A Finer Print Than TADs: PRC1-Mediated Domains.

abstract：:For many polarized cells, it is critical that the mitotic spindle becomes positioned relative to the polarity axis. This is especially important in yeast, where the site of cytokinesis is predetermined. The spindle position checkpoint (SPOC) therefore delays mitotic exit of cells with a mispositioned spindle. One comp...

journal_title：Molecular cell

authors： Pereira G,Schiebel E

更新日期：2005-07-22 00:00:00

abstract：:The identification of metazoan origins of DNA replication has so far been hampered by the lack of a suitable genetic screening and by the cumbersomeness of the currently available mapping procedures. Here we describe the construction of a library of nascent DNA, representative of all cellular origin sequences, and its...

journal_title：Molecular cell

authors： Todorovic V,Giadrossi S,Pelizon C,Mendoza-Maldonado R,Masai H,Giacca M

更新日期：2005-08-19 00:00:00

abstract：:p300/CBP and PCAF coactivators have acetyltransferase activities and regulate transcription, cell cycle progression, and differentiation. They are both required for MyoD activity and muscle differentiation. Nevertheless, their roles must be different since the acetyltransferase activity of PCAF but not of p300 is invo...

journal_title：Molecular cell

authors： Sartorelli V,Puri PL,Hamamori Y,Ogryzko V,Chung G,Nakatani Y,Wang JY,Kedes L

更新日期：1999-11-01 00:00:00

abstract：:Two publications in the current issue of Cell introduce novel methods for high-throughput single-cell transcriptomics by using droplet microfluidics and sophisticated barcoding schemes for transcriptional profiling of thousands of individual cells. ...

journal_title：Molecular cell

authors： Junker JP,van Oudenaarden A

更新日期：2015-05-21 00:00:00

abstract：:Polyubiquitination is a posttranslational modification where ubiquitin chains containing isopeptide bonds linking one of seven ubiquitin lysines with the C terminus of an adjoining ubiquitin are covalently attached to proteins. While functions of K48- and K63-linked polyubiquitin are understood, the role(s) of noncano...

journal_title：Molecular cell

authors： Matsumoto ML,Wickliffe KE,Dong KC,Yu C,Bosanac I,Bustos D,Phu L,Kirkpatrick DS,Hymowitz SG,Rape M,Kelley RF,Dixit VM

更新日期：2010-08-13 00:00:00

abstract：:The TATA binding protein (TBP) is required for the expression of nearly all genes and is highly regulated both positively and negatively. Here, we use DNA microarrays to explore the genome-wide interplay of several TBP-interacting inhibitors in the yeast Saccharomyces cerevisiae. Our findings suggest the following: Th...

journal_title：Molecular cell

authors： Chitikila C,Huisinga KL,Irvin JD,Basehoar AD,Pugh BF

更新日期：2002-10-01 00:00:00

abstract：:Trimethylation of histone H3 on lysine 4 (H3K4me3) localizes near the 5' region of genes and is tightly associated with active loci. Several proteins, such as CHD1, BPTF, JMJD2A, and the ING tumor suppressor family, directly recognize this lysine methyl mark. However, how H3K4me3 recognition participates in active tra...

journal_title：Molecular cell

authors： Sims RJ 3rd,Millhouse S,Chen CF,Lewis BA,Erdjument-Bromage H,Tempst P,Manley JL,Reinberg D

更新日期：2007-11-30 00:00:00

abstract：:Faithful propagation of functionally distinct chromatin states is crucial for maintaining cellular identity, and its breakdown can lead to diseases such as cancer. Whereas mechanisms that sustain repressed states have been intensely studied, regulatory circuits that protect active chromatin from inactivating signals a...

journal_title：Molecular cell

authors： Flury V,Georgescu PR,Iesmantavicius V,Shimada Y,Kuzdere T,Braun S,Bühler M

更新日期：2017-07-20 00:00:00

abstract：:Animal cells have a remarkable capacity to adopt durable and heritable gene expression programs or epigenetic states that define the physical properties and diversity of somatic cell types. The maintenance of epigenetic programs depends on poorly understood pathways that prevent gain or loss of inherited signals. In t...

journal_title：Molecular cell

authors： Ishidate T,Ozturk AR,Durning DJ,Sharma R,Shen EZ,Chen H,Seth M,Shirayama M,Mello CC

更新日期：2018-05-17 00:00:00

abstract：:Rho factor in bacteria terminates transcription by using energy from ATP hydrolysis to forcefully dissociate the transcripts from RNA polymerase. used data from presteady-state ATPase kinetics to support a rational mechanistic model for Rho's action on RNA. ...

journal_title：Molecular cell

authors： Richardson JP

更新日期：2006-06-23 00:00:00

abstract：:RING ubiquitin ligases (E3) recruit ubiquitin-conjugate enzymes (E2) charged with ubiquitin (Ub) to catalyze ubiquitination. Non-covalent Ub binding to the backside of certain E2s promotes processive polyUb formation, but the mechanism remains elusive. Here, we show that backside bound Ub (Ub(B)) enhances both RING-in...

journal_title：Molecular cell

authors： Buetow L,Gabrielsen M,Anthony NG,Dou H,Patel A,Aitkenhead H,Sibbet GJ,Smith BO,Huang DT

更新日期：2015-04-16 00:00:00

abstract：:Chaperone-mediated autophagy (CMA) is involved in cellular quality control, and in the response to stress, deterioration in CMA contributes to the aging phenotype and to various disorders. The paper by Bandyopadhyay et al. in this issue of Molecular Cell demonstrates the role of GTP binding by elongation factor 1 alph...

journal_title：Molecular cell

authors： Beau I,Codogno P

更新日期：2010-08-27 00:00:00

abstract：:Whipple et al., 2020 describe that, in neurons, a dense cluster of maternally expressed microRNAs post-transcriptionally downregulates several imprinted genes expressed from the paternal genome-an antagonistic mechanism that modulates neuronal functions and provides insights into the evolution of genomic imprinting. ...

journal_title：Molecular cell

authors： Ghousein A,Feil R

更新日期：2020-04-02 00:00:00

abstract：:Conjugation of ubiquitin (Ub) to a protein substrate targets the substrate for degradation or functional modification, which is tightly controlled by diverse mechanisms including phosphorylation of the substrate. An emerging mechanism involves regulation of the E3 Ub ligase, for example, the JNK-dependent phosphorylat...

journal_title：Molecular cell

authors： Yang C,Zhou W,Jeon MS,Demydenko D,Harada Y,Zhou H,Liu YC

更新日期：2006-01-06 00:00:00

abstract：:Linkage-specific polyubiquitin recognition is thought to make possible the diverse set of functional outcomes associated with ubiquitination. Thus far, mechanistic insight into this selectivity has been largely limited to single domains that preferentially bind to lysine 48-linked polyubiquitin (K48-polyUb) in isolati...

journal_title：Molecular cell

authors： Sims JJ,Cohen RE

更新日期：2009-03-27 00:00:00

abstract：:There are few tools available for dissecting and elucidating the functions of DNA satellites and other nongenic DNA. To address this, we have explored the experimental potential of DNA sequence-specific drugs containing pyrrole and imidazole amino acids (polyamides). Compounds were synthesized that target different Dr...

journal_title：Molecular cell

authors： Janssen S,Durussel T,Laemmli UK

更新日期：2000-11-01 00:00:00

abstract：:In eukaryotes, three-dimensional genome organization is critical for transcriptional regulation of gene expression. Long noncoding RNAs (lncRNAs) can modulate chromatin conformation of spatially related genomic locations within the nucleus. Here, we show that the lncRNA APOLO (AUXIN-REGULATED PROMOTER LOOP) recognizes...

journal_title：Molecular cell

authors： Ariel F,Lucero L,Christ A,Mammarella MF,Jegu T,Veluchamy A,Mariappan K,Latrasse D,Blein T,Liu C,Benhamed M,Crespi M

更新日期：2020-03-05 00:00:00

abstract：:The L. monocytogenes protein lnlB activates phosphoinositide 3-kinase and induces phagocytosis in several mammalian cell types. The 1.86 A resolution X-ray crystal structure of the leucine-rich repeat domain of lnlB that is both necessary and sufficient to induce phagocytosis is presented here. The structure supports ...

journal_title：Molecular cell

authors： Marino M,Braun L,Cossart P,Ghosh P

更新日期：1999-12-01 00:00:00

abstract：:Calreticulin and calnexin are homologous lectins that serve as molecular chaperones for glycoproteins in the endoplasmic reticulum of eukaryotic cells. Here we show that calreticulin depletion specifically accelerates the maturation of cellular and viral glycoproteins with a modest decrease in folding efficiency. Caln...

journal_title：Molecular cell

authors： Molinari M,Eriksson KK,Calanca V,Galli C,Cresswell P,Michalak M,Helenius A

更新日期：2004-01-16 00:00:00

abstract：:Three introns whose splicing is activated during meiosis in S. cerevisiae contain a Mer1p-dependent splicing enhancer. The enhancer can impose Mer1p-activated splicing upon the constitutively spliced actin intron provided the basal splicing efficiency of actin is first reduced. Of several nonessential splicing factors...

journal_title：Molecular cell

authors： Spingola M,Ares M Jr

更新日期：2000-08-01 00:00:00

abstract：:Replication forks frequently break and must be repaired by recombination. A reconstituted reaction now allows the factors that coordinate conversion from a recombination intermediate back to a replication fork to be defined. The PriA protein plays a key role in this control. ...

journal_title：Molecular cell

authors： Lovett ST

更新日期：2003-03-01 00:00:00

abstract：:How proteasomal ATPases stimulate the gate opening of 20S proteasomes is a longstanding question. In the September 7 issue of Molecular Cell, Smith et al. (2007) describe the conserved "HbYX" motif as the key to proteasome gate opening. ...

journal_title：Molecular cell

authors： Saeki Y,Tanaka K

更新日期：2007-09-21 00:00:00

abstract：:Autophagic turnover of intracellular constituents is critical for cellular housekeeping, nutrient recycling, and various aspects of growth and development in eukaryotes. Here we show that autophagy impacts the other major degradative route involving the ubiquitin-proteasome system by eliminating 26S proteasomes, a pro...

journal_title：Molecular cell

authors： Marshall RS,Li F,Gemperline DC,Book AJ,Vierstra RD

更新日期：2015-06-18 00:00:00

abstract：:Structural maintenance of chromosomes (SMC) complexes are essential for genome organization from bacteria to humans, but their mechanisms of action remain poorly understood. Here, we characterize human SMC complexes condensin I and II and unveil the architecture of the human condensin II complex, revealing two putativ...

journal_title：Molecular cell

authors： Kong M,Cutts EE,Pan D,Beuron F,Kaliyappan T,Xue C,Morris EP,Musacchio A,Vannini A,Greene EC

更新日期：2020-07-02 00:00:00

abstract：:Microtubule plus end binding proteins (+TIPs) localize to the dynamic plus ends of microtubules, where they stimulate microtubule growth and recruit signaling molecules. Three main +TIP classes have been identified (XMAP215, EB1, and CLIP-170), but whether they act upon microtubule plus ends through a similar mechanis...

journal_title：Molecular cell

authors： Slep KC,Vale RD

更新日期：2007-09-21 00:00:00

abstract：:A major unresolved question in messenger RNA translation is how ribosomal release factors terminate protein synthesis. Class 1 release factors decode stop codons and trigger hydrolysis of the bond between the nascent polypeptide and tRNA some 75 A away from the decoding site. While the gross features of the release fa...

journal_title：Molecular cell

authors： Trobro S,Aqvist J

更新日期：2007-09-07 00:00:00

abstract：:Polymerase gamma, which replicates and repairs mitochondrial DNA, requires the Pol gamma B subunit for processivity. We determined the crystal structure of mouse Pol gamma B, a core component of the mitochondrial replication machinery. Pol gamma B shows high similarity to glycyl-tRNA synthetase and dimerizes through a...

journal_title：Molecular cell

authors： Carrodeguas JA,Theis K,Bogenhagen DF,Kisker C

更新日期：2001-01-01 00:00:00

abstract：:Helicases unwind duplex DNA ahead of the polymerases at the replication fork. However, the identity of the eukaryotic replicative helicase has been controversial; in vivo studies implicate the ring-shaped heterohexameric Mcm2-7 complex, although only a specific subset of Mcm subunits (Mcm467) unwind DNA in vitro. To a...

journal_title：Molecular cell

authors： Bochman ML,Schwacha A

更新日期：2008-07-25 00:00:00

abstract：:Melanoma and other cancers harbor oncogenic mutations in the protein kinase B-Raf, which leads to constitutive activation and dysregulation of MAP kinase signaling. In order to elucidate molecular determinants responsible for B-Raf control of cancer phenotypes, we present a method for phosphoprotein profiling, using n...

journal_title：Molecular cell

authors： Old WM,Shabb JB,Houel S,Wang H,Couts KL,Yen CY,Litman ES,Croy CH,Meyer-Arendt K,Miranda JG,Brown RA,Witze ES,Schweppe RE,Resing KA,Ahn NG

更新日期：2009-04-10 00:00:00

abstract：:Necroptosis is an important form of lytic cell death triggered by injury and infection, but whether mixed lineage kinase domain-like (MLKL) is sufficient to execute this pathway is unknown. In a genetic selection for human cell mutants defective for MLKL-dependent necroptosis, we identified mutations in IPMK and ITPK1...

journal_title：Molecular cell

authors： Dovey CM,Diep J,Clarke BP,Hale AT,McNamara DE,Guo H,Brown NW Jr,Cao JY,Grace CR,Gough PJ,Bertin J,Dixon SJ,Fiedler D,Mocarski ES,Kaiser WJ,Moldoveanu T,York JD,Carette JE

更新日期：2018-06-07 00:00:00