Methyl-Metabolite Depletion Elicits Adaptive Responses to Support Heterochromatin Stability and Epigenetic Persistence.

abstract：:A defining feature of centromeres is the presence of the histone H3 variant CENP-A(Cnp1). It is not known how CENP-A(Cnp1) is specifically delivered to, and assembled into, centromeric chromatin. Through a screen for factors involved in kinetochore integrity in fission yeast, we identified Sim3. Sim3 is homologous to ...

journal_title：Molecular cell

authors： Dunleavy EM,Pidoux AL,Monet M,Bonilla C,Richardson W,Hamilton GL,Ekwall K,McLaughlin PJ,Allshire RC

更新日期：2007-12-28 00:00:00

abstract：:Multisite phosphorylation of kinases can induce on-off or graded regulation of catalytic activity; however, its influence on substrate specificity remains unclear. Here, we show that multisite phosphorylation of ribosomal protein S6 kinase 1 (S6K1) alters target selection. Agonist-inducible phosphorylation of glutamyl...

journal_title：Molecular cell

authors： Arif A,Jia J,Willard B,Li X,Fox PL

更新日期：2019-02-07 00:00:00

abstract：:The adenomatous polyposis coli (APC) tumor-suppressor protein, together with Axin and GSK3beta, forms a Wnt-regulated signaling complex that mediates phosphorylation-dependent degradation of beta-catenin by the proteasome. Siah-1, the human homolog of Drosophila seven in absentia, is a p53-inducible mediator of cell c...

journal_title：Molecular cell

authors： Liu J,Stevens J,Rote CA,Yost HJ,Hu Y,Neufeld KL,White RL,Matsunami N

更新日期：2001-05-01 00:00:00

abstract：:Melanoma and other cancers harbor oncogenic mutations in the protein kinase B-Raf, which leads to constitutive activation and dysregulation of MAP kinase signaling. In order to elucidate molecular determinants responsible for B-Raf control of cancer phenotypes, we present a method for phosphoprotein profiling, using n...

journal_title：Molecular cell

authors： Old WM,Shabb JB,Houel S,Wang H,Couts KL,Yen CY,Litman ES,Croy CH,Meyer-Arendt K,Miranda JG,Brown RA,Witze ES,Schweppe RE,Resing KA,Ahn NG

更新日期：2009-04-10 00:00:00

abstract：:Elongation factor G (EF-G) catalyzes tRNA translocation on the ribosome. Here a cryo-EM reconstruction of the 70S*EF-G ribosomal complex at 7.3 A resolution and the crystal structure of EF-G-2*GTP, an EF-G homolog, at 2.2 A resolution are presented. EF-G-2*GTP is structurally distinct from previous EF-G structures, an...

journal_title：Molecular cell

authors： Connell SR,Takemoto C,Wilson DN,Wang H,Murayama K,Terada T,Shirouzu M,Rost M,Schüler M,Giesebrecht J,Dabrowski M,Mielke T,Fucini P,Yokoyama S,Spahn CM

更新日期：2007-03-09 00:00:00

abstract：:Polymerase gamma, which replicates and repairs mitochondrial DNA, requires the Pol gamma B subunit for processivity. We determined the crystal structure of mouse Pol gamma B, a core component of the mitochondrial replication machinery. Pol gamma B shows high similarity to glycyl-tRNA synthetase and dimerizes through a...

journal_title：Molecular cell

authors： Carrodeguas JA,Theis K,Bogenhagen DF,Kisker C

更新日期：2001-01-01 00:00:00

abstract：:In this issue of Molecular Cell, Chen et al. (2014) provide evidence that FMRP represses translation by binding the ribosome, suggesting a novel form of translational control. ...

journal_title：Molecular cell

authors： Harigaya Y,Parker R

更新日期：2014-05-08 00:00:00

abstract：:The DNA polymerase from phage phi29 is a B family polymerase that initiates replication using a protein as a primer, attaching the first nucleotide of the phage genome to the hydroxyl of a specific serine of the priming protein. The crystal structure of phi29 DNA polymerase determined at 2.2 A resolution provides expl...

journal_title：Molecular cell

authors： Kamtekar S,Berman AJ,Wang J,Lázaro JM,de Vega M,Blanco L,Salas M,Steitz TA

更新日期：2004-11-19 00:00:00

abstract：:Upon heterologous overexpression, many proteins misfold or aggregate, thus resulting in low functional yields. Human acetylcholinesterase (hAChE), an enzyme mediating synaptic transmission, is a typical case of a human protein that necessitates mammalian systems to obtain functional expression. We developed a computat...

journal_title：Molecular cell

authors： Goldenzweig A,Goldsmith M,Hill SE,Gertman O,Laurino P,Ashani Y,Dym O,Unger T,Albeck S,Prilusky J,Lieberman RL,Aharoni A,Silman I,Sussman JL,Tawfik DS,Fleishman SJ

更新日期：2016-07-21 00:00:00

abstract：:Genome instability, a hallmark of cancer progression, is thought to arise through DNA double strand breaks (DSBs). Studies in yeast and mammalian cells have shown that DSBs and instability can occur through RNA:DNA hybrids generated by defects in RNA elongation and splicing. We report that in yeast hybrids naturally f...

journal_title：Molecular cell

authors： Wahba L,Amon JD,Koshland D,Vuica-Ross M

更新日期：2011-12-23 00:00:00

abstract：:The histone methyltransferase PR-Set7/Set8 is the sole enzyme that catalyzes monomethylation of histone H4 at K20 (H4K20me1). Previous reports document disparate evidence regarding PR-Set7 expression during the cell cycle, the biological relevance of PR-Set7 interaction with PCNA, and its role in the cell. We find tha...

journal_title：Molecular cell

authors： Oda H,Hübner MR,Beck DB,Vermeulen M,Hurwitz J,Spector DL,Reinberg D

更新日期：2010-11-12 00:00:00

abstract：:The bHLH factors HAND1 and HAND2 are required for heart, vascular, neuronal, limb, and extraembryonic development. Unlike most bHLH proteins, HAND factors exhibit promiscuous dimerization properties. We report that phosphorylation/dephosphorylation via PKA, PKC, and a specific heterotrimeric protein phosphatase 2A (PP...

journal_title：Molecular cell

authors： Firulli BA,Howard MJ,McDaid JR,McIlreavey L,Dionne KM,Centonze VE,Cserjesi P,Virshup DM,Firulli AB

更新日期：2003-11-01 00:00:00

abstract：:In bacteria, RNA polymerase (RNAP) initiates transcription by synthesizing short transcripts that are either released or extended to allow RNAP to escape from the promoter. The mechanism of initial transcription is unclear due to the presence of transient intermediates and molecular heterogeneity. Here, we studied ini...

journal_title：Molecular cell

authors： Duchi D,Bauer DL,Fernandez L,Evans G,Robb N,Hwang LC,Gryte K,Tomescu A,Zawadzki P,Morichaud Z,Brodolin K,Kapanidis AN

更新日期：2016-09-15 00:00:00

abstract：:Faithful propagation of functionally distinct chromatin states is crucial for maintaining cellular identity, and its breakdown can lead to diseases such as cancer. Whereas mechanisms that sustain repressed states have been intensely studied, regulatory circuits that protect active chromatin from inactivating signals a...

journal_title：Molecular cell

authors： Flury V,Georgescu PR,Iesmantavicius V,Shimada Y,Kuzdere T,Braun S,Bühler M

更新日期：2017-07-20 00:00:00

abstract：:Heterochromatin is required to restrict aberrant expression of retrotransposons, but it remains poorly defined due to the underlying repeat-rich sequences. We dissected Suv39h-dependent histone H3 lysine 9 trimethylation (H3K9me3) by genome-wide ChIP sequencing in mouse embryonic stem cells (ESCs). Refined bioinformat...

journal_title：Molecular cell

authors： Bulut-Karslioglu A,De La Rosa-Velázquez IA,Ramirez F,Barenboim M,Onishi-Seebacher M,Arand J,Galán C,Winter GE,Engist B,Gerle B,O'Sullivan RJ,Martens JH,Walter J,Manke T,Lachner M,Jenuwein T

更新日期：2014-07-17 00:00:00

abstract：:Bicoid (BCD), the anterior determinant of Drosophila, controls embryonic gene expression by transcriptional activation and translational repression. Both functions require the homeodomain (HD), which recognizes DNA motifs at target gene enhancers and a specific sequence interval in the 3' untranslated region of caudal...

journal_title：Molecular cell

authors： Niessing D,Driever W,Sprenger F,Taubert H,Jäckle H,Rivera-Pomar R

更新日期：2000-02-01 00:00:00

abstract：:Both EZH2 and NF-κB contribute to aggressive breast cancer, yet whether the two oncogenic factors have functional crosstalk in breast cancer is unknown. Here, we uncover an unexpected role of EZH2 in conferring the constitutive activation of NF-κB target gene expression in ER-negative basal-like breast cancer cells. T...

journal_title：Molecular cell

authors： Lee ST,Li Z,Wu Z,Aau M,Guan P,Karuturi RK,Liou YC,Yu Q

更新日期：2011-09-02 00:00:00

abstract：:RING ubiquitin ligases (E3) recruit ubiquitin-conjugate enzymes (E2) charged with ubiquitin (Ub) to catalyze ubiquitination. Non-covalent Ub binding to the backside of certain E2s promotes processive polyUb formation, but the mechanism remains elusive. Here, we show that backside bound Ub (Ub(B)) enhances both RING-in...

journal_title：Molecular cell

authors： Buetow L,Gabrielsen M,Anthony NG,Dou H,Patel A,Aitkenhead H,Sibbet GJ,Smith BO,Huang DT

更新日期：2015-04-16 00:00:00

abstract：:Protein phase separation by low-complexity, intrinsically disordered domains generates membraneless organelles and links to neurodegeneration. Cellular prion protein (PrPC) contains such domains, causes spongiform degeneration, and is a receptor for Alzheimer's amyloid-β oligomers (Aβo). Here, we show that PrPC separa...

journal_title：Molecular cell

authors： Kostylev MA,Tuttle MD,Lee S,Klein LE,Takahashi H,Cox TO,Gunther EC,Zilm KW,Strittmatter SM

更新日期：2018-11-01 00:00:00

abstract：:Cells constantly adjust their metabolism in response to environmental conditions, yet major mechanisms underlying survival remain poorly understood. We discover a posttranscriptional mechanism that integrates starvation response with GTP homeostasis to allow survival, enacted by the nucleotide (p)ppGpp, a key player i...

journal_title：Molecular cell

authors： Kriel A,Bittner AN,Kim SH,Liu K,Tehranchi AK,Zou WY,Rendon S,Chen R,Tu BP,Wang JD

更新日期：2012-10-26 00:00:00

abstract：:RNA:DNA hybrids in the genome are constantly being generated as a by-product of transcription; in this issue, two papers, from Helmrich et al. (2011) and Wahba et al. (2011), provide insight into how RNA:DNA hybrids lead to genetic instability. ...

journal_title：Molecular cell

authors： Potenski CJ,Klein HL

更新日期：2011-12-23 00:00:00

abstract：:Stress granules and P-bodies are cytosolic biomolecular condensates that dynamically form by the phase separation of RNAs and proteins. They participate in translational control and buffer the proteome. Upon stress, global translation halts and mRNAs bound to the translational machinery and other proteins coalesce to ...

journal_title：Molecular cell

authors： Youn JY,Dyakov BJA,Zhang J,Knight JDR,Vernon RM,Forman-Kay JD,Gingras AC

更新日期：2019-10-17 00:00:00

abstract：:Members of the ISWI family of chromatin remodeling factors exhibit ATP-dependent nucleosome sliding, loading, and spacing activities in vitro. However, it is unclear which of these activities are utilized by ISWI complexes to remodel chromatin in vivo. We therefore sought to identify the mechanisms of chromatin remode...

journal_title：Molecular cell

authors： Fazzio TG,Tsukiyama T

更新日期：2003-11-01 00:00:00

abstract：:BRCA1 and BRCA2 account for most cases of familial, early onset breast and/or ovarian cancer and encode products that each interact with hRAD51. Results presented here show that BRCA1 and BRCA2 coexist in a biochemical complex and colocalize in subnuclear foci in somatic cells and on the axial elements of developing s...

journal_title：Molecular cell

authors： Chen J,Silver DP,Walpita D,Cantor SB,Gazdar AF,Tomlinson G,Couch FJ,Weber BL,Ashley T,Livingston DM,Scully R

更新日期：1998-09-01 00:00:00

abstract：:Lysine acetylation of the tumor suppressor protein p53 in response to a wide variety of cellular stress signals is required for its activation as a transcription factor that regulates cell cycle arrest, senescence, or apoptosis. Here, we report that the conserved bromo-domain of the transcriptional coactivator CBP (CR...

journal_title：Molecular cell

authors： Mujtaba S,He Y,Zeng L,Yan S,Plotnikova O,Sachchidanand,Sanchez R,Zeleznik-Le NJ,Ronai Z,Zhou MM

更新日期：2004-01-30 00:00:00

abstract：:Dosage compensation in Drosophila is dependent on MSL proteins and involves hypertranscription of the male X chromosome, which ensures equal X-linked gene expression in both sexes. Here, we report the purification of enzymatically active MSL complexes from Drosophila embryos, Schneider cells, and human HeLa cells. We ...

journal_title：Molecular cell

authors： Mendjan S,Taipale M,Kind J,Holz H,Gebhardt P,Schelder M,Vermeulen M,Buscaino A,Duncan K,Mueller J,Wilm M,Stunnenberg HG,Saumweber H,Akhtar A

更新日期：2006-03-17 00:00:00

abstract：:Plant pattern recognition receptors (PRRs) perceive microbial and endogenous molecular patterns to activate immune signaling. The cytoplasmic kinase BIK1 acts downstream of multiple PRRs as a rate-limiting component, whose phosphorylation and accumulation are central to immune signal propagation. Previous work identif...

journal_title：Molecular cell

authors： Wang J,Grubb LE,Wang J,Liang X,Li L,Gao C,Ma M,Feng F,Li M,Li L,Zhang X,Yu F,Xie Q,Chen S,Zipfel C,Monaghan J,Zhou JM

更新日期：2018-02-01 00:00:00

abstract：:Trans-sialidases (TS) are GPI-anchored surface enzymes expressed in specific developmental stages of trypanosome parasites like Trypanosoma cruzi, the etiologic agent of Chagas disease, and T. brucei, the causative agent of sleeping sickness. TS catalyzes the transfer of sialic acid residues from host to parasite glyc...

journal_title：Molecular cell

authors： Buschiazzo A,Amaya MF,Cremona ML,Frasch AC,Alzari PM

更新日期：2002-10-01 00:00:00

abstract：:The identification of metazoan origins of DNA replication has so far been hampered by the lack of a suitable genetic screening and by the cumbersomeness of the currently available mapping procedures. Here we describe the construction of a library of nascent DNA, representative of all cellular origin sequences, and its...

journal_title：Molecular cell

authors： Todorovic V,Giadrossi S,Pelizon C,Mendoza-Maldonado R,Masai H,Giacca M

更新日期：2005-08-19 00:00:00

abstract：:Reactive oxygen species (ROS)-induced activation of Apoptosis signal-regulating kinase 1 (ASK1) plays crucial roles in oxidative stress-mediated cell death through the activation of the JNK and p38 MAPK pathways. However, the regulatory mechanism of ASK1 in the oxidative stress response remains to be elucidated. Here,...

journal_title：Molecular cell

authors： Sekine Y,Hatanaka R,Watanabe T,Sono N,Iemura S,Natsume T,Kuranaga E,Miura M,Takeda K,Ichijo H

更新日期：2012-12-14 00:00:00