Rapid identification of polymorphic CA-repeats in YAC clones.

abstract：:To date only a few neutralizing antibodies against HIV-1 exist. Since these neutralizing antibodies are only rarely found in sera of HIV-1 infected individuals an active vaccine is required. We recently developed murine anti-idiotypic antibody Ab2/3H6 against monoclonal antibody (mAb) 2F5, which is one of the most pro...

journal_title：Molecular biotechnology

authors： Gach JS,Quendler H,Ferko B,Katinger H,Kunert R

更新日期：2008-06-01 00:00:00

abstract：:Nitrilases have attracted tremendous attention for the preparation of optically pure carboxylic acids. This article aims to address the production and utilization of a highly enantioselective nitrilase from Pseudomonas putida MTCC 5110 for the hydrolysis of racemic mandelonitrile to (R)-mandelic acid. The nitrilase ge...

journal_title：Molecular biotechnology

authors： Banerjee A,Dubey S,Kaul P,Barse B,Piotrowski M,Banerjee UC

更新日期：2009-01-01 00:00:00

abstract：:Jet-injection has become an applicable technology among other established nonviral delivery systems, such as particle bombardment or in vivo electroporation. The low-volume jet injector employed in this study uses compressed air to inject solutions of 1.5-10 microL containing naked DNA into the desired tissue. The nov...

journal_title：Molecular biotechnology

authors： Walther W,Stein U,Fichtner I,Voss C,Schmidt T,Schleef M,Nellessen T,Schlag PM

更新日期：2002-06-01 00:00:00

abstract：:Artificial microRNA (amiRNA) has become a powerful tool for gene silencing in plants. A new method for easy and rapid construction of rice artificial miRNA vector is described. The procedure involved modification of the pCAMBIA1300-UR vector by insertion of a 'vector modification fragment'. This was prepared from the ...

journal_title：Molecular biotechnology

authors： Wang X,Yang Y,Yu C,Zhou J,Cheng Y,Yan C,Chen J

更新日期：2010-11-01 00:00:00

abstract：:Hereditary cancers account for approximately 10 % of breast and ovarian cancers. Mutations of the BRCA1 and BRCA2 genes, encoding two proteins involved in DNA repair, underlie most cases of such hereditary cancers. Women with BRCA mutations develop breast cancer in 50-80 % of cases and ovarian cancer in 10-40 % of cas...

journal_title：Molecular biotechnology

authors： Vuttariello E,Borra M,Calise C,Mauriello E,Greggi S,Vecchione A,Biffali E,Chiappetta G

更新日期：2013-07-01 00:00:00

abstract：:Costimulatory signal(s) in addition to engagement of T cell receptor is important for optimal activation of T lymphocytes. During last decade several dozens of new costimulatory molecules, including 4-1BB, CD40, OX40, CD27, and ICOS, have been identified. It is likely that in Post-Genome Era more molecules with potent...

journal_title：Molecular biotechnology

authors： Chapoval AI,Zhu G,Chen L

更新日期：2002-07-01 00:00:00

abstract：:The simple use of nonisotopic hybridization probes to detect complementary sequences provides valuable information in a large number of research and commercial applications. In hybridization assays, the four "S's (speed, simplicity, sensitivity, and specificity) are important criteria for determining the choice of pro...

journal_title：Molecular biotechnology

authors： Ruth JL

更新日期：1996-10-01 00:00:00

abstract：:Classic toxicology studies often utilize in vivo animal models. Newer approaches employing in vitro organ-specific cellular models have been developed in recent years to help accelerate the speed and reduce the cost of traditional toxicology testing. Toward the goal of supporting in vitro cellular model research with ...

journal_title：Molecular biotechnology

authors： Mossoba ME,Vohra SN,Bigley E 3rd,Sprando J,Wiesenfeld PL

更新日期：2020-04-01 00:00:00

abstract：:Oenococcus oeni is the main bacterial species that drives malolactic fermentation in wine. Most O. oeni strains produce capsular exopolysaccharides (EPS) that may contribute to protect them in the wine hostile environment. In O. oeni genome sequences, several genes are predicted to encode priming glycosyltransferases ...

journal_title：Molecular biotechnology

authors： Dimopoulou M,Claisse O,Dutilh L,Miot-Sertier C,Ballestra P,Lucas PM,Dols-Lafargue M

更新日期：2017-08-01 00:00:00

abstract：:Adenoviral dodecahedron (Dd) is a virus-like particle composed of twelve pentameric penton base (Pb) proteins, responsible for adenovirus cell penetration. It is generated spontaneously in the baculovirus system upon expression of the Pb gene of adenovirus serotype 3. This particle shows remarkable cell penetration ab...

journal_title：Molecular biotechnology

authors： Szurgot I,Jedynak M,Podsiadla-Bialoskorska M,Piwowarski J,Szolajska E,Chroboczek J

更新日期：2015-06-01 00:00:00

abstract：:The biosensor field has grown enormously since the first demonstration of the biosensor concept by Leland C. Clark, Jr. in 1962. Today's biosensor market is dominated by glucose biosensors, mass-produced enzyme electrodes for the rapid self-diagnosis of blood glucose levels by diabetes sufferers. Here we take a histor...

journal_title：Molecular biotechnology

authors： Newman JD,Setford SJ

更新日期：2006-03-01 00:00:00

abstract：:Lactic acid bacteria (LAB) are functional starter cultures in food and dairy industry and are also regarded as power houses for bioprocess and fermentation technology. Due to extensive applications in food and medical applications, intensive research and developmental activities are going on throughout the world to un...

journal_title：Molecular biotechnology

authors： Vivek N,Hazeena SH,Rajesh RO,Godan TK,Anjali KB,Nair LM,Mohan B,Nair SC,Sindhu R,Pandey A,Binod P

更新日期：2019-08-01 00:00:00

abstract：:RNA interference methodology suppresses specific gene expression, thus mimicking loss-of-function mutation and enabling in vitro and in vivo gene function analysis. Lipofectamine RNAiMAX, a new transfection reagent, has been confirmed high efficiency in delivering small interfering RNA (siRNA) into mesenchymal stem ce...

journal_title：Molecular biotechnology

authors： Zhao M,Yang H,Jiang X,Zhou W,Zhu B,Zeng Y,Yao K,Ren C

更新日期：2008-09-01 00:00:00

abstract：:Technological achievements in the last 5 to 10 yr and their application to sequencing and polymorphism discovery in the human genome have fostered a renewed interest in the genetic basis of drug response. Consequently, the field of pharmacogenetics/pharmacogenomics has been gaining momentum, fueled not only on technol...

journal_title：Molecular biotechnology

authors： McCarthy JJ

更新日期：2003-11-01 00:00:00

abstract：:High-quality RNA preparations are critical for further applications such as reverse transcriptase-polymerase chain reaction (RT-PCR) transcript amplifications, and elaboration of cDNA and expressed sequence tag libraries. Melanins are phenolic compounds present in many fungi and apparently play key roles in fungi path...

journal_title：Molecular biotechnology

authors： Islas-Flores I,Peraza-Echeverría L,Canto-Canché B,Rodríguez-García CM

更新日期：2006-09-01 00:00:00

abstract：:In this paper, we report cloning of a pectate lyase gene from Bacillus amyloliquefaciens S6 (pelS6), and biochemical characterization of the recombinant pectate lyase. PelS6 was found to be identical with B. subtilis 168 pel enzyme with 100% amino acid sequence homology. Although these two are genetically very close, ...

journal_title：Molecular biotechnology

authors： Bekli S,Aktas B,Gencer D,Aslim B

更新日期：2019-09-01 00:00:00

abstract：:Fibrillation of α-synuclein proceeds through distinct stages, with oligomers combining to form the seed or the nucleus, followed by exponential and saturation phases. Osmolytes are considered to act as protein stabilizers by virtue of their ability to inhibit protein aggregation. Trehalose, a non-reducing disaccharide...

journal_title：Molecular biotechnology

authors： Naik V,Kardani J,Roy I

更新日期：2016-04-01 00:00:00

abstract：:Lactic acid bacteria (LAB) are generally sensitive to hydrogen peroxide (H(2)O(2)), Lactobacillus sakei YSI8 is one of the very few LAB strains able to degrade H(2)O(2) through the action of a heme-dependent catalase. Lactobacillus rhamnosus strains are very important probiotic starter cultures in meat product ferment...

journal_title：Molecular biotechnology

authors： An H,Zhou H,Huang Y,Wang G,Luan C,Mou J,Luo Y,Hao Y

更新日期：2010-06-01 00:00:00

abstract：:Terminal Restriction Fragment Length Polymorphism (T-RFLP) or Fluorescent Polymerase Chain Reaction/Restriction Fragment Length Polymorphism (FluRFLP) have made a significant impact on the way in which PCR products amplified from mixed community DNA extracts have been assessed. Technically, these approaches are essent...

journal_title：Molecular biotechnology

authors： Bruce KD,Hughes MR

更新日期：2000-11-01 00:00:00

abstract：:The extracellular signal-regulated kinase (ERK) cascade is a central intracellular signaling pathway that is activated by a variety of extracellular stimuli, and thereby regulates cellular processes such as proliferation, differentiation, and oncogenic transformation. To execute these functions, the signals of those s...

journal_title：Molecular biotechnology

authors： Chuderland D,Seger R

更新日期：2005-01-01 00:00:00

abstract：:Angiogenesis has been considered an important target for cancer therapy. The inhibition of angiogenesis represents a promising strategy for anti-cancer treatment, tumor growth inhibition, and metastasis. Vasostatin 30 (Vs30), and the 14.1 kDa vasoinhibin (Vi-II-14.1) are two peptides with remarkable anti-tumor and ant...

journal_title：Molecular biotechnology

authors： Vazquez Rodriguez G,Gonzalez C,De Leon Rodriguez A

更新日期：2013-07-01 00:00:00

abstract：:DNA damage is one of the most impactful events in living organisms, leading to DNA sequence changes (mutation) and disruption of biological processes. A study has identified a protein called Damage Suppressor Protein (Dsup) in the tardigrade Ramazzotius varieornatus that has shown to reduce the effects of radiation da...

journal_title：Molecular biotechnology

authors： Kirke J,Jin XL,Zhang XH

更新日期：2020-12-01 00:00:00

abstract：:Brn-3a, a member of the POU gene family (so-called because of the similarity with the group of transcription factors Pit, Oct, and Unc), was found in neuronal cells engaged in the transcription activity of the p1 and p2 promoters of the most powerful antiapoptotic gene, namely, Bcl-2. The alternative splicing of Brn-3...

journal_title：Molecular biotechnology

authors： Chiarugi V,Del Rosso M,Magnelli L

更新日期：2002-10-01 00:00:00

abstract：:This work describes protocols for the production of single-chain antibody and T-cell receptor fragments in E. coli. A choice of methods is given for the purification of the recombinant fragments that rely on the use of either immunoaffinity or metal chelate affinity chromatography. The TCR fragments may have to be den...

journal_title：Molecular biotechnology

authors： Molloy PE,Graham BM,Cupit PM,Grant SD,Porter AJ,Cunningham C

更新日期：1995-12-01 00:00:00

abstract：:Human immunodeficiency virus types 1 and 2 (HIV-1, -2), the etiological agents of AIDS, are retroviruses that replicate in CD4+ T lymphocytes, monocytes, and macrophages. Early anti-HIV therapies were directed at the step in the virus life cycle that was considered the most readily blocked: the transcription of viral ...

journal_title：Molecular biotechnology

authors： Patience C,Moore J,Boyd M

更新日期：1994-02-01 00:00:00

abstract：:Human recombinant vascular endothelial growth factor-A121 (hrVEGF-A121) has applications in pharmaceutical industry especially in regenerative medicine. Here, we report the expression, purification, and characterization of hrVEGF-A121 in Escherichia coli expression system using human small ubiquitin-related modifier-3...

journal_title：Molecular biotechnology

authors： Samuel RVM,Farrukh SY,Rehmat S,Hanif MU,Ahmed SS,Musharraf SG,Durrani FG,Saleem M,Gul R

更新日期：2018-08-01 00:00:00

abstract：:Tea, a beverage crop, is a rich source of polyphenols and polysaccharides which greatly attribute to its importance. However, oxidation and precipitation of these compounds during nucleic acids extraction is a limitation to molecular biology and genomic studies. On isolation of total RNA from root tissue using establi...

journal_title：Molecular biotechnology

authors： Muoki RC,Paul A,Kumari A,Singh K,Kumar S

更新日期：2012-09-01 00:00:00

abstract：:Lentiviral transduction is a highly efficient DNA delivery method for RNA interference applications. However, obtaining high lentiviral titers of shRNA and miRNA encoding vectors is challenging, since shRNA and miRNA cassettes have been shown to reduce lentiviral titers. In this study, we compare four commercially ava...

journal_title：Molecular biotechnology

authors： Albrecht C,Hosiner S,Tichy B,Aldrian S,Hajdu S,Nürnberger S

更新日期：2015-06-01 00:00:00

abstract：:Amplification of DNA or RNA sequences using the polymerase chain reaction (PCR) or reverse transcriptase PCR (RT-PCR) requires primers of an appropriate length to be designed. Two hexamer restriction primers, denoted as E101 and H301, which correspond to sequences of EcoRI and HindIII recognition sites, respectively, ...

journal_title：Molecular biotechnology

authors： Ryu KH,Choi SH,Lee JS

更新日期：2000-01-01 00:00:00

abstract：:Identification of expressed sequence tags (ESTs) in large genomic segments is an important step in positional cloning and genomic mapping studies. A simple and efficient polymerase chain reaction (PCR)-based approach is described here to identify coding sequences in large genomic fragments of DNA cloned in vectors suc...

journal_title：Molecular biotechnology

authors： Parimoo S

更新日期：1997-12-01 00:00:00