Abstract:
:Selective recruitment of periodontal ligament cells to a previously exposed root surface is believed to enhance periodontal regeneration. It has been hypothesized that competition from gingival fibroblasts may reduce the potential of periodontal regeneration. We compared the migratory responses of PDL cells and gingival fibroblasts to a variety of biologicals. Parallel experiments designed to examine the directed migration responses of both periodontal ligament cells (PDL cells) and gingival fibroblasts (GF) isolated from the same donors were conducted using Platelet Derived Growth Factor (PDGF), Insulin Like Growth Factor-I, -II (IGF-I, -II), Epidermal Growth Factor (EGF), Transforming Growth Factor-beta (TGF-beta), and the chemotactic factor derived from the conditioned culture media of PDL cells (termed PDL-CTX) as attractants. Both PDL cells and GF exhibited dose-dependent migratory responses when challenged with PDGF, IGF-I, IGF-II, EGF, and TGF-beta. However, when these cells were challenged with PDL-CTX, only PDL cells migrated in a specific dose-dependent manner, while GF were refractive to PDL-CTX stimulation. Additionally, concentrated conditioned culture media from cultures of gingival fibroblasts did not stimulate PDL cell migratory responses. In other experiments, antibody directed against PDGF, FGF, TGF-beta, IGF-I, IGF-II, NGF, and EGF did not inhibit the PDL-CTX-elicited response in PDL cells. Previous studies have suggested that success of periodontal therapy depends on the specific attachment, migration, and proliferation of selected periodontal ligament cells. The data presented in this manuscript suggest that both PDL cells and gingival fibroblasts respond to a multitude of growth factors. PDL-CTX was found to be PDL-cell-specific for directed migration. Thus, we conclude that any biological therapeutic regime for periodontal regeneration should include PDL-cell-specific agents.
journal_name
J Dent Resjournal_title
Journal of dental researchauthors
Nishimura F,Terranova VPdoi
10.1177/00220345960750041401subject
Has Abstractpub_date
1996-04-01 00:00:00pages
986-92issue
4eissn
0022-0345issn
1544-0591journal_volume
75pub_type
杂志文章abstract::The development of predictive and rapid methods for the assessment of the anticaries activity of topical fluorides has been a long-standing objective of caries researchers. These methods can provide useful benefits in a number of applications, ranging from the identification of novel agents to progress into clinical t...
journal_title:Journal of dental research
pub_type: 杂志文章,评审
doi:10.1177/002203459207100S19
更新日期:1992-04-01 00:00:00
abstract::A number of studies have been made investigating the properties of dental prosthodontic soft polymer materials. In such materials, which are used as short-term denture soft liners, the polymer component is complexed with one or more plasticizers. This lowers the glass transition temperature, Tg, of the polymer by allo...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345910700050201
更新日期:1991-05-01 00:00:00
abstract::The complexity of human oral functional movements has not been studied in detail quantitatively, and only recently have studies begun to evaluate whether such movements contain sex-specific characteristics. Therefore, the purposes of this study were: (1) to quantify in detail the jaw movements and associated masticato...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345970760031301
更新日期:1997-03-01 00:00:00
abstract::The hypothesis that periodontal disease is associated with pre-term low birthweight was tested in a prospective follow-up study of rural prima-gravida women (N = 227) who were free of tobacco, alcohol, and drug use. Women with 3rd trimester mean probing pocket depths, plaque, and bleeding scores that were greater than...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910508400313
更新日期:2005-03-01 00:00:00
abstract::Angiogenesis and biomineral substrates play major roles in bone development and regeneration. We hypothesized that macroporous scaffolds of biomineralized 85:15 poly(lactide-co-glycolide), which locally release vascular endothelial growth factor-165 (VEGF), would direct simultaneous regeneration of bone and vascular t...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910408300304
更新日期:2004-03-01 00:00:00
abstract::Recent evidence implicates endothelin in nociception, but it is unclear how endothelin activates trigeminal ganglion (TRG) neurons. In the present study, we investigated the expression of the endothelin receptors ETA and ETB and endothelin-induced responses in rat TRG neurons. Double-immunofluorescence studies demonst...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034513478428
更新日期:2013-04-01 00:00:00
abstract::Chondroitin sulfate is up-regulated in granulation tissue during wound healing. To investigate the role of chondroitin sulfate in the wound-healing process after surgical repair of cleft palate, we isolated and cultured rabbit palatal fibroblasts. Treatment with chondroitin-6-sulfate resulted in a dose-dependent incre...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910408301111
更新日期:2004-11-01 00:00:00
abstract::Chemical bonding between dental porcelains and alloys results from interdiffusion of porcelain and metal ions. An interfacial diffusion zone is created which, most likely, has properties different from those of bulk materials. The changed interface might affect experimental measurements of thermo-mechanical strain. To...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345830620051701
更新日期:1983-05-01 00:00:00
abstract::Thermal diffusivity, a property related to the thermal insulative efficiency of a material, was measured in nine glass-ionomer cements and compared with results from a silicate and a polycarboxylate cement. Each cement was mixed at various powder-liquid ratios (P/L) and moulded into a rectangular prism of approximate ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345870660051001
更新日期:1987-05-01 00:00:00
abstract::In previous studies, human dental pulp stem cells (hDPSCs) were mainly isolated from adults. In this present study, we characterized hDPSCs isolated from an earlier developmental stage to evaluate the potential usage of these cells for tissue-regenerative therapy. hDPSCs isolated at the crown-completed stage showed a ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910808700716
更新日期:2008-07-01 00:00:00
abstract:UNLABELLED:The aim of this observational study was to investigate the features of the chewing activity and the variability of the human chewing pace, as assessed in the natural environment. It was hypothesized that the chewing pace is relatively constant within individuals across different days but is variable across i...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034511416669
更新日期:2011-10-01 00:00:00
abstract::Lysozyme concentration was quantitated immunochemically in parotid and submandibular-sublingual saliva of 46 caries-resistant and 17 caries-susceptible adults. There was essentially no difference between the two groups. The concentration of lysozyme was three times higher in the submandibular-sublingual than in the pa...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345830620050701
更新日期:1983-05-01 00:00:00
abstract::In an attempt to determine the degree of co-activation present in selected cervical muscles during clenching, we instructed 12 male subjects to produce four brief maximum voluntary contraction (MVC) efforts (clenching) in a position of maximum intercuspation. Surface EMG activity was recorded bilaterally from the mass...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345930720110701
更新日期:1993-11-01 00:00:00
abstract::Fibroblast growth factor (FGF) signaling has been implicated in the regulation of osteogenesis in both intramembranous and endochondral ossifications. In the developing palate, the anterior bony palate forms by direct differentiation of cranial neural crest (CNC)-derived mesenchymal cells, but the signals that regulat...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034517750141
更新日期:2018-05-01 00:00:00
abstract::Teeth undergo postnatal organogenesis relatively late in life and only complete full maturation a few years after the crown first erupts in the oral cavity. At this stage, development can be arrested if the tooth organ is damaged by either trauma or caries. Regenerative endodontic procedures (REPs) are a treatment alt...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034517693606
更新日期:2017-06-01 00:00:00
abstract::The effect of inflammation on the distribution of chondroitin sulfate and dermatan sulfate proteoglycans was assessed after normal and inflamed human gingivae were stained with monoclonal antibodies against these extracellular matrix macromolecules. The tissues were obtained following periodontal surgery and reacted w...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345920710090801
更新日期:1992-09-01 00:00:00
abstract::The aim of the present in vitro experiments was to determine whether the protection of enamel by topically applied fluoride against demineralization by bacterial acids would depend on the fluoride sensitivity of the bacteria. Glucose-agarose gel suspensions of fluoride-sensitive and fluoride-resistant mutans streptoco...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345930720080401
更新日期:1993-08-01 00:00:00
abstract::The major phosphoprotein in dentin is the aspartic acid and serine-rich protein called dentin phosphophoryn (DPP). DPP appears to be synthesized as a part of a larger compound protein, dentin sialophosphoprotein (DSPP). DSPP has never been isolated or detected in dentin extracts. It is now evident that DSPP is a chime...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034513516631
更新日期:2014-02-01 00:00:00
abstract::The cariostatic benefit from water fluoridation is indisputable, but the knowledge of possible adverse effects on bone and fracture risk due to fluoride exposure is ambiguous. The association between long-term (chronic) drinking water fluoride exposure and hip fracture (ICD-7-9: '820' and ICD-10: 'S72.0-S72.2') was as...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034513506443
更新日期:2013-11-01 00:00:00
abstract::Stem cells are remarkable, and stem cell-based tissue engineering is an emerging field of biomedical science aiming to restore damaged tissue or organs. In dentistry and reconstructive facial surgery, it is of great interest to restore lost teeth or craniofacial bone defects using stem cell-mediated therapy. In the cr...
journal_title:Journal of dental research
pub_type: 杂志文章,评审
doi:10.1177/0022034515603972
更新日期:2015-11-01 00:00:00
abstract::An in vitro model has been developed for measurement of initial attachment of 3H-labeled human gingival fibroblasts (HGF) and periodontal ligament cells (HPDL cells) to sieved dentin powder. Reproducible attachment was obtained and was closely related to the number of plated cells, the amount of dentin powder, the tem...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345930720030901
更新日期:1993-03-01 00:00:00
abstract::Greater fluoride concentrations were obtained in enamel treated with phosphate/fluoride solutions than in that treated with TiF4 at similar pH's (1.0) and F concentrations (0.6M F and 1.6M F). However, fluoride solution without added phosphate at pH 1.0 and 1.6M F concentration produced lower fluoride concentrations i...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345790580021001
更新日期:1979-02-01 00:00:00
abstract::With clinical oral examinations not always possible in health surveys, researchers may instead be invited to add questions to a wider health survey. In such situations, an item is needed which adequately represents both clinical and self-reported oral health. This study investigated the clinical validity of Locker's g...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034512460676
更新日期:2012-11-01 00:00:00
abstract::Medication-related osteonecrosis of the jaw (MRONJ), although initially believed to be exclusively associated with bisphosphonates, has been implicated in recent reports with additional drugs, especially the bone antiresorptive denosumab. The pathophysiology has not been fully elucidated, and no causal association bet...
journal_title:Journal of dental research
pub_type: 杂志文章,评审
doi:10.1177/0022034515572021
更新日期:2015-04-01 00:00:00
abstract::Matrix metalloproteinases (MMPs) produced by resident and inflammatory cells in response to periodontopathogens play a major role in periodontal tissue destruction. Our aim was to investigate the effects of A-type cranberry proanthocyanidins (AC-PACs) on: (i) the production of various MMPs by human monocyte-derived ma...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034509339487
更新日期:2009-07-01 00:00:00
abstract::The cartilaginous nasal septa of 28 albino rats were marked with pairs of wire implants. Measurements obtained from cephalometric radiographs taken at 30 and 60 days revealed that the implants invariably separatd and did so at a rate that was highly correlated with the growth of the rest of the snout. ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345800590081601
更新日期:1980-08-01 00:00:00
abstract::The monomer triethyleneglycoldimethacrylate (TEGDMA) is used as a diluent in many resin-based dental materials. It was previously shown in vitro that TEGDMA was released into the adjacent biophase from such materials during the first days after placement. In this study, the uptake, distribution, and excretion of 14C-T...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345010800050501
更新日期:2001-05-01 00:00:00
abstract::Although dental amalgam is used frequently under artificial crowns for restoration of severely damaged teeth, there is little information available on the bond between luting cements and this alloy. This study was designed for determination of the strength of the bond between a dental amalgam alloy and three crown-lut...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345890680111501
更新日期:1989-11-01 00:00:00
abstract::The field of dental materials has undergone more of a revolution than an evolution over the past 100 y. The development of new products, especially in the past half century, has occurred at a staggering pace, and their introduction to the market has been equally impressive. The movement has mostly come in the area of ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034518822808
更新日期:2019-03-01 00:00:00
abstract::The firing pattern of the motor units (MUs) in jaw-elevator muscles was studied within a wide range of isometric contraction levels by means of changes in the frequency and broadness of the primary peak in the low-frequency (5-40 Hz) power spectral density function of the surface EMG. EMG was recorded from both masset...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345940730051501
更新日期:1994-05-01 00:00:00