Abstract：
:In this paper, we report cloning of a pectate lyase gene from Bacillus amyloliquefaciens S6 (pelS6), and biochemical characterization of the recombinant pectate lyase. PelS6 was found to be identical with B. subtilis 168 pel enzyme with 100% amino acid sequence homology. Although these two are genetically very close, they are distinctly different in physiology. pelS6 gene encodes a 421-aa protein with a molecular mass of 65,75 kDa. Enzyme activity increased from 12.8 ± 0.3 to 49.6 ± 0.4 units/mg after cloning. The relative enzyme activity of the recPel S6 ranged from 80% to 100% at pH between 4 and 14. It was quite stable at different temperature values ranging from 15 to 90 °C. The recPEL S6 showed a maximal activity at pH 10 and at 60 °C. 0.5 mM of CaCl2 is the most effective metal ion on the recPEL S6 as demonstrated by its increased relative activity with 473%. recPEL S6 remained stable at - 20 °C for 18 months. In addition recPEL S6 increased juice clarity. This study introduces a novel bacterial pectate lyase enzyme with its characteristic capability of being highly thermostable, thermotolerant, and active over a wide range of pH, meaning that it can work at both acidic and alkaline environments, which are the most preferred properties in the industry.
journal_name
Mol Biotechnoljournal_title
Molecular biotechnologyauthors
Bekli S,Aktas B,Gencer D,Aslim Bdoi
10.1007/s12033-019-00194-2subject
Has Abstractpub_date
2019-09-01 00:00:00pages
681-693issue
9eissn
1073-6085issn
1559-0305pii
10.1007/s12033-019-00194-2journal_volume
61pub_type
杂志文章abstract：:Internal ribosome entry site (IRES) elements are highly structured RNA sequences that function to recruit ribosomes for the initiation of translation. In contrast to the canonical cap-binding, the mechanism of IRES-mediated translation initiation is still poorly understood. Translation initiation of the coxsackievirus...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s12033-013-9674-4
更新日期：2013-10-01 00:00:00
abstract：:Artificial microRNA (amiRNA) has become a powerful tool for gene silencing in plants. A new method for easy and rapid construction of rice artificial miRNA vector is described. The procedure involved modification of the pCAMBIA1300-UR vector by insertion of a 'vector modification fragment'. This was prepared from the ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-010-9291-4
更新日期：2010-11-01 00:00:00
abstract：:IgG class antibodies express catalytic activities rarely and at very low levels. Here, we studied polyclonal IgA and IgG preparations from healthy human sera and saliva for the ability to hydrolyze model peptidyl-aminomethylcoumarin (peptide-AMC) substrates. These substrates permit objective evaluation of the catalyti...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-007-0003-7
更新日期：2007-06-01 00:00:00
abstract：:De novo design provides an in silico toolkit for the design of novel small molecular structures to a set of specified structural constraints. With the avalanche of bioinformatics data, de novo design is ideally suited for exploring molecules that could be useful for chemical genomics. The design process involves manip...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s12033-007-0037-x
更新日期：2007-11-01 00:00:00
abstract：:Low molecular weight RNA (LMW RNA) is generally obtained either from the total RNA or from total nucleic acids solution. Many steps and chemical reagents are involved in traditional methods for LMW RNA isolation where degradation of LMW RNA often occurs, especially for plant materials with high levels of secondary cat...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-009-9204-6
更新日期：2010-01-01 00:00:00
abstract：:A duplex real-time PCR assay with melting curve analysis, using the EvaGreen fluorescence dye, was developed for rapid and reliable identification of bovine and caprine in ruminant feeds. The method merges the use of bovine (Bos taurus) and caprine (Capra hircus) specific primers that amplify small fragments (bovine 9...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-014-9756-y
更新日期：2014-08-01 00:00:00
abstract：:Pyrococcus woesei (DSM 3773) beta-galactosidase gene amplified by polymerase chain reaction was cloned into KpnI and HindIII binding sites of pET-30LIC expression plasmid. The obtained pGal2 (6785 bp) transcription vector was then transferred to Escherichia coli B121 (DE3) cells. High identity (99.9%) of DNA sequences...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02740841
更新日期：1998-12-01 00:00:00
abstract：:A gene encoding maltogenic amylase from acidic Bacillus sp. US149 (maUS149) was cloned, sequenced and over-expressed in Escherichia coli. The nucleotide sequence analysis revealed an open reading frame (ORF) of 1749 bp encoding a protein of 582 residues. The alignment of deduced amino acid sequence revealed a relative...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-007-9017-4
更新日期：2008-03-01 00:00:00
abstract：:Derivation of cardiomyocytes directly from patients' own fibroblasts could offer a new therapeutic approach for those with ischemic heart disease. An essential step toward clinical application is to establish safe conversion of human fibroblasts into a cardiac fate. Here we aimed to efficiently and safely generate car...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-017-0007-x
更新日期：2017-06-01 00:00:00
abstract：:D-Glucosamine is a commonly used dietary supplement that promotes cartilage health in humans. Metabolic flux analysis showed that D-glucosamine production could be increased by blocking three pathways involved in the consumption of glucosamine-6-phosphate and acetylglucosamine-6-phosphate. By homologous single-exchang...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-020-00257-9
更新日期：2020-08-01 00:00:00
abstract：:In this study, host-specific forms of the blast pathogen Magnaporthe oryzae in sub-Saharan Africa (SSA) were characterised from distinct cropping locations using a combination of molecular and biological assays. Finger millet blast populations in East Africa revealed a continuous genetic variation pattern and lack of ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-011-9429-z
更新日期：2012-02-01 00:00:00
abstract：:The low economic profits of feather recycling lead that the large amount of feathers is currently discarded in China. To convert feather hydrolysates into GSH with high values, surface display of the bifunctional glutathione synthetase encoded by gcsgs from Streptococcus thermophilus on Saccharomyces cerevisiae and th...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-014-9750-4
更新日期：2014-08-01 00:00:00
abstract：:The advent of recombinant antibody technology led to an enormous revival in the use of antibodies as diagnostic and therapeutic tools for fighting cancer. This review provides a brief historical sketch of the development of recombinant antibodies for the diagnosis and immunotherapy of cancer and summarizes the most si...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1385/MB:25:1:1
更新日期：2003-09-01 00:00:00
abstract：:PREP1/p160 is a protein complex with relevant physiopathological roles in vivo. p160 regulates PREP1 transcriptional activity by preventing the formation of other PREP1-containing complexes, whereas PREP1 regulates p160 activity by increasing its stability. This induces the repression of the insulin-regulated glucose ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-016-9932-3
更新日期：2016-05-01 00:00:00
abstract：:The MYB proteins constitute one of the largest transcription factor families in plants. Much research has been performed to determine their structures, functions, and evolution, especially in the model plants, Arabidopsis, and rice. However, this transcription factor family has been much less studied in wheat (Triticu...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-011-9486-3
更新日期：2012-10-01 00:00:00
abstract：:Isolation of RNA from recalcitrant tree tissues has been problematic due to large amounts of secondary metabolites and interfering compounds in their cells. We have developed an efficient RNA extraction method, which yielded high-quality RNA preparations from tissues of the lychee tree. The method reported here utiliz...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-007-0073-6
更新日期：2008-01-01 00:00:00
abstract：:The DNA molecular analyses together with ampelography, ampelometry, and biochemistry are essential for grapevine identification and investigation of genetic differences among the Vitis vinifera L. cultivars and clones. Ten Malvasia cultivars (i.e., Istrian Malvasia; M. delle Lipari; M. bianca di Candia; M. di Candia A...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-011-9423-5
更新日期：2012-03-01 00:00:00
abstract：:In this study, we investigated expression and dimerization of an ER-associated degradation (ERAD) substrate, a null Hong Kong variant of α-1-antitrypsin (NHK) using immunoblotting assay and a novel NanoLuc complementary reporter system called the NanoBiT (NB) assay. This NB-tagged NHK made it possible to monitor the i...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-018-0092-5
更新日期：2018-08-01 00:00:00
abstract：:This review outlines the various methods available for submitting sequence data to the EMBL Nucleotide Sequence Database. Depending on the type of sequence data and the facilities available to the submitter, one method may be more suitable than another. Recent developments have been the World Wide Web submission tool,...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/BF02762322
更新日期：1996-08-01 00:00:00
abstract：:The simple use of nonisotopic hybridization probes to detect complementary sequences provides valuable information in a large number of research and commercial applications. In hybridization assays, the four "S's (speed, simplicity, sensitivity, and specificity) are important criteria for determining the choice of pro...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/BF02740771
更新日期：1996-10-01 00:00:00
abstract：:The term "coincidence cloning" encompasses a wide range of methodologies, the aim of which is to isolate DNA sequences which occur in both of two input DNA sources. The nature of these input DNAs may be genomic or cDNA, cloned or uncloned, and as such the far reaching applicability of the techniques can be imagined. I...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/BF02900362
更新日期：1996-06-01 00:00:00
abstract：:Characterization of novel plant gene promoters underpins the development of transgenic crop plants. Here, we report a novel 5' regulatory sequence (Eriubi D7) of the ubiquitin gene from Erianthus arundinaceus, a wild relative of sugarcane resistant to many biotic and abiotic stresses. A 3.2-kb regulatory sequence of u...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-015-9875-0
更新日期：2015-09-01 00:00:00
abstract：:Cardiomyocytes are terminally differentiated cells that to date have been characterized as poor targets for nonviral gene transfer. This study was therefore designed to determine the optimal nonviral gene transfer parameters in cell cultures of neonatal rat cardiomyocytes and to compare them with the efficiency of gen...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:28:1:21
更新日期：2004-09-01 00:00:00
abstract：:HOX genes are highly conserved transcription factors that determine the identity of cells and tissues along the anterior-posterior body axis in developing embryos. Aberrations in HOX gene expression have been shown in various tumors. However, the correlation of HOX gene expression patterns with tumorigenesis and cance...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-013-9682-4
更新日期：2014-01-01 00:00:00
abstract：:RNA editing is one of the post-transcriptional RNA processes. RNA editing generates RNA and protein diversity in eukaryotes and results in specific amino acid substitutions, deletions, and changes in gene expression levels. Adenosine-to-inosine RNA editing represents the most important class of editing in human and af...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s12033-012-9498-7
更新日期：2012-09-01 00:00:00
abstract：:Optimization of gene coding-sequence, including preferred codon usage and removal of cryptic splice sites and mRNA-destabilizing motifs, has been shown to improve recombinant protein production of different proteins. Here, we present data to show that gene optimization can also be used to improve the production of a c...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1385/MB:34:2:151
更新日期：2006-10-01 00:00:00
abstract：:Biobanks, more formally known as biological resource centers (BRCs), form an "unsung" yet critical component of the infrastructures for scientific research, industry and conservation, without which much of the current scientific activity involving microbial cultures and cell-lines would be effectively impossible. BRCs...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s12033-008-9099-7
更新日期：2008-10-01 00:00:00
abstract：:Genes are recognized as undergoing genomic imprinting when they are capable of being expressed only from the paternal or only from the maternal chromosome. The process can occur coordinately within large physical domains in mammalian chromosomes. One interesting facet of the study of genomic imprinting is that it offe...
journal_title：Molecular biotechnology
pub_type： 杂志文章,评审
doi：10.1007/BF02915809
更新日期：1999-04-01 00:00:00
abstract：:Podophyllotoxin (PPT) is a plant natural product that serves as a precursor for the synthesis of many well-known chemotherapeutic drugs. The limited availability and high demand for the source plants of PPT have led to the exploration of alternative sources for this compound. In this study, we utilized the endophytic ...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-015-9888-8
更新日期：2015-12-01 00:00:00
abstract：:Previous studies have shown that mRNA-electroporated dendritic cells (DCs) are able to process and present tumor-associated antigens, leading to the activation of tumor-specific T cells in vitro and in vivo. However, the optimal maturation state of antigen loading and half-life of the mRNA-translated protein product a...
journal_title：Molecular biotechnology
pub_type： 杂志文章
doi：10.1007/s12033-008-9071-6
更新日期：2008-10-01 00:00:00
