Generating crossovers by resolution of nicked Holliday junctions: a role for Mus81-Eme1 in meiosis.

Abstract:

:The double Holliday junction (dHJ) is generally regarded to be a key intermediate of meiotic recombination, whose resolution is critical for the formation of crossover recombinants. In fission yeast, the Mus81-Eme1 endonuclease has been implicated in resolving dHJs. Consistent with this role, we show that Mus81-Eme1 is required for generating meiotic crossovers. However, purified Mus81-Eme1 prefers to cleave junctions that mimic those formed during the transition from double-strand break to dHJ. Crucially, these junctions are cleaved by Mus81-Eme1 in precisely the right orientation to guarantee the formation of a crossover every time. These data demonstrate how crossovers could arise without forming or resolving dHJs using an enzyme that is widely conserved amongst eukaryotes.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Osman F,Dixon J,Doe CL,Whitby MC

doi

10.1016/s1097-2765(03)00343-5

subject

Has Abstract

pub_date

2003-09-01 00:00:00

pages

761-74

issue

3

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(03)00343-5

journal_volume

12

pub_type

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