Transglutaminase-2 regulation by arecoline in gingival fibroblasts.

Abstract:

:Transglutaminase-2 (TGM-2) stabilizes extracellular matrix (ECM) proteins by cross-linking and has been implicated in several fibrotic disorders. Arecoline present in betel quid has been proposed as one of the causative factors for oral submucous fibrosis (OSMF). Hence, we hypothesize that arecoline may regulate TGM-2 and may have a role in the pathogenesis of OSMF. The expression of TGM-2 was studied in OSMF tissues by real-time RT-PCR analysis, and significant overexpression was observed in most OSMF tissues (P=0.0112) compared with normal tissues. Arecoline induced TGM-2 mRNA and protein expression as well as TGM-2 activity in human gingival fibroblast cells. The addition of methocramine hemihydrate (M-2 muscarinic acetylcholine receptor selective antagonist) or 8'-bromo-cAMP abolished arecoline-mediated TGM-2 induction, suggesting a role for M-2 muscarinic acid receptor and a repressor role for cAMP. Our study provides evidence for TGM-2 overexpression in OSMF and its regulation by arecoline in oral fibroblasts.

journal_name

J Dent Res

authors

Thangjam GS,Agarwal P,Khan I,Verma UP,Balapure AK,Rao SG,Kondaiah P

doi

10.1177/0022034508329633

subject

Has Abstract

pub_date

2009-02-01 00:00:00

pages

170-5

issue

2

eissn

0022-0345

issn

1544-0591

pii

88/2/170

journal_volume

88

pub_type

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