebusch AG

更新日期：2018-04-19 00:00:00

abstract：:One of the earliest steps in metazoan pre-mRNA splicing involves binding of U2 snRNP auxiliary factor (U2AF) 65 KDa subunit to the polypyrimidine (Py) tract and of the 35 KDa subunit to the invariant AG dinucleotide at the intron 3' end. Here we use in vitro and in vivo depletion, as well as reconstitution assays usin...

journal_title：Molecular cell

authors： Tavanez JP,Madl T,Kooshapur H,Sattler M,Valcárcel J

更新日期：2012-02-10 00:00:00

abstract：:Polymerase gamma, which replicates and repairs mitochondrial DNA, requires the Pol gamma B subunit for processivity. We determined the crystal structure of mouse Pol gamma B, a core component of the mitochondrial replication machinery. Pol gamma B shows high similarity to glycyl-tRNA synthetase and dimerizes through a...

journal_title：Molecular cell

authors： Carrodeguas JA,Theis K,Bogenhagen DF,Kisker C

更新日期：2001-01-01 00:00:00

abstract：:Glaucoma, a blinding neurodegenerative disease, whose risk factors include elevated intraocular pressure (IOP), age, and genetics, is characterized by accelerated and progressive retinal ganglion cell (RGC) death. Despite decades of research, the mechanism of RGC death in glaucoma is still unknown. Here, we demonstrat...

journal_title：Molecular cell

authors： Skowronska-Krawczyk D,Zhao L,Zhu J,Weinreb RN,Cao G,Luo J,Flagg K,Patel S,Wen C,Krupa M,Luo H,Ouyang H,Lin D,Wang W,Li G,Xu Y,Li O,Chung C,Yeh E,Jafari M,Ai M,Zhong Z,Shi W,Zheng L,Krawczyk M,Chen D,Sh

更新日期：2015-09-17 00:00:00

abstract：:The mechanisms contributing to transcription-associated genomic instability are both complex and incompletely understood. Although R-loops are normal transcriptional intermediates, they are also associated with genomic instability. Here, we show that BRCA1 is recruited to R-loops that form normally over a subset of tr...

journal_title：Molecular cell

authors： Hatchi E,Skourti-Stathaki K,Ventz S,Pinello L,Yen A,Kamieniarz-Gdula K,Dimitrov S,Pathania S,McKinney KM,Eaton ML,Kellis M,Hill SJ,Parmigiani G,Proudfoot NJ,Livingston DM

更新日期：2015-02-19 00:00:00

abstract：:The Fanconi anemia (FA) pathway is implicated in DNA repair and cancer predisposition. Central to this pathway is the FA core complex, which is targeted to chromatin by FANCM and FAAP24 following replication stress. Here we show that FANCM and FAAP24 interact with the checkpoint protein HCLK2 independently of the FA c...

journal_title：Molecular cell

authors： Collis SJ,Ciccia A,Deans AJ,Horejsí Z,Martin JS,Maslen SL,Skehel JM,Elledge SJ,West SC,Boulton SJ

更新日期：2008-11-07 00:00:00

abstract：:In Xer site-specific recombination, sequential DNA strand exchange reactions are catalyzed by a heterotetrameric complex composed of two recombinases, XerC and XerD. It is demonstrated that XerC and XerD catalytic activity is controlled by an interaction involving the C-terminal end of each protein (the donor region) ...

journal_title：Molecular cell

authors： Hallet B,Arciszewska LK,Sherratt DJ

更新日期：1999-12-01 00:00:00

abstract：:Replication forks frequently break and must be repaired by recombination. A reconstituted reaction now allows the factors that coordinate conversion from a recombination intermediate back to a replication fork to be defined. The PriA protein plays a key role in this control. ...

journal_title：Molecular cell

authors： Lovett ST

更新日期：2003-03-01 00:00:00

abstract：:Nucleotide-binding site leucine-rich repeat (NLR) receptors perceive pathogen effectors and trigger plant immunity. However, the mechanisms underlying NLR-triggered defense responses remain obscure. The recently discovered Pigm locus in rice encodes a cluster of NLRs, including PigmR, which confers broad-spectrum resi...

journal_title：Molecular cell

authors： Zhai K,Deng Y,Liang D,Tang J,Liu J,Yan B,Yin X,Lin H,Chen F,Yang D,Xie Z,Liu JY,Li Q,Zhang L,He Z

更新日期：2019-06-06 00:00:00

abstract：:RING ubiquitin ligases (E3) recruit ubiquitin-conjugate enzymes (E2) charged with ubiquitin (Ub) to catalyze ubiquitination. Non-covalent Ub binding to the backside of certain E2s promotes processive polyUb formation, but the mechanism remains elusive. Here, we show that backside bound Ub (Ub(B)) enhances both RING-in...

journal_title：Molecular cell

authors： Buetow L,Gabrielsen M,Anthony NG,Dou H,Patel A,Aitkenhead H,Sibbet GJ,Smith BO,Huang DT

更新日期：2015-04-16 00:00:00

abstract：:The SMN complex assembles Sm cores on snRNAs, a key step in the biogenesis of snRNPs, the spliceosome's major components. Here, using SMN complex inhibitors identified by high-throughput screening and a ribo-proteomic strategy on formaldehyde crosslinked RNPs, we dissected this pathway in cells. We show that protein s...

journal_title：Molecular cell

authors： Yong J,Kasim M,Bachorik JL,Wan L,Dreyfuss G

更新日期：2010-05-28 00:00:00

abstract：:Early endocytic membrane traffic is regulated by the small GTPase Rab5, which cycles between GTP- and GDP-bound states as well as between membrane and cytosol. The latter cycle depends on GDI, which functions as a Rab vehicle in the aqueous environment of the cytosol. Here, we report that formation of the GDI:Rab5 com...

journal_title：Molecular cell

authors： Cavalli V,Vilbois F,Corti M,Marcote MJ,Tamura K,Karin M,Arkinstall S,Gruenberg J

更新日期：2001-02-01 00:00:00

abstract：:Members of the ISWI family of chromatin remodeling factors exhibit ATP-dependent nucleosome sliding, loading, and spacing activities in vitro. However, it is unclear which of these activities are utilized by ISWI complexes to remodel chromatin in vivo. We therefore sought to identify the mechanisms of chromatin remode...

journal_title：Molecular cell

authors： Fazzio TG,Tsukiyama T

更新日期：2003-11-01 00:00:00

abstract：:Cancer cells acquire unlimited proliferative capacity by either re-expressing telomerase or inducing alternative lengthening of telomeres (ALT), which relies on telomere recombination. Here, we show that ALT recombination requires coordinate regulation of the SMX and BTR complexes to ensure the appropriate balance of ...

journal_title：Molecular cell

authors： Panier S,Maric M,Hewitt G,Mason-Osann E,Gali H,Dai A,Labadorf A,Guervilly JH,Ruis P,Segura-Bayona S,Belan O,Marzec P,Gaillard PL,Flynn RL,Boulton SJ

更新日期：2019-10-03 00:00:00

abstract：:Elongation factor G (EF-G) catalyzes tRNA translocation on the ribosome. Here a cryo-EM reconstruction of the 70S*EF-G ribosomal complex at 7.3 A resolution and the crystal structure of EF-G-2*GTP, an EF-G homolog, at 2.2 A resolution are presented. EF-G-2*GTP is structurally distinct from previous EF-G structures, an...

journal_title：Molecular cell

authors： Connell SR,Takemoto C,Wilson DN,Wang H,Murayama K,Terada T,Shirouzu M,Rost M,Schüler M,Giesebrecht J,Dabrowski M,Mielke T,Fucini P,Yokoyama S,Spahn CM

更新日期：2007-03-09 00:00:00

abstract：:Metazoan histone mRNAs end in a highly conserved stem-loop structure followed by ACCCA. Previous studies have suggested that the stem-loop binding protein (SLBP) is the only protein binding this region. Using RNA affinity purification, we identified a second protein, designated 3'hExo, that contains a SAP and a 3' exo...

journal_title：Molecular cell

authors： Dominski Z,Yang XC,Kaygun H,Dadlez M,Marzluff WF

更新日期：2003-08-01 00:00:00

abstract：:In this issue of Molecular Cell,Sharma et al. (2019) show that normal cell growth requires conversion of an arginine residue in the RNA polymerase II C-terminal domain (CTD) to citrulline, uncovering a potential regulatory pathway involving opposing arginine modifications. ...

journal_title：Molecular cell

authors： Corden JL

更新日期：2019-01-03 00:00:00

abstract：:Identifying DNA fragile sites is crucial to reveal hotspots of genomic rearrangements, yet their precise mapping has been a challenge. A new study in this issue of Molecular Cell (Canela et al., 2016) introduces a highly sensitive and accurate method to detect DNA breaks in vivo that can be adapted to various experime...

journal_title：Molecular cell

authors： Banerjee U,Soutoglou E

更新日期：2016-09-01 00:00:00

abstract：:The formation of mRNPs controls the interaction of the translation and degradation machinery with individual mRNAs. The yeast Scd6 protein and its orthologs regulate translation and mRNA degradation in yeast, C. elegans, D. melanogaster, and humans by an unknown mechanism. We demonstrate that Scd6 represses translatio...

journal_title：Molecular cell

authors： Rajyaguru P,She M,Parker R

更新日期：2012-01-27 00:00:00