The T. cruzi trans-sialidase induces PC12 cell differentiation via MAPK/ERK pathway.


:The trans-sialidase (TS) of Trypanosoma cruzi induces survival and differentiation of neuronal and glial cells. This mechanism underlying survival is via phosphatidylinositol 3-kinase (PI3K) but how TS promotes neuronal differentiation remained to be determined. Here we show that TS-induced neurite outgrowth in PC12 cells is through sustained activation of the mitogen-activated protein kinase or ERK cascade and, therefore, by a signaling mechanism distinct from the one it uses to induce cell survival. Such differential activation of signal pathways in neuronal cells to effectuate diverse biological activities is analogous to the action of authentic neurotrophins and other growth factors, thereby reinforcing the novel concept of T. cruzi mimicry of host neurotrophic factor(s).






Chuenkova MV,Pereira MA




Has Abstract


2001-12-04 00:00:00












  • Antisense oligonucleotide to c-fos induces ipsilateral rotational behaviour to d-amphetamine.

    abstract::The immediate-early genes, especially c-fos, have been suggested to have an important role in neuronal plasticity and memory. However, it has been difficult to determine if in fact c-fos can participate in control of behaviour. Here we can report that direct unilateral infusion into the rat neostriatum of an antisense...


    pub_type: 杂志文章


    authors: Sommer W,Bjelke B,Ganten D,Fuxe K

    更新日期:1993-12-13 00:00:00

  • MK-801 does not enhance dopaminergic cell survival in embryonic nigral grafts.

    abstract::Two groups of hemiparkinsonian rats received grafts of embryonic ventral mesencephalon with or without the addition of the NMDA receptor antagonist (+)dizocilpine hydrogen maleate (MK-801). When added to the cell suspension, a 10 microM concentration of MK-801 did not enhance the survival of tyrosine hydroxylase posit...


    pub_type: 杂志文章


    authors: Schierle GS,Karlsson J,Brundin P

    更新日期:1998-05-11 00:00:00

  • Syllable-frequency effects in visual word recognition: evidence from ERPs.

    abstract::A lexical decision experiment was conducted while event related potentials (ERPs) were recorded. The word frequency and the first syllable frequency of each word were manipulated. Results showed that, while high frequency words produced less negative amplitudes in the N400 time window than low frequency words, the inv...


    pub_type: 杂志文章


    authors: Barber H,Vergara M,Carreiras M

    更新日期:2004-03-01 00:00:00

  • Apoptosis in metabolic myopathies.

    abstract::DNA fragmentation, the hallmark of apoptosis, has been recently investigated with contradictory results in several skeletal muscle disorders. Using in situ labeling of nuclear DNA fragmentation, we have tested the possibility that apoptosis might occur in muscles from patients with mitochondrial respiratory chain defe...


    pub_type: 杂志文章


    authors: Monici MC,Toscano A,Girlanda P,Aguennouz M,Musumeci O,Vita G

    更新日期:1998-07-13 00:00:00

  • Olfactory bulb ensheathing cells enhance peripheral nerve regeneration.

    abstract::Sciatic nerve resection leaving a 15 mm gap could not be repaired by bridging the stumps with a silicone tube prefilled with a laminin gel. However, when purified olfactory ensheathing cells (EC) were added to the gel filling the tube, successful axonal regeneration was observed in 50% of rats. With 12 mm gaps, regene...


    pub_type: 杂志文章


    authors: Verdú E,Navarro X,Gudiño-Cabrera G,Rodríguez FJ,Ceballos D,Valero A,Nieto-Sampedro M

    更新日期:1999-04-06 00:00:00

  • Postsynaptic 5-HT1A receptors control 5-HT release in the rat medial prefrontal cortex.

    abstract::5-HTt1A receptor agonists reduce the neuronal release of 5-hydroxytryptamine (5-HT) by activation of raphe 5-HT1A autoreceptors. Using in vivo microdialysis in unanesthetized rats, we show that the local application of the selective 5-HT1A receptor agonist 8-OH-DPAT decreased the 5-HT output to approximately 50% of co...


    pub_type: 杂志文章


    authors: Casanovas JM,Hervás I,Artigas F

    更新日期:1999-05-14 00:00:00

  • Spontaneous axonal regeneration after optic nerve injury in adult rat.

    abstract::Optic nerves of adult rats were crushed 2 mm behind the eye to examine the ability of retinal ganglion cells (RGCs) to regenerate their axons. Some animals were treated with the immunophilin ligands FK 506 or GPI 1046 for up to 4 weeks. After 10 days to 16 months, regenerating RGC axons were visualized using anterogra...


    pub_type: 杂志文章


    authors: Campbell G,Holt JK,Shotton HR,Anderson PN,Bavetta S,Lieberman AR

    更新日期:1999-12-16 00:00:00

  • Open field behaviour in rats: role of metabotropic glutamate receptors.

    abstract::Previous studies have shown that spatial memory retention is affected by the metabotropic glutamate receptor (mGluR) influencing agents (R,S)-alpha-methyl-4-carboxyphenylglycine (MCPG) and trans-azetidine-2,4-dicarboxylic acid (tADA). In the present investigation we examined whether the mGluR antagonist MCPG (20 mM an...


    pub_type: 杂志文章


    authors: Wetzel W,Wagner T,Reymann KG,Riedel G

    更新日期:1995-11-27 00:00:00

  • The effects of patterned electrical stimulation combined with voluntary contraction on spinal reciprocal inhibition in healthy individuals.

    abstract::The aim of this study was to examine the effects of voluntary contraction (VC) on the modulation of reciprocal inhibition induced by patterned electrical stimulation (PES) in healthy individuals. Twelve healthy volunteers participated in this study. PES was applied to the common peroneal nerve with a train of 10 pulse...


    pub_type: 杂志文章


    authors: Takahashi Y,Fujiwara T,Yamaguchi T,Kawakami M,Mizuno K,Liu M

    更新日期:2017-05-24 00:00:00

  • Nitric oxide regulates NMDA-induced dopamine release in rat striatum.

    abstract::We investigated the effect of nitric oxide (NO) on N-methyl-D-aspartate (NMDA)-induced changes in levels of dopamine (DA) and its metabolite in the striatum using in vivo microdialysis. Local administration of 1mM NMDA into the striatum significantly augmented DA release in the striatum. Simultaneous administration of...


    pub_type: 杂志文章


    authors: Shibata M,Araki N,Ohta K,Hamada J,Shimazu K,Fukuuchi Y

    更新日期:1996-01-31 00:00:00

  • Neural correlates of encoding processes predicting subsequent cued recall and source memory.

    abstract::In this experiment, event-related potentials were used to examine whether the neural correlates of encoding processes predicting subsequent successful recall differed from those predicting successful source memory retrieval. During encoding, participants studied lists of words and were instructed to memorize each word...


    pub_type: 杂志文章


    authors: Angel L,Isingrini M,Bouazzaoui B,Fay S

    更新日期:2013-03-06 00:00:00

  • Temporal prediction restores the evaluative processing of delayed action feedback: an electrophysiological study.

    abstract::The evaluative processing of the valence of action feedback is reflected by an event-related brain potential component called feedback-related negativity (FRN) or reward positivity (RewP). Recent studies have shown that FRN/RewP is markedly reduced when the action-feedback interval is long (e.g. 6000 ms), indicating t...


    pub_type: 杂志文章


    authors: Kimura K,Kimura M

    更新日期:2016-09-28 00:00:00

  • Localization of the somatostatin sst2(a) receptor in human cerebral cortex, hippocampus and cerebellum.

    abstract::The distribution and cellular localization of the somatostatin sst2(a) receptor was investigated in selected human brain areas using an anti-peptide antibody raised against a carboxy-terminal portion of the receptor protein. The sst2(a) receptor was found to be present on neurones and processes in the deep layers of t...


    pub_type: 杂志文章


    authors: Schindler M,Holloway S,Humphrey PP,Waldvogel H,Faull RL,Berger W,Emson PC

    更新日期:1998-02-16 00:00:00

  • Hypoxia-induced decrease of brain acetylcholine release detected by microdialysis.

    abstract::The in-vivo extracellular release of acetylcholine (ACh) from the striatum of rats was measured by intracerebral microdialysis. The ACh levels were first estimated under normoxic conditions, after which the rats were exposed to either 10% or 5% oxygen for 1 h. The average ACh content was 2.9 pmol/20 min. During hypoxi...


    pub_type: 杂志文章


    authors: Chleide E,Ishikawa K

    更新日期:1990-11-01 00:00:00

  • Diversity of aquaporin mRNA expressed by rat and human retinas.

    abstract::Muller glial cells of the sensory retina mediate K+ and water fluxes that are facilitated by aquaporin-4 (AQP4) water channels and by Kir4.1-K+ channels. However, it is not known which subtypes of aquaporins are expressed in the mammalian retina. Using RT-PCR, we found that both human and rat retinas express mRNA for ...


    pub_type: 杂志文章


    authors: Tenckhoff S,Hollborn M,Kohen L,Wolf S,Wiedemann P,Bringmann A

    更新日期:2005-01-19 00:00:00

  • 'Prepulse inhibition' facilitates a liberal response bias in an auditory discrimination task.

    abstract::This study examined 'prepulse inhibition' in the context of an auditory two-tone discrimination task performed by 15 healthy subjects. In order to distinguish between masking or excitatory information processes, weak acoustic pulses immediately preceded or succeeded a target or non-target tone in the discrimination. B...


    pub_type: 临床试验,杂志文章


    authors: Schall U,Ward PB

    更新日期:1996-01-31 00:00:00

  • Nuclear translocation of GAPDH-GFP fusion protein during apoptosis.

    abstract::Increased expression and nuclear accumulation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) are early, critical events in several forms of apoptosis. In order to investigate the subcellular trafficking of GAPDH in vivo, the localization of a GAPDH-green fluorescent protein (GFP) fusion was studied in PC12, HEK 2...


    pub_type: 杂志文章


    authors: Shashidharan P,Chalmers-Redman RM,Carlile GW,Rodic V,Gurvich N,Yuen T,Tatton WG,Sealfon SC

    更新日期:1999-04-06 00:00:00

  • The role of miR-122-5p in negatively regulating T-box brain 1 expression on the differentiation of mouse bone mesenchymal stem cells.

    abstract::To achieve neuronal differentiation of mouse bone mesenchymal stem cells (bMSCs) into neuron-like cells and explore the role of miR-122-5p that may regulate T-box brain 1 (Tbr1) expression during the induction. BMSCs were cultured and induced with butylated hydroxyanisole, retinoic acid (RA), basic fibroblast growth f...


    pub_type: 杂志文章


    authors: Yang Y,Shen Z,Sun W,Gao S,Li Y,Guo Y

    更新日期:2017-05-03 00:00:00

  • Innervation of stem cell-derived neurons into auditory epithelia of mice.

    abstract::This study examined the potential of embryonic stem cell-derived neurons as transplants for cell therapy for the primary loss of spiral ganglion neurons. To assess the ability of embryonic stem cell-derived neurons for innervation into auditory epithelia, they were cocultured with auditory epithelium explants of mice ...


    pub_type: 杂志文章


    authors: Matsumoto M,Nakagawa T,Higashi T,Kim TS,Kojima K,Kita T,Sakamoto T,Ito J

    更新日期:2005-05-31 00:00:00

  • Cue-induced renewal of heroin place preference: involvement of the basolateral amygdala.

    abstract::It is well established that re-exposure to a context paired with the effects of drugs of abuse can renew extinguished drug seeking behavior. A context, however, typically includes several stimuli, which may differ in their ability to control drug-oriented behaviors. Hence, the primary objective of this study was to as...


    pub_type: 杂志文章


    authors: Cummins E,Boughner E,Leri F

    更新日期:2014-03-26 00:00:00

  • An event-related potential investigation of spatial attention orientation in children trained with mental abacus calculation.

    abstract::The objective of this study was to investigate the effects of long-term mental abacus calculation training (MACT) on children's spatial attention orientation. Fifteen children with intensive MACT (MACT group) and 15 children without MACT (non-MACT group) were selected. The two groups of children were matched in age, s...


    pub_type: 杂志文章


    authors: Liu X,Sun Y

    更新日期:2017-01-01 00:00:00

  • Structural and functional connectivity correlates with motor impairment in chronic supratentorial stroke: a multimodal magnetic resonance imaging study.

    abstract::The aim of this study was to identify differences in structural and functional brain connectivity between poststroke mild and severe motor impairment. Twenty-four chronic stroke patients who underwent resting-state functional MRI and diffusion tensor image were retrospectively included. All patients were classified in...


    pub_type: 杂志文章


    authors: Lee JH,Kyeong S,Kang H,Kim DH

    更新日期:2019-05-01 00:00:00

  • Magnesium deficiency induces an hyperalgesia reversed by the NMDA receptor antagonist MK801.

    abstract::The aim of this study was to determine the changes of the nociceptive thresholds in response to an acute mechanical stimulus (paw pressure) in magnesium (Mg)-deficient rats, and the involvement of the NMDA receptor in these changes. Changes in vocalization thresholds was determined after 7 days of feeding with a Mg-de...


    pub_type: 杂志文章


    authors: Dubray C,Alloui A,Bardin L,Rock E,Mazur A,Rayssiguier Y,Eschalier A,Lavarenne J

    更新日期:1997-04-14 00:00:00

  • NMDA blockade attenuates caspase-3 activation and DNA fragmentation after neonatal hypoxia-ischemia.

    abstract::The aim was to study the effects of an NMDA receptor antagonist on caspase-3 activation and DNA fragmentation after hypoxia-ischemia (HI) in 7-day-old rats. Animals were treated with vehicle or MK-801 (0.5 mg/kg) directly after HI and sacrificed 8, 24 or 72h later. MK-801 reduced injury (by 53%), cells positive for ac...


    pub_type: 杂志文章


    authors: Puka-Sundvall M,Hallin U,Zhu C,Wang X,Karlsson JO,Blomgren K,Hagberg H

    更新日期:2000-09-11 00:00:00

  • NMDA receptor subunit and CaMKII changes in rat hippocampus by congenital HCMV infection: a mechanism for learning and memory impairment.

    abstract::The aim of this study was to investigate the effects of congenital human cytomegalovirus infection on the expression levels of N-methyl-D-aspartate receptors (NRs) and Ca/calmodulin-dependent protein kinase II (CaMKII) in the hippocampal neurons of neonatal Sprague-Dawley (SD) rats. Pregnant SD rats were divided into ...


    pub_type: 杂志文章


    authors: Wu,Yang L,Bu X,Tang J,Fan X

    更新日期:2017-03-22 00:00:00

  • Four facets of a single brain: behaviour, cerebral blood flow/metabolism, neuronal activity and neurotransmitter dynamics.

    abstract::Is functional neuroimaging a royal way to understand brain function or is it a new phrenology without an exact understanding what we measure? After two decades of imaging revolution, more and more authors ask this question. Brain functions are multidimensional, which can be approached from the point of (1) behavioural...


    pub_type: 杂志文章,评审


    authors: Kéri S,Gulyás B

    更新日期:2003-06-11 00:00:00

  • Opioid receptors in the human cerebellum: evidence from [11C]diprenorphine PET, mRNA expression and autoradiography.

    abstract::Little is known regarding opioid receptors in the human cerebellum. The present [11C]diprenorphine PET study investigated opioid receptor binding in the human cerebellum in vivo, and showed a differential binding level in cerebellar cortex, vermis and dentate nuclei. The additional study in vitro of opioid receptors i...


    pub_type: 杂志文章


    authors: Schadrack J,Willoch F,Platzer S,Bartenstein P,Mahal B,Dworzak D,Wester HJ,Zieglgänsberger W,Tölle TR

    更新日期:1999-02-25 00:00:00

  • Repetitive transcranial magnetic stimulation over the right dorsolateral prefrontal cortex affects strategic decision-making.

    abstract::Although decision-making is typically seen as a rational process, emotions play a role in tasks that include unfairness. Recently, activation in the right dorsolateral prefrontal cortex during offers experienced as unfair in the Ultimatum Game was suggested to subserve goal maintenance in this task. This is restricted...


    pub_type: 杂志文章


    authors: van 't Wout M,Kahn RS,Sanfey AG,Aleman A

    更新日期:2005-11-07 00:00:00

  • Reproductive senescence predicts cognitive decline in aged female monkeys.

    abstract::The present investigation provide evidences from a non-human primate model that naturally occurring menopause predicts a prominent signature of age-related cognitive decline. Young and aged rhesus monkeys were tested on a delayed response (DR) task known to the sensitive to aging, and reproductive status was evaluated...


    pub_type: 杂志文章


    authors: Roberts JA,Gilardi KV,Lasley B,Rapp PR

    更新日期:1997-05-27 00:00:00

  • Transfection of young guinea pig vestibular cells in vitro with an adenovirus vector.

    abstract::This study shows distributions of lacZ-positive cells in the vestibular labyrinthine explants of young guinea pigs with mature ears. When adenovirus lacZ vectors were administered to the vestibular labyrinth following removal of the otoconial membrane, lacZ-positive cells were observed in the mesothelial cells in the ...


    pub_type: 杂志文章


    authors: Suzuki M,Yamasoba T,Kondo K,Kaga K

    更新日期:2001-12-21 00:00:00