Abstract：
:Teeth undergo postnatal organogenesis relatively late in life and only complete full maturation a few years after the crown first erupts in the oral cavity. At this stage, development can be arrested if the tooth organ is damaged by either trauma or caries. Regenerative endodontic procedures (REPs) are a treatment alternative to conventional root canal treatment for immature teeth. These procedures rely on the transfer of apically positioned stem cells, including stem cells of the apical papilla (SCAP), into the root canal system. Although clinical success has been reported for these procedures, the predictability of expected outcomes and the organization of the newly formed tissues are affected by the lack of an available suitable scaffold that mimics the complexity of the dental pulp extracellular matrix (ECM). In this study, we evaluated 3 methods of decellularization of human dental pulp to be used as a potential autograft scaffold. Tooth slices of human healthy extracted third molars were decellularized by 3 different methods. One of the methods generated the maximum observed decellularization with minimal impact on the ECM composition and organization. Furthermore, recellularization of the scaffold supported the proliferation of SCAP throughout the scaffold with differentiation into odontoblast-like cells near the dentinal walls. Thus, this study reports that human dental pulp from healthy extracted teeth can be successfully decellularized, and the resulting scaffold supports the proliferation and differentiation of SCAP. The future application of this form of an autograft in REPs can fulfill a yet unmet need for a suitable scaffold, potentially improving clinical outcomes and ultimately promoting the survival and function of teeth with otherwise poor prognosis.
journal_name
J Dent Resjournal_title
Journal of dental researchauthors
Song JS,Takimoto K,Jeon M,Vadakekalam J,Ruparel NB,Diogenes Adoi
10.1177/0022034517693606subject
Has Abstractpub_date
2017-06-01 00:00:00pages
640-646issue
6eissn
0022-0345issn
1544-0591journal_volume
96pub_type
杂志文章abstract：:Periodontal ligament (PDL) cells play an important role in maintaining the homeostasis of periodontal tissues. However, it is not known how PDL cells contribute to osteoclastogenesis. In this study, we examined the consequences of cell-to-cell interactions between peripheral blood mononuclear cells (PBMCs) and PDL cel...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345010800030801
更新日期：2001-03-01 00:00:00
abstract：:Periodontal inflammation and alveolar bone remodeling during orthodontic tooth movement are considered regional reactions. However, how systemic immune responses are involved in this regional reaction remains unclear. In this study, we explored the systemic effects of orthodontic force by focusing on the mononuclear p...
journal_title：Journal of dental research
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doi：10.1177/0022034515592868
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abstract：:Composite resin restorations in cavities are generally associated with the development of a contraction gap during the first minutes after insertion. Enamel-Bond-Concise composite restoration were placed on both approximal sides of extracted pre-molars. The cervical walls of all cavities extended beyond the enamel-cem...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345840630051501
更新日期：1984-05-01 00:00:00
abstract：:Human tooth wear occurs so slowly that traditionally it has needed months or years to be measurable. This study showed that microscopic changes in wear patterns on human teeth could be detected in a matter of days and could be used as indicators of rates of wear. Thus, daily or weekly changes in rates of wear can be d...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345910700030901
更新日期：1991-03-01 00:00:00
abstract：:This paper aims to present a simple overview of potential new diagnostic methods for dental caries. There are several novel methods of caries detection (with potential application to diagnosis) which have been proposed in the last few years, in addition to those that are gaining some commercial exposure and clinical a...
journal_title：Journal of dental research
pub_type： 杂志文章,评审
doi：10.1177/154405910408301s18
更新日期：2004-01-01 00:00:00
abstract：:The range of substrates transported by the sugar-binding protein-dependent msm (multiple sugar metabolism) system of S. mutans was investigated. By determining the ability of unlabeled sugar to compete with radiolabeled melibiose transport, we have demonstrated that the transported sugars included a number of carbohyd...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345930720100701
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abstract：:The spatial relationship of the mandibular condyle to the temporal component of the TMJ is determined quantitatively from lateral tomograms using a new method of measuring specific areas of the joint space. Condyle position along the posteroanterior axis of the joint is expressed as a ratio of the posterior joint spac...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345810600010701
更新日期：1981-01-01 00:00:00
abstract：:Seven ATCC strains of bacteria were examined for apatite formation in a chemically-defined calcification-supporting medium and also in a metastable calcium phosphate solution. One, E. coli, calcified in both. One, S. aureus, calcified in the solution, but not in the medium. The other five did not calcify in either. Th...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345810600101001
更新日期：1981-10-01 00:00:00
abstract：:The aim of this study was to assess the effect of chewing gum containing phosphoryl oligosaccharides of calcium (POs-Ca) and a low concentration of fluoride (F) on the hardness of enamel subsurface lesions, utilizing a double-blind, randomized, and controlled in situ model. Fifteen individuals wore removable lingual a...
journal_title：Journal of dental research
pub_type： 杂志文章,随机对照试验
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更新日期：2012-04-01 00:00:00
abstract：:Pieces of fresh rat incisor enamel were extracted with various buffer solutions and prepared for electron microscopy. I observed partial or complete removal of organic constituents, shrinkage of rods and interrods, separation of stippled material from the enamel, and partial decalcification. The results modify the con...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：
更新日期：1982-12-01 00:00:00
abstract：:A plaque growth chamber was developed for long-term growth of five separate plaques from the same plaque or saliva sample under identical conditions of temperature and gas phase. Reagent addition and growth conditions for each plaque could be independently controlled, and each was accessible for sequential sampling an...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345910700110301
更新日期：1991-11-01 00:00:00
abstract：:Periodontal ligaments (PDLs) play an important role in remodeling the alveolar bond and cementum. Characterization of the periodontal tissue transcriptome remains incomplete, and an improved understanding of PDL features could aid in developing new regenerative therapies. Here, we aimed to generate and analyze a large...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034516645796
更新日期：2016-08-01 00:00:00
abstract：:Caries increment is affected by sugar-sweetened beverage (SSB) consumption. Taxing SSBs could reduce sugar consumption and caries increment. The authors aimed to estimate the impact of a 20% SSB sales tax on caries increment and associated treatment costs (as well as the resulting tax revenue) in the context of German...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034516660278
更新日期：2016-11-01 00:00:00
abstract：:The effects of Escherichia coli endotoxin upon mouse L929 cell proliferation, DNA synthesis, protein synthesis, and proline incorporation were determined. It was found that a level of endotoxin which inhibited cell proliferation prompted a similar inhibition of DNA synthesis and overall cell protein synthesis. In cont...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345790580061301
更新日期：1979-06-01 00:00:00
abstract：:The purpose of this investigation was to determine whether chlorhexidine combined with copper would exert a synergistic, growth-inhibitory effect against selected oral bacteria. Actinomyces viscosus, Actinomyces naeslundii, and Streptococcus mutans were all susceptible to chlorhexidine individually, with S. mutans dis...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345930720020901
更新日期：1993-02-01 00:00:00
abstract：:Previous studies have shown that fluoride affected the formation of apatite in spontaneous precipitations from unstable calcium phosphate solutions partly through its effect on the stability of such nonapatitic precursors as octacalcium phosphate, Ca8H2(PO4)6.5 H2O. The present study shows that fluoride had a comparab...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345800590021401
更新日期：1980-02-01 00:00:00
abstract：:The expired air of a group of 48 persons, 40 with and eight without dental amalgam restorations, was analyzed for its mercury content before and after chewing. Expired air samples were collected in polyethylene bags, and a known quantity of each was pumped into the mercury detector for measurement. The results showed ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345810600090601
更新日期：1981-09-01 00:00:00
abstract：:Different types of storage media have been studied for use in the replantation of exarticulated teeth. It was found that human periodontal ligament cells survived well in milk. Fifty percent of the cells were viable after 12 hours' storage in milk, while no cells were viable after three hours' storage in saliva. Brief...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345810600111301
更新日期：1981-11-01 00:00:00
abstract：UNLABELLED:We have previously shown that the resin monomer 2-hydroxyethylmethacrylate (HEMA) affects mouse B-lymphocyte activity, leading to increased IgG1 antibody production in vivo. In the present study, we tested, in vitro, the hypothesis that HEMA also affects human B-lymphocyte activity. The in vitro production o...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034510378522
更新日期：2010-12-01 00:00:00
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journal_title：Journal of dental research
pub_type： 杂志文章
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更新日期：2011-02-01 00:00:00
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journal_title：Journal of dental research
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journal_title：Journal of dental research
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更新日期：1982-12-01 00:00:00
abstract：:Samples of dental plaque obtained from the interproximal tooth surfaces of seven young men showed considerable variation in the prevalence and anatomic site distribution of the potentially cariogenic microorganism S mutans. Multiple application of 10% SnF2 topical solution and saline solution delivered to interproxima...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345770560010301
更新日期：1977-01-01 00:00:00
abstract：:Attempts to produce resin composite with antibacterial properties by incorporation of an antibacterial agent such as chlorhexidine have been reported, but problems can arise due to release of the inhibitory agent from the composite. Such problems may include toxic effects, influence on mechanical properties, and loss ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345940730080701
更新日期：1994-08-01 00:00:00
abstract：:The oral microbiota was compared between Romanian adolescents with a high prevalence of caries and no dental care and Swedish caries-active and caries-free adolescents in caries prevention programs and with a low prevalence of caries. Biofilm samples were analyzed by FLX+ pyrosequencing of the V1 to V4 hypervariable r...
journal_title：Journal of dental research
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更新日期：2016-01-01 00:00:00
abstract：:Occlusal alterations may result in changes in the functional performance of masticatory muscles. In this study, we set up an experimental model in rats to examine whether masticatory muscle abnormalities occur after a malocclusion is induced. Rats underwent unilateral amputation of the molar cusps to simulate an occlu...
journal_title：Journal of dental research
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更新日期：1999-11-01 00:00:00
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journal_title：Journal of dental research
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更新日期：2009-02-01 00:00:00
abstract：:Mutations and common polymorphisms in interferon regulatory factor 6 ( IRF6) are associated with both syndromic and nonsyndromic forms of cleft lip/palate (CLP). To date, much of the focus on this transcription factor has been on identifying its direct targets and the gene regulatory network in which it operates. Nota...
journal_title：Journal of dental research
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abstract：:Bone and dentin share similar biochemical compositions and physiological properties. Dentin, a major tooth component, is formed by odontoblasts; in contrast, bone is produced by osteoblasts. Osterix (Osx), a zinc finger-containing transcription factor, has been identified as an essential regulator of osteoblast differ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034514565647
更新日期：2015-03-01 00:00:00
abstract：:Intracystic fluid pressure is thought to be involved in odontogenic cyst growth. In this study, we investigated the effects of positive pressure on the expression of interleukin-1alpha (IL-1alpha), matrix metalloproteinases (MMPs), and prostaglandin E2 (PGE2) in odontogenic keratocysts to determine whether this pressu...
journal_title：Journal of dental research
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更新日期：2005-10-01 00:00:00
