Abstract:
:A predominant form of the GABAA/benzodiazepine receptor-Cl- channel complex is believed to consist of three different 48-55 kDa subunits (alpha, beta, gamma) with unknown stoichiometry. Plasmids containing the rat GABAA receptor cDNAs coding for alpha 1, beta 2, and gamma 2 were co-transfected, along with a plasmid encoding G418 resistance, into human embryonic kidney cells previously transformed with Adenovirus 5 (HEK-293) [J. Gen. Virol., 36 (1977) 59-72]. Four percent of the G418 resistant colonies were found to express mRNA for all three of the GABAA subunits constitutively. A single cell clone derived from one of the alpha 1 beta 2 gamma 2 expressors has demonstrated stable electrophysiological characteristics over 25 passages. The GABA-activated Cl- current in this cell line is blocked by picrotoxin and bicuculline, and is modulated by a variety of agonist and inverse agonist ligands including diazepam, Ro 154513, zolpidem, and beta-CCE. The cell line has been used successfully over a 12-month period as a screen for novel drugs modulating GABA-mediated polarization of neuronal cells.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Hamilton BJ,Lennon DJ,Im HK,Im WB,Seeburg PH,Carter DBdoi
10.1016/0304-3940(93)90323-dsubject
Has Abstractpub_date
1993-04-30 00:00:00pages
206-9issue
2eissn
0304-3940issn
1872-7972pii
0304-3940(93)90323-Djournal_volume
153pub_type
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journal_title:Neuroscience letters
pub_type: 杂志文章
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pub_type: 杂志文章,收录出版
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pub_type: 杂志文章,随机对照试验
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更新日期:2008-08-08 00:00:00
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journal_title:Neuroscience letters
pub_type: 杂志文章
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更新日期:2005-09-23 00:00:00
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journal_title:Neuroscience letters
pub_type: 杂志文章
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journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(01)02088-2
更新日期:2001-09-07 00:00:00
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journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(89)90824-0
更新日期:1989-12-15 00:00:00
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journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2020.134984
更新日期:2020-05-29 00:00:00
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journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(93)90610-w
更新日期:1993-08-06 00:00:00