Abstract：
:The essential histone H3 lysine 79 methyltransferase Dot1L regulates transcription and genomic stability and is deregulated in leukemia. The activity of Dot1L is stimulated by mono-ubiquitination of histone H2B on lysine 120 (H2BK120Ub); however, the detailed mechanism is not understood. We report cryo-EM structures of human Dot1L bound to (1) H2BK120Ub and (2) unmodified nucleosome substrates at 3.5 Å and 4.9 Å, respectively. Comparison of both structures, complemented with biochemical experiments, provides critical insights into the mechanism of Dot1L stimulation by H2BK120Ub. Both structures show Dot1L binding to the same extended surface of the histone octamer. In yeast, this surface is used by silencing proteins involved in heterochromatin formation, explaining the mechanism of their competition with Dot1. These results provide a strong foundation for understanding conserved crosstalk between histone modifications found at actively transcribed genes and offer a general model of how ubiquitin might regulate the activity of chromatin enzymes.
journal_name
Mol Celljournal_title
Molecular cellauthors
Valencia-Sánchez MI,De Ioannes P,Wang M,Vasilyev N,Chen R,Nudler E,Armache JP,Armache KJdoi
10.1016/j.molcel.2019.03.029subject
Has Abstractpub_date
2019-06-06 00:00:00pages
1010-1019.e6issue
5eissn
1097-2765issn
1097-4164pii
S1097-2765(19)30233-3journal_volume
74pub_type
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