Abstract:
:Calcitonin gene-related peptide (CGRP) plays a crucial role in the modulation of orofacial pain, and we hypothesized that CGRP mediated a neuron-glia crosstalk in orofacial pain. The objective of this study was to elucidate the mechanisms whereby CGRP mediated trigeminal neuron-glia crosstalk in modulating orofacial pain. Orofacial pain was elicited by ligating closed-coil springs between incisors and molars. Trigeminal neurons and satellite glial cells (SGCs) were cultured for mechanistic exploration. Gene and protein expression were determined through immunostaining, polymerase chain reaction, and Western blot. Orofacial pain was evaluated through the rat grimace scale. Our results revealed that the expressions of CGRP were elevated in both trigeminal neurons and SGCs following the induction of orofacial pain. Intraganglionic administration of CGRP and olcegepant exacerbated and alleviated orofacial pain, respectively. The knockdown of CGRP through viral vector-mediated RNA interference was able to downregulate CGRP expressions in both neurons and SGCs and to alleviate orofacial pain. CGRP upregulated the expression of inducible nitric oxide synthase through the p38 signaling pathway in cultured SGCs. In turn, L-arginine (nitric oxide donor) was able to enhance orofacial pain by upregulating CGRP expressions in vivo. In cultured trigeminal neurons, L-arginine upregulated the expression of CGRP, and this effect was diminished by cilnidipine (N-type calcium channel blocker) while not by mibefradil (L-type calcium channel blocker). In conclusion, CGRP modulated orofacial pain through upregulating the expression of nitric oxide through the p38 signaling pathway in SGCs, and the resulting nitric oxide in turn stimulated CGRP expression through N-type calcium channel in neurons, building a CGRP-mediated positive-feedback neuron-glia crosstalk.
journal_name
J Dent Resjournal_title
Journal of dental researchauthors
Liang H,Hu H,Shan D,Lyu J,Yan X,Wang Y,Jian F,Li X,Lai W,Long Hdoi
10.1177/0022034520950296subject
Has Abstractpub_date
2021-01-01 00:00:00pages
98-105issue
1eissn
0022-0345issn
1544-0591journal_volume
100pub_type
杂志文章abstract::In sham-operated and TPTX animals, F treatment did not affect final body weight, or serum Ca and P. In sham-treated rats, serum iPTH was not affected by F treatment. At the periosteum, F increased bone formation rate and bone and matrix apposition rates. At the endosteum, F treatment decreased forming surface, bone fo...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345770560031601
更新日期:1977-03-01 00:00:00
abstract::Chronic periodontitis is a multi-factorial disease involving anaerobic bacteria and the generation of an inflammatory response, including the production of metalloproteinases, pro-inflammatory cytokines, and eicosanoids. Hypochlorous acid (HOCl) and taurine-N-monochloramine (TauCl) are the end-products of the neutroph...
journal_title:Journal of dental research
pub_type: 杂志文章,评审
doi:10.1177/154405910408301101
更新日期:2004-11-01 00:00:00
abstract::Defects in the enamelin gene (ENAM) cause amelogenesis imperfecta (AI). Our objective was to identify the genetic etiology of enamel hypoplasia in a Caucasian proband. Our hypothesis was that ENAM was defective. The proband and his father have an AG insertion (g.13185_13186insAG; p.422FsX448) in ENAM previously identi...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034510365662
更新日期:2010-07-01 00:00:00
abstract::As a major intracellular degradation and recycling machinery, autophagy plays an important role in maintaining cellular homeostasis and remodeling during normal development. Our previous study showed that fluorapatite (FA) crystal-coated electrospun polycaprolactone (PCL) was capable of inducing differentiation and mi...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034516636852
更新日期:2016-06-01 00:00:00
abstract::Mucous membrane pemphigoid or cicatricial pemphigoid is a mucocutaneous blistering disease characterized by autoantibodies to different molecules in the basement membrane zone. Our objectives were to identify the target antigen recognized by sera from 20 untreated patients with pemphigoid disease limited to the oral c...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345010800080601
更新日期:2001-08-01 00:00:00
abstract::The reaction of the microvasculature in the periodontal ligament to mechanical pressure is considered a very important phenomenon with respect to the biological background to orthodontic tooth movement. For clarification of the microvascular reaction to mechanical pressure, an experimental model that incorporated a ha...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345920710060701
更新日期:1992-06-01 00:00:00
abstract::Setting reactions and compressive strengths of a self-hardening calcium phosphate cement (CPC) were investigated. The CPC consists of tetracalcium phosphate (TTCP) and anhydrous dicalcium phosphate (DCPA). The cement specimens were prepared by mixing 0.7 g of the powder (TTCP 72.9 wt% + DCPA 27.1 wt%) with 0.175 mL of...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345900690121201
更新日期:1990-12-01 00:00:00
abstract::A double-blind randomized clinical trial was performed in 6- to 7-yr-old schoolchildren to evaluate, in a 30-mo period, whether the caries increment on the distal surface of the second primary molars adjacent to permanent first molars sealed with fluoride release compounds would be lower with respect to those adjacent...
journal_title:Journal of dental research
pub_type: 杂志文章,随机对照试验
doi:10.1177/0022034514535808
更新日期:2014-07-01 00:00:00
abstract::Chemotherapeutics are presently considered for use in caries-preventive programs. The laboratory and in vivo testing of these agents, to some extent, parallels the methodology developed for the evaluation of fluoride products. However, fluoride is primarily effective by interfering with the de- and remineralization ba...
journal_title:Journal of dental research
pub_type: 杂志文章,评审
doi:10.1177/00220345940730031601
更新日期:1994-03-01 00:00:00
abstract::Tooth agenesis is one of the most common developmental anomalies affecting function and esthetics. The paired-domain transcription factor, Pax9, is critical for patterning and morphogenesis of tooth and taste buds. Mutations of PAX9 have been identified in patients with tooth agenesis. Despite significant progress in ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034517729322
更新日期:2018-02-01 00:00:00
abstract::Macrophage colony-stimulating factor (CSF-1) is a key regulatory cytokine for amelogenesis, and ameloblasts synthesize CSF-1. We hypothesized that PDGF stimulates DNA synthesis and regulates CSF-1 in these cells. We determined the effect of PDGF on CSF-1 expression using MEOE-3M ameloblasts as a model. By RT-PCR, MEOE...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910808700105
更新日期:2008-01-01 00:00:00
abstract::Epstein-Barr virus (EBV), in addition to its transforming properties, contributes to the pathogenesis of several inflammatory diseases. Here, we investigated its involvement in oral lichen planus (OLP), a common autoimmune-like disease of unknown etiopathogenesis that can display a malignant potential. EBV-infected ce...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034518788282
更新日期:2018-12-01 00:00:00
abstract::The tongue plays an important role in mastication and swallowing by its contact with the hard palate. Using an experimental palatal plate with 7 pressure sensors, and recording jaw movement using mandibular kinesiography, we assessed, in healthy subjects, the coordination of tongue and jaw movements during the entire ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910608500214
更新日期:2006-02-01 00:00:00
abstract::Medication-related osteonecrosis of the jaw (MRONJ), although initially believed to be exclusively associated with bisphosphonates, has been implicated in recent reports with additional drugs, especially the bone antiresorptive denosumab. The pathophysiology has not been fully elucidated, and no causal association bet...
journal_title:Journal of dental research
pub_type: 杂志文章,评审
doi:10.1177/0022034515572021
更新日期:2015-04-01 00:00:00
abstract::Attempts to produce resin composite with antibacterial properties by incorporation of an antibacterial agent such as chlorhexidine have been reported, but problems can arise due to release of the inhibitory agent from the composite. Such problems may include toxic effects, influence on mechanical properties, and loss ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345940730080701
更新日期:1994-08-01 00:00:00
abstract::A multiple logistic regression analysis was used to compute the odds ratios for 11 common occlusal features for asymptomatic controls (n = 147) vs. five temporomandibular disorder groups: Disc Displacement with Reduction (n = 81), Disc Displacement without Reduction (n = 48), Osteoarthrosis with Disc Displacement Hist...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345930720061301
更新日期:1993-06-01 00:00:00
abstract::Samples of dental plaque obtained from the interproximal tooth surfaces of seven young men showed considerable variation in the prevalence and anatomic site distribution of the potentially cariogenic microorganism S mutans. Multiple application of 10% SnF2 topical solution and saline solution delivered to interproxima...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345770560010301
更新日期:1977-01-01 00:00:00
abstract::This paper discusses loosely bound fluoride and its role in dental caries and prevention. Loosely bound fluoride (abbr. by Fa) is fluoride adsorbed onto enamel mineral crystallites. Several recent studies indicate that a high total level of fluoride in enamel does not guarantee protection against caries. This leads to...
journal_title:Journal of dental research
pub_type: 杂志文章,评审
doi:10.1177/00220345900690S118
更新日期:1990-02-01 00:00:00
abstract::Two independent cross-over studies investigated the possibility of enhanced early enamel lesion remineralization with the use of chewing gum. The first study involved a sorbitol-containing chewing gum, and the second, which had an identical protocol, tested a sucrose-containing chewing gum. In each study, 12 volunteer...
journal_title:Journal of dental research
pub_type: 临床试验,杂志文章
doi:10.1177/00220345920710120801
更新日期:1992-12-01 00:00:00
abstract::In the past two decades, accumulated evidence has clearly demonstrated the inhibitory effects of laser irradiation on enamel demineralization, but the exact mechanisms of these effects remain unclear. The purpose of this study was to investigate the effects of low-energy CO2 laser irradiation on demineralization of bo...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345000790091401
更新日期:2000-09-01 00:00:00
abstract::Squamous cell carcinoma (SCC) of the head and neck is the sixth most frequent cancer worldwide. The survival rate is among the lowest of the major cancers and has not improved significantly in the past two decades. Extensive local invasion and regional lymph node metastasis are, in large part, responsible for the poor...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345990780061001
更新日期:1999-06-01 00:00:00
abstract::Damage to the dentin matrix investigates the proliferation and mobilization of dental progenitor cells to the injury site, the mechanisms of which are not defined. EphB receptors and ephrin-B ligands expressed within the perivascular niche of dental pulp have been implicated following tooth injury. We propose that ele...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034509342363
更新日期:2009-09-01 00:00:00
abstract::Laser-Raman spectra of synthetic apatites containing from zero to 12.4 wt% carbonate ions and enamel apatite are reported. Special attention is paid to the correlation between the intensities of the carbonate, phosphate, and hydroxyl modes and the carbonate content in the apatites. The data presented here indicate tha...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345810600031601
更新日期:1981-03-01 00:00:00
abstract::A major extracellular protein of cultured adult human gingival fibroblasts was shown to be fibronectin by indirect immunofluorescence, lactoperoxidase catalyzed iodination and metabolic radiolabeling. Fibronectin preparations from these cells possessed the biological properties of agglutination of sheep red blood cell...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345800590031301
更新日期:1980-03-01 00:00:00
abstract::The Tp63 gene encodes for multiple isoforms of the p63 transcription factor, a member of the p53 family of proteins. Much like its more famous sibling, the biological role of p63 is quite complex, with wide-ranging effects on development, differentiation, and cellular lineage choices. The crucial function of p63 is ep...
journal_title:Journal of dental research
pub_type: 杂志文章,评审
doi:10.1177/0022034511411302
更新日期:2012-02-01 00:00:00
abstract::The aims of this work were (a) to assess the oral bioavailability of fluoride delivered from dentifrices and (b) to test for a possible link between the results and clinical data obtained with the same dentifrices. Oral fluoride concentrations were measured in samples of saliva and plaque taken from seven subjects, af...
journal_title:Journal of dental research
pub_type: 临床试验,杂志文章,评审
doi:10.1177/002203459207100S09
更新日期:1992-04-01 00:00:00
abstract::The effects of substituting gluconate for extracellular Cl, and of treatment with various ion transport blockers, on cytosol pH (pHi) and secretion by the acetylcholine stimulated rat mandibular gland were studied in vitro. Gluconate replacement increased pHi from 7.12 +/- 0.02 to 7.27 +/- 0.04, caused secretory rate ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345870660022401
更新日期:1987-02-01 00:00:00
abstract::Thermal and mechanical hypersensitivity in the injured region is a common complication. Although it is well known clinically that thermal and mechanical sensitivity of the oral mucosa is different from that of the skin, the mechanisms underlying injured pain of the oral mucosa remain poorly understood. The transient r...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034514565645
更新日期:2015-03-01 00:00:00
abstract::We explored the potential to diagnose Zika virus (ZIKV) infection by analyzing peptides in saliva during a convalescent phase of infection, long after resolution of acute disease. A 25-y-old woman clinically diagnosed with Zika fever in the first trimester was enrolled with her dizygotic twins for a 3-mo postnatal sam...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034517723325
更新日期:2017-09-01 00:00:00
abstract::It has been shown that a release of periosteal tension may augment the growth of a long bone; however, similar experiments in the mandible have produced equivocal results. For further definition of the role of periosteal tension in the growth of the mandibular condyle, 80 young female Sprague-Dawley rats were randomly...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345900690090201
更新日期:1990-09-01 00:00:00