Labelling of rat vagal preganglionic neurones by carbocyanine dye DiI applied to the heart.

Abstract:

:We describe a method for applying the carbocyanine dye DiI to the rat heart that takes advantage of the dye's lipophilic properties and its ability to diffuse easily into tissues, and results in specific retrograde labelling of cardiac vagal preganglionic neurones in the medulla oblongata. Most of the labelled neurones were found bilaterally in the nucleus ambiguus (81%), with a few sparsely distributed in the dorsal motor vagal nucleus (6.5%), and in an intermediate area located between these two nuclei (12.5%). We contend that the method of applying DiI crystals to the surface of the heart is a more efficient, accurate and reproducible method of retrograde labelling than the injection of tracers into this very delicate tissue.

journal_name

Neuroreport

journal_title

Neuroreport

authors

Corbett EK,Batten TF,Kaye JC,Deuchars J,McWilliam PN

doi

10.1097/00001756-199904260-00004

subject

Has Abstract

pub_date

1999-04-26 00:00:00

pages

1177-81

issue

6

eissn

0959-4965

issn

1473-558X

journal_volume

10

pub_type

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