A pre-ribosome with a tadpole-like structure functions in ATP-dependent maturation of 60S subunits.

abstract：:The anaphase-promoting complex (APC) is a ubiquitin ligase that promotes the degradation of cell-cycle regulators. Cdh1p is an APC coactivator that directly binds APC substrates. A genetic screen in budding yeast identified residues within Cdh1p critical for its function. Cdh1p proteins containing mutations within the...

journal_title：Molecular cell

authors： Burton JL,Tsakraklides V,Solomon MJ

更新日期：2005-05-27 00:00:00

abstract：:Viral initiators perform multiple functions in initiation of DNA replication including ori binding, melting, and unwinding, culminating in the formation of a double hexameric (DH) helicase. We have recapitulated the assembly of the papillomavirus E1 initiator DH helicase, providing the first description of how such a ...

journal_title：Molecular cell

authors： Schuck S,Stenlund A

更新日期：2005-11-11 00:00:00

abstract：:In this issue of Molecular Cell, Vonk et al. (2020) and Thiruvalluvan et al. (2020) identify key chaperones that confer resistance to protein aggregation in neural stem cells and become reduced upon differentiation. ...

journal_title：Molecular cell

authors： Molzahn CM,Mayor T

更新日期：2020-04-16 00:00:00

abstract：:Ataxia Telangiectasia Mutated (ATM) signaling is essential for the repair of a subset of DNA double-strand breaks (DSBs); however, its precise role is unclear. Here, we show that < or =25% of DSBs require ATM signaling for repair, and this percentage correlates with increased chromatin but not damage complexity. Impor...

journal_title：Molecular cell

authors： Goodarzi AA,Noon AT,Deckbar D,Ziv Y,Shiloh Y,Löbrich M,Jeggo PA

更新日期：2008-07-25 00:00:00

abstract：:Copy-number changes generate phenotypic variability in health and disease. Whether organisms protect against copy-number changes is largely unknown. Here, we show that Saccharomyces cerevisiae monitors the copy number of its ribosomal DNA (rDNA) and rapidly responds to copy-number loss with the clonal amplification of...

journal_title：Molecular cell

authors： Mansisidor A,Molinar T Jr,Srivastava P,Dartis DD,Pino Delgado A,Blitzblau HG,Klein H,Hochwagen A

更新日期：2018-11-01 00:00:00

abstract：:Dosage compensation ensures equal expression of X-linked genes in males and females. In Drosophila, equalization is achieved by hypertranscription of the male X chromosome. This process requires an RNA/protein containing dosage compensation complex (DCC). Here we use RNA interference of individual DCC components to de...

journal_title：Molecular cell

authors： Buscaino A,Köcher T,Kind JH,Holz H,Taipale M,Wagner K,Wilm M,Akhtar A

更新日期：2003-05-01 00:00:00

abstract：:The structure of an RNA polymerase II/general transcription factor TFIIF complex was determined by cryo-electron microscopy and single particle analysis. Density due to TFIIF was not concentrated in one area but rather was widely distributed across the surface of the polymerase. The largest subunit of TFIIF interacted...

journal_title：Molecular cell

authors： Chung WH,Craighead JL,Chang WH,Ezeokonkwo C,Bareket-Samish A,Kornberg RD,Asturias FJ

更新日期：2003-10-01 00:00:00

abstract：:The SMN complex assembles Sm cores on snRNAs, a key step in the biogenesis of snRNPs, the spliceosome's major components. Here, using SMN complex inhibitors identified by high-throughput screening and a ribo-proteomic strategy on formaldehyde crosslinked RNPs, we dissected this pathway in cells. We show that protein s...

journal_title：Molecular cell

authors： Yong J,Kasim M,Bachorik JL,Wan L,Dreyfuss G

更新日期：2010-05-28 00:00:00

abstract：:Linkage-specific polyubiquitin recognition is thought to make possible the diverse set of functional outcomes associated with ubiquitination. Thus far, mechanistic insight into this selectivity has been largely limited to single domains that preferentially bind to lysine 48-linked polyubiquitin (K48-polyUb) in isolati...

journal_title：Molecular cell

authors： Sims JJ,Cohen RE

更新日期：2009-03-27 00:00:00

abstract：:The mechanism by which (CTG)n expansion in the 3' UTR of the DMPK gene causes myotonic dystrophy (DM) is unknown. We identified four RNA splicing factors--hnRNP C, U2AF (U2 auxiliary factor), PTB (polypyrimidine tract binding protein), and PSF (PTB associated splicing factor)--that bind to two short regions 3' of the ...

journal_title：Molecular cell

authors： Tiscornia G,Mahadevan MS

更新日期：2000-06-01 00:00:00

abstract：:Signal-transduction cascades are usually studied on cell averages, masking variability between individual cells. To address this, we studied in individual cells the dynamic response of ERK2, a well-characterized MAPK signaling protein, which enters the nucleus upon stimulation. Using fluorescent tagging at the endogen...

journal_title：Molecular cell

authors： Cohen-Saidon C,Cohen AA,Sigal A,Liron Y,Alon U

更新日期：2009-12-11 00:00:00

abstract：:RNA-binding proteins coordinate the fates of multiple RNAs, but the principles underlying these global interactions remain poorly understood. We elucidated regulatory mechanisms of the RNA-binding protein HuR, by integrating data from diverse high-throughput targeting technologies, specifically PAR-CLIP, RIP-chip, and...

journal_title：Molecular cell

authors： Mukherjee N,Corcoran DL,Nusbaum JD,Reid DW,Georgiev S,Hafner M,Ascano M Jr,Tuschl T,Ohler U,Keene JD

更新日期：2011-08-05 00:00:00

abstract：:Trimethylation of histone H3 on lysine 4 (H3K4me3) localizes near the 5' region of genes and is tightly associated with active loci. Several proteins, such as CHD1, BPTF, JMJD2A, and the ING tumor suppressor family, directly recognize this lysine methyl mark. However, how H3K4me3 recognition participates in active tra...

journal_title：Molecular cell

authors： Sims RJ 3rd,Millhouse S,Chen CF,Lewis BA,Erdjument-Bromage H,Tempst P,Manley JL,Reinberg D

更新日期：2007-11-30 00:00:00

abstract：:The formation of silenced and condensed heterochromatin foci involves enrichment of heterochromatin protein 1 (HP1). HP1 can bridge chromatin segments and form liquid droplets, but the biophysical principles underlying heterochromatin compartmentalization in the cell nucleus are elusive. Here, we assess mechanisticall...

journal_title：Molecular cell

authors： Erdel F,Rademacher A,Vlijm R,Tünnermann J,Frank L,Weinmann R,Schweigert E,Yserentant K,Hummert J,Bauer C,Schumacher S,Al Alwash A,Normand C,Herten DP,Engelhardt J,Rippe K

更新日期：2020-04-16 00:00:00

abstract：:Ubiquitin-dependent proteolysis is an important mechanism that suppresses the beta-catenin transcription factor in cells without Wnt stimulation. A critical step in this regulatory pathway is to create a SCF(beta-TrCP) E3 ubiquitin ligase binding site for beta-catenin. Here we show that the SCF(beta-TrCP) binding site...

journal_title：Molecular cell

authors： Su Y,Fu C,Ishikawa S,Stella A,Kojima M,Shitoh K,Schreiber EM,Day BW,Liu B

更新日期：2008-12-05 00:00:00

abstract：:The genomes of mammalian neurons contain uniquely high levels of non-CG DNA methylation that can be bound by the Rett syndrome protein, MeCP2, to regulate gene expression. How patterns of non-CG methylation are established in neurons and the mechanism by which this methylation works with MeCP2 to control gene expressi...

journal_title：Molecular cell

authors： Clemens AW,Wu DY,Moore JR,Christian DL,Zhao G,Gabel HW

更新日期：2020-01-16 00:00:00

abstract：:Steroid hormones have rapid nongenomic effects on cell-signaling pathways, but the receptor mechanisms responsible for this are not understood. We have identified a specific polyproline motif in the amino-terminal domain of conventional progesterone receptor (PR) that mediates direct progestin-dependent interaction of...

journal_title：Molecular cell

authors： Boonyaratanakornkit V,Scott MP,Ribon V,Sherman L,Anderson SM,Maller JL,Miller WT,Edwards DP

更新日期：2001-08-01 00:00:00

abstract：:tRNAs reading four-codon families often have a modified uridine, cmo(5)U(34), at the wobble position of the anticodon. Here, we examine the effects on the decoding mechanism of a cmo(5)U modification in tRNA(1B)(Ala), anticodon C(36)G(35)cmo(5)U(34). tRNA(1B)(Ala) reads its cognate codons in a manner that is very simi...

journal_title：Molecular cell

authors： Kothe U,Rodnina MV

更新日期：2007-01-12 00:00:00

abstract：:In this issue of Molecular Cell, Tripathi and coworkers (Tripathi et al., 2010) decode some of the functions of a long noncoding RNA MALAT1. They provide evidence that MALAT1 regulates alternative splicing by controlling the activity of the SR protein family of splicing factors. ...

journal_title：Molecular cell

authors： Ankö ML,Neugebauer KM

更新日期：2010-09-24 00:00:00

abstract：:Physical and genetic studies verify that the DNA binding domain of protein gpNu1 (which initiates packaging of phage lambda DNA) is a winged helix-turn-helix (w HTH) and that gpNu1 dimers bind sites that are brought close through DNA bending. ...

journal_title：Molecular cell

authors： Campbell A

更新日期：2002-05-01 00:00:00

abstract：:In addition to its role as an electron transporter, mitochondrial nicotinamide adenine dinucleotide (NAD+) is an important co-factor for enzymatic reactions, including ADP-ribosylation. Although mitochondria harbor the most intra-cellular NAD+, mitochondrial ADP-ribosylation remains poorly understood. Here we provide ...

journal_title：Molecular cell

authors： Hopp AK,Teloni F,Bisceglie L,Gondrand C,Raith F,Nowak K,Muskalla L,Howald A,Pedrioli PGA,Johnsson K,Altmeyer M,Pedrioli DML,Hottiger MO

更新日期：2021-01-21 00:00:00

abstract：:CRISPR-Cas immunity requires integration of short, foreign DNA fragments into the host genome at the CRISPR locus, a site consisting of alternating repeat sequences and foreign-derived spacers. In most CRISPR systems, the proteins Cas1 and Cas2 form the integration complex and are both essential for DNA acquisition. M...

journal_title：Molecular cell

authors： Wright AV,Wang JY,Burstein D,Harrington LB,Paez-Espino D,Kyrpides NC,Iavarone AT,Banfield JF,Doudna JA

更新日期：2019-02-21 00:00:00

abstract：:Environmental cues provoke rapid transitions in gene expression to support growth and cellular plasticity through incompletely understood mechanisms. Lin28 RNA-binding proteins have evolutionarily conserved roles in post-transcriptional coordination of pro-growth gene expression, but signaling pathways allowing trophi...

journal_title：Molecular cell

authors： Amen AM,Ruiz-Garzon CR,Shi J,Subramanian M,Pham DL,Meffert MK

更新日期：2017-02-02 00:00:00

abstract：:Macroautophagy (autophagy) is a lysosome-dependent degradation process that has been implicated in age-associated diseases. Autophagy is involved in both cell survival and cell death, but little is known about the mechanisms that distinguish its use during these distinct cell fates. Here, we identify the microRNA miR-...

journal_title：Molecular cell

authors： Nelson C,Ambros V,Baehrecke EH

更新日期：2014-11-06 00:00:00

abstract：:PARP enzymes are increasingly taking on important roles beyond DNA repair. Huang et al. (2020b) report how the NAD+-dependent ADP-ribosylation of histone H2B by PARP-1 in complex with a metabolic enzyme suppresses the phosphorylation of an adjacent residue, impacting adipogenesis. ...

journal_title：Molecular cell

authors： Margulies CE,Ladurner AG

更新日期：2020-09-17 00:00:00

abstract：:We report the identification of histone PARylation factor 1 (HPF1; also known as C4orf27) as a regulator of ADP-ribosylation signaling in the DNA damage response. HPF1/C4orf27 forms a robust protein complex with PARP-1 in cells and is recruited to DNA lesions in a PARP-1-dependent manner, but independently of PARP-1 c...

journal_title：Molecular cell

authors： Gibbs-Seymour I,Fontana P,Rack JGM,Ahel I

更新日期：2016-05-05 00:00:00

abstract：:In this issue of Molecular Cell, Zhang et al. and Møller et al. independently report studies of the E. coli Hfq protein, revealing significant sequence similarities with human Sm proteins. Their findings suggest that Hfq, and the Sm and Sm-like (Lsm) proteins, may function in stabilizing interactions between RNA molec...

journal_title：Molecular cell

authors： Donahue WF,Jarrell KA

更新日期：2002-01-01 00:00:00

abstract：:The Polycomb repressive system is an essential chromatin-based regulator of gene expression. Despite being extensively studied, how the Polycomb system selects its target genes is poorly understood, and whether its histone-modifying activities are required for transcriptional repression remains controversial. Here, we...

journal_title：Molecular cell

authors： Blackledge NP,Fursova NA,Kelley JR,Huseyin MK,Feldmann A,Klose RJ

更新日期：2020-02-20 00:00:00

abstract：:Glycogen is the major mammalian glucose storage cache and is critical for energy homeostasis. Glycogen synthesis in neurons must be tightly controlled due to neuronal sensitivity to perturbations in glycogen metabolism. Lafora disease (LD) is a fatal, congenital, neurodegenerative epilepsy. Mutations in the gene encod...

journal_title：Molecular cell

authors： Raththagala M,Brewer MK,Parker MW,Sherwood AR,Wong BK,Hsu S,Bridges TM,Paasch BC,Hellman LM,Husodo S,Meekins DA,Taylor AO,Turner BD,Auger KD,Dukhande VV,Chakravarthy S,Sanz P,Woods VL Jr,Li S,Vander Kooi CW,Gentry

更新日期：2015-01-22 00:00:00

abstract：:In yeast Saccharomyces cerevisiae the G protein betagamma subunits (Ste4/Ste18) have long been known to transmit the signal required for mating. Here we demonstrate that GTPase-deficient mutants of Galpha (Gpa1) directly activate the mating response pathway. We also show that signaling by activated Gpa1 requires direc...

journal_title：Molecular cell

authors： Guo M,Aston C,Burchett SA,Dyke C,Fields S,Rajarao SJ,Uetz P,Wang Y,Young K,Dohlman HG

更新日期：2003-08-01 00:00:00