Abstract:
:Fibroblast growth factor (FGF) signaling has been implicated in the regulation of osteogenesis in both intramembranous and endochondral ossifications. In the developing palate, the anterior bony palate forms by direct differentiation of cranial neural crest (CNC)-derived mesenchymal cells, but the signals that regulate the osteogenic cell fate in the developing palate remain unclear. In the present study, we investigated the potential role of FGF signaling in osteogenic fate determination of the palatal mesenchymal cells. We showed that locally activated FGF8 signaling in the anterior palate using a Shox2Cre knock-in allele and an R26RFgf8 allele leads to a unique palatal defect: a complete loss of the palatine process of the maxilla as well as formation of ectopic cartilaginous tissues in the anterior palate. This aberrant developmental process was accompanied by a significantly elevated level of cell proliferation, which contributes to an abnormally thickened palatal tissue, where the palatine process of the maxilla would normally form, and by a complete inhibition of Osterix expression, which accounts for the lack of bone formation. The coexpression of Runx2 initially with Sox9 and subsequently with Col II in the ectopic cartilaginous tissues indicates a conversion of osteogenic fate to a chondrogenic one. Consistent with the unique palatal phenotype, RNA-Sequencing analysis revealed that the augmented FGF8 signaling downregulated genes involved in ossification, biomineral tissue development, and bone mineralization but upregulated genes involved in cell proliferation, cartilage development, and cell fate commitment, which was further supported by quantitative real-time reverse transcription polymerase chain reaction validation of selected genes. Our results demonstrate that FGF8 signaling functions as a negative regulator of osteogenic fate and is sufficient to convert a subset of CNC cell-derived mesenchymal cells into cartilage in the anterior hard palate, which will have implications in future directed differentiation of CNC-derived precursor cells for clinical application.
journal_name
J Dent Resjournal_title
Journal of dental researchauthors
Xu J,Huang Z,Wang W,Tan X,Li H,Zhang Y,Tian W,Hu T,Chen YPdoi
10.1177/0022034517750141subject
Has Abstractpub_date
2018-05-01 00:00:00pages
589-596issue
5eissn
0022-0345issn
1544-0591journal_volume
97pub_type
杂志文章abstract::Early childhood caries (ECC) is a chronic disease affecting the oral health of children globally. This disease is multifactorial, but a primary factor is cariogenic microorganisms such as Streptococcus mutans. Biosynthetic gene clusters (BGCs) encode small molecules with diverse biological activities that influence th...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034520914519
更新日期:2020-07-01 00:00:00
abstract::Two series of dental composites, along with the unfilled resin matrix, were examined to determine the effects of filler level and size on selected properties. Both series were prepared by incorporating a silanated barium borosilicate filler into a visible-light-activated polyphenylene polymethacrylate resin matrix. On...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345850640121501
更新日期:1985-12-01 00:00:00
abstract::1. A method has been developed and described for obtaining accurate thermal diffusivity values for dental cements and other restorative materials. This method employs specimens of a short cylindrical geometry which are readily fabricated from simply-constructed molds. 2. Results obtained for polycarboxylate and glass-...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345810600120801
更新日期:1981-12-01 00:00:00
abstract::One of the main goals of bone tissue engineering is to identify and develop new biomaterials and scaffolds for structural support and controlled cell growth, which allow for formation or replacement of bone tissue. Recently, carbon nanotubes (CNT) have emerged as a potential candidate for bone tissue engineering. CNT ...
journal_title:Journal of dental research
pub_type: 杂志文章,评审
doi:10.1177/0022034513490957
更新日期:2013-07-01 00:00:00
abstract::Caries of the permanent first molars of 5-year-old children was found to be associated with caries of their diciduous teeth. The sample consisted of 220 children whose permanent first molars had erupted. They were selected out of 965 children who were a representative sample of children attending compulsory kindergart...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345760550062001
更新日期:1976-11-01 00:00:00
abstract::Greater fluoride concentrations were obtained in enamel treated with phosphate/fluoride solutions than in that treated with TiF4 at similar pH's (1.0) and F concentrations (0.6M F and 1.6M F). However, fluoride solution without added phosphate at pH 1.0 and 1.6M F concentration produced lower fluoride concentrations i...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345790580021001
更新日期:1979-02-01 00:00:00
abstract::Tooth agenesis is one of the most common developmental anomalies affecting function and esthetics. The paired-domain transcription factor, Pax9, is critical for patterning and morphogenesis of tooth and taste buds. Mutations of PAX9 have been identified in patients with tooth agenesis. Despite significant progress in ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034517729322
更新日期:2018-02-01 00:00:00
abstract::Seven oral doses of demethylchlortetracycline were administered to monkeys that received serial injections of lead acetate to intravitally stain calcification sites. Bone growth was greatly inhibited, whereas dentin apposition was spared from the cumulative toxicity of demethylchlortetracycline. Cessation of bone grow...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:
更新日期:1975-03-01 00:00:00
abstract::The discovery and implementation of fluoride in the prevention of dental caries is often praised as one of the most important achievements in health care. In the early 20th century, it took 30 y to identify fluoride as the cause of enamel mottling but also of reduced caries prevalence in a population drinking water co...
journal_title:Journal of dental research
pub_type: 历史文章,杂志文章
doi:10.1177/0022034519831604
更新日期:2019-07-01 00:00:00
abstract::As the use of adhesive restorative materials has increased during the last several years, interest in adhesive materials that release fluoride has also grown. The purpose of this study was to measure fluoride release from several adhesive restorative materials and to evaluate its effect on dentin resistance to deminer...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345990780101001
更新日期:1999-10-01 00:00:00
abstract::Protease-activated receptors (PARs) are G protein-coupled receptors, which are activated by proteolytical cleavage of the amino-terminus and act as sensors for extracellular proteases. We hypothesized that PAR-1 and PAR-2 can be modulated by inflammatory stimulus in human dental pulp cells. PAR-1 and PAR-2 gene expres...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034517719415
更新日期:2017-12-01 00:00:00
abstract::Wavelength-independent Microradiography (WIM), described in this paper, used polychromatic, high-energy (less than or equal to 60 kV) x-rays for determination of mineral concentrations in tooth material non-destructively. This was done with the aid of a reference step-wedge made of 94% aluminum, 6% zinc. The mass atte...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345900690081501
更新日期:1990-08-01 00:00:00
abstract::In 1986, the first nationwide epidemiological survey of adult oral health and attitudes to oral health care was carried out in The Netherlands. One of the aims of this study was to evaluate the prevalence and the quality (in terms of need for replacement) of amalgam and composite restorations in the Dutch adult popula...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345900690060901
更新日期:1990-06-01 00:00:00
abstract::Growth factors such as epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-alpha) play an important role in cell proliferation during embryogenesis. The purposes of the study were to characterize the expression patterns of EGF and TGF-alpha and their receptor, EGF receptor (EGF-R), and to analyze r...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345960750080301
更新日期:1996-08-01 00:00:00
abstract::Gingival overgrowth tissues have thickened connective tissue stroma, sometimes accompanied by the increased presence of collagen fibers, thickened epithelia, and elongated rete pegs. We have previously shown that expression of CCN2, also known as connective tissue growth factor (CTGF), correlates positively with the d...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034511404703
更新日期:2011-07-01 00:00:00
abstract::The aim of this study was to assess the changes occurring in subgingival biofilm composition and in the periodontal clinical parameters of subjects with periodontitis and type 2 diabetes mellitus (DM) treated by means of scaling and root planing (SRP) only or combined with systemic metronidazole (MTZ) and amoxicillin ...
journal_title:Journal of dental research
pub_type: 杂志文章,随机对照试验
doi:10.1177/0022034516639274
更新日期:2016-07-01 00:00:00
abstract::Enamel fluorosis can occur following either an acute or chronic exposure to fluoride during tooth formation. Fluorosed enamel is characterized by a retention of amelogenins in the early-maturation stage, and by the formation of a more porous enamel with a subsurface hypomineralization. The mechanisms by which fluoride...
journal_title:Journal of dental research
pub_type: 杂志文章,评审
doi:10.1177/00220345920710051701
更新日期:1992-05-01 00:00:00
abstract::In the past two decades, accumulated evidence has clearly demonstrated the inhibitory effects of laser irradiation on enamel demineralization, but the exact mechanisms of these effects remain unclear. The purpose of this study was to investigate the effects of low-energy CO2 laser irradiation on demineralization of bo...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345000790091401
更新日期:2000-09-01 00:00:00
abstract::The goal of this study was to assess the ability of a scintillation camera method to detect areas of active bone loss due to periodontitis. Technetium 99m methylene diphosphonate was used as the bone-seeking radiopharmaceutical. Bone-seeking radiopharmaceutical uptake (BSRU) was imaged and quantified in alveolar bone ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345910700010801
更新日期:1991-01-01 00:00:00
abstract::Previous studies have shown that therapeutic pulsed ultrasound (pulsed) has superior stimulatory effect on bone fracture healing compared with continuous ultrasound (continuous). Our predictive hypothesis was that pulsed ultrasound can produce better bone formation during mandibular osteodistraction than continuous ul...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910808701018
更新日期:2008-10-01 00:00:00
abstract::Two copper-free amalgams expanded excessively after storage in air at 60 degrees C for about five yr. One expanded significantly at 37 degrees C and slightly at 23 degrees C. High silver content, absence of copper, and presence of small amounts of zinc, or all three, seemed to promote very high expansions at 60 degree...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345820610063301
更新日期:1982-06-01 00:00:00
abstract::The pathophysiology of painful temporomandibular disorders is not fully understood, but evidence suggests that muscle pain modulates motor function in characteristic ways. This study tested the hypothesis that activation of nociceptive muscle afferent fibers would be linked to an increased excitability of the human ja...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910208100915
更新日期:2002-09-01 00:00:00
abstract::Dental epithelial progenitor cells differentiate into various cell types during development of tooth germs. To study this mechanism, we produced immortalized dental epithelial progenitor cells derived from the cervical-loop epithelium of a rat lower incisor. The expression patterns of cytokeratin 14, nerve growth fact...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910408300209
更新日期:2004-02-01 00:00:00
abstract::Conventional analyses of the cost-effectiveness of preventive regimens fail to account for the impact of attrition on reported costs. Using an economic model in which costs are allocated to fixed and variable components, and data from a recently completed clinical field trial, costs per participant are shown to vary w...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345820610050601
更新日期:1982-05-01 00:00:00
abstract::Using a pin-on-disc sliding-wear test apparatus, the wear behavior of a typical composite restorative vs. styli fashioned from human enamel, sintered alumina, and magnesium fluoride was examined. The rates of restorative wear were found to be identical, within the experimental error of the measurements, for tests usin...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345840630062501
更新日期:1984-06-01 00:00:00
abstract::Amylase activity in various tissues--i.e., submandibular/sublingual and parotid glands, the pancreas, saliva, and serum--in rats was measured after injection of isoproterenol, pilocarpine, and acetylcholine. All agents reduced amylase activity in the parotid gland and increased the enzyme activity in the submandibular...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345830620011201
更新日期:1983-01-01 00:00:00
abstract::Potassium ions in dentifrices for treating 'hypersensitive' dentin are believed to act directly on intradental nerves by raising extracellular potassium ion concentration ([K+]o) sufficiently to prevent action potential generation by axonal accommodation. However, the [K+]o necessary to block nerve conduction is not p...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345950740020301
更新日期:1995-02-01 00:00:00
abstract::The present study was conducted to investigate the role of proteolysis by matrix metalloproteinase 20 (MMP20) in regulating the initial formation of the enamel mineral structure during the secretory stage of amelogenesis, utilizing Mmp20-null mice that lack this essential protease. Ultrathin sagittal sections of maxil...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034518823537
更新日期:2019-04-01 00:00:00
abstract::1) A new adhesive opaque resin containing a reactive monomer, 4-methacryloxy-ethyl trimellitate anhydride (4-META), was prepared, and its application to thermosetting acrylic resin veneer crowns was studied. 2) The 4-META opaque resin was applied to a variety of nickel-chromium dental alloy specimens which had undergo...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345810600091101
更新日期:1981-09-01 00:00:00
abstract::Recent evidence implicates endothelin in nociception, but it is unclear how endothelin activates trigeminal ganglion (TRG) neurons. In the present study, we investigated the expression of the endothelin receptors ETA and ETB and endothelin-induced responses in rat TRG neurons. Double-immunofluorescence studies demonst...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034513478428
更新日期:2013-04-01 00:00:00